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OfflineAus404
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Registered: 03/28/22
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Re: Recognizing and dealing with contamination [Re: pablokabute]
    #27796878 - 05/28/22 10:21 PM (2 months, 21 days ago)

This is only my second large block grow so I'm unsure if what I am seeing is mold. This is Ajax and it seems to be localized to the top of the block.

Is this mold/trichoderma and if so is this salvageable? Can I chop off the top of this block to save the rest if it is? Or is this just bruising/dehydration/normal?



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Offlineanunnaki
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Re: Recognizing and dealing with contamination [Re: Aus404]
    #27797756 - 05/29/22 03:15 PM (2 months, 20 days ago)

This is my first time germinating spores on agar. I am using some a fairly old print that I made a syringe out of.

I used 1 drop in the center on relatively soft BRF agar (6g BRF / 7 agar / 400ml Water)

I'm not sure whether I am looking a mycelium or some kind of cobweb. The reason I am suspecting contamination is because only this out of 20 plates have shown any growth. This is after 9 days since inoculation.

Any help would be greatly appreciated







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OfflineCulliganman360
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Re: Recognizing and dealing with contamination [Re: anunnaki]
    #27797763 - 05/29/22 03:23 PM (2 months, 20 days ago)

Looks sus but usually all germ plates look sus. You can take a transfer from the leading edge now
Or give it a few more days to grow out .
I’ve noticed if I let germ plates sit for awhile they either throw some ryzo growth or start pinning


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OnlineBigworm
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Re: Recognizing and dealing with contamination [Re: anunnaki]
    #27798182 - 05/29/22 08:43 PM (2 months, 20 days ago)

I'm new to the whole agar thing so feel free to ignore me, but IME I would make some transfers now from the 5-8 o clock areas on the first pic. Just the leading edge of the growth. Make a few plates from those sections so if one is contaminated you have back up plates. If you have extra plates take one or two samples from the opposite side as an experiment just to see what happens.


Edited by Bigworm (05/29/22 08:43 PM)


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OnlineBigworm
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Re: Recognizing and dealing with contamination [Re: Aus404]
    #27798193 - 05/29/22 08:51 PM (2 months, 20 days ago)

I would like to say severe bruising from dryness but it looks like more going on than that. From what my untrained eye can see it looks like it's screwed. If it's powdery get it out of the house ASAP as you don't want the spores to spread.


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OfflineBeardedAngler
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27798222 - 05/29/22 09:25 PM (2 months, 20 days ago)

This is my first time using agar and have managed to produce something that I cannot find photos similar too.  Plate was inoculated from a syringe, even though my drop turned into a bit of a squirt.  This plate is 6 days old, and my other plates have not shown any growth yet.  There are small dots around the edge that look correct to me, but I am unsure about the rest.  It almost looks more like a LC on the agar.  Could this be from excess solution, or is it contamination?  Any help or experience is appreciated. 



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Invisiblemilkboy
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Re: Recognizing and dealing with contamination [Re: BeardedAngler]
    #27798242 - 05/29/22 09:41 PM (2 months, 20 days ago)

That is bacteria


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OfflineBeardedAngler
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Re: Recognizing and dealing with contamination [Re: milkboy]
    #27798247 - 05/29/22 09:44 PM (2 months, 20 days ago)

Thanks, I had a feeling it was as it was not uniform, but had not seen any similar photos to verify it


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Invisiblemilkboy
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Re: Recognizing and dealing with contamination [Re: BeardedAngler]
    #27798257 - 05/29/22 09:51 PM (2 months, 20 days ago)

Heres a pic from when I first started out, mycelium, mold, and bacteria all on one plate.


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OfflineBeardedAngler
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Re: Recognizing and dealing with contamination [Re: milkboy]
    #27798264 - 05/29/22 09:59 PM (2 months, 20 days ago)

That is helpful.  I have done cakes, but it has been like 15 years ago.  I knew it was off, but wanted to double check with those more experienced.


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Invisiblemilkboy
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Re: Recognizing and dealing with contamination [Re: BeardedAngler]
    #27798275 - 05/29/22 10:13 PM (2 months, 20 days ago)

Agar is the shit, youll get an eye for it in no time. Becomes an obsession all of its own.


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OfflineAus404
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27798311 - 05/29/22 10:41 PM (2 months, 20 days ago)

The "bruising"  itself isn't powdery and doesn't come off with a Qtip. But the caps have a wet feeling to them with a white substance, which I did accidentally over water the block for about 4 days before I learned better about wetting the block.

I should toss this out then?:



Edited by Aus404 (05/29/22 10:52 PM)


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OnlineBigworm
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Re: Recognizing and dealing with contamination [Re: Aus404]
    #27798345 - 05/29/22 11:06 PM (2 months, 19 days ago)

If it's not trich, which would be powdery, let it ride and see what happens. The wet feel isn't a big deal. The white caps I've had before didn't get much bigger but I've eaten them without adverse effects. I'm not advising you to eat them just giving my experience. Some of the fruits look ok so you might be able to get a small harvest from it.


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OfflineAus404
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27800106 - 05/31/22 12:44 PM (2 months, 18 days ago)

Update on it.

They are looking good. Never seen the white slimy stuff though that is on the caps. It wipes right off though. I'll likely wipe them all down before sticking them in the dehydrator. Probably be good for harvesting Thursday.



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OnlineBigworm
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Re: Recognizing and dealing with contamination [Re: Aus404] * 1
    #27800196 - 05/31/22 01:35 PM (2 months, 18 days ago)

At least it wasn't a total loss. Don't forget to get a clone and or a print for future use.


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Offlinesmalltalk_canceled
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Re: Recognizing and dealing with contamination [Re: Bigworm] * 1
    #27800348 - 05/31/22 03:19 PM (2 months, 18 days ago)

Lol a clone/print from that


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OfflineGuerrilla
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Re: Recognizing and dealing with contamination [Re: smalltalk_canceled]
    #27800352 - 05/31/22 03:22 PM (2 months, 18 days ago)

Quote:

smalltalk_canceled said:
Lol a clone/print from that




He tried

:lol: :lol: :lol:
:canthelpbutlaugh:
:bathtub40lol::loldongs:


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OfflineAus404
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Re: Recognizing and dealing with contamination [Re: Guerrilla]
    #27800628 - 05/31/22 06:56 PM (2 months, 18 days ago)

Sorry for posting so much on this.

I'm still unsure if this looks okay or not, being new to this. It's just that it looks nothing like the B+ I grew, with this having that white film on it.

You can see in these pictures the spots where I took a paper towel and wiped the film off (and bruised a couple spots doing so). The last one bruised blue but this Ajax bruises green I suppose? Just trying to make sure I'm safe about everything.

Like I said I believe I overdid it on the misting early on.





EDIT: Judging by these photos online of Ajax, maybe they are fine and this is just a normal look for the strain and that white film substance is normal?:



Edited by Aus404 (05/31/22 07:09 PM)


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Invisiblemilkboy
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Re: Recognizing and dealing with contamination [Re: Aus404] * 1
    #27800654 - 05/31/22 07:15 PM (2 months, 18 days ago)

Harvest, dry, eat, they are fine. The white on the cap is from the universal veil.


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Offlinesmalltalk_canceled
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Re: Recognizing and dealing with contamination [Re: milkboy]
    #27800697 - 05/31/22 07:45 PM (2 months, 18 days ago)

One one two, do what you gotta do
You can't fight the green
Shits madlib, obscene
So don't take a print from the green Goblin's cake
It'll lead to death 'n be your final mistake.

Maybe make a swab
  Send it for a sporeprint real tell the mirror you upheld
your end o da deal

*swabs die and people stop using them*

Checkmate, swabs


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