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GreyThumb
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Liquid Culture Help for a newbie
#23031247 - 03/21/16 07:36 PM (8 years, 9 months ago) |
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Hey all!
I read this FAQ (https://www.shroomery.org/62/Liquid-Growth-Medium) it says spore syringe so that's what I used, but then I read on the forums you aren't suppose to use a spore syringe?
I'm trying to figure out is this stuff is mycelium, looks like it to me! I'm guessing the black pieces are spores, is that right? Thanks..
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Re: Liquid Culture Help for a newbie [Re: GreyThumb]
#23031410 - 03/21/16 08:17 PM (8 years, 9 months ago) |
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Quote:
GreyThumb said: Hey all!
I read this FAQ (https://www.shroomery.org/62/Liquid-Growth-Medium) it says spore syringe so that's what I used, but then I read on the forums you aren't suppose to use a spore syringe?
I'm trying to figure out is this stuff is mycelium, looks like it to me! I'm guessing the black pieces are spores, is that right? Thanks..

Could be, won't know until you test it... Since spores in general aren't clean, spores to LC is not a good idea... You wanna go spores>agar>Lc, so you can make sure your culture is clean... Contams are hard af to see in an Lc
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bw86
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Re: Liquid Culture Help for a newbie [Re: LocN9ne]
#23031434 - 03/21/16 08:23 PM (8 years, 9 months ago) |
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Yes that appears to be mycelium but maybe not cube myc. Could be mold or any number of things.
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GreyThumb
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Re: Liquid Culture Help for a newbie [Re: bw86]
#23031679 - 03/21/16 09:25 PM (8 years, 9 months ago) |
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Thanks for the replies!! Guess I'm in way over my head... lol
Is there an easy way to test it without a lab? Thanks!
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Liquid Culture Help for a newbie [Re: GreyThumb]
#23031717 - 03/21/16 09:38 PM (8 years, 9 months ago) |
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Agar... That's precisely what it is for.
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AIO
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Re: Liquid Culture Help for a newbie [Re: GreyThumb]
#23031724 - 03/21/16 09:39 PM (8 years, 9 months ago) |
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You could try transferring a piece to a test jar and see what happens.
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psilocybeMAN
It gets so real sometimes.



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Re: Liquid Culture Help for a newbie [Re: GreyThumb]
#23031769 - 03/21/16 09:55 PM (8 years, 9 months ago) |
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Yea OP, hopefully the syringe was clean. Would be ideal to start from agar however it is not required. Looks alright to me. Knock up some jars and find out.
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GreyThumb
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Re: Liquid Culture Help for a newbie [Re: psilocybeMAN]
#23032193 - 03/22/16 12:55 AM (8 years, 9 months ago) |
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LocN9ne - I believe you about the agar, it's just new to me so I don't have the stuff for it. I've only done casings before.
So when you guys say jars... you mean agar jars? petri dishes? or rye berry jars?
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psilocybeMAN
It gets so real sometimes.



Registered: 10/16/07
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Re: Liquid Culture Help for a newbie [Re: GreyThumb]
#23032220 - 03/22/16 01:10 AM (8 years, 9 months ago) |
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Quote:
GreyThumb said: So when you guys say jars... you mean agar jars? petri dishes? or rye berry jars?
Watch this video OP.
Edited by psilocybeMAN (03/22/16 01:54 AM)
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Mycologist217
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Re: Liquid Culture Help for a newbie [Re: psilocybeMAN]
#23032250 - 03/22/16 01:22 AM (8 years, 9 months ago) |
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I encourage you to research some more....my signature has plenty of up to date information on liquid culture.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE
Check out my Retail Gourmet Mushroom Farm!
Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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GreyThumb
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Re: Liquid Culture Help for a newbie [Re: GreyThumb]
#23173052 - 04/30/16 02:55 PM (8 years, 8 months ago) |
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It seemed to grow nicely, I put it in the fridge and it froze... lol
Still going to try and use it someday... Frozen mycelium? Can that be salvaged?
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Ogla
losfreddy



Registered: 02/16/04
Posts: 11,500
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Re: Liquid Culture Help for a newbie [Re: GreyThumb]
#23173089 - 04/30/16 03:15 PM (8 years, 8 months ago) |
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I go from syringe to LC all the time and never have much problems. Its not always 100% thou.
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Ogla
losfreddy



Registered: 02/16/04
Posts: 11,500
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Re: Liquid Culture Help for a newbie [Re: GreyThumb]
#23173093 - 04/30/16 03:16 PM (8 years, 8 months ago) |
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Quote:
GreyThumb said: It seemed to grow nicely, I put it in the fridge and it froze... lol
Still going to try and use it someday... Frozen mycelium? Can that be salvaged?
whoah, dont freeze it dude. That'll kill it for sure
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Mad Season
hookers and blackjack



Registered: 09/16/12
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Re: Liquid Culture Help for a newbie [Re: Ogla]
#23173678 - 04/30/16 06:43 PM (8 years, 8 months ago) |
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Quote:
losfreddy said: I go from syringe to LC all the time and never have much problems. Its not always 100% thou.
You're so trusting. I guess I'm just always sceptical of spores. Its not like they can autoclave them to ensure they're clean of contams.
But that's why I advocate spores > agar cleanup > LC > agar testing > MOAR SPAWN
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Ogla
losfreddy



Registered: 02/16/04
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Re: Liquid Culture Help for a newbie [Re: Mad Season]
#23173698 - 04/30/16 06:49 PM (8 years, 8 months ago) |
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yes, that actually precisely what im about to attempt with a wild collected specimen. isolate on Agar, then transfer to LC
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Liquid Culture Help for a newbie [Re: Ogla]
#23173699 - 04/30/16 06:50 PM (8 years, 8 months ago) |
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Jealous. I want a wild print lol.
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GreyThumb
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Re: Liquid Culture Help for a newbie [Re: Mad Season]
#23181349 - 05/02/16 06:38 PM (8 years, 8 months ago) |
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I didn't mean to freeze it I just wanted it in the fridge for a few days... I'm still attempting to thaw it out and use it...
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