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InvisibleLadysKnight
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Re: Is this cobweb or am I just another paranoid N00B? [Re: LittleBoard]
    #27357267 - 06/21/21 07:56 AM (2 years, 7 months ago)

That's mold. Likely was hiding on the agar plate. Remove the LC from your procedure until you have everything else down. Got pics of the plate?


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InvisibleLadysKnight
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Re: Is this cobweb or am I just another paranoid N00B? [Re: LittleBoard]
    #27358517 - 06/22/21 06:48 AM (2 years, 7 months ago)

Not super distinct on the overgrown plate, but see the random white dusty look to the edges, sorta disjointed from the rest of the culture. Yep pretty much the same color too.


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InvisibleLadysKnight
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Re: Is this cobweb or am I just another paranoid N00B? [Re: LittleBoard]
    #27358781 - 06/22/21 12:00 PM (2 years, 7 months ago)

Ok, to simplify, you generally can't see the agar through tomentose mycelium. Mold is thinner, more spotty, more translucent.

Agar to grain may be slower than LC to grain, but not nearly as slow as contaminated LC to grain.


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InvisibleLadysKnight
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Re: Trich or another mold? [Re: gone-pear-shaped]
    #27363136 - 06/25/21 05:30 PM (2 years, 6 months ago)

Has issues yes, but not trich.
See what happens.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: ShamanicRevival]
    #27375004 - 07/05/21 12:15 PM (2 years, 6 months ago)

That tub looks dry as fuck. Even the mold is struggling. I'd take that outside and water it.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: bagga]
    #27375366 - 07/05/21 05:20 PM (2 years, 6 months ago)

Quote:

bagga said:
Just finished second flush - a several pins on one end of the tub have weird orange mycelium.

It's fruited well and has so far been clean, just wondering what this is and if I should be concerned.



Another example


Thanks!



Very bacterial with a little mold. Surprised you got 2 flushes. Again, I put contaminated subs outside for best results.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: tututotutut]
    #27375371 - 07/05/21 05:23 PM (2 years, 6 months ago)

Quote:

tututotutut said:
Hello all, pardon if not the best place to ask:
i am also having an agar problem similar to ranch's.
I have plates that have these very small hardly visible blotches on the surface away from the transfer piece/new myce growth. Its hard to get a picture of. Im under the impression it's bacteria, but i cant say if its from poor non-sterile dishes, or if its poor SAB cleanliness.
And like ranch said, are the dishes trash? Transfer away from the blotches?
It's taking me a very long time to establish +50% agar success :frown:(
Heres an attempt at a photo of suspected dish with blotches

Im hoping its just the way the agar dried... But probably bacteria right




I can't make out anything in the first pic, too overgrown, but the second pic does look like the outline of bacteria.

Generally, you don't want to wait this long. After you see clean growth, you want to transfer the best piece to a new plate.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: DERRAYLD] * 1
    #27380182 - 07/09/21 07:46 AM (2 years, 6 months ago)

Idk, those serrated edges might keep me focused. :lol:


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: LittleBoard]
    #27381464 - 07/10/21 06:26 AM (2 years, 6 months ago)

Quote:

LittleBoard said:

I have another question: I recently had moldy spawn but one shoebox did well and flushed normally. So I cloned from one shroom in there.
Is there mold inside the mushroom, or will the mycelium fight this and not let anything it inside the fruit?
The idea was that I could throw away all the agar plates that lead to the moldy spawn but that I could save the genetic by cloning the survivors.
The plate that I made from them shows good, fast rizomorphic growth as far as I can tell.




Since the mold came from the spawn, it's likely enmeshed. Got plate pics?


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: gone-pear-shaped]
    #27383867 - 07/12/21 03:57 AM (2 years, 6 months ago)

I try to keep any vibration to a minimum. It's fine to use the rim of the jar to release the agar, only the agar wedge and the tip of the blade should pass the rim.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: gone-pear-shaped]
    #27383993 - 07/12/21 07:17 AM (2 years, 6 months ago)

I use one wedge per jar. I inoculate 10 jars with one 100mm plate. I haven't seen enough of an improvement to use more than that. Also my PC does 10 quarts at a time. I only have to deal with one donor plate so less in and out, etc.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: Nonagon] * 2
    #28171381 - 02/03/23 10:05 PM (11 months, 17 days ago)

Quote:

Nonagon said:
Quote:

Nonagon said:
Some follow up pics from my weird enmeshed mold plates. Rolled the dice and lost on this one :lol: Interesting seeing the mycelium go all crazy where it meets the contam though


Only leaves the footprint of the myc growth once it goes to grain, any further thoughts as to why this is?






Agar pics for fluffy/sketchy plate reference






I have a couple prints contaminated with this, I've tried for a year off and on to get away from it. I call it a parasitic white mold. It causes super slow growth at all stages and mostly aborts in a tub. Even plate pins and clone tissue has it. For me, I can see it as a clear, shiny halo that can be seen at the margins of germs and transfers with a bright light at just the right angle. Once the plate grows out it's invisible. This is why I can't fathom using any agar dish that's not perfectly clear.


Edited by LadysKnight (02/03/23 10:15 PM)


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: Nonagon]
    #28171731 - 02/04/23 07:38 AM (11 months, 17 days ago)

For me, it doesn't show normal mold-sign. No visible sporulation, no fast aggressive growth, colors, etc. Besides on a petri dish, I can never point at it and say, that's mold. But I see it in 90% aborts, everything taking 4x longer to colonize.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: AyePlus] * 1
    #28171746 - 02/04/23 07:53 AM (11 months, 17 days ago)

Agreed, I need a scope.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: AyePlus]
    #28171766 - 02/04/23 08:13 AM (11 months, 16 days ago)

Will do, thanks bro


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: AyePlus]
    #28176373 - 02/07/23 02:49 PM (11 months, 13 days ago)

Quote:

AyePlus said:
I’m also familiar with the same phenomenon as ladysknight. Way back in the day bod told me it was just bad bacteria but I wasnt convinced and also had a plate in cold storage grow out a funny halo




I’m under the impression its a mold that parasitizes mushrooms and sporulates on them, so a fruit swabbed or printed from an affected tub would have it too



Quote:

LadysKnight said:
Quote:

Nonagon said:
Quote:

Nonagon said:
Some follow up pics from my weird enmeshed mold plates. Rolled the dice and lost on this one :lol: Interesting seeing the mycelium go all crazy where it meets the contam though


Only leaves the footprint of the myc growth once it goes to grain, any further thoughts as to why this is?






Agar pics for fluffy/sketchy plate reference






I have a couple prints contaminated with this, I've tried for a year off and on to get away from it. I call it a parasitic white mold. It causes super slow growth at all stages and mostly aborts in a tub. Even plate pins and clone tissue has it. For me, I can see it as a clear, shiny halo that can be seen at the margins of germs and transfers with a bright light at just the right angle. Once the plate grows out it's invisible. This is why I can't fathom using any agar dish that's not perfectly clear.






I think this is some different kind of parasite similarly spread, which would explain a clean clone. Run that and print it and give up on this print IMO,

It might be a trichoderma species considering the enmeshed nsture, late appearance and green but hard to say. Would be interested to see it under a scope



Edit: just had the thought that since its running faster than the cube once it gets to a more nutrituios media (grain) maybe taking it to a lower or even no nutrient media like water agar would give the cube myc a chance to outpace it.




Yo bro, I'm glad you chimed in on this one, I've been dealing with this for years and was beginning to feel like the lone ranger. Doubting myself and everything I've been seeing. A lot of well respected cults and TCs seem unfamiliar and told me it was normal. Now I'm a new man. :smile:


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: PBJ710] * 1
    #28186899 - 02/14/23 09:36 AM (11 months, 6 days ago)

Since you expect contamination, top fruit those jars after you take a grain to agar. See what you get. Likely will fruit. Then try taking a clone to agar.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: MycoSnailCarl]
    #28572466 - 12/07/23 07:21 AM (1 month, 21 days ago)

Quote:

MycoSnailCarl said:
Quote:

Zwinst said:
You already lost it. Trying to cut it out wont work, it will come back in 2 days or so.

If you are desperate harvest the couple of big ones, but do discard the rest before everything turns green.

edit: Very nice looking shrooms btw. What are they?




Any reason for him not to harvest the smaller ones as well? Will they not have developed actives yet?




All are good to pick


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: MycoSnailCarl]
    #28572469 - 12/07/23 07:25 AM (1 month, 21 days ago)

:cheers:


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: WyoMX] * 2
    #28591113 - 12/20/23 09:49 AM (1 month, 7 days ago)

Looks more like the PC run didn't work. The mold dispersement is too even to be lid or inoculum related


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