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OfflineElusively
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Re: [STICKY] Recognizing and dealing with contamination [Re: cronicr]
    #26563896 - 03/28/20 06:20 PM (3 years, 9 months ago)

Using pasty’s easy agar tek —> bods easy oat prep tek

I feel like this look good, but they were a little fluffy in the glad mini rounds and still look kinda fluffy.

Anyone have some time to reassure my fragile ego?!  Btw, thank in advance for the abundance of info on this site/community, y’all are Effin amazing!



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Offlinejbgtaa
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Re: [STICKY] Recognizing and dealing with contamination [Re: Elusively]
    #26564432 - 03/29/20 01:47 AM (3 years, 9 months ago)

Does look a lil fluffy but you’re gonna need a week or two more to really tell unless it’s really tams out. How long did that take to grow and how many transfers away from the (I assume) spore plate was the culture you use for inoculation?


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OfflineElusively
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Re: [STICKY] Recognizing and dealing with contamination [Re: jbgtaa]
    #26564749 - 03/29/20 08:17 AM (3 years, 9 months ago)

Thanks for the reply sir.  This is my first time w/ agar and a bulk tek, so I’m just feeling it out, doing sort of a “trial run” to see how my technique is and what kind of learning curve I’m working on.    Just giving you some context before I answer your questions:

-that growth is about 5-6 days after innoc those grain jars.

-I used about 1/10th of a wedge in each jar from what I (very noobishly and very arbitrarily) decided was the healthiest of the 4 agar plates I had.

-I did zero transfers, that’s my first go at agar and like I said, I arbitrarily decided that one looked healthy so eff it let’s keep rolling, was my thought process.

-I still have the 3 other agar plates, they’re about 3.5 weeks old now and the growth appears to have stopped, could I still do a transfer with those?


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OfflineInthepit
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Re: [STICKY] Recognizing and dealing with contamination [Re: Elusively] * 1
    #26568473 - 03/31/20 05:40 AM (3 years, 9 months ago)

Thank for this awesome reference and helpful place!
I could use some help to correct whatever I did to get this black mold.
My first guess is the from the water I was misting with.
Possibly I could avoid the black mold by misting with bleach?
:mushdance:   
A little history of the tubs; first they were too wet, haven't dialed in the proportions yet.
Then I took the lids and put them sideways, but I believe the tubs got too dry. Then I started misting heavily.
So basically over correction all around, noob style!

This looks like mold. Black Mold? LOL
My question is if I caught it early
would this How to Make 150 ppm Chlorine Solution by c10 to get rid of it.
And I wonder how I got it, there's lots of mold here in the tropics.
No one drinks the tap water here!


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Edited by Inthepit (03/31/20 07:15 AM)


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OfflineBikerfool
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Re: [STICKY] Recognizing and dealing with contamination [Re: Inthepit]
    #26570251 - 04/01/20 12:36 AM (3 years, 9 months ago)

Inthepit, there's no saving that, throw it away ASAP.

If I ever find any contams it goes instantly into the trash. Even a tiny speck of green mold, I'll pick what's there and throw it out. It's not worth "trying to get one more flush." Trace back through your sterile technique and change or eliminate suspect practices.

My #1 piece of advice to a new grower is to get a flow hood.


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OfflineInthepit
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Re: [STICKY] Recognizing and dealing with contamination [Re: Bikerfool]
    #26570390 - 04/01/20 04:48 AM (3 years, 9 months ago)

Quote:

Bikerfool said:
Inthepit, there's no saving that, throw it away ASAP.

If I ever find any contams it goes instantly into the trash. Even a tiny speck of green mold, I'll pick what's there and throw it out. It's not worth "trying to get one more flush." Trace back through your sterile technique and change or eliminate suspect practices.

My #1 piece of advice to a new grower is to get a flow hood.




Thankyou for the reply.
I wonder if I should use bleach mist anyway as this area has lots of mold.
:hatsoff:


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OfflineGalactic_Grape
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Re: [STICKY] Recognizing and dealing with contamination [Re: Inthepit] * 1
    #26570923 - 04/01/20 10:57 AM (3 years, 9 months ago)

This is day 8 of fruiting, no pins yet. Pf classic with wbs spawn / damion 50/50 coir tek. Woke up this morning and saw these fluffy little balls but I cannot tell if these are knots or the start of trich. Separated this tub from the rest. I’ll know if it goes green by tomorrow but looking for opinions / might be useful to someone down the road if it turns out to be trich so you can see what it looks like before it goes totally green. Spot in question is in the middle of the tub above the crack.

Crack in the sub is from the bottom of the tub bowing down when I had them stacked and then pressed up flat when I put it on the ground.



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OfflineBikerfool
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Re: [STICKY] Recognizing and dealing with contamination [Re: Inthepit]
    #26571048 - 04/01/20 11:58 AM (3 years, 9 months ago)

Quote:

Inthepit said:
Quote:

Bikerfool said:
Inthepit, there's no saving that, throw it away ASAP.

If I ever find any contams it goes instantly into the trash. Even a tiny speck of green mold, I'll pick what's there and throw it out. It's not worth "trying to get one more flush." Trace back through your sterile technique and change or eliminate suspect practices.

My #1 piece of advice to a new grower is to get a flow hood.




Thankyou for the reply.
I wonder if I should use bleach mist anyway as this area has lots of mold.
:hatsoff:




I wouldn't recommend spraying your grow with bleach even if it is a weak solution. If will kill the mushrooms along with all other types of mold.

Having a dirty/ moldy environment is a disadvantage, but if you have good sterile technique and FAE you'll still be successful.


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Edited by Bikerfool (04/01/20 12:15 PM)


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Offlinejudenfisch
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Re: [STICKY] Recognizing and dealing with contamination [Re: Bikerfool] * 1
    #26576664 - 04/04/20 03:57 AM (3 years, 9 months ago)

hello everyone!

the mycelium on the stem of the tallest mushrooms looks strange to me.

the greenish/ blueish spot i was afraid of first is bruising i think...

what do u think?? is it contaminated there? i am very afraid, would be nice to have another opinion!



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Invisiblemurderlabz
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Re: [STICKY] Recognizing and dealing with contamination [Re: judenfisch] * 1
    #26578245 - 04/04/20 10:18 PM (3 years, 9 months ago)

Quote:

judenfisch said:
hello everyone!

the mycelium on the stem of the tallest mushrooms looks strange to me.

the greenish/ blueish spot i was afraid of first is bruising i think...

what do u think?? is it contaminated there? i am very afraid, would be nice to have another opinion!






Hard to see, I have had deep greenish bruising before that freaked me out. Do you have a "sterile" Q-Tip? Swab it, if it transfer to the Q-Tip it's spores from a mould.


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Offlineartkng
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Re: [STICKY] Recognizing and dealing with contamination [Re: murderlabz] * 1
    #26580457 - 04/05/20 08:45 PM (3 years, 9 months ago)

Lots of great info being shared, much needed for new growers. Thanks for the wisdom!


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OfflineGoombala
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Re: [STICKY] Recognizing and dealing with contamination [Re: artkng]
    #26581542 - 04/06/20 11:52 AM (3 years, 9 months ago)

Hey guys does this look sketchy? It has a smell like bread dough which is the only thing scaring me so far.

Spawned on 3/30


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InvisibleR3dstaffy
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Re: [STICKY] Recognizing and dealing with contamination [Re: Goombala]
    #26582170 - 04/06/20 05:01 PM (3 years, 9 months ago)

.


Edited by R3dstaffy (07/23/20 04:13 PM)


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InvisiblecoAsTal
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Re: [STICKY] Recognizing and dealing with contamination [Re: R3dstaffy]
    #26585900 - 04/08/20 09:04 AM (3 years, 9 months ago)

:fffuck:  :badtrip:  :shiiiiit:  :cannotunsee:  :fuckthisshit:

FML-- I just dropped a quart jar full of sporulating trich in my kitchen, releasing a cloud of goddamn spores into the air-- how fucked am i?


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InvisibleRyeHumor
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Re: [STICKY] Recognizing and dealing with contamination [Re: R3dstaffy] * 1
    #26586892 - 04/08/20 05:19 PM (3 years, 9 months ago)

With the exception of that one black spot in the middle photo I would say you're looking fine.

I just birthed my pf jars a week ago and the initial growth looked about like that 2-3 days after noc.

any ideas as to what that black portion in the middle jar is?

If you can get in closer on it, might help.

I was in a similar situation with my jars. I obsessed over it, but really unless you are in a hurry to reuse the jar there's not much you can do other than watch it colonize and see if it expresses obvious signs of contamination.


Edited by RyeHumor (07/26/20 11:00 PM)


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InvisibleRyeHumor
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Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
    #26586902 - 04/08/20 05:24 PM (3 years, 9 months ago)

Quote:

coAsTal said:
:fffuck:  :badtrip:  :shiiiiit:  :cannotunsee:  :fuckthisshit:

FML-- I just dropped a quart jar full of sporulating trich in my kitchen, releasing a cloud of goddamn spores into the air-- how fucked am i?




Might want to wipe down the area/vacuum, especially if you have a hepa filter in your unit. I don't think there's much more you can do.

I was just reading a thread with some input from Bhod and Mad that seemed on a similar subject (cleaning contaminated tubs).

It seems the best thing you can do regardless is just to make damn sure that your spawn is clean AF.

For your future projects, as long as your spawn is fully colonized and contam free, the rest of the process is not sterile and the myc should be strong enough to resist contamination at that point.

So despair not :wink:

I've heard a lot about bleach bombing places, from what I've gathered on this site from more experienced posters is that this is really not necessary. Probably more like soapy water washdown and vacuuming.


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InvisibleR3dstaffy
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Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
    #26587300 - 04/08/20 08:38 PM (3 years, 9 months ago)

.


Edited by R3dstaffy (07/23/20 04:14 PM)


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Offlineonoff
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Re: [STICKY] Recognizing and dealing with contamination [Re: R3dstaffy]
    #26588833 - 04/09/20 01:07 PM (3 years, 9 months ago)

Thanks for the information everyone :smile:
Another first-timer here.
Do you think this may be in vitro pinning or metabolites?
Not sure whether I should fruit these or not, any opinions welcomed!
Thanks.



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InvisiblecoAsTal
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Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
    #26589036 - 04/09/20 02:56 PM (3 years, 9 months ago)

Thank you for the kind words- - I let the area settle for a couple hours then sprayed/misted the area with cleaner, let is get wet, then cleaned it up.
I've already had two months of contamination issues, which is why I was so bummed by this, so maybe in my old age I'm no longer able to properly make clean spawn and am destined to swear off mushrooms forever :fishsmack:

Onward and upward!


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OfflineDezGWest7
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Re: [STICKY] Recognizing and dealing with contamination [Re: onoff]
    #26589537 - 04/09/20 07:26 PM (3 years, 9 months ago)

What's up guys. First timer here. I inoculated WBS March 27 in 3 Jars with GT. The thing is, i see NO activity in 2 of them and the one that I do see activity, I believe its probably contaminated but I'm not so certain. Here is a pic of it. I left it alone originally for the 1st 9 days or so until i decided to check it out and saw what the picture shows but smaller and yes, growth in those 2 different spots. I'm not sure where I f'ed up but *note- this WBS preparation did not include coffee grounds or gypsum. I rinsed , picked the floating grain off, rinsed until clear - soaked 20+ hours - simmered stovetop - then strained , spread out and left to dry a little over 2 hours(I recall it wasn't 100% dry when I decided to pack in the Jar for PS 120min Sterilization. Was it wet wet ? Noo. It just had enough moisture for me to notice when the grains were touching the inside of the jar after loading up. After the PC I allowed it to cool down more than 16 hours from my recollection & then I did the whole sterilize GT syringe etc inoculation of jars.





Feedback is appreciated.


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