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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: micro]
    #22470159 - 11/03/15 09:22 AM (8 years, 6 months ago)

Quote:

micro said:
huh, weird... generally it is to absorb some unwanted compound





Or to provide a really cool looking black background.

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Offlinedrake89
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #22472863 - 11/03/15 07:23 PM (8 years, 6 months ago)

Quote:

Alan Rockefeller said:
Quote:

micro said:
huh, weird... generally it is to absorb some unwanted compound





Or to provide a really cool looking black background.




everyone I've seen that has tried it says that it makes mycelium grow faster.  i reckon it's a readily available form of carbon but that's just a guess.


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Fiery Fungi (like us on faeboo)

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OfflineMrcloudy
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Re: Pestalotiopsis microspora [Re: drake89]
    #22472933 - 11/03/15 07:37 PM (8 years, 6 months ago)

I am using it to try to increase spore germination in Ganoderma. One of the studies I read used activated carbon in conjunction with Rhodotorula yeast to germinate mycorrhizal spores. The study suggested that autoclaving the agar produces inhibitory substances and the addition of activated carbon helps to clear these up.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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OfflineKingSloth
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Re: Pestalotiopsis microspora [Re: Mrcloudy]
    #22473066 - 11/03/15 08:04 PM (8 years, 6 months ago)

Quote:

Mrcloudy said:

The good news is though that if your culture produces those little black liquid balls... you now have tons of spores to make more cultures with and see if you can't find one that does a better job at eating the plastic than the parent culture.





Aren't the black dots pools of conidiospores? Since conidiospores are asexual they'd be genetically identical to the parent, right? Or is there genetic variation in conidiospores?

In case there is, i've tried germinating some in 10% and 5% PUR. They are germinating fine in the 5% but having a much harder time in the 10%. I figure the ones that do survive might have better PUR resistance/degredation abilities.

I've also made some plates of agar mixed with PUR though somewhere in the process there was contamination of Black Mold. This turned out to be kind of lucky since black mold was used as a positive control in the article since it also has plastic eating capabilities. http://www.jairjp.com/NOVEMBER%202012/08%20RAAMAN.pdf

There were no zones of clearance on the contaminated plates so I assume that this method doesn't work. I think for a zone of clearance to happen the media needs to be liquid. I've started some plates with liquid MEA and PUR. I've done one plate with a agar wedge and another with the condiospores. After 3 days a zone of of clearance seems to be forming around the wedge and all across the condiospore inoculated plate!



Edited by KingSloth (11/03/15 08:09 PM)

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OfflineMrcloudy
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Re: Pestalotiopsis microspora [Re: KingSloth]
    #22473205 - 11/03/15 08:27 PM (8 years, 6 months ago)

Quote:

KingSloth said:


Aren't the black dots pools of conidiospores? Since conidiospores are asexual they'd be genetically identical to the parent, right? Or is there genetic variation in conidiospores?






Not sure, you are probably right, they seem to be a form of conidia which are asexual. It seems these spores observed will make essentially a clone, if I understand conidia correctly.


The genetics of Pestalotiopsis is odd.

Quote:



Sexual and asexual forms

One fifth of all known anamorphic fungi lack known sexual
states (Shearer et al. 2007), and out of 2,873 anamorphic
genera names, 699 genera and 94 anamorph-like genera are
linked to a sexual state (Hyde et al. 2011). The links
between sexual and asexual stage are mostly from indirect
evidence, with some links known through experimental
or molecular data (Kendrick 1979; Reynolds 1993;
Shenoy et al. 2007; Hyde et al. 2011). Pestalotiopsis is
a species-rich anamorphic genus with species mostly
lacking sexual morphogenesis, unlike the coelomycetous
genera Colletotrichum and Phyllosticta (Armstrong-Cho
and Banniza 2006; Wulandari et al. 2009) and Penicillium
(Cannon and Kirk 2000). The sexual states or teleo
morphs of Pestalotiopsis species have been identified as
Pestalosphaeria (Barr 1975) and Neobroomella (Kirk et
al. 2008).
The asexual Pestalotiopsis state and ascomycetous
sexual state have rarely been recorded in the same host
plant (Barr 1975; Nag Raj 1985; Hyde 1996). However, it is
not always clear that the two stages found are definitely the
same biological species and therefore molecular evidence is
needed to link them. In the laboratory species of Pestalotiopsis
rarely develop sexual forms (Metz et al. 2000). Zhu
et al. (1991) induced Pestalosphaeria accidenta P.L. Zhu,
Q.X. Ge & T. Xu and P. jinggangensis P.L. Zhu, Q.X. Ge &
T. Xu to form on potato dextrose agar (PDA). However, this
took 5 to 6 months of incubation. Metz et al. (2000)
obtained the sexual state of P. microspora, an endophytic
isolate that produced taxol. The asexual stage formed after
3–6 weeks on water agar with dried yew needles when
incubated at 16–20 C with 12 h of light per day and was
identified as Pestalosphaeria hansenii Shoemaker & J.A.
Simpson. The twelve sexual states known for species of
Pestalotiopsis are listed in Table 1.




--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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Offlinestealthsquirrel
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #22511668 - 11/11/15 09:35 PM (8 years, 6 months ago)

Could I get some as well? U.S.
ill trade or pay either one
I have blue oyster, amanita muscaria, Panellus stipticus (luminescent fungi), and black truffle

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OfflineJim Morrison
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Re: Pestalotiopsis microspora [Re: nomendubium]
    #22674958 - 12/19/15 07:28 AM (8 years, 4 months ago)

Quote:

nomendubium said:

    I am passing these out to people who paid and got screwed over. I am making 45 dishes right now. I can't cover the shipping. I know it's a bitch, but I NEED about $4 and You will get an entire dish. Get with Ghiajake about it because he has the list of who paid. I'm not just sending these to everyone who wants one, only people who paid. I am doing it out of the kindness of my heart, black as it may be




Someone on Ebay is trying to sell these cultures for $80, and using this exact picture to do it. Is that you on Ebay?


--------------------
Jim Morrison's Trade List

Live Long and Myceliate!

The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand

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Offlinenomendubium
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Re: Pestalotiopsis microspora [Re: Jim Morrison]
    #22674966 - 12/19/15 07:36 AM (8 years, 4 months ago)

no, it is not. I am selling them for $20 and I am not profiting off of it. Also that price was not set by me


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Re: Pestalotiopsis microspora [Re: nomendubium]
    #22674974 - 12/19/15 07:43 AM (8 years, 4 months ago)



--------------------

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OfflineJim Morrison
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Re: Pestalotiopsis microspora [Re: nomendubium]
    #22675011 - 12/19/15 08:05 AM (8 years, 4 months ago)

Quote:

nomendubium said:
no, it is not. I am selling them for $20 and I am not profiting off of it. Also that price was not set by me



Yes I think I read somewhere in this huge thread, about the co-op. That's a good deal, but $80?


--------------------
Jim Morrison's Trade List

Live Long and Myceliate!

The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand

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OfflineJim Morrison
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Re: Pestalotiopsis microspora [Re: nomendubium]
    #22675016 - 12/19/15 08:06 AM (8 years, 4 months ago)



--------------------
Jim Morrison's Trade List

Live Long and Myceliate!

The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand

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OfflineLittleDaddy
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Re: Pestalotiopsis microspora [Re: Jim Morrison]
    #22971470 - 03/03/16 11:50 PM (8 years, 2 months ago)

Hello,

I am interested in P. microspora if someone is asking for a reasonable price.

Thank you.

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OfflineJim Morrison
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Re: Pestalotiopsis microspora [Re: LittleDaddy]
    #22971637 - 03/04/16 02:06 AM (8 years, 2 months ago)

Quote:

LittleDaddy said:
Hello,

I am interested in P. microspora if someone is asking for a reasonable price.

Thank you.



I would love to help you out. I had a trade with SineWaveHighway, but he's totally disappeared, with my petri dishes, and I got nothing. Don't think he's been on since middle of November 2015. Stay away from that one if he offers.


--------------------
Jim Morrison's Trade List

Live Long and Myceliate!

The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand

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Invisiblewoodrow
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Registered: 03/17/03
Posts: 142
Re: Pestalotiopsis microspora [Re: Ganzig]
    #22971708 - 03/04/16 03:35 AM (8 years, 2 months ago)

Quote:

Ganzig said:
That is a good idea Alan. I was thinking about mixing plastics straight into
an agar solution as well as feeding plastics to it.




I question the use of agar as a medium for plastic eating microbes. There are such things as agar degrading microbes otherwise the oceans would be full of agar from seaweed by now. Instead, the oceans are filling with plastics which tells us that plastics are far more difficult to degrade. A mixture of agar and plastics would likely select for agar degrading organisms and leave the plastics untouched.

An ideal selective medium would consist of a solution of mineral salts with with a nitrogen source and plastics added as a sole source of carbon. Bits of degraded plastic could be added to this medium in the hope of enriching for something that can grow on plastic. The medium would likely need to be incubated in the dark to prevent the growth of algae.

I suspect anything that can grow on plastic would be a complex group of organisms rather than a single fungus.

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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: woodrow]
    #22971776 - 03/04/16 04:42 AM (8 years, 2 months ago)

Agar can grow many fungi, including plastic eating ones.  Good idea about making agar with plastic as the sole nutrient source.  Then you could throw all sorts of crap on it and see what grows.

My Pestalotiopsis microspora cultures are doing well, lots of conidiaspores, but it doesn't eat the petri dish.  But I guess it is not supposed to, as the dishes are not polyurethane.

Has anyone had any success getting the P. microspora that we have to eat any kind of plastic?  Or any failures?

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Invisiblewoodrow
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #22973558 - 03/04/16 04:55 PM (8 years, 2 months ago)

Quote:

Alan Rockefeller said:
Good idea about making agar with plastic as the sole nutrient source. 


Agar is a carbon source for many organisms and microbes will consume the most readily available carbon source first and then move on to the more difficult. If you mix plastic and agar, the agar will be the first consumed and you will be selecting for organisms that break down agar but not necessarily plastic so agar plus plastic is a poor screening tek.

Nutrient agar is good for maintaining stock cultures but it is not good if you are screening for plastic digesters. Some of the teks described earlier used agar plus polyurethane and they looked for zones of clearing as evidence of plastic digestion and this is a good secondary test once you have found an organism to work with but it would not be good as a selective medium because it has two carbon sources- agar and plastic and agar is certain to be the most digestible.

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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: woodrow]
    #22973839 - 03/04/16 06:11 PM (8 years, 2 months ago)

So a good selective medium would be just plastic?

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OfflineMrcloudy
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #22974111 - 03/04/16 07:31 PM (8 years, 2 months ago)

Liquid with a water based polyurethane mixed in perhaps? Sterilizing is difficult though as the polyurethane likes to separate when heated.

I haven't had any success yet, though I haven't tried more than a few plates so far.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: Mrcloudy]
    #22974185 - 03/04/16 07:52 PM (8 years, 2 months ago)

Perhaps it is possible to sterilize at room temperature using some kind of radiation?    UV?  Xrays?  Gamma rays?

I imagine a new container of polyurethane would be completely sterile anyway. 

Why sterilize at all?  If anything at all grows, that's a win.

Maybe it is better to throw as much dirty crap in there as you can - the dust from an air filter for instance, to try to give thousands of different organisms a chance to eat the plastic.


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OfflineMrcloudy
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #22974310 - 03/04/16 08:38 PM (8 years, 2 months ago)

good point. :thumbup: 

Easier to work with non sterile materials anyway, maybe I will mix up some liquid culture mix when I get home and throw some Pestalotiopsis in.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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