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Offlineoculodextro
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Condensation leading to slime mold? [Re: Mindgmz]
    #26636880 - 04/29/20 01:40 PM (3 years, 8 months ago)

Hello,

I keep running into an issue with pasty-whites no pour tek. I get this flat grayish growth around the edges of my plates. Seems to be where the condensation ends up sitting. I invert my jars so the water should leak to lid, but I still end up with a flat growth. (appears to be slime mold?)








Apologize for shit images!


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OfflineStargirl
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Registered: 04/29/20
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26637294 - 04/29/20 05:29 PM (3 years, 8 months ago)

Hi,

This is my first time using this forum. Looking for some feedback from experienced mushroom cultivation experts. I am giving mushroom cultivation a go for the first time and I have chosen the P. Cubensis strain (Golden Teacher) as I hear it's best for beginners. I am using the PFtek method and I have been taking many sterilization precautions, such as boiling the substrate, inoculation using the oven door method, and thorough sterilization of the spore syringe as well as micropore tape. I inoculated my cakes a week ago and have begun to see some results as far as cultivation. I haven't noticed any discoloration but i'm not quite sure exactly what healthy mycelium should be looking like at this stage. I wanna be extra careful that I don't try to fruit any contaminated cakes. Any feedback would be greatly appreciated!!! I will include some pictures of my jars. Hope anyone reading this is having a good day!





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OfflineCrackatoa
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Registered: 03/31/19
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Re: Recognizing and dealing with contamination [Re: Stargirl]
    #26637335 - 04/29/20 05:52 PM (3 years, 8 months ago)

Looking good so far star :thumbup:


--------------------

Crack's Tek's and Shit



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Offlinenevereataamanita


Registered: 09/08/19
Posts: 121
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Re: Recognizing and dealing with contamination [Re: Crackatoa]
    #26637357 - 04/29/20 06:06 PM (3 years, 8 months ago)

I've got this white points of grainy contamination (?) showing up around the mycelium on a couple of plates (from like 9:00 to 3:00 on this plate). Is it yeast? Interesting that it appears to be associated with the myc and not around the margins of the plate. Also, it only showed up in plates of this culture and not of the three other cultures I did transfers of in the same SAB session (so I'm assuming it came along from the original donor).


Can I wait for it to grow out and make sure I don't transfer from the portion of the plate that has this crap? Or if I want to move this myc along do I need to transfer now?


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My LAGM 2020 Journal


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Invisiblemurderlabz
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Registered: 05/18/19
Posts: 550
Loc: The Multiverse
Re: Recognizing and dealing with contamination [Re: nevereataamanita]
    #26637458 - 04/29/20 06:57 PM (3 years, 8 months ago)

Spawned Wheat berries waaaay too early to Coir. Bacteria owned it.
Metabolites(bottom of the shoe-box) fighting like hell. To the garden it went!



Edited by murderlabz (04/29/20 07:05 PM)


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OfflineStargirl
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26639137 - 04/30/20 02:13 PM (3 years, 8 months ago)

Hi,

I've noticed a small green spot in my substrate. A good 1/3 of the jar has already colonized with what looks to be healthy mycelium. I'm guessing it's mold, but I'd appreciate some confirmation! Thanks! (sorry if the picture quality isn't great)



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Offlineoculodextro
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Re: Recognizing and dealing with contamination [Re: Stargirl]
    #26639142 - 04/30/20 02:15 PM (3 years, 8 months ago)

Not looking so great, most likely a contam:(

Kinda looks funky off to the right hand side of the jar too. But hard to tell.


Edited by oculodextro (04/30/20 02:16 PM)


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OfflineCrackatoa
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Re: Recognizing and dealing with contamination [Re: oculodextro]
    #26639441 - 04/30/20 04:38 PM (3 years, 8 months ago)

Trash, sadly.


--------------------

Crack's Tek's and Shit



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OfflineMycena interrupta
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Re: Recognizing and dealing with contamination [Re: Crackatoa]
    #26639795 - 04/30/20 07:24 PM (3 years, 8 months ago)

Could this be cobweb on my GT monotub?
I don't think it is, but someone said it was, so I thought I'd ask here. From what I read in the contams thread, cobweb doesn't seem to appear before pinning.
Thanks for helping a noob with their first grow.

I can't figure out how to add pictures, so here's a link


Edited by Mycena interrupta (04/30/20 07:29 PM)


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Offlineoculodextro
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Re: Recognizing and dealing with contamination [Re: Mycena interrupta]
    #26639839 - 04/30/20 07:59 PM (3 years, 8 months ago)

Hard to tell, I'm not as experienced as the other guys. Just looks like wet myc to me.


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OfflineMycena interrupta
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Re: Recognizing and dealing with contamination [Re: oculodextro]
    #26639922 - 04/30/20 08:39 PM (3 years, 8 months ago)

That's what I was thinking too. It's always looked super healthy, I keep my RH on point, and I only mist to keep surface conditions nice. I even wear an n95 face mask and gloves when I have to open the tub.


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Offlineoculodextro
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Re: Recognizing and dealing with contamination [Re: Mycena interrupta]
    #26639931 - 04/30/20 08:45 PM (3 years, 8 months ago)

Quote:

Mycena interrupta said:
That's what I was thinking too. It's always looked super healthy, I keep my RH on point, and I only mist to keep surface conditions nice. I even wear an n95 face mask and gloves when I have to open the tub.




I learned the hard way that an n95 with a vent, lets air out, but not in.


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OfflineMycena interrupta
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Re: Recognizing and dealing with contamination [Re: oculodextro]
    #26639958 - 04/30/20 09:04 PM (3 years, 8 months ago)

Mine don't have a valve, so it works both ways.


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OfflineCrackatoa
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Re: Recognizing and dealing with contamination [Re: Mycena interrupta]
    #26640007 - 04/30/20 09:41 PM (3 years, 8 months ago)

Quote:

Mycena interrupta said:
Could this be cobweb on my GT monotub?
I don't think it is, but someone said it was, so I thought I'd ask here. From what I read in the contams thread, cobweb doesn't seem to appear before pinning.
Thanks for helping a noob with their first grow.

I can't figure out how to add pictures, so here's a link




You're good. Nothing looks suspicious


--------------------

Crack's Tek's and Shit



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OfflineMycena interrupta
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Re: Recognizing and dealing with contamination [Re: Crackatoa]
    #26641496 - 05/01/20 02:51 PM (3 years, 8 months ago)

Quote:

Crackatoa said:

You're good. Nothing looks suspicious




Awesome, thanks!


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OfflineCelestialexplorer1
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Re: Recognizing and dealing with contamination [Re: Mycena interrupta]
    #26641811 - 05/01/20 05:33 PM (3 years, 8 months ago)

I have a question about two curious agar plates I have. The first is a tropicalis plate and the second is panaeolus cyan Jamaica.

Panaeolus Tropicalis spore plate 4/21/20(plate in question)


Tropicalis Here is a healthy plate as well made on the same day(healthy)


Panaeolus Cyanescens Jamaican spore plate 4/29/20(second plate in question)

Just wondering if anyone has experienced strange growth like this or maybe contamination like this.


--------------------


To spend just one moment in eternity


Edited by Celestialexplorer1 (05/01/20 07:18 PM)


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Offlineucantplaycorsairs
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Re: Recognizing and dealing with contamination [Re: Celestialexplorer1]
    #26642948 - 05/02/20 06:40 AM (3 years, 8 months ago)

I have jar ~30% colonized. Mycelium spot was white and looked nice. I decided to shake the jar and discovered that on the other side rice became blue.
Could you please take a look at the picture, is it spoiled?



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Offlinepoisoned
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Re: Recognizing and dealing with contamination [Re: ucantplaycorsairs]
    #26642956 - 05/02/20 06:44 AM (3 years, 8 months ago)

You need to shake it enough to break up the myc and mix it all up. Best to bang it against a bicycle tire or something. Otherwise, this looks like bruising to me and shouldn't be an issue.


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Offlineoculodextro
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Re: Recognizing and dealing with contamination [Re: poisoned]
    #26642970 - 05/02/20 06:53 AM (3 years, 8 months ago)

Usually when you shake it enough you won't see much mycellium at all, then in a few days the jar should be dense.


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Offlineucantplaycorsairs
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Re: Recognizing and dealing with contamination [Re: oculodextro]
    #26642980 - 05/02/20 06:57 AM (3 years, 8 months ago)

So you want to tell that it's OK that rice is blue and looks rotten?


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