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Invisiblekeyeghost
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Registered: 09/04/02
Posts: 123
Is there anything wrong with this agar recipie please?
    #2302837 - 02/04/04 11:50 PM (13 years, 4 months ago)

This is my friends first attempt using agar. She did the following:

Got 150ml of water from boiled potatoes
added 350ml distilled water
added 10ml honey
added 9.2g AGAR AGAR flakes.

The above were placed in a quart jar she said, and allowed to pc for 27 min @ 15 psi. After the recipie was made, it was allowed to cool to 110F and then 6.5 ml of H202 were added. Before solidifying they were poured in to sterilized 4 oz Ball jars (WAL-MART 4$) over a HEPA filter. Anyone see any problems?
Thanks,

Ki


--------------------
"time couldn't end me, even the great devil Satan who tried to befriend me, understand the equality, God in the bodily form, lettin my knowledge be born" RZA as Prince Rakeem


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Offlineamyloid
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Registered: 03/08/03
Posts: 980
Last seen: 3 years, 4 months
Re: Is there anything wrong with this agar recipie please? [Re: keyeghost]
    #2302890 - 02/05/04 12:08 AM (13 years, 4 months ago)

over a hepa filter? what does that mean? a hepa filter blowy thinger? if so, sounds alright, sounds like alot of agar for those jars though. not that it matters.


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"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Offlinebluhoney
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Re: Is there anything wrong with this agar recipie please? [Re: amyloid]
    #2302904 - 02/05/04 12:12 AM (13 years, 4 months ago)

Sounds good, my friend hasnt tried a honey agar tek yet, do you mind if she tries yours? Amyloid, your right, I think keyeghost used the hepa filter to reduce the bad stuff while she exposed the jars. :smile: bluhoney


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Information listed here is for entertainment only and is neither real or proven


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Invisiblekeyeghost
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Registered: 09/04/02
Posts: 123
Re: Is there anything wrong with this agar recipie please? [Re: bluhoney]
    #2303277 - 02/05/04 02:01 AM (13 years, 4 months ago)

Of course Blu, it is just a slight variation of the one in the FAQ, i definitely can't take credit for it.

BTW, I just report for my friend, she doesn't have a net connection.

In reply to Amyloid: Yes, she said it was done over a HEPA flow hood.

Well I guess I will report back to report how things turn out. BTW, my friend also figured that was a bit too much agar for those jars, but my friend figures longer colonization can't do anything except invite more contams and potentially ruin the whole experiment. But hey, you win some and you lose some! Thanks for the replies!

Ki

BTW can anyone inform me more as to the process of caramelization: Why it is bad, what times of pcing are needed to achieve it? I searched the FAQ, but mostly found mumbo jumbo!

Thanks


--------------------
"time couldn't end me, even the great devil Satan who tried to befriend me, understand the equality, God in the bodily form, lettin my knowledge be born" RZA as Prince Rakeem


Edited by keyeghost (02/05/04 03:50 PM)


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Invisiblethescientist
Registered: 03/10/02
Posts: 788
Loc: Dade County
Re: Is there anything wrong with this agar recipie please? [Re: keyeghost]
    #2305978 - 02/05/04 06:24 PM (13 years, 4 months ago)

i found using potatoes a pain so i use flaked potatoes now, use about 5 g's per litre, works well for all the spp's i grow


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Offlinebluhoney
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Re: Is there anything wrong with this agar recipie please? [Re: keyeghost]
    #2306477 - 02/05/04 09:25 PM (13 years, 4 months ago)

This is straight from "The Mushroom Cultivator" book p.21, ...Sugar in media will caramelize. Media with caramelized sugar inhibits mycelial growth and promotes genetic mutations...:)bluhoney


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Invisiblekeyeghost
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Registered: 09/04/02
Posts: 123
Re: Is there anything wrong with this agar recipie please? [Re: bluhoney]
    #2307025 - 02/05/04 11:55 PM (13 years, 4 months ago)

Hmmm thanks blu ...
My friend has read about honey being used in AGAR recipies, as long as pcing was not too lengthy. So, we will see how it works out and report back!

Thanks

Ki


--------------------
"time couldn't end me, even the great devil Satan who tried to befriend me, understand the equality, God in the bodily form, lettin my knowledge be born" RZA as Prince Rakeem


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