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Invisibleturtlelamp
n00b
Registered: 11/27/03
Posts: 93
Loc: The Midwest
No jars will germinate. Again
    #2300022 - 02/04/04 11:19 AM (12 years, 9 months ago)

Right now I have four sets of jars in my incubator with innoculation dates ranging from the middle of January to three days ago. I don't have any growth in any of them. I waited over night before I innoculated any of them, so I know it isn't that. What could be killing these spores? Is it possible there's something in my city water that is killing them? I've had success before. It's about a ratio of one successful batch to three failures. I'm lost.


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InvisibleMilkVein
unsure
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Registered: 08/27/03
Posts: 2,695
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Re: No jars will germinate. Again [Re: turtlelamp]
    #2300028 - 02/04/04 11:25 AM (12 years, 9 months ago)

check water content..it shouldnt be something in the water....you PC the jars right......theres no contams?! more info


--------------------
I've been first and last
Look at how the time goes past.
But I'm all alone at last.
Rolling home to you.


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Invisibleturtlelamp
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Registered: 11/27/03
Posts: 93
Loc: The Midwest
Re: No jars will germinate. Again [Re: MilkVein]
    #2300038 - 02/04/04 11:30 AM (12 years, 9 months ago)

I'm sure the water content in fine. Three sets are pf-style brf and one set, the newest, is wbs in pint jars. The first two sets were innoculated with syringes purchased from a shroomery vender. The second two were made with spore prints I took personally. I've tried so many different things and yet I can't get anything, not even contams, to grow in these jars. I figure it has to be a factor that's consistent with all the jars. Namely, the water, the air or satan.


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InvisibleTHEBOSS
THE BOSS

Registered: 12/16/03
Posts: 1,252
Re: No jars will germinate. Again [Re: turtlelamp]
    #2300067 - 02/04/04 11:43 AM (12 years, 9 months ago)

What temps are they at?odds are its not the spores if you bought from a shroomery sponsor. Are the lids loose? Whats where your ratios?


THE BOSS


--------------------
Mushbox Forum


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Invisibleturtlelamp
n00b
Registered: 11/27/03
Posts: 93
Loc: The Midwest
Re: No jars will germinate. Again [Re: THEBOSS]
    #2300077 - 02/04/04 11:49 AM (12 years, 9 months ago)

They're in my incubator at 83F. I have a dual-rubbermaid setup with a submerged aquarium heater in the bottom tub. I have the top lid on and a blanket on top of the whole mess. I used 2:1:1 verm:brf:water and soaked the wbs for 9 hours, straining for 1.


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Anonymous

Re: No jars will germinate. Again [Re: turtlelamp]
    #2300081 - 02/04/04 11:50 AM (12 years, 9 months ago)

do you have the jar lids inverted? ...could be the problem

btw, you should soak your birdseed for atleast 24 hours, imo

but no longer than 36...


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OfflineDrWho
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Registered: 01/08/04
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Re: No jars will germinate. Again [Re: ]
    #2300098 - 02/04/04 12:00 PM (12 years, 9 months ago)

should the lids be loose or tight?


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Invisibledobinky
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Re: No jars will germinate. Again [Re: DrWho]
    #2300104 - 02/04/04 12:02 PM (12 years, 9 months ago)

tight at first, but looser as they are about fully colonized to allow more o2 in for the mycelium


--------------------
Today?s Pig is Tomorrow?s Bacon


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OfflineMikeOLogical
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Registered: 01/31/04
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Re: No jars will germinate. Again [Re: turtlelamp]
    #2300129 - 02/04/04 12:15 PM (12 years, 9 months ago)

give the jars a good sniff... what kind of water did you use? If you live somewhere with hard water you'll have big problems with tap water...


--------------------
We got Nothing!
we're no longer selling jars.  :laugh:


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Invisibleturtlelamp
n00b
Registered: 11/27/03
Posts: 93
Loc: The Midwest
Re: No jars will germinate. Again [Re: MikeOLogical]
    #2300183 - 02/04/04 12:30 PM (12 years, 9 months ago)

They're not contaminated, this I know for sure. I am concerned with the water, though. It is tap and it is hard. How can I use anything else though? Does everyone else just buy 10 gallons from the grocery store every week?


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Anonymous

Re: No jars will germinate. Again [Re: turtlelamp]
    #2300199 - 02/04/04 12:34 PM (12 years, 9 months ago)

if its the water, then yes, go buy some.  do what ya gotta do :wink:  otherwise make sure the damn lids are inverted  :lol:  you didnt say if they were or not, some people overlook such a simple thing  :smirk:


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OfflineMikeOLogical
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Re: No jars will germinate. Again [Re: turtlelamp]
    #2300217 - 02/04/04 12:38 PM (12 years, 9 months ago)

gotta use bottled spring water... hard tap water will stall you out every time... according to PF, spring is better than purified or distilled, something about the mineral content.


--------------------
We got Nothing!
we're no longer selling jars.  :laugh:


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Invisibleturtlelamp
n00b
Registered: 11/27/03
Posts: 93
Loc: The Midwest
Re: No jars will germinate. Again [Re: ]
    #2300332 - 02/04/04 01:09 PM (12 years, 9 months ago)

No, the lids aren't inverted, but I wouldn't imagine that would halt germination... just slow colonization a little.

God dammit- I'm going to have to go buy bottled water now, aren't I? Arg. I wish I had well water...


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OfflineKaptKid
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Re: No jars will not germinate. Again [Re: turtlelamp]
    #2300449 - 02/04/04 01:51 PM (12 years, 9 months ago)

turtlelamp I've been having the same probelm as you.
On my 3nd set.
1st and 2 nd used tap water NO growth what so ever.
3rd time used bottle spring water. After 5 days only one site that
may be mycelium. Don't know for sure, have not seen any yet.
Did this on BRF as per PF tek
Have used 2 different spore supplies.
Been happly with water content of the cake and using tub in tub for heat.
I'm going to try WBS to see if I can do any better with it.


--------------------
Child of the 60's, Tripping ever since.


:sun:


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Anonymous

Re: No jars will germinate. Again [Re: turtlelamp]
    #2300784 - 02/04/04 03:06 PM (12 years, 9 months ago)

Quote:

No, the lids aren't inverted




i knew it. :lol:    yes, it matters.

if your doing the PF tek, you gotta have the lids inverted on your jars :lol: no wonder you aint got any growth man, the co2 must escape SOMEHOW.  thats also why you turn the jars over on their lids when growth halts...its due to co2 build up.

newbies  :rolleyes:


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Invisibleturtlelamp
n00b
Registered: 11/27/03
Posts: 93
Loc: The Midwest
Re: No jars will germinate. Again [Re: ]
    #2300833 - 02/04/04 03:15 PM (12 years, 9 months ago)

There are four holes in each jar.  Additionally, the CO2 doesn't build up until the organism actually produces it.  The mycelium can't produce it if the mycelium doesn't exist.  I'm not getting anything.  At all. :confused:


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Invisiblesimplemachine
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Registered: 09/14/03
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Re: No jars will germinate. Again [Re: ]
    #2300846 - 02/04/04 03:18 PM (12 years, 9 months ago)

It's been a long time since I used the original pf tek, but when I did it never seemed to make a difference in germination. gas xchange is important, but it's FAR from essential at this point in the program. If you want gas xchange use foil or polyfill or tyvec. Those are WAY better than loose lids.

I think your diagnosis is off. If it was an air xchange problem the spores would at least germinate, then stall, once they had used the O2 that is already sealed in the jars. Without mycellium growing, there is no respiration taking place, no 02 used, no CO2 buildup. Something else is causing these spores not to germinate.

Try the bottled water. Water is the only common factor between all jars. Testing that would shed some light, or at least eliminate a possible cause.


SM


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Edited by simplemachine (02/04/04 03:19 PM)


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Invisibleturtlelamp
n00b
Registered: 11/27/03
Posts: 93
Loc: The Midwest
Re: No jars will germinate. Again [Re: simplemachine]
    #2300917 - 02/04/04 03:30 PM (12 years, 9 months ago)

I agree. I will buy some bottled water tonight. I was concerned for a while that I'd ruined my jars by soaking them in bleach for a week, but I've since bought new jars. Once I've tried a different source of water, every factor I can think of will have been changed.


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Anonymous

Re: No jars will germinate. Again [Re: simplemachine]
    #2300928 - 02/04/04 03:32 PM (12 years, 9 months ago)

oh yeah :crazy: .  stupid ass newbies thinkin they know it all  :tongue:  my bad, no excuse for being a fucking idiot here, huh  :lol:  I was just stuck on the lid shit, cuz i sensed you didnt have the fuckers inverted, then forgot all about the issue at hand, im the newbie :nut: im crazy like that


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Invisiblesimplemachine
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Re: No jars will germinate. Again [Re: turtlelamp]
    #2300932 - 02/04/04 03:32 PM (12 years, 9 months ago)

I use bleached and brand new unrinsed jars all the time. no problems there.


SM


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