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OfflineShamanic Scientist
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An exercise in patience - my third cubensis monotub
    #22846463 - 01/31/16 12:58 PM (7 years, 11 months ago)

Hey guys,

I am posting some pictures of a cubensis monotub I recently out into fruiting conditions. This grow has been sort of interesting in different ways. First off, I am using a standard 50/50 coir+verm tek. Second, the mycelium that went into my monotub was composed of two types: about half came from MS and the other half from a mushroom I cloned from my previous grow.

I had previously decided to try my hand at cloning. However, due to time constraints I can't clone properly by getting into agar and carefully selecting strains for propagation. I simply grabbed a fruit I liked, swabbed it with alcohol, and took a sample with a syringe that I then grew up in a honey LC.

Once I had a good LC, I shot some into rye jars. as luck would have it, this strain was slow growing, and did not have the most vibrant growth (either in terms of color, which was off-white, or in terms of physical form). The mycelium was somewhat "diffuse" in it's general appearance. Unfortunately I didn't take pictures of my jars.

Because the mycelium from my LC was slow-growing, I also grew up some jars from MS. I picked a few of my best looking jars, and mixed all of this together with my cloned mycelium from the other jars when I made the monotub.

First thing I noticed with this tub: it was SLOW. I was pretty worried for a while that I had contamination that would force me to throw out the tub. Now, there were no signs of contamination other than slow growth, although there was a smallish patch near the middle of my substrate that looked uncolonized for some time. However, as you will see, this patch did eventually start to fill in, especially with knots after I introduced fruiting conditions.

Just over one month after I made the tub, I decided to introduce it into fruiting conditions. This was despite the fact that things were not fully colonized yet. I was worried that the tub wasn't all that healthy, so I initiated fruiting just to cut my losses and hopefully get a small number of fruits from it.

I introduced fruiting conditions ~one week ago, and started misting and fanning two days ago. My this point I already had lots of knots, and today I have my first clear pin. See pictures below.




Here's a fairly zoomed out shot. This is several days after I introduced fruiting conditions. A couple of things I want to point out. First, there are lots of knots. Second, you will notice that there clearly seem to be different strains growing. This is not surprising but it's striking how obvious it is. Some places (e.g. bottom-right corner) have really solid white, filled in patches of mycelia. Other places are more spotty, and clearly do not look fully colonized. My suspicion is that the more solid-looking areas are places where mycelia from my MS jars are growing, while the "weaker" looking areas are from my clone. Nonetheless, I was getting knotting, and I got way more after fruiting conditions were initiated.




Closer shot. Notice knots, and areas with solid-looking growth, including the little "mountain" where a chunk of substrate is poking up into the air, which is nicely colonized.




Another shot of the bottom-right corner of my tub. This area has some of the healthier-looking growth. One thing I also notice is that my areas with more solid growth have fewer knots, while the spottier areas of the tub exploded with knots.




This is an area that was slower to colonize, but exploded with knots, especially after fruiting and after I started misting+fanning two days ago. My first clear pin is visible near the center of the image.




One more shot for good measure.


Feel free to discuss anything you find interesting! I guess one lesson I hope people newer to growing can take from this is that sometimes all you need is patience. This tub took much longer to colonize than my previous tubs, but in the absence of overt signs of contamination, just giving the monotub time looks like it paid off. Again, this tub was started over a month ago (~5 weeks), and has been in fruiting conditions for like 5 days now.


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OfflinecronicrFacebook
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Re: An exercise in patience - my third cubensis monotub [Re: Shamanic Scientist]
    #22846538 - 01/31/16 01:14 PM (7 years, 11 months ago)

i would tighten up your bottom holes, it's looking dry.


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OfflineShamanic Scientist
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Re: An exercise in patience - my third cubensis monotub [Re: cronicr]
    #22846570 - 01/31/16 01:22 PM (7 years, 11 months ago)

Yes, I think you're right, although my poly is packed relatively tight. I have been misting more since this morning.


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Re: An exercise in patience - my third cubensis monotub [Re: cronicr]
    #22846577 - 01/31/16 01:27 PM (7 years, 11 months ago)

Quote:

cronicr said:
i would tighten up your bottom holes, it's looking dry.




:whathesaid: Stop fanning.  The only thing I consider fanning for monos in a dry climate is when you open the lid to mist. Kill any fans you may have going as well.


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OfflineShamanic Scientist
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Re: An exercise in patience - my third cubensis monotub [Re: Pastywhyte]
    #22847000 - 01/31/16 03:23 PM (7 years, 11 months ago)

Sounds good. I now simply have a small heater in my grow room in order to keep the room in the low 70s (previously I had an air filter on in the same room, away from the tub).


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OfflineShamanic Scientist
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Re: An exercise in patience - my third cubensis monotub [Re: Shamanic Scientist]
    #22858478 - 02/03/16 07:11 AM (7 years, 11 months ago)

Here's what my pins are looking like as of this morning:




Looks like the early ones are a bit on the short and wide side.


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InvisiblePastywhyteMDiscord
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Re: An exercise in patience - my third cubensis monotub [Re: Shamanic Scientist]
    #22858523 - 02/03/16 07:39 AM (7 years, 11 months ago)

Looks good. Short plump pins like that are indicative of good FAE.


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OfflinePsilosoulful
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Re: An exercise in patience - my third cubensis monotub [Re: Shamanic Scientist]
    #22858653 - 02/03/16 08:31 AM (7 years, 11 months ago)

Quote:

Shamanic Scientist said:
Here's what my pins are looking like as of this morning:




Looks like the early ones are a bit on the short and wide side.



Looks good, lots of knots, that tub should take off soon! :sun:


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OfflineShamanic Scientist
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Re: An exercise in patience - my third cubensis monotub [Re: Pastywhyte]
    #22860697 - 02/03/16 06:19 PM (7 years, 11 months ago)

Thanks for the input. What do you think about the differences between how things look in the central area of heh tub vs. the periphery? I have three shots below that tile one end of my tub. You can use the largest pin as an anchor point. Basically, in the central area I've got pins and a ton of knots. In contrast, growth in the corners has no pins, many fewer knots, and a different overall appearance. What's a bit puzzling to me is that the corners/sides seemed to haver stronger mycelial growth during the colonization phase: the surface colonized more quickly and fully, and the mycelium looked nice and white, and covered the surface. Nearer to the middle, where I now see pins an knots, the surface never fully colonized. You can see this clearly by looking at where my pins and knots are currently - the surface is not fully covered by mycelium. The corners/sides also seem to have lost some of the brilliant whiteness I observed during colonization, and now appear more off-white or "dull" in color. Thoughts?










Edited by Shamanic Scientist (02/03/16 06:20 PM)


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Re: An exercise in patience - my third cubensis monotub [Re: Shamanic Scientist]
    #22860962 - 02/03/16 07:25 PM (7 years, 11 months ago)

It's dry.


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OfflinePsilosoulful
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Re: An exercise in patience - my third cubensis monotub [Re: Pastywhyte] * 1
    #22860984 - 02/03/16 07:30 PM (7 years, 11 months ago)

He's right, you need to mist it more until the surface is glistening, then re-mist when it dries up.



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Re: An exercise in patience - my third cubensis monotub [Re: Psilosoulful]
    #22861059 - 02/03/16 07:44 PM (7 years, 11 months ago)

Quote:

Psilosoulful said:






:lmafo:


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OfflinePsilosoulful
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Re: An exercise in patience - my third cubensis monotub [Re: psylo-not]
    #22861075 - 02/03/16 07:50 PM (7 years, 11 months ago)

:smile:


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OfflineShamanic Scientist
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Re: An exercise in patience - my third cubensis monotub [Re: Psilosoulful]
    #22861184 - 02/03/16 08:20 PM (7 years, 11 months ago)

:thumbup:


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Re: An exercise in patience - my third cubensis monotub [Re: Shamanic Scientist]
    #22861220 - 02/03/16 08:28 PM (7 years, 11 months ago)

:underageban:

That's a regular gremlin now psilo. "underageban".


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OfflinePreparationH
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Re: An exercise in patience - my third cubensis monotub [Re: Pastywhyte]
    #22861389 - 02/03/16 08:56 PM (7 years, 11 months ago)

Quote:

Pastywhyte said:
Quote:

cronicr said:
i would tighten up your bottom holes, it's looking dry.




:whathesaid: Stop fanning.  The only thing I consider fanning for monos in a dry climate is when you open the lid to mist. Kill any fans you may have going as well.



Why kill the fan?  Because of a dry climate or you dont like running a fan at all?  My mini has been drying out but I mist it daily to fight it.  Remove my fan I guess?


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Re: An exercise in patience - my third cubensis monotub [Re: PreparationH]
    #22861419 - 02/03/16 09:03 PM (7 years, 11 months ago)

IMO fans are best suited to humid climates in a set and forget style mono. Most people do not stuff their bottom holes tight enough and combine that with furnaces or AC drying the air out, and fan is going to do you few favors. Of course its specific to your individual house but I never run a fan, if I did it would dry everything right out. The sub in most monos is going to move plenty of air on their own just from the heat they generate. Fan is overkill most of the time.


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OfflinePreparationH
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Re: An exercise in patience - my third cubensis monotub [Re: Pastywhyte]
    #22861471 - 02/03/16 09:17 PM (7 years, 11 months ago)

Shit I think that's my issue then.  I was wondering why everyone keeps saying monos can be set and forget yet I have to mist every day.


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OfflinePsilosoulful
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Re: An exercise in patience - my third cubensis monotub [Re: PreparationH]
    #22861501 - 02/03/16 09:25 PM (7 years, 11 months ago)

How tight should those bottom holes be stuffed. Is there a tek for that?
I'm always guessing on it, and end up going either too tight or too loose :shrug2:


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Re: An exercise in patience - my third cubensis monotub [Re: Psilosoulful]
    #22861515 - 02/03/16 09:27 PM (7 years, 11 months ago)

Seek the doughnut of dryness.


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