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Mich
Friendly Stranger



Registered: 08/17/22
Posts: 67
Loc: MI
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Re: How to avoid mold in our shoeboxes? [Re: BobRotten]
#27911334 - 08/20/22 01:34 PM (1 year, 5 months ago) |
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I think that or use a number of small containers and just a few drops each to minimize amt of contamination. I think I'll try that next, I have a small PC to process half pint jars.
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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: How to avoid mold in our shoeboxes? [Re: BobRotten]
#27911355 - 08/20/22 01:47 PM (1 year, 5 months ago) |
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Quote:
BobRotten said: People are advising against spore syringes, but what do you do if you can't get LC? put the spore syringe on some agar and use the clean bit in with the spawn?
PF tek is the best method to use a MSS without agar work. Otherwise cleaning on agar is necessary.
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: How to avoid mold in our shoeboxes? [Re: Smellyhobbit]
#27911474 - 08/20/22 03:28 PM (1 year, 5 months ago) |
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Once a monotub gets trich and you toss out your sub, do you reuse the monotub or trash that as well? How do you clean it to be sure your next run won't get trich from it again?
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Smellyhobbit
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Re: How to avoid mold in our shoeboxes? [Re: Bigworm] 1
#27911483 - 08/20/22 03:35 PM (1 year, 5 months ago) |
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Quote:
Bigworm said: Once a monotub gets trich and you toss out your sub, do you reuse the monotub or trash that as well? How do you clean it to be sure your next run won't get trich from it again?
I just wash mine out, run them through the dish washer, and use them again. But that’s shoeboxes. A bigger tub, I’d wash it out and then let it dry in the air for a few days. But I don’t use bugger tubs so I might be wrong
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B Traven
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Re: How to avoid mold in our shoeboxes? [Re: Bigworm] 3
#27911763 - 08/20/22 07:32 PM (1 year, 5 months ago) |
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Quote:
Bigworm said: Once a monotub gets trich and you toss out your sub, do you reuse the monotub or trash that as well? How do you clean it to be sure your next run won't get trich from it again?
No, we don't throw contaminated containers away- jars, tubs, what-have-you. Just toss the material and clean the vessel.
Remember that trich is just the final blow. 9 times out of 10, it's something else that led to the myc being too weak to fight it off. Trichoderma is also basically everywhere in our soil, so there are probably always trich spores floating around. We're really just trying to keep the spore load within reasonable parameters, whether we realize it or not.
I'm paranoid, and also run shoeboxes which fit easily in the dishwasher. So I dump the sub, rinse the tub, then run it through the dishwasher. Right before using a tub again, when it's coming back out of the storage stack, I pour some boiling water into it and let it sit. I can nest about a dozen shoeboxes inside each other, filling each with boiling water, while they all sit inside a large tub that catches the spillover. I let them sit like that until the water is cooled down to a temperature that won't burn my skin, dump, and then proceed.
I like overkill when it comes to wiping the slate clean.
-------------------- Beware of advice- even this.
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: How to avoid mold in our shoeboxes? [Re: B Traven]
#27911779 - 08/20/22 07:46 PM (1 year, 5 months ago) |
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Ok so I guess if I wash it then fill it with bleach water and let it sit for a few hours I'll be good too? I don't want to waste plastic so I'm happy I can reuse them.
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B Traven
Stranger



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Re: How to avoid mold in our shoeboxes? [Re: Bigworm] 1
#27911797 - 08/20/22 08:01 PM (1 year, 5 months ago) |
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Quote:
Bigworm said: Ok so I guess if I wash it then fill it with bleach water and let it sit for a few hours I'll be good too? I don't want to waste plastic so I'm happy I can reuse them.
Yeah, should be.
Actually, I think that simply rinsing with water is the most effective way to get rid of contaminant spores. Chemicals kill them to some degree and high heat is the only thing that's completely effective at killing them (why we PC grain), but water does a great job of simply washing them down the drain.
I used to be more heavy-handed with the bleach and lysol and whatnot. Now the only chemicals I'm down to are 70% isopropyl for sanitizing tools and containers before transfers, and everclear for my ghetto alcohol lamp setup, for flaming my scalpel.
-------------------- Beware of advice- even this.
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PBJ710
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Re: How to avoid mold in our shoeboxes? [Re: Bigworm] 1
#27912050 - 08/21/22 04:55 AM (1 year, 5 months ago) |
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Quote:
Bigworm said: Ok so I guess if I wash it then fill it with bleach water and let it sit for a few hours I'll be good too? I don't want to waste plastic so I'm happy I can reuse them.
Don't even waste the bleach - just rinse out any physcial debris before cleaning the tub with soap/water.
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BobRotten
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Registered: 08/10/22
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Re: How to avoid mold in our shoeboxes? [Re: Smellyhobbit]
#27912088 - 08/21/22 06:22 AM (1 year, 5 months ago) |
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Quote:
Smellyhobbit said:
Quote:
BobRotten said: People are advising against spore syringes, but what do you do if you can't get LC? put the spore syringe on some agar and use the clean bit in with the spawn?
PF tek is the best method to use a MSS without agar work. Otherwise cleaning on agar is necessary.
What is MSS?
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Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
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Re: How to avoid mold in our shoeboxes? [Re: BobRotten]
#27912092 - 08/21/22 06:32 AM (1 year, 5 months ago) |
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Quote:
BobRotten said:
Quote:
Smellyhobbit said:
Quote:
BobRotten said: People are advising against spore syringes, but what do you do if you can't get LC? put the spore syringe on some agar and use the clean bit in with the spawn?
PF tek is the best method to use a MSS without agar work. Otherwise cleaning on agar is necessary.
What is MSS?
Multi-spore syringe
-------------------- Being pissed on does not make you a real man.
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BobRotten
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Re: How to avoid mold in our shoeboxes? [Re: Guerrilla]
#27912097 - 08/21/22 06:35 AM (1 year, 5 months ago) |
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cheers
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Mich
Friendly Stranger



Registered: 08/17/22
Posts: 67
Loc: MI
Last seen: 1 month, 26 days
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Re: Recognizing and dealing with contamination [Re: Mich]
#27912296 - 08/21/22 10:46 AM (1 year, 5 months ago) |
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Party's over!
[/url]]https:// [/url]
First obvious contamination showed up. Wonder how long till it takes over?? [url= ]
Edited by Mich (08/21/22 05:41 PM)
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metsamen
Stranger

Registered: 08/21/22
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Re: Recognizing and dealing with contamination [Re: Mich]
#27913086 - 08/22/22 02:28 AM (1 year, 5 months ago) |
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Hello guys, I'm new to this and before I found this forum I started uncle bens tek. So I've got 7 bags of brown rice. 2 was contaminated and 5 went good. I mixed it with 1:3 ratio to coir and put in 2 small boxes and into monotub (unmodified). After 7-10 days it was 80% colonized so I flipped the lid left a little gap and added LED light. I'm keeping temperature around 22C and humidity around 75-85%. 10 days after boxes look a bit different and I'm wondering if it is contamination or not? Especially the right box, phone makes it look white, but I can see a bit blue or green.
is
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BobRotten
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Re: Recognizing and dealing with contamination [Re: metsamen]
#27913251 - 08/22/22 07:20 AM (1 year, 5 months ago) |
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 I've been told this is bacterial mycelium, I hadn't heard of that before this forum, If I put it to bulk what is likely to happen?
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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: Recognizing and dealing with contamination [Re: BobRotten]
#27913256 - 08/22/22 07:23 AM (1 year, 5 months ago) |
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Quote:
BobRotten said:
 I've been told this is bacterial mycelium, I hadn't heard of that before this forum, If I put it to bulk what is likely to happen?
If you shred this to bulk, it’s likely that it won’t recover and a competing organism will take over. Probably trichoderma.
Best practice is to fruit it as is.
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BobRotten
Stranger
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#27913277 - 08/22/22 07:47 AM (1 year, 5 months ago) |
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Cheers, Do you know much about Oysters, I swear I saw something similar in one jar then the Oyster took over. And am worried I used similar jars to bulk before I knew what it was, though they seem to look fine so far, though haven't spawned.
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Mich
Friendly Stranger



Registered: 08/17/22
Posts: 67
Loc: MI
Last seen: 1 month, 26 days
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Re: Recognizing and dealing with contamination [Re: BobRotten]
#27913620 - 08/22/22 12:33 PM (1 year, 5 months ago) |
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It almost looks like a crack in the jar?
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cozmyc
gentle modern ape



Registered: 06/20/21
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Re: Recognizing and dealing with contamination [Re: Mich] 3
#27914944 - 08/23/22 10:11 AM (1 year, 5 months ago) |
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Death by Demogorgon.
-------------------- You're conscious population 2 stardust ---------------------- and that's valuable
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Mich
Friendly Stranger



Registered: 08/17/22
Posts: 67
Loc: MI
Last seen: 1 month, 26 days
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Re: Recognizing and dealing with contamination [Re: cozmyc]
#27915253 - 08/23/22 01:53 PM (1 year, 5 months ago) |
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I think I'll just dig a shallow grave for my fallen grains, there is a sprinkle of blue-green crusty stuff on some grains.
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Rnb1987
Newb

Registered: 10/16/19
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Re: Recognizing and dealing with contamination [Re: Mich]
#27916287 - 08/24/22 09:36 AM (1 year, 5 months ago) |
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I am not sure where to post this anymore... Do I start a new thread or post here... I apologize to the mods if this is on the wrong place.
I have some jars of oats and I’m not sure what exactly I’m looking at. At first I thought it was myc because I just shook the jars a little two days ago. Not so sure anymore... Can anyone tell me what I’m looking at here? Thank you
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