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Offlinemoshe1

Registered: 08/29/15
Posts: 378
Last seen: 1 year, 7 months
Re: Recognizing and dealing with contamination [Re: Joseph Nerino]
    #27805920 - 06/04/22 04:32 PM (1 year, 7 months ago)

quick question guys, no pic because theres nothing visual. The bag looks to be 100% clean and colonized, but the grains fall apart with almost no effort. Never had a bag do this before, im looking into the grains to notice any sort of oddities

Recognize anything fishy in any of these pics?



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Edited by moshe1 (06/04/22 04:32 PM)


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InvisibleSmartattack
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Registered: 12/21/18
Posts: 3,775
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Re: Recognizing and dealing with contamination [Re: moshe1] * 2
    #27806030 - 06/04/22 06:33 PM (1 year, 7 months ago)

I'm thinking that your standard of clean is off. All the slimey areas and wet looking grain is all the sign you need to know that spawn isn't primo. Spawn like that is always a dice roll.


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Offlinemoshe1

Registered: 08/29/15
Posts: 378
Last seen: 1 year, 7 months
Re: Recognizing and dealing with contamination [Re: Smartattack]
    #27806229 - 06/04/22 09:35 PM (1 year, 7 months ago)

Gotcha thanks for the reply.

If im being honest i've had a decent number of bags have pooling water over the past years rounds that ended up spawning fine, never really figured it out.

Quote:

cronicr said:
it doesn't, the water is just from it being over colonized, they put off soem heat which causes condensation which pools up, no biggie no threat.




These bags have been sitting 100% colonized for a while for what its worth. I tend to look out for other signs besides extra water nowadays to be sure i have something nasty in there because now im never too sure and i want to end this cycle lol.

I have a room dedicated for labwork with a sizable flowhood- I shouldnt have any excuses tbh. What is causing all this extra moisture? Literally clear water almost in some cases, most of the time slightly yellow mixed with metabolite

I follow the most traditional teks and nothing is changed in my process either.

Couldnt excess water in a clean bag produce slime? I could fruit them without using any sub right?


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Edited by moshe1 (06/04/22 10:09 PM)


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Offlinesubmedusa
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Registered: 05/10/22
Posts: 6
Last seen: 1 year, 7 months
Re: Recognizing and dealing with contamination [Re: moshe1]
    #27806330 - 06/04/22 10:42 PM (1 year, 7 months ago)

Clearly my first agar attempts were not done correctly as their are contams all over the place! I used a cutting from inside a stem - what is the slime underneath it? Trying to figure out the best way to transfer it to a clean agar plate.



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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,868
Loc: Murica
Re: Recognizing and dealing with contamination [Re: submedusa]
    #27806358 - 06/04/22 11:02 PM (1 year, 7 months ago)

It could be moisture from the mushroom leaking out onto the agar but it looks bacterial to me. Was the grow clean, or was it showing signs of contamination? Clearly you were not in an ideal location for agar work so maybe you transferred some bacteria when taking the sample, even from inside tissue.


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InvisibleSmartattack
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Re: Recognizing and dealing with contamination [Re: submedusa] * 2
    #27806677 - 06/05/22 07:52 AM (1 year, 7 months ago)

Quote:

submedusa said:
Clearly my first agar attempts were not done correctly as their are contams all over the place! I used a cutting from inside a stem - what is the slime underneath it? Trying to figure out the best way to transfer it to a clean agar plate.







Not all plates necessarily should be worked with. This looks like a good example of just trying again probably being more efficient.


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OfflineLazyLightningBug
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Registered: 09/29/21
Posts: 24
Loc: Here, Now.
Last seen: 1 month, 6 days
Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27806781 - 06/05/22 09:37 AM (1 year, 7 months ago)

Keeping a lower profile.


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Cultivating my relationship with our fungal partners


Edited by LazyLightningBug (06/08/22 11:35 PM)


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Invisiblemilkboy
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Registered: 05/01/21
Posts: 2,295
Re: Recognizing and dealing with contamination [Re: LazyLightningBug] * 1
    #27807052 - 06/05/22 01:50 PM (1 year, 7 months ago)

Just mushrooms doing mushroom things, ms grows can have alot of variation.


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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,868
Loc: Murica
Re: Recognizing and dealing with contamination [Re: LazyLightningBug]
    #27807121 - 06/05/22 02:59 PM (1 year, 7 months ago)

I would get a clone of the leucistic cluster ones and of the big one from the normal cluster. Try growing them out again and see if you get the same results. I do not see any signs of vert. Good job!


Edited by Bigworm (06/05/22 03:00 PM)


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OfflineLazyLightningBug
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Registered: 09/29/21
Posts: 24
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Re: Recognizing and dealing with contamination [Re: milkboy]
    #27807183 - 06/05/22 04:13 PM (1 year, 7 months ago)



Thanks y’all


--------------------
Cultivating my relationship with our fungal partners


Edited by LazyLightningBug (06/08/22 11:36 PM)


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Offlinesubmedusa
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Registered: 05/10/22
Posts: 6
Last seen: 1 year, 7 months
Re: Recognizing and dealing with contamination [Re: Smartattack]
    #27807639 - 06/05/22 10:13 PM (1 year, 7 months ago)

Yeah I think I'm just gonna toss 'em all and start over, much more conscious this time! The stem was from a wild mushroom which may have contributed.


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OfflineHeadspace111
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Registered: 03/11/22
Posts: 63
Loc: Event Horizon of a black hole
Last seen: 1 year, 5 months
Re: Recognizing and dealing with contamination [Re: milkboy]
    #27810361 - 06/08/22 12:30 AM (1 year, 7 months ago)

Hey I have 12 shoeboxes right now and discovered 2 of them might have trich. Well definitely looks like it. On the bottom of the box the colonized substrate is starting to turn yellowish and I found a few green spots on the sides. The two boxes in question are about a week from finishing the first flush. Should I separate them and keep them going as long as the mush isn't compromised? I used Damian5050 coir tek and followed SharperDreamings SFF shoebox tek. I haven't had any bad spawn bags but I mixed the spawn and substrate in an open garage on the floor (stupid) so is this where I could have fucked myself with the dust thus creating the trich? I also used a 1:1.5 ratio which i read is more apt to contamination anyway. All my other boxes I used a 1:2 ratio and they seem to be fine right now. What can I do or is there nothing to do and just collect the first flush then throw out? Also with it being a set and forget tek which doesn't need opening do I risk contamination of the other boxes by keeping them stacked together?







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InvisibleBigwormS
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Registered: 03/19/22
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Loc: Murica
Re: Recognizing and dealing with contamination [Re: Headspace111]
    #27810979 - 06/08/22 03:29 PM (1 year, 7 months ago)

I'm not a super experienced person so take what I say with the smallest grain of salt but are you sure it's trich? Some looks a little yellow and the first looks a bit blue. Maybe metabolite for yellow ones and bruised side pins for the blue? If you are sure it's contaminated, since I'm looking at pics and you are looking at the real deal, I would move the boxes that are infected to a different location if you are dead set on getting a first flush from them.  I'm not that familiar with the neglect tek but have read over it quickly before. If you have any FAE going on and they are around the other boxes there is a chance of spores escaping and going into the other boxes. Hopefully it turns out ok for you.


Edited by Bigworm (06/08/22 03:31 PM)


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OfflineHeadspace111
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Registered: 03/11/22
Posts: 63
Loc: Event Horizon of a black hole
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27811637 - 06/09/22 04:09 AM (1 year, 7 months ago)

So I looked at them again yesterday and the metabolites are definitely there but the green spots are a very dark green but ita very tiny. It doesn't look like its spreading through the substrate. It's definitely not on the top layer and it only started to show up a few days ago. I made these boxes on 5/26. The Rusty Whyte variety im running are looking like blobs. Now I'm not sure but I think i read that blobs can be caused by some sort of contams? I do have the aerial rhizomorphs, and I read that trich also looks like that cottony fuzz on top of the substrate.  If it is trich is not really growing too fast.


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InvisibleBigwormS
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Re: Recognizing and dealing with contamination [Re: Headspace111]
    #27811699 - 06/09/22 05:55 AM (1 year, 7 months ago)

Just my opinion. I'm always cautious and move anything I think is contaminated out of the grow area. I've had trich 1 time and it didn't spread to my other boxes so moving that 1 helped. I would say if it's dark green it probably is trich and the spores are wet which will help them not be airborne. For a short answer, if you want to try to salvage it, I'd get it out of the area. If you let it grow do not open it in the building you grow in. Anywhere in the building! Do not open them then open the non contaminated containers. Get a shower and wash the clothes you were wearing before opening the others. The spores can spread and effect future projects for a long time. Even if you don't see them spreading they might be.  Good luck!


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OfflineLivingSpaces
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Registered: 04/08/22
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Loc: Los Angeles
Last seen: 1 month, 20 days
Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27813713 - 06/10/22 05:44 PM (1 year, 7 months ago)

This is my first PF Tek grow and I have been reading a lot of posts up to this point, but I wonder if some could chime in.  I don't know what I"m looking at.  Is it contam (green mold) or just bruising in the first photos?  The cakes were birthed 8 days ago, after dunking for 24h and rolling.  I have 2 SGFCs.  3 days ago I tossed out 4 cakes because of what I suspected to be green mold.  Now, I think it may have just been mycelium colonizing the rolled vermiculite and bruising, but I don't know? 
Also, do I need more holes in my sides?  I tried to do a 2" grid pattern but think I may need more...
I love this hobby.




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InvisiblefahtsterM
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Registered: 06/17/06
Posts: 9,266
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Re: Recognizing and dealing with contamination [Re: LivingSpaces] * 1
    #27813887 - 06/10/22 08:45 PM (1 year, 7 months ago)

Are you misting them directly?  That can cause matting if the myc and bruising.  Drying out also causes bruising.  Sgfc’s are notorious for drying cakes out. 

That tub is just too big with too big of holes in it.  I suspect they’re drying out.  You can literally grow a cake in a sandwich baggie with some needle holes poked in it and get a great flush


Cakes do really well with only 2-3” of space around them.. it’s less space to maintain and syringe needle holes placed like a sgfc work well because they’re more restrictive.  You can do multiple cakes in a bigger ziplock with perlite

For reference Growing with ziplocks

Or use a Water tub

Your fruits do look dry. Check out Bottom watering to replenish the cakes water supply without disrupting the progress of the flush (dunking or misting directly<—very ineffective and disruptive to the evaporation cycle of cake/fruit ime)

You could get way more cakes in a tub that size to try and keep to that 2-3” space.. really pack em in.  Here’s 24 cakes in each of these 54qt tubs (double stacked towers—not a sgfc)


Faht


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OfflineLivingSpaces
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Registered: 04/08/22
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Re: Recognizing and dealing with contamination [Re: fahtster]
    #27814025 - 06/10/22 10:53 PM (1 year, 7 months ago)

Yes, today I added water to the aluminum trays and the cakes sucked up the water quick.  I had read about bottom watering, which I thought said to water after pinning, but there has been no pinning (probably because too dry..). When I bottom watered the one with the fruits, it sucked up at least 50mL.  Everything's prolly been drying out in the SGFC.  I'll check out growing with ziplocks like you suggest.  Until then, maybe I'll put everything I have now in one SGFC, spaced closer to each other, and cover some holes with micropore tape?
I work 12hr shifts at night so I'm unable to tend to them for this grow as much as I would like.


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InvisiblefahtsterM
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Re: Recognizing and dealing with contamination [Re: LivingSpaces]
    #27814026 - 06/10/22 10:54 PM (1 year, 7 months ago)

Your plan sounds good.. I’d mist that perlite down daily.. don’t worry about a little pooling


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OfflineHeadspace111
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Registered: 03/11/22
Posts: 63
Loc: Event Horizon of a black hole
Last seen: 1 year, 5 months
Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27818742 - 06/14/22 01:25 AM (1 year, 7 months ago)

Thanks man. Everything seems fine. No growth, and when I moved the cake to harvest they were gone. Weird... I'm thinking it was a very bruised pin on the side. But now I have another question. As an experiment I had some horse poo and I mixed with with my CV. The water is now sloshing around the bottom of the shoebox and I'm not sure if it's a good idea that it's sitting in there. The substrate has already caked up and it seems to have all the moisture it needs and isn't soaking the poo water up anymore. Keep or dump 🤔 ahaha dump. Ok enough..


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