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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK 7
#22929639 - 02/21/16 11:59 AM (7 years, 11 months ago) |
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Hey Shroomerites,
Tonight I'm bringing you guys a little insight into how I do Liquid Culture! I don't know if I would call it a Tek as liquid cultures are pretty wide practiced and varying In how they are done.....but I know I only got to this point by piecing together very widely scattered information! It is my aim to compile here a one stop source for how to do a liquid culture from preparation all the way to the point of using as an inoculant for grains or brf or a bottle tek recipe, or even a new LC!
Introduction
Let's start by talking a little about the appeal of the Liquid Culture...
Even when we were just starting off with our first ever Spore syringes the idea of turning 10ml into 3000mL of inoculant was so alluring...we've since done our homework, gotten our work in, and now we know the only great way of starting a Liquid Culture is to have a culture that we know is clean and sexy AF. Today, I am still in love with the idea of exponentially increasing my inoculant stock all the time, and really....why expand one isolate plate to a dozen more to inoculate a couple dozen grain jars via wedge when I can inoculate 10 liquid culture jars from one isolate plate to inoculate 3000 grain jars....
but what about Grain to Grain Transfers? Are LC's easier than that? In my humble opinion....yes! Even now a days where one piece plastic jar lids are becoming more and more popular than the classic metal disc/ring lids, flame sterilizing a needle and injecting it through an inoculation port is easier than opening two jars and spilling some of one into the other...even saying it like that should paint a picture of the world of difference...when inoculating grains with liquid inoculant you don't have to take lids off any jars...which even in a well tested and proven STill Air or scrubbed air environment...taking lids off and putting them back on is intimidating....on top of that needle work inoculation just goes super fast.....
The bottom line is that liquid culture very very effectively reduces labor by eliminating the need to expand clean plates to multiple plates for grain inoculation...also... A rice sized wedge can generate hundreds and hundreds of mL's of inoculant...and in a community where isolating Devine genetics makes you a god....there's hardly a better way to expand a culture....but what is the draw back?
The drawback is that in order to confidently work with a Liquid culture you must have a good foot into hardcore sterile technique, and very likely in this case....cleaning up cultures via agar work....while the following procedure might be successful using a syringe of multi spore solution, it is most securely carried out using clean culture from agar transfer. (Also note that using agar wedges eliminates the chance that spores will not collide and germinate)
That being said, I will work to make sure those ballsy enough to do this with a spore syringe understand where the procedure differs from Agar wedge inoculation...but anywho....let's start with talking about the liquid culture vessel...I like regular mouth pint size mason jars, but that's the easy part....the hard part comes with tackling a decent LC lid:
BUILDING A CUSTOM LIQUID CULTURE JAR LID So when we are working with a Liquid Culture we have a couple things to consider for our housing vessel. As always with colonizing mycelium...gas exchange is a must, and in the case of a liquid culture we need a way to extract our inoculant without compromising cleanliness....in my time cultivating mushrooms, I have managed to design and build a lid that suits these needs on an impressively consistent level. The following liquid culture jar lid build is not exactly my original idea, but certainly in this case my own design and we will need:
Materials for Lid -Jar Lids w/ bands (all examples will feature metal lids, but plastic lids are expected to do as well) -High Temperature Silicon -1/4" Plumbing Inserts -Polyfill -Pliers -Drill w/ 1/4" Bit -Tyvek (in this case painter coveralls cut to size) -Micropore tape
     
Drill and pliers have been left off the picture list. Lid Procedure: -Drill two 1/4" holes in your jar lid across from each other

what is a SHIP SHIP stands for self healing injection port.... It means you can stick a needle through it and it seals itself afterward...good for keeping LC's contam free....
I'll clean up this section in the next week...but what we are doing is we build a Self Healing Injection Port by extruding the silicon into the hole and making it neat by sandwiching it with paper while it cures...
In the other hole we put our plumbing insert and gasket it with the silicon to the lid....
It comes out to this:
  
Once the SHIP is made and the insert is firmly in place, put a small wad of poly fill in the insert and using your pliers crimp the insert....see pictures above. Over the top side of the insert put a small piece of painters coverall secured with micro pore tape....again see picture above. The brass inserts can be purchased from hardware stores (Lowes, Home Depot, Ace). This serves as our gas exchange! The plumbers insert protects our polyfill from getting wet maintaining a clean dry filter for gas exchange!
Once the lid is constructed we are ready to talk about the chemical makeup of the Liquid Culture
Liquid Culture Preperation So this recipe comes at great trial and ERROR....

We use a pint size jar for this...and HONEY....the label needs to say 100% Natural Honey
We use 285mL's water and 15g 100% organic natural honey. Generally we will get our honey into the jar by dissolving it in water and adding it to the jar....should be very clear prior to sterilization:


We steam sterilize in the pressure cooker at 15PSI for 12 minutes. After trial after trial....12 minutes seems ideal for water/honey....to minimize sediment but maximize sterilization!
After sterilizing for 12minutes and letting cool for several hours, we are ready to inoculate our liquid culture....for this we'll need:
A clean agar plate culture:
 
In a Still Air Box or in front of a Laminar Flow Hood, follow proper sterile technique to cut a wedge from the selected plate and drop it into the Liquid Culture solution..replace the lid then store at incubation temps for colonization for about a week (the ballsy among us might try inoculating with spore syringe through the SHIP after flame sterilizing the needle):
it should be noted that it is NOT advisable to use spores to inoculate a Liquid Culture However, in my logged experience (found HERE. )This was three years ago when I was just starting out, and again I'll say now I know better...but I did it. By now I have found agar so manageable to secure clean cultures that not using an agar wedge is laughable if not for the only reason that it just makes spore syringes last forever as plates take just a drop of spore solution to germinate....BUT...if you insist on using spore solution, go ahead and inject 1cc of spore solution into your LC through your SHIP after flame sterilizing the needle. For those of you not wanting to sipherr through a three year old grow log, here's what happened 3 years ago just starting out:
Quote:
Mycologist217 said:
Quote:
cronicr said:
 i'm just buggin bud bet seriously

Please keep in mind that these were inoculated last Monday...Honey/Water LC (inoculated with spore solution)

But a most secure way is via agar wedge drop...
   
After about a week you should see very distint mycelial growth,,.maybe even rhizomorphs apparent in solution....at this point you should prepare and sterilize spawn or substrate to apply your LC to via syringe....
How to Sterilize an Empty Syringe I have regularly used recycled syringes that originally came filled with spore solution from my preferred vendor! THE particular vendor uses very sturdy syringes/needles! When you load your pressure cooker with your preferred substrate, also include your empty syringe wrapped in foil!
-remember to sanitize the top side of your SHIP. I like to use cotton balls saturated with 70% isopropyl alcohol for this-
Once the grain jars are cooled and loaded into the SAB, I give my LC a very good shaking! Then I go ahead and unwrap my syringe, uncap it, and flame sterilize the needle, WITHOUT LETTING THE NEEDLE COOL we plunge through our SHIP and draw up a syringe worth (usually 10cc's) of LC....now we are ready to proceed with inoculating our grains, or cakes. Or bottle substrates or a new LC or what have you.....
Make sure to flame sterilize between each vessel you inoculate....

I have emptied and filled the syringe with LC multiple times during several inoculations....any time you are holding an empty syringe....hold the needle tip over your sterilization flame!!
Following these steps you should have a very clean inoculation on your hands! Welcome to the world of Liquid Culture!
Testimonial Grain Jars and Monotubs grown using this LC tech:
            
Thanks again for reading!
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
Edited by Mycologist217 (12/06/16 08:42 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217] 1
#22929688 - 02/21/16 12:17 PM (7 years, 11 months ago) |
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Nice writeup. I personally like Whatman filters but the crimped brass looks like a good idea too. I also like the no guff explanation regarding spores to liquid media. All in all good job
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
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Last seen: 8 months, 15 days
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Pastywhyte]
#22930978 - 02/21/16 05:45 PM (7 years, 11 months ago) |
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Thanks Pasty! I'll work on getting this write up cleaned up and a little more filled out this week....make an LC lid with a plastic storage lid....but thanks for the support.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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cronicr



Registered: 08/07/11
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#22930992 - 02/21/16 05:49 PM (7 years, 11 months ago) |
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sweet write up, great job man.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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SloppyJoseph
Non-sporalator



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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: cronicr]
#22931334 - 02/21/16 07:32 PM (7 years, 11 months ago) |
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Sweet dude! I really like this I was going to go to Lowes tomorrow I think i'll look for these brass inserts and get some silicone. I've been wanting to work with LC I haven't given it much thought but I have been getting impatient with how long agar takes to jump to grain and how many plates you have to make to knock up any decent amount of jars. Thanks for this man it's inspired me
-------------------- AMU Q&A
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Rocket

Registered: 03/06/10
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#22933112 - 02/22/16 10:38 AM (7 years, 11 months ago) |
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Good work, Mycologist217.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Rocket]
#22938577 - 02/23/16 07:28 PM (7 years, 10 months ago) |
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Thanks, Rocket...I've put pretty much all of the last two months into getting this together....about a dozen different LC's were made at different sterilization times....definitely the hardest part is reducing sediment but maintaining sterility....which is why I go for a fairly high 5% Honey to 95% water....the sediment is literally cooked/burnet sugars...and we don't have a lot of it to waste....
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Rocket

Registered: 03/06/10
Posts: 3,653
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#22940861 - 02/24/16 01:12 PM (7 years, 10 months ago) |
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The recipe:
• 285ml water
• 15g 100% organic natural honey
• PC at 15PSI for 12 minutes to minimize sediment but maximize sterilization
For those who don't have a scale, (I currently don't) about how many ml is 15 grams of honey?
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Mycologist217
Frank's Disciple



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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Rocket] 1
#22941359 - 02/24/16 03:44 PM (7 years, 10 months ago) |
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It's about 5/6th of a table spoon...probably be fine with a whole table spoon.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
Edited by Mycologist217 (02/24/16 03:48 PM)
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Psilosoulful
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#22941380 - 02/24/16 03:49 PM (7 years, 10 months ago) |
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lovepotion
Unconditional


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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Psilosoulful]
#22941449 - 02/24/16 04:06 PM (7 years, 10 months ago) |
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Double thumbs up   Thank you for keeping things simple, cheap and effective.
One thing I want to understand is- is it really no problem pressure cooking syringes for 90 mins with the substrate? Ive got a few empty's
-------------------- Staying Out to Lunch
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: lovepotion]
#22941666 - 02/24/16 05:10 PM (7 years, 10 months ago) |
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Wrap in foil...yeah I sterilized it empty wrapped in foil at this point 5 times...I keep the needle clear by flaming it and blowing out anything inside a few times...sometimes a little polyfill melts onto the needle when you inject it in the grain jars...sometimes the interval flame sterilizing between jars is enough to totally clear the needle...I just work hard to keep them in good shape...no problem sterilizing several times at this point,
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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lovepotion
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#22941729 - 02/24/16 05:25 PM (7 years, 10 months ago) |
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Cant argue with those results. Really good idea clearing the needle before sterilizing. Cheers to keeping plastic out of landfills one spore syringe at a time. Thanks Myco!
-------------------- Staying Out to Lunch
Edited by lovepotion (02/24/16 05:27 PM)
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Dayton1994
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#22944678 - 02/25/16 12:17 PM (7 years, 10 months ago) |
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nice write up, man. just wanted to know why its not advisable to inoculate with spore syringe? contam introduction maybe? but isnt that what the self healing injecting port is for? Thanks!
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Dayton1994
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#22945766 - 02/25/16 05:08 PM (7 years, 10 months ago) |
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Do these lids serve the same purposes with brf pf tek jars? Had bad contam with last jars..
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Dayton1994
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Dayton1994]
#22945769 - 02/25/16 05:10 PM (7 years, 10 months ago) |
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And how about the lids for the agar? Will they benefit from the same lids? Does agar need the same fae?
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Mycologist217
Frank's Disciple



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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Dayton1994]
#22946156 - 02/25/16 07:08 PM (7 years, 10 months ago) |
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These lids are custom built for liquid cultures....again lids only require a very simple GE filter....a hole with tyvek and tape....brf cake jar lids just get tape over needle holes. The specific design of this gas exchange filter is meant to preserve the day integrity of the filter and thus keep it effective...without the brass insert shielding the poly fill/tyvek filter....the culture can wet it making a pathway for contamination....
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#23002399 - 03/13/16 11:30 AM (7 years, 10 months ago) |
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Check out my current grow log substantiating this LC manual! You can read it and view results HERE
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Mycologist217
Frank's Disciple



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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#23899285 - 12/06/16 08:44 AM (7 years, 1 month ago) |
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OP edited to include my signature (dont know why it wasnt there) which is chock full of Grow logs featuring this LC tech.
Also edited to include several pictures of monotubs and (WAY MORE IMPORTANTLY) grain spawn jars prepared using this Exact method. Thanks again for reading guys!
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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cronicr



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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#23900063 - 12/06/16 02:17 PM (7 years, 1 month ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,884
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: cronicr]
#24125162 - 02/28/17 02:29 AM (6 years, 10 months ago) |
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Maybe I'm misreading, but the wad of silicone is your SHIP, correct? So you're aspirating through an RTV SHIP in the LC jar and injecting the LC through a wad of Polyfil in jars that use Polyfil instead of SFDs?
If so, it doesn't seem like you'd aspirate much of the total LC with the size of needle you're using. Are you opening the lid to suck up LC at all? Or tilting the jar?
Also, not sure what this means:
Quote:
.but what we are doing is we build a Self Healing Injection Port by extruding the silicon into the hole and making it neat by sandwiching it with paper while it cures...
It looks like there's only silicone in place for the SHIP and no other paper or Tyvek you mentioned earlier. Seems like you only put surgical tape on top of the crimped plumbing insert to help keep the Poly dry?
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Mycologist217
Frank's Disciple



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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: stareatclouds]
#24125376 - 02/28/17 06:48 AM (6 years, 10 months ago) |
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i inject throught the silicon. There is polyfill inside the brass plumbing insert covered with tyvek and yes...brass keeps it dry.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Mycologist217
Frank's Disciple



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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#24226775 - 04/07/17 11:49 PM (6 years, 9 months ago) |
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I would love to get some testimonials if anyone has built and used this LC vessel lid.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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electricvelvet
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#24226804 - 04/08/17 12:16 AM (6 years, 9 months ago) |
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I too am most concerned with the lids of LC jars. This seems like it would keep the polyfill from being soaked all the way through; only the very bottom at most. Does anyone know if the whole filter medium has to be soaked through for contams to get in? Or just a little wet on one section? Regardless, the tyvek/micropore creates a second filter safe from moisture.
Are Whatman filters just immune to this sort of water-soiling?
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Cepheus16


Registered: 01/03/17
Posts: 292
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#24235603 - 04/11/17 06:41 PM (6 years, 9 months ago) |
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Aye! Cool lids man. Question though, you leave dont crimp the insert all the way do you.? If you did it should block GE. Anyways, here my go at them, i should be transfering an agar wedge to your lc formula in the next week.
how does it look?
Edited by Cepheus16 (05/17/17 09:22 PM)
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ScroopyNoopers
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: stareatclouds]
#25118373 - 04/06/18 12:55 PM (5 years, 9 months ago) |
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Hey, props to the write up. But I am curious why you go through so much work for that GE? Why couldn't you just cover it with a piece of tyvek only? I am working on my first LC, and I have a magnetic stir bar, and I don't plan on having to shake my jar so that it splashes the lid. It makes sense in my head.
Also, after inoculating the LC does it just go directly into the fridge? or does it need to stay warmer while the mycelium grows at first?
I appreciate your feedback very much, thanks for taking your time to read, and help me out.
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Mycologist217
Frank's Disciple



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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: ScroopyNoopers]
#25211448 - 05/17/18 09:46 PM (5 years, 8 months ago) |
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The reason the extreme measures on the filter is that wet means pathway for contamination. By encasing the filter in brass and making it elongated you can agitate the LC and keep the filter dry. A normal tyvek filter will contaminate.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Mr.Mush420
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#26002516 - 05/19/19 10:38 PM (4 years, 8 months ago) |
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Why use the filer at all I just put 2 ships in mine and to release the pressure I use an old syringe stuffed tight with Polyfil seems to give it enough oxygen I find lc doesn’t really need much fae to grow and use 5g light malt extract 20g dextrose to 500 ml water put like 250ml in 500ml jars and pc@15 psi 15 min take out and tighten lids as soon as pressure is released
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Professor X
School for the Gifted



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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mr.Mush420]
#26002606 - 05/20/19 12:06 AM (4 years, 8 months ago) |
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I've made lids using new 10ml chubby gorilla bottles stuffed with polyfil (I bought 100 for $12 lol) but this is an even cheaper and just as effective method that doesn't require drilling several 1/8 holes in semi rigid PET.
I make my own filters because I don't like ordering LAB equipment, I've been off the radar for 20 years I'd like to stay that way.
Edited by Professor X (05/20/19 12:07 AM)
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fricky00
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Mycologist217]
#26584802 - 04/07/20 07:27 PM (3 years, 9 months ago) |
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Is anyone inoculating mono-tubes with LC yet?
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Daddy4487
The creator

Registered: 08/18/20
Posts: 403
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Professor X]
#27242647 - 03/08/21 04:03 AM (2 years, 10 months ago) |
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Hey bud this was a nice simple informative write up.... I’ve heard of LC here and there but i didn’t really want to stray from something before I absolutely perfected it first( I’m just now starting to feel confident enough in my agar making/cleaning/contam identifying skills now). With that being said i mean it’s clear as day the huge advantage this plays over having to wait for 1 or 2 inoculation points to do their thing.. I absolutely need to benefit from the time which could be saved and everyone likes saving money,, lol I may seriously consider giving this a shot in the very near future. 8 (if I do I think I’ll just cough up the $ for SHIPS offline) but the one reason haven’t looked into LC sooner is because I heard that it’s not as successful say compared to just dropping wedges.... do You find this to be true?
-------------------- Hope I can help & Thanks for the help!
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Eve1
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Pastywhyte]
#28210889 - 03/02/23 11:09 AM (10 months, 21 days ago) |
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I've been doing a 4% solution 12 g Honey 300 mls of distilled water
I PC for 20 minutes
Inoculate with 4cc of a spore syringe
About Honey There's no such thing as organic honey Bees fly up to two miles in all directions, so there's no way they're not gathering pollen and nectar off untreated flora
There's only two states that have Honey standards. In the remaining states you can sell 8 ounces of honey and there might only be an ounce of actual honey the remaining volume is corn syrup
Real honey crystallizes ,the products in store retail don't crystallize
Where did you come up with 285 mls of water and 15 g Honey?
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stubb
Dahg Rastubfari


Registered: 03/23/19
Posts: 1,310
Loc: Memory
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: Eve1]
#28210926 - 03/02/23 11:35 AM (10 months, 21 days ago) |
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Quote:
Eve1 said: Bees fly up to two miles in all directions
That's why certified organic honey is produced at least six miles from any chemical producing human infrastructure, including agriculture.
Quote:
Real honey crystallizes ,the products in store retail don't crystallize
Filtered honey doesn't crystallize, that doesn't mean it's not 'real'.
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mycoloking
Mycologist Newbie


Registered: 01/01/24
Posts: 46
Last seen: 2 days, 13 hours
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Re: Mycologist217's Water/Honey LC (Liquid Culture) Manual - w/ Custom Lid Build-LC TEK [Re: stareatclouds]
#28631383 - 01/22/24 06:51 PM (5 days, 12 hours ago) |
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Nice Post here....loved the reading!
-------------------- Hoping to grow some great mushies with some TLC, Tender Loving Care!
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