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Evolvedr79
Stranger
Registered: 04/21/19
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Last seen: 3 years, 4 days
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Re: Recognizing and dealing with contamination [Re: poisoned]
#26610360 - 04/18/20 12:13 PM (3 years, 9 months ago) |
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Hello everyone. This tub is one of three that came from a WBS spawn. Substrate is CVG. The other two tubs produced 2500 wet grams with a 12.5:1 wet to dry ratio. This last tub looks to have metabolites to me which would indicate a contamination but I am not sure which it is. I've only ever dealt with Trich in my previous tubs. The yellow coloring is abnormal to me.
Can someone please tell me if this tub is contaminated and if so, what is it?
Thanks.

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DezGWest7
JacQ


Registered: 04/09/20
Posts: 29
Last seen: 1 year, 8 months
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Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
#26610619 - 04/18/20 01:52 PM (3 years, 9 months ago) |
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Quote:
DezGWest7 said: What's up guys. First timer here. I inoculated WBS March 27 in 3 Jars with GT. The thing is, i see NO activity in 2 of them and the one that I do see activity, I believe its probably contaminated but I'm not so certain. Here is a pic of it. I left it alone originally for the 1st 9 days or so until i decided to check it out and saw what the picture shows but smaller and yes, growth in those 2 different spots. I'm not sure where I f'ed up but *note- this WBS preparation did not include coffee grounds or gypsum. I rinsed , picked the floating grain off, rinsed until clear - soaked 20+ hours - simmered stovetop - then strained , spread out and left to dry a little over 2 hours(I recall it wasn't 100% dry when I decided to pack in the Jar for PS 120min Sterilization. Was it wet wet ? Noo. It just had enough moisture for me to notice when the grains were touching the inside of the jar after loading up. After the PC I allowed it to cool down more than 16 hours from my recollection & then I did the whole sterilize GT syringe etc inoculation of jars.




Feedback is appreciated.
Since no one gave me any feedback I waited to see how it would progress and I suppose it answered any questions I had. This is how it looks as of today
Edited by DezGWest7 (04/18/20 01:53 PM)
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WillieFunGuy
Stranger

Registered: 02/11/20
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Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
#26622479 - 04/23/20 02:12 PM (3 years, 9 months ago) |
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Dude...looks perfect to me. I understand your trepidation though. I’m fairly new myself. Contams happen to the best of us. Keep it moving. Good job!
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
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Re: [STICKY] Recognizing and dealing with contamination [Re: WillieFunGuy] 1
#26622501 - 04/23/20 02:18 PM (3 years, 9 months ago) |
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Give her a shake 👍
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 5 days
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Re: [STICKY] Recognizing and dealing with contamination [Re: Crackatoa]
#26623669 - 04/23/20 11:47 PM (3 years, 9 months ago) |
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Is this some stage of knots/primordia I've never noticed before or am I growing something freaky?
-------------------- My LAGM 2020 Journal
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murderlabz
RIP Stoneman


Registered: 05/18/19
Posts: 550
Loc: The Multiverse
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Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita]
#26623681 - 04/23/20 11:56 PM (3 years, 9 months ago) |
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Quote:
nevereataamanita said: Is this some stage of knots/primordia I've never noticed before or am I growing something freaky?

Looks like it's building up Metabolites getting ready for war with something.
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 5 days
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Re: [STICKY] Recognizing and dealing with contamination [Re: murderlabz]
#26623689 - 04/24/20 12:03 AM (3 years, 9 months ago) |
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Damn, guess I'll keep an eye out and be ready to toss the shoebox. Here's a pic of the whole surface, I haven't spotted the contam yet.
-------------------- My LAGM 2020 Journal
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poisoned
untitled



Registered: 04/17/13
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Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita]
#26624749 - 04/24/20 01:15 PM (3 years, 8 months ago) |
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Probably a bacteria, you won't really see it and you'll still get fruits.
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 5 days
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Re: [STICKY] Recognizing and dealing with contamination [Re: poisoned]
#26624761 - 04/24/20 01:19 PM (3 years, 8 months ago) |
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I've been wondering, is it a bad idea to clone or print from a bacterial grow? Is that shit going to get passed on or could you get away from it on agar?
-------------------- My LAGM 2020 Journal
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
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Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita] 1
#26624801 - 04/24/20 01:39 PM (3 years, 8 months ago) |
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usually fruits won't host other organisms on the inside.
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PinkStormtrooper
Jet-Puffed


Registered: 04/11/20
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Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
#26625847 - 04/24/20 10:40 PM (3 years, 8 months ago) |
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once they get 30% shake em like hell then leave em sit until they are 99% then plant those dudes. i cant tell you why its so slow tho. i could only suggest per reading your steps PC for 60min not 120min but looks good keep going.
-------------------- "say, you got a little astroglide on your moustache"
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jeezy1021
Stranger


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Re: [STICKY] Recognizing and dealing with contamination [Re: PinkStormtrooper]
#26628134 - 04/25/20 08:45 PM (3 years, 8 months ago) |
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does this look contaminated
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: [STICKY] Recognizing and dealing with contamination [Re: jeezy1021]
#26628199 - 04/25/20 09:17 PM (3 years, 8 months ago) |
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It doesn't look great but there's one way to find out for sure and that's to fruit it, you can toss it when it becomes too obvious that it's contammed or if it doesn't produce any fruits.
-------------------- Cakes inside Water Tub
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StonedTurtle
Stranger



Registered: 02/22/17
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Re: [STICKY] Recognizing and dealing with contamination [Re: Mateja]
#26628507 - 04/26/20 12:15 AM (3 years, 8 months ago) |
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So there's one area on my jar that looks extremely white and the other jars lack this. The part I find crazy is that the area isn't growing. Theres a small area beside it, that I can tell is mycelium. Im assuming that stopped the growth of the extremely white part? I don't know. I'm not sure if I should be worried,but outside opinions are always good thanks for the help
-------------------- Some of my favorite quotes: "Happy the man who improves other people not merely when he is in their presence but when he is in their thoughts" -Seneca "Intelligence tells you how to rule the world, wisdom tells you how to keep the world" "Any fool can criticize, condemn and complain - and most fools do. But it takes character and self-control to be understanding and forgiving"
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StoneTroegs
Mycopath
Registered: 11/04/18
Posts: 20
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Interesting Contam on Agar [Re: Kizzle]
#26631378 - 04/27/20 04:59 AM (3 years, 8 months ago) |
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Can anyone ID the orange blob contam on my agar dish? It’s pretty cool looking and I’m trying to get more knowledge different growths
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RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
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Re: Interesting Contam on Agar [Re: StoneTroegs]
#26632150 - 04/27/20 01:05 PM (3 years, 8 months ago) |
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Quote:
StoneTroegs said:

Can anyone ID the orange blob contam on my agar dish? It’s pretty cool looking and I’m trying to get more knowledge different growths
The yellow blob is most likely bacteria, the reasons I say this are:
(1) colorful contaminants are generally bacteria
(2) it looks to be on the wet side, which is another indicator of bacterial growth.
your main culture itself, if it's supposed to be GT looks a bit funny too.
It's a little hard for me to tell from the image, but the fact that you have anything other than white myc is a bit of a red flag in my book.
Discoloration can be from metabolites or another fungi that you weren't looking to grow.
The beauty of doing your work on an agar dish is that you may still be able to transfer the best looking portion of the leading edge to a clean dish in order to leave the contamination behind.
That is assuming that your culture is still GT and not something else.
I have not done so myself yet but you can also mix things into the agar to inhibit bacterial growth. I think these include h202 agar and the antibiotic germentacin are examples.
I purchased the latter from one of the sponsors here.
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StoneTroegs
Mycopath
Registered: 11/04/18
Posts: 20
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Re: Interesting Contam on Agar [Re: RyeHumor]
#26632368 - 04/27/20 03:12 PM (3 years, 8 months ago) |
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Thanks for the reply. I liked the look of the blob so I wanted to see if anyone knew the bacteria name. As for the GT I’m calling it a loss. I’ve got some good looking albino a+ and Puerto Rican ready for transfer.
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RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
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Re: Interesting Contam on Agar [Re: StoneTroegs]
#26632416 - 04/27/20 03:32 PM (3 years, 8 months ago) |
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Oh I see. Well it looks like apricot jelly...
wonder what it tastes like 
And come to think of it that myc looks discolored similar to the appearance the foam on a saltwater fish tank protein skimmer looks.
That kind of brown rust orange fluff on the top.
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Kkgal420
Stranger

Registered: 02/02/20
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Is this contamination or new growth? [Re: StonedTurtle]
#26632580 - 04/27/20 05:07 PM (3 years, 8 months ago) |
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I added verm/coir on the tops 9 days ago and I have white stuff growing on the tops. I can't figure out if it's contaminated or new growth from looking at the forums. What do y'all think?
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Mindgmz
Stranger

Registered: 04/28/20
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Is this contamination? [Re: Kkgal420]
#26635180 - 04/28/20 07:28 PM (3 years, 8 months ago) |
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First timer here, I am 3 weeks in and 9 jars almost completely colonized but I have this 1 I can't determine if it is contaminated or the dry verm slid down some. Any help is appreciated. Thanks
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