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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,868
Loc: Murica Flag
Re: Recognizing and dealing with contamination [Re: Headspace111]
    #27818871 - 06/14/22 05:55 AM (1 year, 7 months ago)

I would dump it. Sitting water is a breeding ground for bacteria.


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OfflineAverroes
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Registered: 02/18/22
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27819688 - 06/14/22 05:29 PM (1 year, 7 months ago)

I am dealing with a little cobweb mold in the corner of my tray, but this is something I haven't seen.  I had a relatively successful BRF trial and a moderately successful bulk with B+ (approx. 420 wet).  But what about this?  Looks terrible.  These are Burma and the primordia are thick.



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InvisibleBigwormS
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Registered: 03/19/22
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Loc: Murica
Re: Recognizing and dealing with contamination [Re: Averroes]
    #27819849 - 06/14/22 07:32 PM (1 year, 7 months ago)

It looks a little thick to be cobweb. Look at some other pictures of cobweb mold. I would say it's just bruised mycelium. I'm guessing it showed after misting? Although your sub looks a little dry to me. I like to see little beads of water on the surface of my tubs, until the little guys get a little bigger than what you have there, then I stop misting until after harvest. First flush on this one?


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OfflineNichrome
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Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27820084 - 06/14/22 10:12 PM (1 year, 7 months ago)

All I see is bruising, a little bit of metabolite stain, and a lot of pins.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineAverroes
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Re: Recognizing and dealing with contamination [Re: Nichrome]
    #27820401 - 06/15/22 06:50 AM (1 year, 7 months ago)

Thanks guys!  I wasn't clear in my op.  The cobweb is in another corner.  I think I have that under control.  I now think the current photo is bruising.  It ironically shows better on the picture.  I also think my slab is too dry, which might have caused the bruising and staining.  My water/misting game has been off.


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OfflineCorey
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Registered: 02/28/22
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Re: Recognizing and dealing with contamination [Re: Averroes]
    #27822098 - 06/16/22 07:34 AM (1 year, 7 months ago)

First time grower here. Could someone tell me what these dots are on two out of five of my jars? It's been really hot and humid here the last few days and I just noticed them today when I got up and was looking at them. They are PF Tek inoculated with spore syringes on 5/28. I know spore syringes aren't the best. I've been buying stuff to start doing Agar. Hopefully I uploaded these pics correctly.



Edited by Corey (06/16/22 07:35 AM)


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OfflineHindsight
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Re: Recognizing and dealing with contamination [Re: Corey]
    #27822116 - 06/16/22 07:48 AM (1 year, 7 months ago)

Looks like bacterial infection and/or metabolites secreted due to bacterial infection. That amount is quite small. Wouldn't worry about it because there isn't anything you can do. Just continue on and fruit the cakes and see what you end up with.


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OfflineCorey
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Re: Recognizing and dealing with contamination [Re: Hindsight]
    #27822126 - 06/16/22 08:00 AM (1 year, 7 months ago)

Thank you. I'm just gonna do Bod's Easy AF cheapest way to get started TEK and throw some bags over them and fruit them that way. I appreciate you helping me out. Should I quarantine them in another room?


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OfflineHindsight
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Re: Recognizing and dealing with contamination [Re: Corey]
    #27822141 - 06/16/22 08:12 AM (1 year, 7 months ago)

No, bacterial contamination, if that is what it is, isn't much to worry about. Just follow the dunk and roll and then fruit them. I wouldn't worry about isolating them.


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OfflineCorey
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Re: Recognizing and dealing with contamination [Re: Hindsight]
    #27822150 - 06/16/22 08:27 AM (1 year, 7 months ago)

Thanks! Appreciate you.


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OfflineNichrome
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Registered: 12/17/18
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Re: Recognizing and dealing with contamination [Re: Corey]
    #27822301 - 06/16/22 10:44 AM (1 year, 7 months ago)

Cobweb mold "under control" is as frequent as the common jackelope.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Offlinebaldur
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Registered: 06/30/21
Posts: 434
Last seen: 24 days, 4 hours
Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27829172 - 06/20/22 07:36 PM (1 year, 7 months ago)

Are my shoeboxes contaminated, or to wet, or just normal?

It's just that I'm seeing some funny dark stuff at the top, and the mycelium in that area is not showing any signs of water droplets forming. Could that be an unwanted species sneaking in, or just really wet Golden Teacher mycelium?





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InvisibleBigwormS
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Registered: 03/19/22
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Loc: Murica
Re: Recognizing and dealing with contamination [Re: baldur]
    #27829224 - 06/20/22 08:06 PM (1 year, 7 months ago)

That doesn't look good at all to me.


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Offlinebaldur
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27829881 - 06/21/22 06:16 AM (1 year, 7 months ago)

Yeah I'm wondering if I should throw it out (if it's contamination), or try to dry it up a bit by introducing FAE (if it's just excess moisture).

Kinda looks like mold at it's first stages. But I'm no expert.


Edited by baldur (06/21/22 06:17 AM)


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InvisibleBigwormS
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Registered: 03/19/22
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Re: Recognizing and dealing with contamination [Re: baldur]
    #27830534 - 06/21/22 02:46 PM (1 year, 7 months ago)

I would trash it. I hate saying that to people and I hate having to do that myself even more, but that's how things go in this line of work.


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InvisibleWyoMX
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Registered: 07/06/15
Posts: 2,100
Loc: PNW
Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27830634 - 06/21/22 04:24 PM (1 year, 7 months ago)

The yellow is just metabolites. It's not the end of the world but it means there's likely bacteria the mycelium is fighting. It may still fruit but your chances of getting more then one flush are slim. When the mycelium is fighting off bacteria it weakens itself and is more susceptible to molds and whatnot. I wouldn't trash that yet but don't get your hopes up to high. There is still a possibility of fruits for sure though. This shoebox of mine had metabolites but still fruited nicely and ended up with more then an oz off 1 quart of spawn. 



Edited by WyoMX (06/21/22 04:25 PM)


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OfflineNichrome
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Registered: 12/17/18
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Re: Recognizing and dealing with contamination [Re: WyoMX]
    #27830650 - 06/21/22 04:35 PM (1 year, 7 months ago)

Just pop the plastic off the side of the mycelium so the condensation can run down and so it has more surface area to breathe. You can still get fruits from that although it is less than perfect.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineMadshroomz
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Re: Recognizing and dealing with contamination [Re: Nichrome]
    #27831016 - 06/21/22 09:16 PM (1 year, 7 months ago)
Log in to view attachment

Hello everyone
It's been a while since I am trying to grow Psilocybe azurescens agar but what I get is like the image! I am very new to mushroom word :smile: So I am not sure if this is contamination or weeping! I use a lab autoclave and work under BSC. I would appreciate it if you let me know your opinions.

BTW IDK who to add the image here! I've attached a pfd file :smile:




Edited by Madshroomz (06/21/22 09:25 PM)


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Offlinebaldur
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Registered: 06/30/21
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Re: Recognizing and dealing with contamination [Re: Nichrome]
    #27831668 - 06/22/22 12:24 PM (1 year, 7 months ago)

Quote:

Nichrome said:
Just pop the plastic off the side of the mycelium so the condensation can run down and so it has more surface area to breathe. You can still get fruits from that although it is less than perfect.




I don´t really know what you mean by “pop the plastic off the side of the mycelium…”

Care to elaborate a bit?


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OfflineB Traven
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Re: Recognizing and dealing with contamination [Re: Nichrome]
    #27832809 - 06/23/22 10:43 AM (1 year, 7 months ago)

Quote:

Nichrome said:
Cobweb mold "under control" is as frequent as the common jackelope.




Whatever do you mean?

I successfully control cobweb all the time with salt and H2O2.

It's usually only an issue when I run out of lysol for spraying the walls of my SAB, though.


--------------------
Beware of advice- even this.


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