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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 8 months, 26 days
Re: Post Your Cultivation Picture of The Day [Re: the_r3dz]
    #22977374 - 03/05/16 08:05 PM (8 years, 2 months ago)

Quote:

the_r3dz said:
Did you inoculate it with a spore syringe? If so, then there's tons of reasons it could be contam'd.

this was buried

How would that happen? Stirring around too much air in your SAB moving your arms in and out, forgetting to spray it, things like that? I guess I don't understand how things get contam'd in an SAB if the air isn't moving.
They say not to hold your hands or anything above an open jar or plate, does the contam fall off and then gravity carries it?



Tissue sample to agar, agar to agar, I think I'm on transfer 4 or 5.
The PE was MS to agar, then agar to agar about 4 times also.

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Invisiblethe_r3dz
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Registered: 03/26/15
Posts: 5,709
Re: Post Your Cultivation Picture of The Day [Re: YaMoonSun]
    #22977380 - 03/05/16 08:07 PM (8 years, 2 months ago)

Oh it's you, sorry dude didn't read the name. Without an avatar I just assume it's some noob. Even Mike at first >.<

anywho. You should test it on agar first just to be sure.

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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 8 months, 26 days
Re: Post Your Cultivation Picture of The Day [Re: the_r3dz]
    #22977407 - 03/05/16 08:14 PM (8 years, 2 months ago)

No problem. I took it away from myself after countless failures and gave myself a new title.

I did LC to agar twice. First time it looked like black mold was being attacked by my isolate. Now it looks like trich surrounded by a random mold. Not cobweb. Not cubenis.


Edited by YaMoonSun (03/05/16 08:14 PM)

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InvisibleInocuole
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Re: Post Your Cultivation Picture of The Day [Re: YaMoonSun]
    #22977423 - 03/05/16 08:19 PM (8 years, 2 months ago)

Either you're skipping some important procedural steps somewhere or you just really need to upgrade a few things like your SAB... agar is generally pretty easy with the right tools.


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InvisibleAdden
I'm a teapot
Registered: 06/04/03
Posts: 39,201
Loc: Flag
Re: Post Your Cultivation Picture of The Day [Re: Inocuole] * 1
    #22977461 - 03/05/16 08:30 PM (8 years, 2 months ago)

If I can do agar work with shaky hands and nerve damage, I don't see why someone wouldn't be able to do it. It really is awfully easy.

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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 8 months, 26 days
Re: Post Your Cultivation Picture of The Day [Re: Inocuole]
    #22977472 - 03/05/16 08:34 PM (8 years, 2 months ago)

My agar hadn't been a problem, it's the LC that sucks. Or possibly the syringe.
Am attempting to sterilize it in the PC for once, so that may help. Honestly have half a mind to toss all my GT trays and the LC and just start a new agar tray from spores. That, and make a new LC from PE.

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InvisibleInocuole
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Re: Post Your Cultivation Picture of The Day [Re: YaMoonSun]
    #22977485 - 03/05/16 08:38 PM (8 years, 2 months ago)

That's... kinda part of why I don't use LC.


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 8 months, 26 days
Re: Post Your Cultivation Picture of The Day [Re: Inocuole]
    #22977531 - 03/05/16 08:50 PM (8 years, 2 months ago)

Well, I haven't done a LI yet, I'll have to put that on my list of things to do.
My fist LC colonized quite well. Long ago. I had to throw away the spawn and all my supplies though because of a snitch. Was a shame, because it was supposed to be a mutant that grows three shrooms from the cap of another shroom.

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OfflineBlu33
not anybody anyway....
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Registered: 10/31/15
Posts: 321
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Re: Post Your Cultivation Picture of The Day [Re: the_r3dz]
    #22977539 - 03/05/16 08:53 PM (8 years, 2 months ago)

Quote:

the_r3dz said:
Oh it's you, sorry dude didn't read the name. Without an avatar I just assume it's some noob. Even Mike at first >.<

anywho. You should test it on agar first just to be sure.




I was always under the impression that the only way to know if youve carried a contam is to fruit the myc, I know Myc fruits on agar sometimes, Maybe Like a mini PF jar or something would be a better way to find contams on that.:thumbup:


^^I thought this was uncommon, And Myc willing to fruit on agar is sign of vigor in a culture?^^


--------------------
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Edited by Blu33 (03/05/16 08:56 PM)

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OfflinePinPornProducer
Buy the ticket, take the ride
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Registered: 08/23/14
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Re: Post Your Cultivation Picture of The Day [Re: Blu33]
    #22977575 - 03/05/16 09:06 PM (8 years, 2 months ago)

Throwing darts, I just went out on 121. 2 bulls and a trip 7 :rockon:

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InvisiblebodhisattaMDiscordReddit
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Re: Post Your Cultivation Picture of The Day [Re: PinPornProducer]
    #22977584 - 03/05/16 09:09 PM (8 years, 2 months ago)

it's rare I get a plate that doesn't pin after it gets over grown

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OfflineAusMush
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Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s... Flag
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Re: Post Your Cultivation Picture of The Day [Re: bodhisatta]
    #22977589 - 03/05/16 09:11 PM (8 years, 2 months ago)

Ps subaeruginosa wouldn't pin on agar tho would it lmao , maybe if you left it for a year :rofl2:

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InvisibleAdden
I'm a teapot
Registered: 06/04/03
Posts: 39,201
Loc: Flag
Re: Post Your Cultivation Picture of The Day [Re: AusMush]
    #22977605 - 03/05/16 09:14 PM (8 years, 2 months ago)

I've still got some of my first set of azure transfers hanging around. Leaving them until they fruit or die. My mycelium still isn't going rhizomorphic yet, and I'm at a point in which I'm going to try a run at alder water agar. Every time I get a small piece of potential rhizo, it goes tomentose.

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OfflineSloppyJoseph
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Registered: 12/08/15
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Re: Post Your Cultivation Picture of The Day [Re: PinPornProducer]
    #22977611 - 03/05/16 09:17 PM (8 years, 2 months ago)

Quote:

PinPornProducer said:
Throwing darts, I just went out on 121. 2 bulls and a trip 7 :rockon:




Ok?


Some pins starting to come through 9 days after dunk. Feeling like it took a little long. The big one was the first pin. Inkling that it aborted. The black part of the cap is an indication of such, right?


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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: SloppyJoseph]
    #22977615 - 03/05/16 09:18 PM (8 years, 2 months ago)



My second truffle harvest, 1.75 lbs give or take a couple pounds


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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: bodhisatta]
    #22977618 - 03/05/16 09:20 PM (8 years, 2 months ago)

Quote:

bodhisatta said:
it's rare I get a plate that doesn't pin after it gets over grown





My plates never pin what gives? Do I use too much film?


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OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 1 year, 3 months
Re: Post Your Cultivation Picture of The Day [Re: SloppyJoseph]
    #22977621 - 03/05/16 09:20 PM (8 years, 2 months ago)



25 PastyPlates ready for sterile work. Growing out some cubes I'm trying to isolate and a couple Aloha edible cultures


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OfflineSloppyJoseph
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #22977622 - 03/05/16 09:20 PM (8 years, 2 months ago)

So how do those truffles taste? Anything like shitty fresh cubes or better?


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OfflinePinPornProducer
Buy the ticket, take the ride
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Re: Post Your Cultivation Picture of The Day [Re: SloppyJoseph]
    #22977631 - 03/05/16 09:25 PM (8 years, 2 months ago)

Nutty like a walnut kinda

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OfflineMysticMoteToter
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Registered: 07/26/15
Posts: 2,036
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Re: Post Your Cultivation Picture of The Day [Re: SloppyJoseph]
    #22977632 - 03/05/16 09:26 PM (8 years, 2 months ago)

I don't think cubes taste that bad..especially wet


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