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OfflineDERRAYLD
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Re: Recognizing and dealing with contamination [Re: Sill] * 1
    #27843329 - 06/30/22 04:24 AM (1 year, 6 months ago)

Judging by the location of the contamination it could be filter failure.

What filters are you using and what did the culture look like?


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OfflineSill
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Re: Recognizing and dealing with contamination [Re: DERRAYLD] * 1
    #27843344 - 06/30/22 04:50 AM (1 year, 6 months ago)

Thanks DERRAYLD for the quick response šŸ™

The darkening started at the top but I saw some dark spots at the bottom of the jar as well.

I'm curious to know, what kind of contamination is this?

Regarding the filter, I’m not sure what is the filter but here is a picture of it



You reminded me that during pressure cooking the jars the smell of the grain was coming out of the pressure cooker, that didn’t seem right to me!


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OfflineSmellyhobbit
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27843346 - 06/30/22 04:52 AM (1 year, 6 months ago)

Is that not normal? I usually smell a little of whatever I’m PCing. I use polyfill filters and they’re not air tight. I assumed that was expected.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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OfflineSill
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #27843351 - 06/30/22 04:58 AM (1 year, 6 months ago)

I don’t know tbh. This is my first time so I’m just assuming šŸ˜…


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OfflineDERRAYLD
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Re: Recognizing and dealing with contamination [Re: Sill]
    #27843353 - 06/30/22 05:02 AM (1 year, 6 months ago)

If there's contamination lower down then I will assume this is a case of dirty agar or insufficient pc'ing.


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OfflineSill
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Re: Recognizing and dealing with contamination [Re: DERRAYLD]
    #27843357 - 06/30/22 05:14 AM (1 year, 6 months ago)

Any idea what kind of contamination is it?


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Invisiblepablokabute
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Re: Recognizing and dealing with contamination [Re: Sill]
    #27843361 - 06/30/22 05:17 AM (1 year, 6 months ago)

Quote:

Sill said:
Any idea what kind of contamination is it?




Its a black mold. you can take out a colonized grain away from the mold and then put to agar to clean it up. It might take a couple of transfers but atleast you dont wait for germination if starting from spores a gain


--------------------

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OfflineSill
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Re: Recognizing and dealing with contamination [Re: pablokabute]
    #27843436 - 06/30/22 06:36 AM (1 year, 6 months ago)

How should I dispose of the grains? What’s the safest way to do it without health risk or contamination of the grow space?


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OfflinePBJ710
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Re: Recognizing and dealing with contamination [Re: Sill]
    #27843441 - 06/30/22 06:43 AM (1 year, 6 months ago)

In the trash or a compost pile.  There's billions of mold spores already in your home that you are already breathing daily - it's no different than throwing away moldy bread.


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OfflineSoZo
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Something Riding Along? [Re: Kizzle]
    #27843551 - 06/30/22 08:36 AM (1 year, 6 months ago)

All my cultures look like these: a mixture of tomatose and rhizomorphic growth.  Is the fuzzy stuff a contaminant?




--------------------
Curiosity killed the cat
Satisfaction brought it back


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InvisibleCulliganman360
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Re: Something Riding Along? [Re: SoZo]
    #27843841 - 06/30/22 01:38 PM (1 year, 6 months ago)

Sozo tough to say man.but looking at the last pic it most likely is something unwanted riding along
Could be why your seeing such thick ropes
that myc trying to hurry the fuck away

I would make transfers from the leading edge of the ropes in hopes you leave the tomentose shit behind .

After transfe throw it to some grain and see what it does for experimental purposes. If u want


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Invisibleoutlier52
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Re: Something Riding Along? [Re: Culliganman360]
    #27844139 - 06/30/22 05:42 PM (1 year, 6 months ago)

These are both 43 days out and haven’t made any headway with the grains on the outside of the jar. Both inoculated with LCs from clones. What thinks you?






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InvisibleGuerrilla
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Re: Something Riding Along? [Re: outlier52]
    #27844184 - 06/30/22 06:38 PM (1 year, 6 months ago)

Quote:

outlier52 said:
These are both 43 days out and haven’t made any headway with the grains on the outside of the jar. Both inoculated with LCs from clones. What thinks you?









Bacterial


--------------------
Being pissed on does not make you a real man.


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Invisibleoutlier52
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Re: Something Riding Along? [Re: Guerrilla]
    #27844192 - 06/30/22 06:49 PM (1 year, 6 months ago)






Bacterial




Out they go then. Thanks.


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Offlinecozmyc
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Re: Something Riding Along? [Re: outlier52]
    #27844244 - 06/30/22 07:47 PM (1 year, 6 months ago)

I'd spawn separately or in an outdoor pot or bed. Something's better than notta :mushroom2:


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You're conscious population 2 stardust
----------------------
and that's valuable :sunny:


Edited by cozmyc (06/30/22 07:48 PM)


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Invisiblepablokabute
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Re: Something Riding Along? [Re: outlier52] * 1
    #27844359 - 06/30/22 09:37 PM (1 year, 6 months ago)

Quote:

outlier52 said:
These are both 43 days out and haven’t made any headway with the grains on the outside of the jar. Both inoculated with LCs from clones. What thinks you?










43 days sounds like a looooooooooooooooooooooooong time.

1-3weeks is more like it. But ye, they dont look good. I bet they smell way off too.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: ā€œAll CONTAMINATED things and events are unsatisfactory.ā€'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


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InvisibleCulliganman360
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Re: Something Riding Along? [Re: outlier52]
    #27844421 - 06/30/22 11:42 PM (1 year, 6 months ago)

Quote:

outlier52 said:
These are both 43 days out




:holyfuckdude:


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OfflineMushroomTattoo
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Re: Recognizing and dealing with contamination [Re: Shaker77]
    #27844689 - 07/01/22 07:51 AM (1 year, 6 months ago)

If there is any mycelium at all, mix it with pasteurized h/poo and put outdoors. Manage the conditions (heat, humidity) and you might just get lucky. I always mix contaminated substrate in my garden or flower pots and at least get a fruit or two.

Here's some pinning from badly contam'd grains mixed with newspaper/poo/verm



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Invisibleoutlier52
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Re: Something Riding Along? [Re: pablokabute]
    #27844697 - 07/01/22 07:59 AM (1 year, 6 months ago)

Quote:

pablokabute said:
Quote:

outlier52 said:
These are both 43 days out and haven’t made any headway with the grains on the outside of the jar. Both inoculated with LCs from clones. What thinks you?










43 days sounds like a looooooooooooooooooooooooong time.

1-3weeks is more like it. But ye, they dont look good. I bet they smell way off too.




Oddly enough, there was no detectable odor. But it didn’t smell like mycelium, either. I buried them nonetheless.

Here’s hoping for a backyard miracle.


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OfflineSoZo
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Re: Something Riding Along? [Re: Culliganman360]
    #27844847 - 07/01/22 10:55 AM (1 year, 6 months ago)

Thanks for the reply.  The truth is that I have taken many transfers from the leading growth already.  I have used some to inoculate grain (oats).  Some of the grain turns out okay, some is lost to contamination.  The grain goes into shoeboxes with coir.  I get a good first crop but usually see trich with the second.


--------------------
Curiosity killed the cat
Satisfaction brought it back


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