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kdsv
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Cobweb mold or just mycelium? [Re: Kizzle]
#27845143 - 07/01/22 03:00 PM (1 year, 6 months ago) |
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I'm trying LC to Agar for the first time. Do you think this is cobweb mold or just mycelium? It should be King Oyster.
I sprayed it a bit all over the dish, so it's also growing on the sides.
After 8 days

After 10 days

Same petri dish, just different angles.
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MushroomTattoo
Mushy Bussy



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Re: Cobweb mold or just mycelium? [Re: kdsv]
#27845770 - 07/01/22 09:20 PM (1 year, 6 months ago) |
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Looks mycelial so far but it's hard to tell
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Bvilleupallnight
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Registered: 12/06/15
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So, I am getting conflicting information and I'm wondering if you all can help. If this has been addressed already forgive me (this is a huge thread).
I have inoculated jars with growing mycellium, but the vermicilite around the white has a minor green tint to it. The company I bought the jars from (midwest grow kits) said throw them out, but I did 10 jars and 4 of them have this tint and I don't want to waste them unless I absolutely have to.
The images of contaminated jars in both their guide as well as images I have seen online do not look like this, and it makes me wonder if the seller of the jars is just being overly cautious for liability or something.
Could someone look at this pic and give their opinión on whether they can be saved?
https://files.shroomery.org/files/22-26/678314145-20220624_125833.jpg
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Smellyhobbit
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Those look super rough dude. I’d toss them.
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MushroomTattoo
Mushy Bussy



Registered: 04/19/22
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Those are definitely fucked! Toss 'em into a mix of Poo/verm/straw outdoors!
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Bvilleupallnight
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Thanks. I figured as much but would be pissed if they were actually ok. I had that happen to several of my jars. I have no idea what I could have done wrong, I used the oven method to inoculate.
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MushroomTattoo
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Bad filter / Bad seal /Improper pasteurization / dirty syringe / contaminated culture
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Bvilleupallnight
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Right on. I at least got three solid jars, and I'm only needing them for me and a couple friends. I'm sure the yield will be fine. Fingers crossed.
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invitro

Registered: 05/03/13
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Re: Cobweb mold or just mycelium? [Re: kdsv]
#27847926 - 07/03/22 05:11 PM (1 year, 6 months ago) |
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Quote:
kdsv said: I'm trying LC to Agar for the first time. Do you think this is cobweb mold or just mycelium? It should be King Oyster.
I sprayed it a bit all over the dish, so it's also growing on the sides.
After 8 days

After 10 days

Same petri dish, just different angles.
IME Oyster goes linear growth and cowbew goes crazed growth, looks like cobweb to me, just one guys opinion though, I'm not an oyster expert.
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#27850651 - 07/05/22 03:52 PM (1 year, 6 months ago) |
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Weird question, maybe someone out there has run into it.
I have a tub of spore to grain, dirty spawn. Sorta played the numbers to get some tissue samples, and the two that lived are obviously not awesome. One had trich, and this second one in question was obviously bacterial but otherwise actually doing fine once spawned.
Today I noticed a bruise in the myc, and three of the pins in that are were so loosely attached that I was able to simple reach in and remove them. The myc in that area is super fragile, but fine elsewhere.
Do you know what might have caused this? Surface conditions, temp, FAE are all right on the money. Visually it is no different from the rest of the tub, minus blue bruising that is common. What would make the myc so fragile?
They’re maybe 2 days from being ready to harvest, and it didn’t produce much anyway. I’m taking prints and some tissue for agar. Just curious if anyone knew why this might happen.
Edited by Smellyhobbit (07/05/22 03:52 PM)
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milkboy
Child



Registered: 05/01/21
Posts: 2,295
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#27850696 - 07/05/22 04:19 PM (1 year, 6 months ago) |
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Pictures are worth a thousand words
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#27850807 - 07/05/22 05:37 PM (1 year, 6 months ago) |
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I'm guessing here without seeing anything but, it's probably because of the bacterial spawn/contam. I had a good 4 flushes before my tub contaminated and then the fruits would fall over just from a slight shake of the tub. Before that I could pick up the entire substrate with holding a few fruits. My experience is limited so I could be way off.
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: milkboy]
#27850817 - 07/05/22 05:42 PM (1 year, 6 months ago) |
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Quote:
milkboy said: Pictures are worth a thousand words
I didn’t take a picture because visually it looks 100% healthy. I’ll give it 24 hours and maybe something will have developed that is worth looking at
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Bigworm]
#27850820 - 07/05/22 05:42 PM (1 year, 6 months ago) |
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Quote:
Bigworm said: I'm guessing here without seeing anything but, it's probably because of the bacterial spawn/contam. I had a good 4 flushes before my tub contaminated and then the fruits would fall over just from a slight shake of the tub. Before that I could pick up the entire substrate with holding a few fruits. My experience is limited so I could be way off.
It was definitely bacterial spawn, so that’s likely.
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milkboy
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit] 1
#27850841 - 07/05/22 06:01 PM (1 year, 6 months ago) |
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How can it look 100% visually healthy but you also know it to be bacterial spawn. Did you test a grain on agar or something? That makes no sense and further justifys the need for pictures lol.
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: milkboy]
#27850852 - 07/05/22 06:08 PM (1 year, 6 months ago) |
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Quote:
milkboy said: How can it look 100% visually healthy but you also know it to be bacterial spawn. Did you test a grain on agar or something? That makes no sense and further justifys the need for pictures lol.
I am 99% sure this was bacterial spawn. It was spore to grain. I’m having a lot of trouble with my agar right now so I’m just growing what I can with what I’ve got while I work on it. There was no doubt it was unhealthy spawn, I just was curious if this sounded like anything anyone had encounters, since visually it looks like very lightly bruised mycelium. I didn’t know there was even a problem until I moved the tub slightly and the loose pins wiggled.
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milkboy
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit] 3
#27850880 - 07/05/22 06:24 PM (1 year, 6 months ago) |
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From what Ive seen on my own grows usually if I see bruised mycelium on the substrate its because the surface got to dry, for example
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Bigworm



Registered: 03/19/22
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Re: Recognizing and dealing with contamination [Re: milkboy]
#27850896 - 07/05/22 06:30 PM (1 year, 6 months ago) |
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 And it seems to me it really shows up after misting. Almost like it's shocked it's getting water after being dry.
Edited by Bigworm (07/05/22 06:31 PM)
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Bvilleupallnight
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Re: Recognizing and dealing with contamination [Re: Bigworm]
#27850936 - 07/05/22 06:51 PM (1 year, 6 months ago) |
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Are these cakes salvegable?my fruiting chamber is clearly way too humid even though I drained perlite. I'm worried about green tint. Is this salvageable?
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Looks like the ugly green trich in my opinion. I would get that out of the lab ASAP and try not to disturb the spores of the trich. You don't want that spreading and getting into future grows. You can wait for a second opinion if you want.
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