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OfflineNickster_154371
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alchohol sterilization for making spore prints?
    #2303015 - 02/05/04 12:48 AM (12 years, 9 months ago)

Im looking to make a few spore prints in a few days using these small 3 1/4 oz plastic little cups. Ive tried microwave sterilizing them by putting some water, wrapping w/ suran wrap, and heating for 5 min., but they melted on me! Would i be fine if i just poured a bit of alchohol in each and rubbed them down, then put the cap in, and place a tight piece of suran wrap w/ a couple holes punched in them?


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OfflineExtravagantDream
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Re: alchohol sterilization for making spore prints? [Re: Nickster_154371]
    #2303109 - 02/05/04 01:16 AM (12 years, 9 months ago)

Yes, it should work, I used alcohol all the time instead of heat. Just make sure the surface gets exposed to the alc long enough before it evaporates, say 30 seconds.


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OfflineNickster_154371
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Re: alchohol sterilization for making spore prints? [Re: ExtravagantDream]
    #2303164 - 02/05/04 01:30 AM (12 years, 9 months ago)

Thanks a ton, you just saved me alot of work  :grin:


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Re: alchohol sterilization for making spore prints? [Re: Nickster_154371]
    #2303278 - 02/05/04 02:01 AM (12 years, 9 months ago)

i just put them on folded(so it isnt flimsy, and more stable) tinfoil, which i wipe with alcohol as well, then i put that into a small tupperware container(also wiped out with alcohol) and put lid on it and done.


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OfflineExtravagantDream
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Re: alchohol sterilization for making spore prints? [Re: Nickster_154371]
    #2304038 - 02/05/04 10:40 AM (12 years, 9 months ago)

ohh and why the holes? Seems like a contam factor to me.


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OfflineTeknion
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Re: alchohol sterilization for making spore prints? [Re: ExtravagantDream]
    #2304133 - 02/05/04 11:18 AM (12 years, 9 months ago)

From what I read, you'd want holes so the caps can dry up and release more spores, instead of sticking to the damp cap.

Though I may be wrong.

Actually, this raises a question in my mind: Instead of putting holes in your container, could you put a little sack of dessicant in there to absorb moisture?

Or both maybe?


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InvisiblePrisoner#1M
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Re: alchohol sterilization for making spore prints? [Re: Teknion]
    #2304694 - 02/05/04 01:54 PM (12 years, 9 months ago)

you dont want the cap to dry too quickly...dont put holes in anything...let the cap sit overnight while printing, sometimes it takes a couple days...if the cap dries out then the best thing to do is place a drop of water on the top of the cap in order to give a little more moisture...


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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