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OfflineTheProfessor
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Registered: 01/06/04
Posts: 50
Last seen: 1 year, 15 days
Question on H2O2
    #2287795 - 01/30/04 08:00 PM (12 years, 10 months ago)

On 1-11 I inoculated 8 popcorn jars after 24h soak, 60min 15lbs... I kept 4 aside as a control. (Total 12 jars) One control was used to test the purity of the clean room and was left open the entire 1 hour I was werking in there.

Few days ago, out of curiosity I opened the clean room control and smelled it, rotten sour smell, bacillus.

On 1-28 I G2G one jar of popcorn that was done for about a week (The peroxide treated jar, I know this is still slow) to 10 WBS half gallons.

1-31 I injected 7 of 10 remaining quarts with 10ml of H2O2, and got ALOT of bubbling - then out of continuing curiosity I injected one control with H2O2, no bubbles. The remaining 3 were injected with 1ml H2O2.

The question is this - will a pure, uninfected sample of mycelium trigger the decomposition of H2O2?

On a side note - my cat will smell anything I hold out - he will smell normal jars a few times like he will smell anything - when his nose gets near an infected jar he pulls back. He can easily detect this thru the polyfil / tyvec, I cannot.

Prof


Edited by TheProfessor (01/31/04 01:04 PM)


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OfflineTheProfessor
newbie

Registered: 01/06/04
Posts: 50
Last seen: 1 year, 15 days
Re: Question on H2O2 [Re: TheProfessor]
    #2289277 - 01/31/04 10:16 AM (12 years, 10 months ago)

Pleeeeeeeease someone sacrifice some virgin mycelium to the cause of knowledge.... Pleeeeeeeeease!

Prof


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InvisibleMESCALATO
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Registered: 05/16/02
Posts: 1,025
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Re: Question on H2O2 [Re: TheProfessor]
    #2289295 - 01/31/04 10:45 AM (12 years, 10 months ago)

the other day took a agar plate of Ps. Azure that had a spot of infection around the rim of the petri, 3% h2o2 was poured directly on to the petri till it was full, no bubbling on the myc. but the infection bubbled like nuts.
does this help any
sry if it doesn't.


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OfflineTheProfessor
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Registered: 01/06/04
Posts: 50
Last seen: 1 year, 15 days
H2O2 - Bacterial detector? [Re: MESCALATO]
    #2289320 - 01/31/04 11:06 AM (12 years, 10 months ago)

Thanks mescalato I appreciate it, yes that seems to support my conclusion.

Anyone out there with a completely ininfected sample?

PM from anon...

...clean Ps. Azure did not bubble

There it is so now we can use H2O2 as a bacterial detector?

Prof


Edited by TheProfessor (01/31/04 01:03 PM)


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InvisibleJoshua
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Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Question on H2O2 [Re: TheProfessor]
    #2290583 - 01/31/04 10:21 PM (12 years, 10 months ago)

I differ...most fungi have enzymes (catalase, peroxidase) that break down H2O2. The result of which is the evolution of oxygen. I have often tested old cultures to see if they were still alive by cutting the culture and dripping a drop or two of peroxide in order to watch for the O2 bubbles. Many bacteria also produce peroxidases and catalases.

What you noticed may have just been a difference in the ability of each organism to produce these enzymes at the interface of peroxide.

Joshua


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Invisiblesimplemachine
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Registered: 09/14/03
Posts: 1,981
Re: Question on H2O2 [Re: TheProfessor]
    #2290648 - 01/31/04 10:57 PM (12 years, 10 months ago)

I agree with joshua on this one. I the the contam just bubbles MORE than the myc, but I'm sure the myc will also break down the H2O2.


SM


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