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Smellyhobbit
Actual Retard



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Re: Recognizing and dealing with contamination [Re: Kizzle]
#27899474 - 08/11/22 07:08 PM (1 year, 5 months ago) |
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Isn’t that what you do with contaminated jars? Or we could eat it.
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Rotnpins
🤮 Rotten-Pins 🍄



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Re: Recognizing and dealing with contamination [Re: Bigworm] 1
#27899625 - 08/11/22 08:28 PM (1 year, 5 months ago) |
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Quote:
Bigworm said:
Quote:
ObakeRocky said: Just to explain, I have these shoeboxes that got weird, I think because my dumb ass forgot to put a light above them on the high closet shelf where they live. I just had ambient window light and a lamp across the room. They colonized for like 2 weeks like that until they started pinning. But I got all these side/bottom pins. So I put up a light and I tried flipping the whole substrate and they’ve responded pretty well.
Only concern now is what seems to be aerial mycelium but I’m not positive. I’ve had a hard time finding pics of the same growth up the stipes. I harvested a handful already, folded ‘em in a paper towel and popped ‘em in the fridge while I decided what to do. When I pulled them out the white fluff was completely flat. https:// https://
Please if anyone can educate me, I would be grateful
I haven't seen this type of myc in my short time doing this but I could make a guess. IMO it looks like it's dry and starving for FAE. Please don't take what I say as being mean. I say dry cause the fruits look like mine do after being in my dehydrator for a couple hours and you have yellow mycelium. The fuzz growing on your fruits are a sign of low FAE or some type of contamination I have not had experience with but I would lean to lack of FAE. Although it's not on all of them. So maybe it's not a FAE problem. I think you could try misting a bit and see how long it takes to soak in. I'm sure someone with more knowledge can help you out better than me.
I'd recommend checking out the "surface conditions" link in my signature.. it looks dry..
Also, wats with the big crater in the center of the tub? Did you use another tub or something to compress your substrate?
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
#27900263 - 08/12/22 08:28 AM (1 year, 5 months ago) |
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Quote:
Rotnpins said:
Quote:
Bigworm said:
Quote:
ObakeRocky said: Just to explain, I have these shoeboxes that got weird, I think because my dumb ass forgot to put a light above them on the high closet shelf where they live. I just had ambient window light and a lamp across the room. They colonized for like 2 weeks like that until they started pinning. But I got all these side/bottom pins. So I put up a light and I tried flipping the whole substrate and they’ve responded pretty well.
Only concern now is what seems to be aerial mycelium but I’m not positive. I’ve had a hard time finding pics of the same growth up the stipes. I harvested a handful already, folded ‘em in a paper towel and popped ‘em in the fridge while I decided what to do. When I pulled them out the white fluff was completely flat. https:// https://
Please if anyone can educate me, I would be grateful
I haven't seen this type of myc in my short time doing this but I could make a guess. IMO it looks like it's dry and starving for FAE. Please don't take what I say as being mean. I say dry cause the fruits look like mine do after being in my dehydrator for a couple hours and you have yellow mycelium. The fuzz growing on your fruits are a sign of low FAE or some type of contamination I have not had experience with but I would lean to lack of FAE. Although it's not on all of them. So maybe it's not a FAE problem. I think you could try misting a bit and see how long it takes to soak in. I'm sure someone with more knowledge can help you out better than me.
I'd recommend checking out the "surface conditions" link in my signature.. it looks dry..
Also, wats with the big crater in the center of the tub? Did you use another tub or something to compress your substrate?
I think if I read it right, they flipped the substrate over. It's probably the dent from the bottom of the tub originally on the underside.
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Hindsight
Mad Scientist


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Re: Recognizing and dealing with contamination [Re: SilverSumm]
#27900272 - 08/12/22 08:34 AM (1 year, 5 months ago) |
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Quote:
albertor said: Hello, I'm new bear with me. My jar was colonized on all sides but the bottom. This morning I recognized this ugly looking slime. There's no salvaging this by scooping the top, correct? I'm going to put this in a trash bag within another trash bag for now.

Yes that is bacterial. Really badly too. I would toss it.
Quote:
SilverSumm said: This is pan cyan myc. I’m suspecting it is bruising but can anyone confirm?

The blue is bruising, most likely due to bacterial infection. Note the excess moisture on the sides of the jar, which is another sign, as well as how thick that mycelium is. Pan mycelium is generally much thinner than that - it does vary a bit from one variety to the next but while bacterial cube mycelium looks solid white like marshmallow paste, bacterial pan mycelium looks a bit less dense, but much more dense than healthy pan mycelium. Due to wide variation in pan mycelium density, density alone isn't usually good enough to conclusively identify contamination, but add some bruising and a bunch of condensation and it's a sure bet.
Here is what a healthy jar of pan mycelium should look like:
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camob
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Re: Recognizing and dealing with contamination [Re: Hindsight]
#27903116 - 08/14/22 12:10 PM (1 year, 5 months ago) |
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Hey all, first post! Got my first kit then found this amazing site, promptly made a sgfc and transferred just as pinning started. 260g wet first flush, very happy.
Does the below pic show contam? The growth seems a little different from the orig mycelium and I know some contam can look similar.
Basically I harvested, and scraped the vermy base off the stems as I went, dumping it back on top of the cake, making the slight mound you see. Went away for 2 days and left the cake like that with lid on to 'regenerate' which I've read about, planning to re-dunk tonight for second flush. This is what I've found coming back. Is this the good mycelium re-claiming territory or something else?
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#27903120 - 08/14/22 12:16 PM (1 year, 5 months ago) |
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That looks like mycelium to me.
Next time after harvest I recommend dunking first, then scraping aborts/loose verm/junk off, then putting fresh verm or coir on top.
Since it's already started recovering, I'd suggest you heavily mist it for a few days instead of dunking. I think you'll have better luck that way.
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camob
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#27903367 - 08/14/22 04:05 PM (1 year, 5 months ago) |
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Cheers, I'll give that a go
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foxdl99
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Re: Recognizing and dealing with contamination [Re: camob]
#27906519 - 08/17/22 08:59 AM (1 year, 5 months ago) |
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Brand new here, second inoculation of grain spawn bag. I've been sifting through pictures trying to find something similar and come up empty. Is this just the grain color poking through? Contamination? I tried to monotub a previous bag and had super fluffy white stuff that never fruited and eventually turned green. If I'm doing something wrong I'd love to know what it is, took most every sanitization precaution I could think of. I have a room under some stairs with no air flow, wore a mask, washed hands, etc. I'd hate to waste any more time.
Edited by foxdl99 (08/17/22 09:18 AM)
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: foxdl99]
#27906542 - 08/17/22 09:17 AM (1 year, 5 months ago) |
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Quote:
foxdl99 said: Brand new here, second inoculation of grain spawn bag. I've been sifting through pictures trying to find something similar and come up empty. Is this just the grain color poking through? Contamination? I tried to monotub a previous bag and had super fluffy white stuff that never fruited and eventually turned green. If I'm doing something wrong I'd love to know what it is, took most every sanitization precaution I could think of. I have a room under some stairs with no air flow, wore a mask, washed hands, etc. I'd hate to waste any more time.




It's the grain touching the bag. They are bacterial though. I see metabolite, excess moisture, the red brown color on the bottom, and the grain touching the bag are all not good signs. If you started with a syringe straight to grain, that's where you went wrong. Try a pf tek grow first or start out on agar.
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monkey_accident
fail now learn later


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Re: Recognizing and dealing with contamination [Re: Bigworm]
#27906548 - 08/17/22 09:19 AM (1 year, 5 months ago) |
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i would top fruit that
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: monkey_accident]
#27906553 - 08/17/22 09:21 AM (1 year, 5 months ago) |
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Quote:
monkey_accident said:

i would top fruit that
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foxdl99
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Re: Recognizing and dealing with contamination [Re: Bigworm]
#27906558 - 08/17/22 09:26 AM (1 year, 5 months ago) |
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Lame. Top fruiting is just cutting the top off the bag I guess and letting it fruit?
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monkey_accident
fail now learn later


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Re: Recognizing and dealing with contamination [Re: foxdl99]
#27906559 - 08/17/22 09:27 AM (1 year, 5 months ago) |
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youll need to add some cv on top as well but yes
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: foxdl99]
#27906628 - 08/17/22 11:05 AM (1 year, 5 months ago) |
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The reason you top fruit is because your mycelium is contaminated and if you break it up to put into a monotub or whatever it might not recover. At least with top fruiting you stand a better chance to get something out of it. Whatever does grow I suggest you grab a clone and some prints and try again. If you are very steril in your work you shouldn't have an issue on the next try with a clean clone on agar.
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Rotnpins
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Re: Recognizing and dealing with contamination [Re: foxdl99]
#27906649 - 08/17/22 11:26 AM (1 year, 5 months ago) |
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Quote:
foxdl99 said: Brand new here, second inoculation of grain spawn bag. I've been sifting through pictures trying to find something similar and come up empty. Is this just the grain color poking through? Contamination? I tried to monotub a previous bag and had super fluffy white stuff that never fruited and eventually turned green. If I'm doing something wrong I'd love to know what it is, took most every sanitization precaution I could think of. I have a room under some stairs with no air flow, wore a mask, washed hands, etc. I'd hate to waste any more time.

How did you inoculate the bag? Check out the link in my signature that says "every noob should read this".. I have a feeling it will answer your question
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Averroes
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
#27906842 - 08/17/22 02:22 PM (1 year, 5 months ago) |
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I have two nodule-like concretions. When I touch it with a paper towel, there is a clay like bluish-green residue that crumbles. This is my third slab in a mono and I have had moderately good success. Two days ago I put H2O2 and salt on the two spots and isolated the tub.
PES Amazoninia. Rye spawn. This batch was not from agar, but straight from multi-spore syringe. Nutritious substrate (horse poo) from shroomery vendor, which I have had good success with.
I have looked at dozens and dozens of photos, but don't see anything quite like this. My first thought was trich; my second thought was sclerotia. But honestly, I don't know.
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foxdl99
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
#27907144 - 08/17/22 06:04 PM (1 year, 5 months ago) |
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Thanks man, I did actually start PF Tek recently. I didn't use a SAB though, used oven door method. I'll look into AGAR, I've seen a few videos on pouring my own so maybe I head down that road. Given my lack of success thus far using syringe I guess I'll see how PF goes going forward. Thanks again!
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Bigworm



Registered: 03/19/22
Posts: 1,868
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Re: Recognizing and dealing with contamination [Re: foxdl99] 1
#27907166 - 08/17/22 06:27 PM (1 year, 5 months ago) |
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Quote:
foxdl99 said: Thanks man, I did actually start PF Tek recently. I didn't use a SAB though, used oven door method. I'll look into AGAR, I've seen a few videos on pouring my own so maybe I head down that road. Given my lack of success thus far using syringe I guess I'll see how PF goes going forward. Thanks again!
Just look at it as you know what not to do now. You never really fail in this hobby, you just learn. I've had a bunch of fails but they make the successful runs that much more enjoyable. Hopefully you top fruit that and get something good from it. It definitely has the potential to give you some nice fruit.
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outlier52
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Registered: 02/17/22
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Re: Recognizing and dealing with contamination [Re: Bigworm]
#27907662 - 08/18/22 04:33 AM (1 year, 5 months ago) |
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Bag O’ APE, looking contaminated AF after 41 days. I think the blue-green is from handling it too much- my noobian fuckery. It was in a similar state of colonization 14 days ago, and I broke the bag up as sort of a “make it or break it” Hail Mary. Remarkably, it recovered and was back at it the next day.
I’d like to get something out of this if I can. My plans were for a P9 shoebox, but no way this spawn would survive his ratio of something like 1:5. I could always fruit from the bag. I’m giving it another week and it’s going somewhere, hopefully in a shoebox at 1:2 minus the P9 layer, blobs be damned.


Edited by outlier52 (08/18/22 04:36 AM)
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BobRotten
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Re: Recognizing and dealing with contamination [Re: outlier52]
#27907733 - 08/18/22 06:29 AM (1 year, 5 months ago) |
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 So I think this is overlay, what do others think, it's just oyster which Is crazy virulent mycelium, all this is after like 5 days. Sorry for shitty pic, I don't wanna open it yet as it's so young.
Edited by BobRotten (08/18/22 06:32 AM)
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