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Klh56523
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Registered: 06/16/22
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#27869296 - 07/19/22 05:21 PM (1 year, 6 months ago) |
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Hi,
This is my first time trying to grow mycelium from different spore swab and print. Initially I tried to inoculate substrate with spore syringes and they didn't work because of contam and plus I couldn't tell if the white stuff was mycelium. I thought growing my own in agar might be easier but so far I don't know they look too fuzzy. The first pic is from a spore print where I scrapped a bunch of spores onto the agar. The little dots are condensation. The last 2 are from a spore swab.


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0120


Registered: 12/13/21
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Re: Recognizing and dealing with contamination [Re: Klh56523]
#27869976 - 07/20/22 07:31 AM (1 year, 6 months ago) |
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Whats that dots? It’s freshly poured agar all plates look same
Edited by 0120 (07/20/22 07:47 AM)
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PBJ710
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Re: Recognizing and dealing with contamination [Re: 0120]
#27870027 - 07/20/22 08:30 AM (1 year, 6 months ago) |
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Looks like alot of sediment that doesn't dissolve in water. What did you use for your agar recipe?
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0120


Registered: 12/13/21
Posts: 44
Last seen: 1 year, 3 months
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Re: Recognizing and dealing with contamination [Re: 0120]
#27870092 - 07/20/22 09:35 AM (1 year, 6 months ago) |
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10lme10agar0.5hotwater. it kinda had problem with dissolving (i was mixing like 5 min and mixed pretty well) it had some heavier mass bottom and watery layer top not somuch but there was 2 layers when i pc-ed it was looking good and even liquid.
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PBJ710
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Re: Recognizing and dealing with contamination [Re: 0120]
#27870145 - 07/20/22 10:25 AM (1 year, 6 months ago) |
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Not sure what it is, but it shouldn't be there with that recipe unless one of your ingredients was dirty. Both plates appear to have bacterial growth going on.
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Sill
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Registered: 06/30/22
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Re: Recognizing and dealing with contamination [Re: PBJ710]
#27871131 - 07/21/22 03:21 AM (1 year, 6 months ago) |
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Hi guys 👋🏼
Mycelium growth in shoe box has almost stalled the last couple of days, now it’s been three weeks since spawning fully colonized rye. I’ve been fanning and misting daily in the last three days. Ratio 1:3
This is how the show box looks now
Photo right after misting

Questions:
- Any signs of contam? - Am I misting too much?
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#27871136 - 07/21/22 03:37 AM (1 year, 6 months ago) |
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I’m not sure about contam, but don’t fan. Fanning is outdated. Get your surface conditions right and then let passive FAE take care of evaporation.
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Sill
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit] 1
#27871271 - 07/21/22 07:41 AM (1 year, 6 months ago) |
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Thanks Smellyhobbit for your response..
Any idea what might be causing the slow down in mycelium growth?
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PBJ710
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Re: Recognizing and dealing with contamination [Re: Sill]
#27871284 - 07/21/22 08:12 AM (1 year, 6 months ago) |
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Quote:
Sill said: Thanks Smellyhobbit for your response..
Any idea what might be causing the slow down in mycelium growth?
Fanning and misting which is damaging the surface conditions. Setup your passive FAE correctly and stop manually interfering with their growth. If you are needing to mist more than once or twice at most before first harvest, you have too much FAE.
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Sill
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Re: Recognizing and dealing with contamination [Re: PBJ710]
#27871526 - 07/21/22 12:35 PM (1 year, 6 months ago) |
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Thanks PBJ710. Very helpful
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maimunji
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Re: Recognizing and dealing with contamination [Re: Sill]
#27877519 - 07/26/22 07:12 AM (1 year, 6 months ago) |
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I spawn this 3 jars to coir in separate shoeboxes and all 3 start to show green before full colonization. Do they look really that bad?



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Alex444
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Registered: 07/26/22
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Re: Recognizing and dealing with contamination [Re: maimunji]
#27877544 - 07/26/22 07:38 AM (1 year, 6 months ago) |
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Does this look good? First when I got the growkit it smelled sour...where I bought the Kit they say that the rye is the reason it smelled a bit sour...now there is no sour smell left...only mushroom smell Hope someone can help🙏
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: maimunji]
#27877649 - 07/26/22 09:18 AM (1 year, 5 months ago) |
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Quote:
maimunji said: I spawn this 3 jars to coir in separate shoeboxes and all 3 start to show green before full colonization. Do they look really that bad?




If your shoeboxes are showing contamination why show the jars? Jars look ok to me.
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: Alex444]
#27877650 - 07/26/22 09:20 AM (1 year, 5 months ago) |
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Quote:
Alex444 said:

Does this look good? First when I got the growkit it smelled sour...where I bought the Kit they say that the rye is the reason it smelled a bit sour...now there is no sour smell left...only mushroom smell Hope someone can help🙏
Sour smells aren't good. I never had rye smell sour but that doesn't mean it couldn't. The tubs look ok to my untrained eyes. I don't see metabolites or any off colors so if say let it ride.
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Rotnpins
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Re: Recognizing and dealing with contamination [Re: Bigworm] 1
#27877670 - 07/26/22 09:38 AM (1 year, 5 months ago) |
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Quote:
Bigworm said:
Quote:
maimunji said: I spawn this 3 jars to coir in separate shoeboxes and all 3 start to show green before full colonization. Do they look really that bad?




If your shoeboxes are showing contamination why show the jars? Jars look ok to me.
Because the spawn is the most likely source of contamination.
if posting the tubs, the response would probably be "yup, those are contaminated, what did your spawn look like"
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Rotnpins
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Re: Recognizing and dealing with contamination [Re: Alex444]
#27877674 - 07/26/22 09:40 AM (1 year, 5 months ago) |
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Quote:
Alex444 said:

Does this look good? First when I got the growkit it smelled sour...where I bought the Kit they say that the rye is the reason it smelled a bit sour...now there is no sour smell left...only mushroom smell Hope someone can help🙏
Are those tubs just straight grain? No substrate?
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
#27877692 - 07/26/22 09:50 AM (1 year, 5 months ago) |
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Quote:
Rotnpins said:
Quote:
Bigworm said:
Quote:
maimunji said: I spawn this 3 jars to coir in separate shoeboxes and all 3 start to show green before full colonization. Do they look really that bad?




If your shoeboxes are showing contamination why show the jars? Jars look ok to me.
Because the spawn is the most likely source of contamination.
if posting the tubs, the response would probably be "yup, those are contaminated, what did your spawn look like"
Ahh, I see what you were getting at now. My mistake. I thought you were asking if the bins looked bad. I only see a little extra moisture in there and it's minimal.
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Alex444
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
#27877774 - 07/26/22 10:59 AM (1 year, 5 months ago) |
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Not just grain...its a substrate...its just that the vendor told me that the grain has naturaly a sour smell...don't know if he lies to me...I will observe it over the next days if there is a color change...for now its white
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#27877780 - 07/26/22 11:03 AM (1 year, 5 months ago) |
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Look for droplets of amber liquid. Those are metabolites from the mycelium fighting infection. A little is fine, but a lot usually indicates infection. Or of course competing fungus overtaking infected and weakened mycelium.
Either way, good luck!
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Rotnpins
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#27878179 - 07/26/22 04:37 PM (1 year, 5 months ago) |
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So, I've been struggling with this Tidal Wave clone culture for a while.. I think it's enmeshed with something that I can't seem to get rid of.. it gets to right around the first flush pins and trichs out on me... I've taken plate pins to new plates and had the plate pin go south on me..
This jar looks exactly like the last 2 that contaminated right before the first flush.. does it have some visual signs that are indicators of the spawn being the issue?
I'm thinking about trying to top fruit this one to see if I can actually get something.. (I know, it's probably time to trash the culture and move on)

I'm taking one last stab at it after this jar with a plate pin I cut open and took a tissue sample of to see if that will come out clean..
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