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OfflineEvolvedr79
Stranger
Registered: 04/21/19
Posts: 4
Last seen: 2 months, 2 days
Re: Recognizing and dealing with contamination [Re: poisoned]
    #26610360 - 04/18/20 12:13 PM (2 months, 21 days ago)

Hello everyone. This tub is one of three that came from a WBS spawn. Substrate is CVG. The other two tubs produced 2500 wet grams with a 12.5:1 wet to dry ratio. This last tub looks to have metabolites to me which would indicate a contamination but I am not sure which it is. I've only ever dealt with Trich in my previous tubs. The yellow coloring is abnormal to me.

Can someone please tell me if this tub is contaminated and if so, what is it?

Thanks.






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OfflineDezGWest7
CheckYourEgoAlways

Registered: 04/09/20
Posts: 26
Last seen: 4 days, 19 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
    #26610619 - 04/18/20 01:52 PM (2 months, 21 days ago)

Quote:

DezGWest7 said:
What's up guys. First timer here. I inoculated WBS March 27 in 3 Jars with GT. The thing is, i see NO activity in 2 of them and the one that I do see activity, I believe its probably contaminated but I'm not so certain. Here is a pic of it. I left it alone originally for the 1st 9 days or so until i decided to check it out and saw what the picture shows but smaller and yes, growth in those 2 different spots. I'm not sure where I f'ed up but *note- this WBS preparation did not include coffee grounds or gypsum. I rinsed , picked the floating grain off, rinsed until clear - soaked 20+ hours - simmered stovetop - then strained , spread out and left to dry a little over 2 hours(I recall it wasn't 100% dry when I decided to pack in the Jar for PS 120min Sterilization. Was it wet wet ? Noo. It just had enough moisture for me to notice when the grains were touching the inside of the jar after loading up. After the PC I allowed it to cool down more than 16 hours from my recollection & then I did the whole sterilize GT syringe etc inoculation of jars.





Feedback is appreciated.




Since no one gave me any feedback I waited to see how it would progress and I suppose it answered any questions I had. This is how it looks as of today


Edited by DezGWest7 (04/18/20 01:53 PM)


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OfflineWillieFunGuy
Stranger

Registered: 02/11/20
Posts: 34
Last seen: 23 days, 15 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
    #26622479 - 04/23/20 02:12 PM (2 months, 16 days ago)

Dude...looks perfect to me. I understand your trepidation though. I’m fairly new myself. Contams happen to the best of us. Keep it moving. Good job!


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OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 2,592
Loc: Over by your Mama's house Flag
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Re: [STICKY] Recognizing and dealing with contamination [Re: WillieFunGuy] * 1
    #26622501 - 04/23/20 02:18 PM (2 months, 16 days ago)

Give her a shake 👍


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Offlinenevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 1 day, 9 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: Crackatoa]
    #26623669 - 04/23/20 11:47 PM (2 months, 16 days ago)

Is this some stage of knots/primordia I've never noticed before or am I growing something freaky?


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Invisiblemurderlabz
Ich liebe Pilze
I'm a teapot

Registered: 05/18/19
Posts: 418
Loc: Frankfurt, Germany
Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita]
    #26623681 - 04/23/20 11:56 PM (2 months, 16 days ago)

Quote:

nevereataamanita said:
Is this some stage of knots/primordia I've never noticed before or am I growing something freaky?





Looks like it's building up Metabolites getting ready for war with something.


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Offlinenevereataamanita


Registered: 09/08/19
Posts: 121
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Re: [STICKY] Recognizing and dealing with contamination [Re: murderlabz]
    #26623689 - 04/24/20 12:03 AM (2 months, 16 days ago)

Damn, guess I'll keep an eye out and be ready to toss the shoebox. Here's a pic of the whole surface, I haven't spotted the contam yet.


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Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 19 days, 22 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita]
    #26624749 - 04/24/20 01:15 PM (2 months, 15 days ago)

Probably a bacteria, you won't really see it and you'll still get fruits.


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How I do glass dishes


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Offlinenevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 1 day, 9 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: poisoned]
    #26624761 - 04/24/20 01:19 PM (2 months, 15 days ago)

I've been wondering, is it a bad idea to clone or print from a bacterial grow? Is that shit going to get passed on or could you get away from it on agar?


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Offlinepoisoned
untitled
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Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 19 days, 22 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita] * 1
    #26624801 - 04/24/20 01:39 PM (2 months, 15 days ago)

usually fruits won't host other organisms on the inside.


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How I do glass dishes


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OfflinePinkStormtrooperS
Jet-Puffed


Registered: 04/11/20
Posts: 218
Loc: 10001110101 Flag
Last seen: 5 days, 1 hour
Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
    #26625847 - 04/24/20 10:40 PM (2 months, 15 days ago)

once they get 30% shake em like hell then leave em sit until they are 99% then plant those dudes. i cant tell you why its so slow tho. i could only suggest per reading your steps PC for 60min not 120min but looks good keep going.


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"say, you got a little astroglide on your moustache"


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Offlinejeezy1021
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Registered: 02/04/18
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Re: [STICKY] Recognizing and dealing with contamination [Re: PinkStormtrooper]
    #26628134 - 04/25/20 08:45 PM (2 months, 14 days ago)

does this look contaminated


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InvisibleMateah
The Adversary
I'm a teapot User Gallery
Registered: 07/14/16
Posts: 6,033
Loc: Venus
Re: [STICKY] Recognizing and dealing with contamination [Re: jeezy1021]
    #26628199 - 04/25/20 09:17 PM (2 months, 14 days ago)

It doesn't look great but there's one way to find out for sure and that's to fruit it, you can toss it when it becomes too obvious that it's contammed or if it doesn't produce any fruits.


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OfflineStonedTurtle
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Registered: 02/22/17
Posts: 53
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Last seen: 25 days, 23 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: Mateah]
    #26628507 - 04/26/20 12:15 AM (2 months, 14 days ago)

So there's one area on my jar that looks extremely white and the other jars lack this. The part I find crazy is that the area isn't growing. Theres a small area beside it, that I can tell is mycelium. Im assuming that stopped the growth of the extremely white part? I don't know.
I'm not sure if I should be worried,but outside opinions are always good
thanks for the help


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InvisibleStoneTroegs
Mycopath
Registered: 11/04/18
Posts: 20
Interesting Contam on Agar [Re: Kizzle]
    #26631378 - 04/27/20 04:59 AM (2 months, 13 days ago)



Can anyone ID the orange blob contam on my agar dish? It’s pretty cool looking and I’m trying to get more knowledge different growths


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InvisibleRyeHumorS
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 205
Loc: Yeast Of Knowhere
Re: Interesting Contam on Agar [Re: StoneTroegs]
    #26632150 - 04/27/20 01:05 PM (2 months, 12 days ago)

Quote:

StoneTroegs said:


Can anyone ID the orange blob contam on my agar dish? It’s pretty cool looking and I’m trying to get more knowledge different growths




The yellow blob is most likely bacteria, the reasons I say this are:

    (1) colorful contaminants are generally bacteria

    (2) it looks to be on the wet side, which is another indicator of bacterial growth.

your main culture itself, if it's supposed to be GT looks a bit funny too.

It's a little hard for me to tell from the image, but the fact that you have anything other than white myc is a bit of a red flag in my book.

Discoloration can be from metabolites or another fungi that you weren't looking to grow.

The beauty of doing your work on an agar dish is that you may still be able to transfer the best looking portion of the leading edge to a clean dish in order to leave the contamination behind.

That is assuming that your culture is still GT and not something else.

I have not done so myself yet but you can also mix things into the agar to inhibit bacterial growth. I think these include h202 agar and the antibiotic germentacin are examples.

I purchased the latter from one of the sponsors here.


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InvisibleStoneTroegs
Mycopath
Registered: 11/04/18
Posts: 20
Re: Interesting Contam on Agar [Re: RyeHumor]
    #26632368 - 04/27/20 03:12 PM (2 months, 12 days ago)

Thanks for the reply. I liked the look of the blob so I wanted to see if anyone knew the bacteria name. As for the GT I’m calling it a loss. I’ve got some good looking albino a+ and Puerto Rican ready for transfer.



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InvisibleRyeHumorS
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 205
Loc: Yeast Of Knowhere
Re: Interesting Contam on Agar [Re: StoneTroegs]
    #26632416 - 04/27/20 03:32 PM (2 months, 12 days ago)

Oh I see. Well it looks like apricot jelly...

wonder what it tastes like :grin:

And come to think of it that myc looks discolored similar to the appearance the foam on a saltwater fish tank protein skimmer looks.

That kind of brown rust orange fluff on the top.


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OfflineKkgal420
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Registered: 02/02/20
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Last seen: 15 days, 42 minutes
Is this contamination or new growth? [Re: StonedTurtle]
    #26632580 - 04/27/20 05:07 PM (2 months, 12 days ago)

I added verm/coir on the tops 9 days ago and I have white stuff growing on the tops. I can't figure out if it's contaminated or new growth from looking at the forums. What do y'all think?


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OfflineMindgmz
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Registered: 04/28/20
Posts: 2
Last seen: 5 days, 14 hours
Is this contamination? [Re: Kkgal420]
    #26635180 - 04/28/20 07:28 PM (2 months, 11 days ago)

First timer here, I am 3 weeks in and 9 jars almost completely colonized but I have this 1 I can't determine if it is contaminated or the dry verm slid down some. Any help is appreciated. Thanks


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