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Offlineabcubensis
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1st time PFtek method. UPDATE/EDIT - Spawned to bulk sub monotub, cased, questions about pinning.
    #22944909 - 02/25/16 01:15 PM (7 years, 11 months ago)

***SEE COMMENT BELOW FOR UPDATE***

So I left my original post in italics below. To update, I basically have 6 fully colonized B+ PFtek jars 1 month after inoc.

I have taken the jars out of incubator into kitchen, where they receive 10ish hours of indirect light around 70°F. I have not removed the cakes from the jars yet.

On day 2 after removing from incubator, I wasn't seeing any results of pinning or primordia. So I put jar in sealed bag in fridge over night. Removed from fridge after 12 hours and have been sitting on counter since.

Now, day 5, (3 days after fridge), still not seeing any Primordia or pinning. I plan to case them - which I am researching now. It seems there are a million ways to fruit them.

Do I need to see any Primordia or pinning (same thing right?) before removing them from jars? Especially if I plan to case them?

Should I keep them in fridge longer to initiate pinning? Should I be doing anything differently?

I'm sure this all has been asked before, but I can't find any great specific info.


One other note: a few of the cakes appear to have shrunk inside the jars, not all of them though.


Hey all,
First time poster. I needed a project to keep me busy over the winter (I freelance, winter months are slow). So i decided to try my hand at growing. I picked up two syringes of B+ from a trusted seller and have am currently going through the PFtek method. I've learned a lot in the process and realize some of the mistakes.

I inoculated a total of 16 jars (8 jars per syringe). A total of 6 jars have begun to colonize 3 weeks later. There are probably too many variables to definitively say what went wrong, but wondering if anyone has any input.

I followed the PFTek method pretty closely. They have been colonizing for 3 weeks in my homemade incubator at about 80 degrees. Here are the mistakes or moments of uncertainty I had:

-Used FINE grade vermiculite. Didn't realize the importance of this until after inoculation. I am afraid that there isn't enough air in some of the jars.

-For the second batch, I used taller jars. However, I mixed everything basically the same and used the same amount of substrate for the jars. The batch of tall jars only has 1 or 2 jars colonizing.

-I didn't really record the amount of water used in substrate. I used mineral water from a bottle. I have a feeling this might be where I went wrong. I used enough water to get the verm sopping, then drained all the excess water pretty well.

-Could the sterilization process have dried out the jars? I don't have a pressure cooker, so I just boiled the jars in an inch or so of water for 90 minutes. I doubt this is the case as probably none of the jars would have colonized if sterilizing had dried the jars.

Any ideas as to why most of the jars haven't colonized? I just find it odd that 6 of them have and I basically used the same process for each jar.

Note on pics, these are just a couple random jars I pulled. Some show a little condensation on the inside of the jars (both colonized and not colonized). If this helps.



Edited by abcubensis (04/01/16 12:33 PM)


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OfflineMorel Guy
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Re: 1st time PFtek method. More than half of my jars haven't started colonizing after 3 weeks. [Re: abcubensis]
    #22945322 - 02/25/16 02:47 PM (7 years, 11 months ago)

First pic looks great.  80 is a bit high for incubating.  Field capacity is what you want your substrate to be.  Field capacity is when you must squeeze the medium with your hand to get a few drops of water.

Did you flame the needle in between holes and jars?  I like to drip the spores along the inside wall of the jar.  If your concerned you could open a jar for a sniff.


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Offlineabcubensis
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Re: 1st time PFtek method. More than half of my jars haven't started colonizing after 3 weeks. [Re: Morel Guy]
    #22954962 - 02/28/16 12:01 PM (7 years, 11 months ago)

I previously responded, not sure why it didn't appear. But to answer your question, yes, I flamed the needle between inoculations and I did drip the spores along the walls of the jar.

Any last-ditch recommendations to get the remaining jars to colonize?


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OfflineGroo
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Re: 1st time PFtek method. More than half of my jars haven't started colonizing after 3 weeks. [Re: abcubensis]
    #22955089 - 02/28/16 01:00 PM (7 years, 11 months ago)

Trusted source obviously was not viable enough to work pf method. Which is poor quality and I am sure they tired their best and he's still a nice guy. Now unless you wanna start learning agar. Start with sponsor spores and put THOSE under your microscope.. I suggest you check out our sponsors if you want the next level. 


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OfflineLogicaL ChaosM
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Re: 1st time PFtek method. More than half of my jars haven't started colonizing after 3 weeks. [Re: abcubensis]
    #22955206 - 02/28/16 01:47 PM (7 years, 11 months ago)

I think u used too much water when u made your cake substrate. They look overly wet.

I would take that foil off and leave the stalled jars in a dry area with lots of sunlight. Might help dry them out without opening the jar lid.


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OfflineGroo
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Re: 1st time PFtek method. More than half of my jars haven't started colonizing after 3 weeks. [Re: LogicaL Chaos]
    #22955217 - 02/28/16 01:50 PM (7 years, 11 months ago)

Really really good idea! I noticed that earlier and then was like fuck it says he followed it to a T ill give him the spheel about buying new spores.

But that was true brilliance and the best possible last ditch But yeah after that new sprs. or Agar. one or the other.


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Offlineabcubensis
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Re: 1st time PFtek method. More than half of my jars haven't started colonizing after 3 weeks. [Re: Groo]
    #22992498 - 03/10/16 12:56 PM (7 years, 11 months ago)

Just updated my thread. Also, I'll try drying out the ones that haven't colonized.

Groo - I think the spores came from one of the Sponsors. I don't think you're allowed to post the name of the sponsor in threads. Of all the vendors this one seemed most popular. Where is the list of sponsors?

This noob will have to look up what "Agar" even means. I'm learning a lot but it's really fun.


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OfflineLogicaL ChaosM
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Re: 1st time PFtek method. More than half of my jars haven't started colonizing after 3 weeks. [Re: abcubensis]
    #22993027 - 03/10/16 03:37 PM (7 years, 11 months ago)

Agar is the secret to potent, consistent, highly-AWESOME grows!


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Offlineabcubensis
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Re: 1st time PFtek method. UPDATED: Spawned to cased hpoo bulk in monotub, more questions!! [Re: LogicaL Chaos]
    #23071417 - 04/01/16 01:14 PM (7 years, 10 months ago)

So everything went pretty well, I ended up spawning to a bulk substrate. Here is a loose recipe I used for the substrate:

-hpoo (leached, was already mixed with straw)
-coarse verm
-coco coir
-pickling lime (for ph)
-H20 until field capacity

I pasteurized the substrate. Created the monotub, sealed up the holes for colonizing, lined the tub with a black trashbag, and filled with about 2.5-3" of substrate. (Keeping everything as sterile and clean as possible).

I then crumbled 8 or 9 fully colonized B+ BRF cakes and mixed into the substrate. Covered with tin foil. Sealed off from light and kept it at roughly 80°F.

The substrate surface was colonized in 3 days, let it go for another 3 or 4 to colonize the underlying layer.

I realize the debate about the need to case cubensis, however I decided to do it. I made the casing mix using roughly 48% Jiffy Mix, 48% coarse verm, 4% Gypsum and pickling lime for Ph of around 7.5. H20 to field capacity.

During stovetop pasteurization, in retrospect, I think some water may have gotten into bag because the casing mix seemed a little wetter after pasteurization. I unfortunately didn't pay enough attention at the time as it seemed to be field capacity before pasteurization.

Right before adding casing layer, I noticed there were a couple drops of water collected atop the substrate, not being absorbed. Does that mean overlay? Is that a problem if I put casing over that?

I added about 1/2" of the casing layer, and put the tub back into colonizing conditions: sealed from light 24hrs, and at about 80°F. I don't know if this was right or not. I read that you want to let the casing layer colonize a little before bringing the tub into fruiting conditions, but later read you don't want to do this because of overlay. I lightly patched the first aggressive mycelium poking through.

Anyways, the casing layer colonized to about 80%. Two days ago I brought the tub into fruiting conditions. I dropped the temp to 70°F, unsealed the holes (replacing gaff tape with polyfill and micropore tape). I have been adding light for about 12 hrs. I have also been fanning out twice a day, for good measure.

FINALLY, the point of my post:
It has been in fruiting conditions for two days and I have not seen any evidence of primordials forming. It looks the same as it did two days ago.

As stated before, I fear the casing layer may be too wet. I haven't misted at all and even with the fanning, there is constantly a layer of condensation in the tub.

Other fears:
Did I allow the casing layer to colonize too much?
From the pics, do you guys see any evidence of overlay?

Any recommendations? When should I expect to start seeing Primordia or shroomies growing?

Thanks for the help!

These pics are what it looks like now. After casing, two days into fruiting conditions. Also, the temp and moisture readings are a little low due to just being fanned and placed near cracked window.:



Edited by abcubensis (04/01/16 06:17 PM)


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Offlinetwistedty
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Re: 1st time PFtek method. UPDATED: Spawned to cased hpoo bulk in monotub, more questions!! [Re: abcubensis]
    #23071758 - 04/01/16 02:49 PM (7 years, 10 months ago)

you need some holes at sub level.  looks good other wise.  tape up the top holes on the side and drill em down an inch or two above substrate


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Offlineabcubensis
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Re: 1st time PFtek method. UPDATED: Spawned to cased hpoo bulk in monotub, more questions!! [Re: twistedty]
    #23072476 - 04/01/16 06:19 PM (7 years, 10 months ago)

I didn't realize this. I thought they were supposed to be an inch above substrate. Thanks for the tip.


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