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OfflineCrazy8
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Re: Stro's Agar Prep [Re: Stropharis]
    #22846889 - 01/31/16 02:56 PM (7 years, 11 months ago)

hey there! I was wondering instead of the whiskey bottle can I use a jar ?
btw nice write up!:wink:


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Invisiblemaddchef
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Re: Stro's Agar Prep [Re: Crazy8]
    #22846905 - 01/31/16 03:01 PM (7 years, 11 months ago)

I'm assuming you mean to cook in and pour out of.

Any autoclavable container will do if modified. Some are more user friendly than others


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblebodhisattaMDiscordReddit
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Re: Stro's Agar Prep [Re: maddchef]
    #22847246 - 01/31/16 04:46 PM (7 years, 11 months ago)

A dedicated media bottle is worth every penny. (7-10$ with shipping) last link in my signature.


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InvisibleStropharis
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Re: Stro's Agar Prep [Re: Crazy8]
    #22847959 - 01/31/16 07:41 PM (7 years, 11 months ago)

I'm using a one liter polypropylene erlenmeyer flasks in an AA921 nowahdays.


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OfflineCrazy8
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Re: Stro's Agar Prep [Re: Stropharis]
    #22851278 - 02/01/16 04:49 PM (7 years, 11 months ago)

i do have a erlenmeyer flasks but its a 500 ml and there no cap could it do the job if i put tyvek and foil on top of it or the cap is essential to the tek :confused:?


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InvisibleStropharis
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Re: Stro's Agar Prep [Re: Crazy8]
    #22853044 - 02/02/16 12:12 AM (7 years, 11 months ago)

Foil would do the trick.


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Invisibleelasticaltiger
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Re: Stro's Agar Prep [Re: Stropharis]
    #22853246 - 02/02/16 01:24 AM (7 years, 11 months ago)

Quote:

Stropharis said:
Working in a still air box space can be limited so I usually work in stacks of five dishes, sometimes ten depending on how drunk I am.





A-men to that.

Glad I'm not the only one.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

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Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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InvisiblebodhisattaMDiscordReddit
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Re: Stro's Agar Prep [Re: elasticaltiger]
    #22853794 - 02/02/16 06:58 AM (7 years, 11 months ago)

Likewise even open air 20 high stacks is hard to work with.


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OfflineSockadin
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Re: Stro's Agar Prep [Re: bodhisatta]
    #22853876 - 02/02/16 07:41 AM (7 years, 11 months ago)

I use a screw cap wine bottle. Most inexpensive white wine comes with screw caps now days instead of corks.

Although I have to use my Presto because My AA is a little to short for a 500ml wine bottle.

Lately I have been using a qrt mason jar to prep my agar. Just need to be careful when pouring plates so that you don't spill it everywhere.


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OfflineCrazy8
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Re: Stro's Agar Prep [Re: Sockadin]
    #22856491 - 02/02/16 06:50 PM (7 years, 11 months ago)

nice! thx for all the info guys!:grin:


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Invisibleelasticaltiger
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Re: Stro's Agar Prep [Re: bodhisatta]
    #22856667 - 02/02/16 07:31 PM (7 years, 11 months ago)

Quote:

bodhisatta said:
Likewise even open air 20 high stacks is hard to work with.




I meant glad I'm not the only one who pours plates drunk.

Quote:

Stropharis said:
I'm using a one liter polypropylene erlenmeyer flasks in an AA921 nowahdays.




Love those. I use one too. A sterile media flask is worth it's weight in gold.



--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Edited by elasticaltiger (02/02/16 07:35 PM)


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OfflineGreenRabbit
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Re: Stro's Agar Prep [Re: elasticaltiger]
    #22913527 - 02/16/16 08:30 PM (7 years, 11 months ago)

I tried a pour tek earlier and had the agar at the bottom become firm while the agar at the top was more watery.
Any tips on how to avoid this?
It was all the same consistency before going into the PC.


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InvisiblebodhisattaMDiscordReddit
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Re: Stro's Agar Prep [Re: GreenRabbit]
    #22913534 - 02/16/16 08:33 PM (7 years, 11 months ago)

Quote:

GreenRabbit said:
I tried a pour tek earlier and had the agar at the bottom become firm while the agar at the top was more watery.
Any tips on how to avoid this?
It was all the same consistency before going into the PC.



I've never seen that happen. I would have to see it to believe it. in the dishes or in the bottle you sterilized the agar in?


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OfflineGreenRabbit
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Re: Stro's Agar Prep [Re: bodhisatta]
    #22913544 - 02/16/16 08:35 PM (7 years, 11 months ago)

In the bottle, I used a quart jar, and allowed the PC to cool for an hour before taking the jar out.


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InvisibleStropharis
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Re: Stro's Agar Prep [Re: GreenRabbit]
    #22913780 - 02/16/16 09:48 PM (7 years, 11 months ago)

Pour hotter, the bottom agar is cooling too much before your pour is complete.


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OfflineGreenRabbit
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Re: Stro's Agar Prep [Re: Stropharis]
    #22914055 - 02/16/16 11:11 PM (7 years, 11 months ago)

I figured.. Just saw some people say to wait a few hours to avoid condensation on petris. I'll try to do it as soon as I can touch the PC I guess.
Thanks.


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OfflineCrazy8
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Re: Stro's Agar Prep [Re: GreenRabbit]
    #22916461 - 02/17/16 04:58 PM (7 years, 11 months ago)

i let it cool down 15 min and its perfect a bit condensation but very small maybe wait a 20 min :grin:


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Invisibleelasticaltiger
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Re: Stro's Agar Prep [Re: GreenRabbit]
    #22925096 - 02/19/16 11:45 PM (7 years, 11 months ago)

Quote:

GreenRabbit said:
I figured.. Just saw some people say to wait a few hours to avoid condensation on petris. I'll try to do it as soon as I can touch the PC I guess.
Thanks.




If you double up on nitrile gloves it buys you some good time. You can pour in sets of 5 or so and give your hand a few seconds to cool down before you grab it again.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Edited by elasticaltiger (02/19/16 11:45 PM)


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OfflineGreenRabbit
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Re: Stro's Agar Prep [Re: elasticaltiger]
    #22926792 - 02/20/16 02:15 PM (7 years, 11 months ago)

Oh I don't think I'll have a problem holding a hot jar. I can't feel shit through my fingertips due to calluses from playing guitar and bass.
I just saw some posts about letting the agar cool until it is about to set to avoid condensation. Guess that was bad advice...


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InvisiblebodhisattaMDiscordReddit
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Re: Stro's Agar Prep [Re: GreenRabbit]
    #22926799 - 02/20/16 02:18 PM (7 years, 11 months ago)

https://www.shroomery.org/forums/showflat.php/Number/21922023


You want to cool your agar in a water bath then pour.
Quote:




Why 47?

Quote:

Agar exhibits hysteresis, melting at 85 °C (358 K, 185 °F) and solidifying from 32–40 °C (305–313 K, 90–104 °F). This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures.



I find it starts to gel back up at about 107F or 42C most of the time at the gel strength used for plates. at 47C you can pour a whole sleeve of plates before it gels up on you. and pouring at as low of a temperature as possible minimizes condensation a lot.

to get agar to melt again you have to go all the way back up to 85C, once it is gel simply heating it 47+ won't melt it.





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