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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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Should I use Extra Light MEA? Just realized I got the regular mea. Will it be really hard to see through?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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You don't need to be able to see through it. Your adding food coloring if you follow the tek. What matters is whats on the surface.
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,178
Loc: rural ghetto
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Pre-sterilized individually foil wrapped cotton buds to swipe the spore print to agar: is this a good way to streak some spores, anyone?
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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Is it okay if water is touching the bottom of the containers? Will this cause condensation issues? Should I elevate the bottom row above the water? I know you didn't mention it but i'm just mainly wondering how much you use.
Also I'm wondering if the water in the PC should be room temp when I start and slowly bring it up to temp?
Edited by tombosley8 (02/23/16 02:27 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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I like to elevate it simply to prevent the water from jostling the containers about. This helps ensure they are still level when they cool.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 4 days, 9 hours
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what ratio should I use for that wheat flower? what ammount of potato flakes should I use for good rhizomorphic growth?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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2%
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kushroom



Registered: 12/04/14
Posts: 588
Loc: I'm lost
Last seen: 5 months, 5 days
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Ive made some plates before and they worked well. I never covered tho but thab again i was using baby jars and my own sort of tek with the recipe from this tek. IIm outta baby jars nd dont come across them much so i bought the pp5 containers. Is it kool to just tape it nd pc instead of stuff with paper towel than aluminum foil?
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 All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 4 days, 9 hours
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Re: Pasty Agar Tek [Re: kushroom]
#22942276 - 02/24/16 07:37 PM (8 years, 10 hours ago) |
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it's gotta be micropore tape. get it at walmart or rite aid in the pharmacy section. no-one is just stuffing their holes with paper towels
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 1 year, 23 days
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Quote:
tryptkaloids said: it's gotta be micropore tape. get it at walmart or rite aid in the pharmacy section. no-one is just stuffing their holes with paper towels
^ yep. I had problems finding micropore at first so I used transpore- sometimes it wouldn't survive pcing so I had to reapply, and I had about a 8% contamination rate by using it vs 0 with micropore. (Judging solely on pre-inoculation and agar transfers- not MS or clones)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Waterproof tape, polyfil, and SFD's are also great substitutes.
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oregon.trailboy
OTB



Registered: 12/15/14
Posts: 1,641
Loc: Oregon
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Pasty did you try a no hole PC run with the lids just slightly cracked and covered in foil
Once done Pc'ing an cool just simply close lids as you remove from PC?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Yeah I did. It worked for the first run but was actually a real pain to get the lids to sit just right. After a first run the lids expand just enough so they don't want to sit cracked in the PC and all got sucked down and crushed. I decided it wasn't worth the effort compared to drilling a hole and slapping some tape on.
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kushroom



Registered: 12/04/14
Posts: 588
Loc: I'm lost
Last seen: 5 months, 5 days
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Alright got the micropore, now i can see why to cover it with tin foil but is it that much of a difference between covering with paper towel or not?
-------------------- :/
 All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
Edited by kushroom (02/26/16 01:29 PM)
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Pasty Agar Tek [Re: kushroom]
#22948608 - 02/26/16 01:35 PM (7 years, 11 months ago) |
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Quote:
kushroom said: Alright got the micropore, now i can see why to cover it with tin foil but is it that much of a difference between covering with paper towel or not?
micropore tape is waterproof, a paper towel is not.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 4 days, 9 hours
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Quote:
Munchauzen said:
Quote:
kushroom said: Alright got the micropore, now i can see why to cover it with tin foil but is it that much of a difference between covering with paper towel or not?
micropore tape is waterproof, a paper towel is not.
Really? I didnt know this. Why do we even use paper towels then?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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I use it because sometimes the heat melts the glue of the micropore tape to the foil. This can tear the tape when unwrapping. I also prefer not having my filter material soaked if at all possible. Finally I like to write notes on it rather than mucking around with masking tape.
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kushroom



Registered: 12/04/14
Posts: 588
Loc: I'm lost
Last seen: 5 months, 5 days
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Thank you very much for the detailed reply! I definitely will put it on there. Awesome right up to man ive been using this tek since i started agar and its never failed me yet. Thanks man!
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 All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 4 days, 9 hours
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Re: Pasty Agar Tek [Re: kushroom]
#22948895 - 02/26/16 02:48 PM (7 years, 11 months ago) |
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Is it safe to use sfds on my LI jar lids?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Quote:
Pastywhyte said: I use it because sometimes the heat melts the glue of the micropore tape to the foil. This can tear the tape when unwrapping. I also prefer not having my filter material soaked if at all possible. Finally I like to write notes on it rather than mucking around with masking tape.
I've only had one out of god knows how many get the paper towel wet. If you fold it right it'll lock right in kind of. I like them because they help plates dry faster in SAB and by the time they're done I just get to work. I'd never skip the paper towel as this seems to work just dandy.
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