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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose, Pressure Cooker, Wild Bird Seed

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Offline95rh
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Registered: 05/03/03
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agar grams ounces suggestions
    #2309409 - 02/06/04 07:08 PM (13 years, 26 days ago)


Hiya Folk

OK. so we got some Malt Extract agar from fungi perfecti it says to mix 3/4 of an ounce agar to one liter of water.

My math skills are minimal and my bud only has 12 plates to play with ie: one shot with a liter of agar mix so he wants to double check and make sure what he's thinking will work actually will.

is 21 grams of powder equal to 3/4 ounce? mix this with 1 liter of purified water pc for 20 min at 15 psi let cool for an hour inside the cooker then pour into petri dishes, let cool overnight then inoculate with sporesyringe. incubate dishes at 80-85 deg F and wait for full growth before cutting into 8 wedges and using these to noc up some qt jars of birdseed or popcorn?

What does this plan sound like to ya'll? Seen a bunch of teks on using agar and figure its close but want to be sure before messing up.

thanks for any and all feedback
rh95

note to self next time eithier get more dishes or pick up some glass ones that can be reused


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InvisibleGoldenShroom
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Registered: 03/13/03
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Re: agar grams ounces suggestions [Re: 95rh]
    #2309654 - 02/06/04 08:16 PM (13 years, 26 days ago)

yes 21 grams sounds right... although the recipe I have used is 20g per 1/2 liter of water. Mine worked fine that way, and I'm sure yours will too since that's what your directions say.


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Offline95rh
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Re: agar grams ounces suggestions [Re: GoldenShroom]
    #2312923 - 02/07/04 10:45 PM (13 years, 25 days ago)


20g per 1/2 liter? thats twice what the bottle says weird. guess the only way to find out for sure is to just do it and hope it works.

rh95


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Offlinellamaboy
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Re: agar grams ounces suggestions [Re: 95rh]
    #2313074 - 02/08/04 12:00 AM (13 years, 25 days ago)

my recipe also calls for ~20 grams per .5 litre. but i use PDA, maybe there's a difference.


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OfflineAnnoA
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Re: agar grams ounces suggestions [Re: 95rh]
    #2313424 - 02/08/04 02:37 AM (13 years, 25 days ago)

A standard MEA recipe calls for 20 g agar and 20 g malt extract per 1 liter.
So this would be 40 grams of the mixture per liter.

Are you sure you have bought a mixture, or do you have agar only?


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OfflineDSD
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Re: agar grams ounces suggestions [Re: Anno]
    #2313567 - 02/08/04 03:53 AM (13 years, 25 days ago)


directions ?  :blush:


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OfflineDSD
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Re: agar grams ounces suggestions [Re: Anno]
    #2313576 - 02/08/04 03:57 AM (13 years, 25 days ago)


very beautifull woman anno !


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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose, Pressure Cooker, Wild Bird Seed

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