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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: LaLaG] * 1
    #27788810 - 05/22/22 10:01 PM (1 year, 8 months ago)

It doesn't look "normal, but I can't say for sure that it is bad. Sometimes you get weird fluffy patches or blobs or other growths. Keep an eye on it but it's likely you'll see fruits soon.


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #27820084 - 06/14/22 10:12 PM (1 year, 7 months ago)

All I see is bruising, a little bit of metabolite stain, and a lot of pins.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: Corey]
    #27822301 - 06/16/22 10:44 AM (1 year, 7 months ago)

Cobweb mold "under control" is as frequent as the common jackelope.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: WyoMX]
    #27830650 - 06/21/22 04:35 PM (1 year, 7 months ago)

Just pop the plastic off the side of the mycelium so the condensation can run down and so it has more surface area to breathe. You can still get fruits from that although it is less than perfect.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: Hindsight] * 1
    #27866950 - 07/17/22 10:53 PM (1 year, 6 months ago)

Quote:

Rotnpins said:
I have no idea what the hell is growing in here...

This was yesterday:



Obviously contam, but I decided to keep it around and see what it did... and less than 24 hours later:




***yes, the agar is slanted :elmo: elmo made me do it :shrug:





That looks like rhizopus.


Quote:

Hindsight said:
This is the first time I have seen this in my LC, though I do VERY little LC so am not that skilled in it.



I am going to guess this is some sort of mold (the bright white growth) like penicillin or trich or something? When I dropped the tiny agar bit into the jar in front of my flow hood, it sank (50/50 some sink some float). The mycelium grew as normal from the bottom of the jar and suddenly one day I noticed this little floater here growing. It floated on the surface for 5 or so days before sinking and now living below the surface.




That one is a toss. :hugitout:


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: Psychonauticsgod21]
    #27941550 - 09/09/22 06:55 AM (1 year, 4 months ago)

How did you work the culture? Was it from spore or a clean looking culture on agar?

Your surface is dry and that might just be matting or funny growth due to that. Those funny looking corners look to me like where the most of the drying airflow is making contact. Give her a mist and see what happens.  The mist will bring out whatever results that tub is destined for.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: Southernlatitude] * 3
    #27967716 - 09/25/22 03:05 PM (1 year, 4 months ago)

Quote:

Southernlatitude said:
I have 5 PF cakes of Golden Teacher on 2nd flush using BRF. Overnight I had multiple turn blue. This is my first go at it. First flush was great. I’m about to toss everything but thought I’d ask first. I thought the blue on the cakes was bruising from harvesting 5 days ago, but I’m a noob and don’t want to ignore possible issues.
The only thing I saw here that looked like it may be this is Penicillium? 
Guidance from a guru is much appreciated!











Classic case of fungus mites.



--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: Guerrilla] * 2
    #27967723 - 09/25/22 03:08 PM (1 year, 4 months ago)

I've seen a few different species now. I clone a lot of weird wild stuff though.

Fungus mites are found everywhere humans dwell including orbital craft. Supposedly they are quite the problem for NASA...

Those are pictures I took with my microscope. You can see all their eggs bound up in the mycelium that's still left.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


Edited by Nichrome (09/25/22 03:10 PM)


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: Rotnpins] * 3
    #27967726 - 09/25/22 03:12 PM (1 year, 4 months ago)

Most people don't even notice it's happening, and it's a lot more common than you think.

Beating your head against the wall trying to figure out why the trich or bacteria shows up no matter how clean your cultures are???  Likely mites, or nematodes. This is a life planet.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: MentalExploder]
    #27968993 - 09/26/22 01:25 PM (1 year, 3 months ago)

From my understanding dehydrated mites are non toxic. If they are not dehydrated at a high enough temp the mites may continue to eat the dried fruit bodies till they are just frass.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: Trabin2836]
    #27987045 - 10/07/22 07:49 PM (1 year, 3 months ago)

Quote:

Trabin2836 said:
https://This is my first grow and it seems to have stalled a bit.  The mycelium grew on rye and I mixed with a substrate of coco coir.  A white fluffy substance grew pretty quickly shown in the pictures.  It has stayed similar to this for about two weeks.  I am fine with patiently waiting this out, but if there is some contamination here I would like to know.





:hmm:


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: larksky] * 1
    #27988784 - 10/08/22 05:31 PM (1 year, 3 months ago)

Quote:

larksky said:
Swab (PESH) to agar 6 days ago. Any input on this one? Swabbed and then dunked the swab in agar.
Anything looking suspicious?







Further transfers will allow what's there to really take form. None of that is mold.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: Bigworm] * 2
    #27988795 - 10/08/22 05:34 PM (1 year, 3 months ago)

A lot of people do.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: milkboy] * 1
    #27989733 - 10/09/22 08:42 AM (1 year, 3 months ago)

:whathesaid:


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Registered: 12/17/18
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Re: Recognizing and dealing with contamination [Re: Bigworm] * 1
    #27991738 - 10/10/22 10:58 AM (1 year, 3 months ago)

There are two completely different cubes that don't get along that are fighting each other in that tub. Not saying there's not mold too but cubes can contaminate cubes too.

Can't we all just get along?


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Registered: 12/17/18
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Re: Recognizing and dealing with contamination [Re: echochild]
    #27992124 - 10/10/22 02:27 PM (1 year, 3 months ago)

Quote:

echochild said:
Quote:

Nichrome said:
There are two completely different cubes that don't get along that are fighting each other in that tub. Not saying there's not mold too but cubes can contaminate cubes too.

Can't we all just get along?




Interesting! That happens even with it all being the same strain, from the same grain bag?





If it was a MS culture then yes it's possible. It's all pretty sus if you ask me. A lot of atypical behavior, materials, and look about that whole tub.

How was the spawn noc'd and what with? What's the long story behind that tub???


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: echochild] * 1
    #27993523 - 10/11/22 10:36 AM (1 year, 3 months ago)

Quote:

echochild said:
Quote:

Rotnpins said:
Check out the first link in my signature.. might help point you in the right direction for your next grow :super:





I have, I've read your sig :smile: I just hate wasting materials I already stupidly ordered. And I figured that if things get messed up, it's education from failure.





If you continue in this venture, you are going to "waste" a lot of materials. It will help your long game to not let it affect you personally.

You waste less materials and time in the long run if you can recognize when something is a loss.  Materials can be replaced. Time cannot be replaced.

Just being blunt here, don't put spores on grain. Don't rely on something being good if it was purchased. People who sell materials have a bottom line and it's rare that quality care is put into producing commercialized materials.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


Edited by Nichrome (10/11/22 10:38 AM)


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: echochild] * 1
    #27995449 - 10/12/22 02:59 PM (1 year, 3 months ago)

I forget which thread I was supposed to post this but regarding orientation of plates in front of the filter and cleaning up cultures, here are a couple examples of before and after.





The Playa/Fiji F3 had mold germinate with the PFi spores. Very easy cleanup. It will/should look clean with the first transfer but will go one more at least before grain.

The other culture is ascomycota with basidiomycota coming in hot.


This is a culture that fluoresces green under uv light. It has a very buggery bacteria riding right along with the mycelium. The bacteria doesn't seem to follow anywhere there is no agar, meaning it doesn't "climb" up the aerial mycelium from what I can see under the microscope so I poured a dribble of agar on a dish lid and replaced the lid on the plate with the culture with the lid with a little agar on it. Pretty sloppy but it's not a top priority. Much care can be applied to this type of rescue attempt should someone have a deep sort of love for their culture they are trying to save.




--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: SymPlayTon] * 2
    #27995539 - 10/12/22 03:55 PM (1 year, 3 months ago)

It is both mold and mycelium. Mold is mycelium.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: Recognizing and dealing with contamination [Re: SirPsycho] * 2
    #27995626 - 10/12/22 05:10 PM (1 year, 3 months ago)

This is one of the most frequently misused terms on this site and amongst the OMC in general.

:mindblown:


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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