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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: Gr0wer]
#22832311 - 01/27/16 09:53 PM (8 years, 3 months ago) |
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Quote:
Gr0wer said: You would need to let the whole PC cool in the SAB. When the PC equalizes air rushes in and can contaminate the dishes. Last week i made a lazy batch of dishes and allowed the PC to cool without the flow hood and i got some wild myc growing
You really don't need to do all that bullshit to get uncontaminated glass dishes. They're all I used before I got styrene dishes for free. Never once let the PC cool down anywhere but right on the stove. Fuck the dishes came out of the PC while its on the stove. I don't even take the PC to the same room the SAB is in
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Gr0wer
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: bodhisatta]
#22832651 - 01/28/16 12:12 AM (8 years, 3 months ago) |
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Well it's all case by case. Your stove/kitchen area might be clean. My lab is inconveniently located in my garage along with 30 lbs of mushrooms a week fruiting all around it with shitloads of FAE. Ive already tried cooling with no precautions and no bueno as mentioned, lost 1/2 the plates in less than a week, mostly mushroom myc. PC'ing inside the house is not an option at this point, the wife already puts up with enough of my shenanigans.
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bodhisatta 
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: Gr0wer]
#22833081 - 01/28/16 06:37 AM (8 years, 3 months ago) |
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Do you have them in an autoclave bag or wrapped in foil? That's probably your problem.
I've got flasks at work so big they don't fit in my 23q presto or the clave at work. They get brought home and oven sterilised then go back in my car and still don't contaminate its about the preparations
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mushpunx
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: bodhisatta]
#22833238 - 01/28/16 07:42 AM (8 years, 3 months ago) |
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Just wrap the plates in foil or seal them up in a spawn bag and then pour them like they were disposables
You also might consider converting your PC into a sterilizer (if its an AA) You just switch the black rubber plug for a red high pressure on and unscrew the valve the rocker weight sits on and screw in the stop cock valve.
Its like 30$ tops for the parts, I could never go back. Then you wouldnt have to wrap your plates you just break the vacuum by opening the valve in front of a flowhood and move the plates in front of the hood.
Do you like the glass/do you have a lot of them? I really wouldnt mind having some but Id need a couple sleeves realistically. Like 50 or so. I could use them for germination plates and ones that are just gunna need to be transferred , would really save on disposables. I like PP5s a lot but They're hard to see into with the lids on
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 Amateur Mycologists United AMU Q&A
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Gr0wer
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: mushpunx]
#22833275 - 01/28/16 07:51 AM (8 years, 3 months ago) |
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I like them. I ended up getting the cheapos off ebay, they were like $2 each from china. I belive they are flint glass and not borosilicate but they seem to hold up well, its not like im tossing these things around the lab. I like the idea of using a growbag, that and ultimately the autoclave conversion, that will have to wait as i already spent beyond my limit this month in upgrades to my pasteruizer/steamer ric.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: Gr0wer]
#22833565 - 01/28/16 09:37 AM (8 years, 3 months ago) |
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I've got 4cases 48 pyrex dishes. I absolutely hate using them I would buy sleeves of styrene disposables even if they were 10$ a sleeve to not have to dick with the glass dishes. But you definitely don't need to cool your PC down in a SAB or FH
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mushpunx
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: bodhisatta]
#22833668 - 01/28/16 10:16 AM (8 years, 3 months ago) |
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That bad? What exactly is it you dislike About them?
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 Amateur Mycologists United AMU Q&A
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bodhisatta 
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: mushpunx]
#22833899 - 01/28/16 11:22 AM (8 years, 3 months ago) |
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Slippery when stacked even if they're bone dry. The lids fit like shit because they have to. You have to sterilize them, people get worried about recycling plastic the environment and shit but fucking really? It takes more energy to sterilize glass than its worth to buy disposable. Besides compared to an average humans polution petris are negligible. They're not exactly machine flat they're a little bit bumpy. They take up more space. You have to clean them immaculately to sterilize them for re use
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laughingdog
Stranger

Registered: 03/14/04
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: Kizzle]
#22834015 - 01/28/16 12:08 PM (8 years, 3 months ago) |
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Quote:
Kizzle said: ..... They'll be fine sitting there while they cool I give them a couple hours.
Also in case you didn't know you can oven sterilize glass petris. That's the main advantage over the reusable polypropylene petris. -------------------- quote 2
Cleaning out the used dishes is extremely easy. I just scrape any leftover agar into the toilet. If the dishes were contaminated with mold place the still wrapped dish in the microwave and heat it, should only take 10-20 seconds, until the agar has completely liquified, let it resolidify and that should destroy most of the spores (actually destroyed all the spores in all the tests I've done) and trap what is left in the agar so you can clean it out without releasing them into the air.
so can dry glass petridishes be sterilized with a microwave & skip the oven and PC?
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Gr0wer
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: laughingdog]
#22834047 - 01/28/16 12:14 PM (8 years, 3 months ago) |
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use the oven, not microwave.
With stacking i go 4 high and use xl rubberbands to hold em together. They are really supposed to be held in racks. I only have 3-8 plates growing out normally so its not too bad imo. All i use agar for is growing out masters. If was making isolates or cleaning up something contaminated that would be another story. And its not about the energy, it's about the waste. I highly doubt any companies recycle dirty pietris. My spawn production is container waste free, glass dishes to quart jars. And i produce 18-36 lbs of spawn a week like this.
Edited by Gr0wer (01/28/16 12:16 PM)
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laughingdog
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: Gr0wer]
#22834437 - 01/28/16 02:13 PM (8 years, 3 months ago) |
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Quote:
Gr0wer said: use the oven, not microwave. .... And its not about the energy, it's about the waste.
Thanks! 'about the energy', think I'll get a toaster over that goes to 450º
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mushpunx
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: laughingdog]
#22834618 - 01/28/16 03:10 PM (8 years, 3 months ago) |
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I throw out way more worse shit than pitres. Really its not the waste that bothers me its the cost
I mean theyre pretty cheap by the case but I still burn through them fast. Its nice to have something reuseable to throw in the mix, especially when I know Im just gunna be making a transfer anyways
But I think Ill stick with PP5 for that
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bodhisatta 
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: mushpunx]
#22834809 - 01/28/16 04:01 PM (8 years, 3 months ago) |
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I would much rather use glass dishes than pasty plates. No offense to pasty but I don't live in Africa(or Canada because they can't get dishes cheap either)
Also on the topic of oven sterilization look up the wiki article on dry heat sterilization before you throw glass into a 450f toaster oven
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mushpunx
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: bodhisatta]
#22834867 - 01/28/16 04:21 PM (8 years, 3 months ago) |
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Dude you made me picture that Sally lady from the 90s on TV, trying to gather up donations of pitre plates for cultivators in Mogadishu
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laughingdog
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: bodhisatta]
#22835015 - 01/28/16 05:08 PM (8 years, 3 months ago) |
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Quote:
bodhisatta said: ....
Also on the topic of oven sterilization look up the wiki article on dry heat sterilization before you throw glass into a 450f toaster oven
Thanks ---- looked it up ---seems
... 170 °C (340 °F) for 1 hour in a convection oven, would be better
still a counter top convection oven should use less power than a full size stove oven
from wiki
"The proper time and temperature for Dry-Heat sterilization is 160 °C (320 °F) for 2 hours or 170 °C (340 °F) for 1 hour.[citation needed] Instruments should be dry before sterilization since water will interfere with the process."
"A mechanical convection oven contains a blower that actively forces heated air throughout all areas of the chamber. The flow created by the blower ensures uniform temperatures and the equal transfer of heat throughout the load. For this reason, the mechanical convection oven is the more efficient of the two processes."
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: laughingdog]
#22835309 - 01/28/16 06:08 PM (8 years, 3 months ago) |
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it never hurts to go a little longer either.
remember too if your shit says pyrex it might not be actual borosillicate glass it might be soda lime, as the "made great in america" actually means we're the only country in which pyrex isn't made from pyrex.
borosilicate glass can withstand before fracturing is about 165.56 °C (330 °F). This compares well with soda lime glass, which can withstand only a 37.22 °C (99 °F) change in temperature
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elasticaltiger
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Registered: 06/24/13
Posts: 8,438
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: bodhisatta]
#22835416 - 01/28/16 06:29 PM (8 years, 3 months ago) |
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Wow I just read a fuck ton on wiki about sterilization and related practices. Thanks bodhi.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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elasticaltiger
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Registered: 06/24/13
Posts: 8,438
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: elasticaltiger]
#22835431 - 01/28/16 06:31 PM (8 years, 3 months ago) |
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"For autoclaving, cleaning is critical. Extraneous biological matter or grime may shield organisms from steam penetration. Proper cleaning can be achieved through physical scrubbing"
wiki
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: elasticaltiger]
#22835461 - 01/28/16 06:37 PM (8 years, 3 months ago) |
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Quote:
elasticaltiger said: "For autoclaving, cleaning is critical. Extraneous biological matter or grime may shield organisms from steam penetration. Proper cleaning can be achieved through physical scrubbing"
wiki
like the grain itself, hence why everything else but grain sterilizes in less than 30m at 15psi.
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Gr0wer
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Re: Is a no-pour agar method with glass petri dishes a good idea? [Re: bodhisatta]
#22835860 - 01/28/16 08:10 PM (8 years, 3 months ago) |
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quick question, so after reading up on the autoclave conversion it seems the stopcock is the mod. Now i run my PC automated off a PID, it sounds like the stopcock i would need to manually flipped? is this sort of like leaving your rocker off to purge? With my rocker i place it on the PC while cold and allow the PID to purge the air with a higher setting for 10 min, would this function work the same with a stopcock?
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