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InvisibleCulliganman360
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Re: Recognizing and dealing with contamination [Re: anunnaki]
    #27797763 - 05/29/22 01:23 PM (1 year, 7 months ago)

Looks sus but usually all germ plates look sus. You can take a transfer from the leading edge now
Or give it a few more days to grow out .
I’ve noticed if I let germ plates sit for awhile they either throw some ryzo growth or start pinning


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InvisibleBigwormS
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Re: Recognizing and dealing with contamination [Re: anunnaki]
    #27798182 - 05/29/22 06:43 PM (1 year, 7 months ago)

I'm new to the whole agar thing so feel free to ignore me, but IME I would make some transfers now from the 5-8 o clock areas on the first pic. Just the leading edge of the growth. Make a few plates from those sections so if one is contaminated you have back up plates. If you have extra plates take one or two samples from the opposite side as an experiment just to see what happens.


Edited by Bigworm (05/29/22 06:43 PM)


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InvisibleBigwormS
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Re: Recognizing and dealing with contamination [Re: Aus404]
    #27798193 - 05/29/22 06:51 PM (1 year, 7 months ago)

I would like to say severe bruising from dryness but it looks like more going on than that. From what my untrained eye can see it looks like it's screwed. If it's powdery get it out of the house ASAP as you don't want the spores to spread.


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OfflineBeardedAngler
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27798222 - 05/29/22 07:25 PM (1 year, 7 months ago)

This is my first time using agar and have managed to produce something that I cannot find photos similar too.  Plate was inoculated from a syringe, even though my drop turned into a bit of a squirt.  This plate is 6 days old, and my other plates have not shown any growth yet.  There are small dots around the edge that look correct to me, but I am unsure about the rest.  It almost looks more like a LC on the agar.  Could this be from excess solution, or is it contamination?  Any help or experience is appreciated. 



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Invisiblemilkboy
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Re: Recognizing and dealing with contamination [Re: BeardedAngler]
    #27798242 - 05/29/22 07:41 PM (1 year, 7 months ago)

That is bacteria


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OfflineBeardedAngler
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Re: Recognizing and dealing with contamination [Re: milkboy]
    #27798247 - 05/29/22 07:44 PM (1 year, 7 months ago)

Thanks, I had a feeling it was as it was not uniform, but had not seen any similar photos to verify it


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Invisiblemilkboy
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Re: Recognizing and dealing with contamination [Re: BeardedAngler]
    #27798257 - 05/29/22 07:51 PM (1 year, 7 months ago)

Heres a pic from when I first started out, mycelium, mold, and bacteria all on one plate.


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OfflineBeardedAngler
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Re: Recognizing and dealing with contamination [Re: milkboy]
    #27798264 - 05/29/22 07:59 PM (1 year, 7 months ago)

That is helpful.  I have done cakes, but it has been like 15 years ago.  I knew it was off, but wanted to double check with those more experienced.


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Invisiblemilkboy
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Re: Recognizing and dealing with contamination [Re: BeardedAngler]
    #27798275 - 05/29/22 08:13 PM (1 year, 7 months ago)

Agar is the shit, youll get an eye for it in no time. Becomes an obsession all of its own.


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OfflineAus404
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27798311 - 05/29/22 08:41 PM (1 year, 7 months ago)

The "bruising"  itself isn't powdery and doesn't come off with a Qtip. But the caps have a wet feeling to them with a white substance, which I did accidentally over water the block for about 4 days before I learned better about wetting the block.

I should toss this out then?:



Edited by Aus404 (05/29/22 08:52 PM)


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InvisibleBigwormS
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Re: Recognizing and dealing with contamination [Re: Aus404]
    #27798345 - 05/29/22 09:06 PM (1 year, 7 months ago)

If it's not trich, which would be powdery, let it ride and see what happens. The wet feel isn't a big deal. The white caps I've had before didn't get much bigger but I've eaten them without adverse effects. I'm not advising you to eat them just giving my experience. Some of the fruits look ok so you might be able to get a small harvest from it.


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OfflineAus404
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27800106 - 05/31/22 10:44 AM (1 year, 7 months ago)

Update on it.

They are looking good. Never seen the white slimy stuff though that is on the caps. It wipes right off though. I'll likely wipe them all down before sticking them in the dehydrator. Probably be good for harvesting Thursday.



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InvisibleBigwormS
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Re: Recognizing and dealing with contamination [Re: Aus404] * 1
    #27800196 - 05/31/22 11:35 AM (1 year, 7 months ago)

At least it wasn't a total loss. Don't forget to get a clone and or a print for future use.


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Offlinesmalltalk_canceled
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Re: Recognizing and dealing with contamination [Re: Bigworm] * 1
    #27800348 - 05/31/22 01:19 PM (1 year, 7 months ago)

Lol a clone/print from that


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InvisibleGuerrilla
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Re: Recognizing and dealing with contamination [Re: smalltalk_canceled]
    #27800352 - 05/31/22 01:22 PM (1 year, 7 months ago)

Quote:

smalltalk_canceled said:
Lol a clone/print from that




He tried

:lol: :lol: :lol:
:canthelpbutlaugh:
:bathtub40lol::loldongs:


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OfflineAus404
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Re: Recognizing and dealing with contamination [Re: Guerrilla]
    #27800628 - 05/31/22 04:56 PM (1 year, 7 months ago)

Sorry for posting so much on this.

I'm still unsure if this looks okay or not, being new to this. It's just that it looks nothing like the B+ I grew, with this having that white film on it.

You can see in these pictures the spots where I took a paper towel and wiped the film off (and bruised a couple spots doing so). The last one bruised blue but this Ajax bruises green I suppose? Just trying to make sure I'm safe about everything.

Like I said I believe I overdid it on the misting early on.





EDIT: Judging by these photos online of Ajax, maybe they are fine and this is just a normal look for the strain and that white film substance is normal?:



Edited by Aus404 (05/31/22 05:09 PM)


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Invisiblemilkboy
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Re: Recognizing and dealing with contamination [Re: Aus404] * 1
    #27800654 - 05/31/22 05:15 PM (1 year, 7 months ago)

Harvest, dry, eat, they are fine. The white on the cap is from the universal veil.


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Offlinesmalltalk_canceled
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Re: Recognizing and dealing with contamination [Re: milkboy]
    #27800697 - 05/31/22 05:45 PM (1 year, 7 months ago)

One one two, do what you gotta do
You can't fight the green
Shits madlib, obscene
So don't take a print from the green Goblin's cake
It'll lead to death 'n be your final mistake.

Maybe make a swab
  Send it for a sporeprint real tell the mirror you upheld
your end o da deal

*swabs die and people stop using them*

Checkmate, swabs


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OfflineB Traven
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Re: Recognizing and dealing with contamination [Re: smalltalk_canceled] * 1
    #27800753 - 05/31/22 06:41 PM (1 year, 7 months ago)

My personal belief is basically that fruits are fine to eat unless they look/feel/smell repulsive to you. Like, not in a "this is a wierd new food I'm trying" way, but in a "this shit is slimy and gross and makes me gag" way. If there's no external or internal bruising or rot on a fruit, then I don't think twice.

I generally won't pick and dry fruit from containers with visible green trich or other spores. But that's not really about eating them, it's just that I don't want to kick up a bunch of invisible spores in my space during the process of picking them and running the dehydrator. If you're not running continuous grows in your space, or only doing PF cakes, then it might not be as much of an issue.

White fuzz on fruits, with no other symptoms, is almost always just cubensis myc acting weird.


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OfflineJoseph Nerino
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Re: Recognizing and dealing with contamination [Re: B Traven]
    #27802352 - 06/02/22 07:10 AM (1 year, 7 months ago)

From the behavior and colors is created, I'm pretty sure my first tub ever had fusarium contam. If you read into it, fusarium has some links to spinal meningitis, so please remember your mask dealing with potential contamination(s)

I even recently tried cutting out some green and spraying ammonium chloride directly on the walls of the removed section and the green demon came back with a vengeance lol

Here's a couple pics of contams I've had..



*My recent grain bag won't upload but it was green and fuzzy within 48hrs of inoculating


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