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Offlinemott
the crimsonavenger
Registered: 01/30/04
Posts: 28
Last seen: 12 years, 11 months
a little more info on substrate moisture
    #2287011 - 01/30/04 02:07 PM (13 years, 4 months ago)

my FOAF has been in the process of watching her 12 jars colonize for approximately one week and she's noticed a few things. first off, there is no fuzziness, no brilliantly white mycelium. what has appeared at the points of innoculation (in line with the innoc holes in the lids and dripping down the sides of her 1/2 pints) are copper colored streaks where the mycelium should be. like i said, its been a week at 76-80f, under the most sterile conditions she can achieve and in congruence with the PF tek (maximum flowering substrate). whats the deal? i've done some research and found some old threads that attribute this to excessive h2o, and apparently flipping the jars helps. but i've also read that you shouldn't flip the jars or remove the lids at all before 40% colonization. and of course there are those who colonize their jars without lids and advocate that method. she thinks its too moist. does that mean this batch it caput? *sigh*
a little help here guys!! thanks so much
mott


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Invisibleblazenaz
Extremist

Registered: 07/29/02
Posts: 309
Loc: Surrounded by an electrom...
Re: a little more info on substrate moisture [Re: mott]
    #2287040 - 01/30/04 02:19 PM (13 years, 4 months ago)

maybe rust dripping down from the holes in the jar lids? I would say up your temp a little bit and see if that helps.


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Blaze


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Offlinemott
the crimsonavenger
Registered: 01/30/04
Posts: 28
Last seen: 12 years, 11 months
Re: a little more info on substrate moisture [Re: blazenaz]
    #2287059 - 01/30/04 02:24 PM (13 years, 4 months ago)

rust?? is this normal?


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Invisibleblazenaz
Extremist

Registered: 07/29/02
Posts: 309
Loc: Surrounded by an electrom...
Re: a little more info on substrate moisture [Re: mott]
    #2287066 - 01/30/04 02:26 PM (13 years, 4 months ago)

Not the dripping part but all the metal jar lids rust within a few times of being PC'ed


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Blaze


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Offlinemott
the crimsonavenger
Registered: 01/30/04
Posts: 28
Last seen: 12 years, 11 months
Re: a little more info on substrate moisture [Re: blazenaz]
    #2287070 - 01/30/04 02:28 PM (13 years, 4 months ago)

well, this is their first trip in, though she did use an autoclave, but i don't see how that could have a negative impact. she's flipped the jars over, is that a bad idea when there has been no visible mycelium thus far? thanks for you help btw


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Invisibleblazenaz
Extremist

Registered: 07/29/02
Posts: 309
Loc: Surrounded by an electrom...
Re: a little more info on substrate moisture [Re: mott]
    #2287087 - 01/30/04 02:37 PM (13 years, 4 months ago)

If you moisture content is right, I don't think you have to flip. Most of the time, full colonization of half pints is about 1 to 2 weeks if your temp is 84F - 88F. Dude uses WBS and the only thing that seems to be needed is a shake. They usually never stall as long as moisture and temp is right and that would be your reasons for flipping. Also, tape is kept on holes until they are complete.


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Blaze


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Offlinemott
the crimsonavenger
Registered: 01/30/04
Posts: 28
Last seen: 12 years, 11 months
Re: a little more info on substrate moisture [Re: blazenaz]
    #2287095 - 01/30/04 02:42 PM (13 years, 4 months ago)

well i'd say that they're stalling wouldn't you? i'm almost sure theres no contam, they smell like any mushroom i've tripped before. the strain is huautla, and i can't seem to find any info on the colonization period for those little guys. apparently they're easy to cultivate. nothing has happened for about two days as far as anything noticable. flip em? what do you think?


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Offlinesmiley66
explorer
Registered: 12/19/03
Posts: 5
Loc: tx
Last seen: 13 years, 1 month
Re: a little more info on substrate moisture [Re: mott]
    #2287128 - 01/30/04 02:54 PM (13 years, 4 months ago)

Just wait afew more days and see what happens. Some strains colonize more slowly than others. If you think the h2o content is to high raise the temp. alittle.  If the jars arent atleast half colonized 2weeks after inoculation ,you got problem. and dont worry about flipping the jars its perfectly fine.The layer of vermiculite should hold back any contaminants trying to get in. Keep an eye on those streaks.good luck :mushroom2:


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Offlinemott
the crimsonavenger
Registered: 01/30/04
Posts: 28
Last seen: 12 years, 11 months
Re: a little more info on substrate moisture [Re: smiley66]
    #2289461 - 01/31/04 01:28 PM (13 years, 4 months ago)

she bumped the temp up to a constant 86.6 by throwing a rubbermaid tub on a register in her house!! of course a little h2o had to be added to the mix so the jars wouldn't dry out (and to regulate the heat). i'm so proud of her. three chears! Horah! does anyone know where the RH should be with colonizing jars? hers is between 75 and 80; seems a little high to me. let me know. thanks


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Offlinebeanchild
bean
Registered: 11/07/03
Posts: 158
Last seen: 12 years, 6 months
Re: a little more info on substrate moisture [Re: mott]
    #2289469 - 01/31/04 01:36 PM (13 years, 4 months ago)

next time go with eq's or b+ from ralphster, you'll see bright white mycillium in under 48 hours if you follow everything correctly!


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