Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Mono Tub Substrate   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate

Jump to first unread post Pages: < First | < Back | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | Next >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineharrb
Shroomepreneur

Registered: 10/22/04
Posts: 20
Loc: Alabama
Last seen: 18 years, 9 months
Re: The Coffee Project [Re: RogerRabbit]
    #3282073 - 10/26/04 10:29 PM (19 years, 8 months ago)

Have you had any experience with azures?  Any adivce would help, cause I'd hate to think I've put this much work into it just to see all my cakes fail.

harrb :mushroom2:


--------------------
Everything I need... leave it to the trees... it can make me feel better!

Extras: Filter Print Post Top
InvisibleMmkay
newbie
Registered: 05/12/02
Posts: 47
Re: The Coffee Project [Re: harrb]
    #3287521 - 10/27/04 10:50 PM (19 years, 8 months ago)

I just did 12 BRF 1/2 pint jars tonight with diluted coffee. I'll update when I have more info.

Extras: Filter Print Post Top
Offlinedaimyo
Monticello

Registered: 05/13/04
Posts: 7,751
Last seen: 12 years, 4 months
Re: The Coffee Project [Re: daimyo]
    #3310505 - 11/02/04 05:07 PM (19 years, 7 months ago)

My project will no longer be updated. It's not a failure, but due to off water content the results are useless.

Will be trying again soon.


--------------------
"I have sworn upon the altar of God eternal hostility against every form of tyranny over the mind of man."

Extras: Filter Print Post Top
OfflineAnArKey
newbie
Registered: 11/26/01
Posts: 36
Last seen: 18 years, 8 months
Re: The Coffee Project [Re: daimyo]
    #3314932 - 11/03/04 12:57 PM (19 years, 7 months ago)

Wouldn't it be great if we could get azures to invitro? With cloned tissue, coffee, and a trip to the refrigerator, it could happen.

Here's what I think you should try harrb. One jar of grain/coffee with azures, and another with wood chips/coffee. Once fully colonized, sit for another week. Put in fridge, but disable your switch so the light stays on. If nothing happens in 2 weeks like that, the jars are still ok, nothing lost. With any luck, you may get a pin or two. Those pins would be the perfect tissue to clone, and repeat this process. Next time around, good chance you have a jar full of azures.

Edited by AnArKey (11/03/04 01:01 PM)

Extras: Filter Print Post Top
Offlineharrb
Shroomepreneur

Registered: 10/22/04
Posts: 20
Loc: Alabama
Last seen: 18 years, 9 months
GOOD NEWS FOR AZURES!!! [Re: AnArKey]
    #3328836 - 11/06/04 09:08 PM (19 years, 7 months ago)

I got great news for all those trying to improve mycelium growth rate for azures!  I asked Roger if he had tried the coffee project with azures, and he wasn't sure, so I did the test myself.  I took 2 1/2pint cakes that had spent 3 weeks growing nothing in my terrarium, and a 1/4 pint of crumbled cake from another experiment that I didn't use.  I dunked all three cakes in 50/50 water/coffee for one day at room temp.  At the same time, I had a big bowl of hardwood chips (more like mulch) that I got from Lowe's.  I mixed some vermiculite in there (about 3/8 as much as the mulch content), a little bit of previously sterilized top soil, and a little bit of brown rice flour.  I poured 50/50 coffee/water into that mix as well and let it set for a day. The next day (Thursday), after everything had a day to soak in coffee-water, I took everything out.  I took one of those Kitchen-Aid grinders that have the two spinning blades, sliced the cakes into pieces that would fit into the machine, then grinded the hell out of them.  I drained the mulch mix of the coffee-water for about 10 minutes, then placed them in a rubbermaid container with lid that was about the size of a shoebox.  Then I sprinkled the ground mycelium over it and mixed real good.  I put the lid on the container and put it in a dark place to sit at room temp.  I checked it tonight (Saturday - two days later) and the mycelium is spreading like wildfire, no joke.  I have no doubt that it will take over that entire container within the next 5 days.  So yes, coffee works EXTREMELY well with Azures.  Also, in case you were wondering, I did not sterilize the woodchip/verm/brf mix, more like pasturized them with the hot coffee.  I don't think sterilization here is a big issue because the coffee is making the mycelium so fast, that the container would be completely colonized before any infection could set in.  But... I did make sure the room I mixed everything in was lysoled, so as to not infect it from the beginning.  Give it a shot peeps, maybe if there are more people successful at getting the chips colonized indoors, we can work together to get the fruiting process right.  I'm working on a method right now that I feel very confident in, but I'm going to test it before I start posting anything.  One more thing, you should poor the hot coffee mix into the mulch mix bowl, but not when dunking the cakes.  Make sure the coffe-water has cooled down to room temp before dunking them, and when you do, make sure the coffee-water completely submerges the cakes.  Thank you to Hippie3 for the coffee dunkin idea.  I give him full credit for the idea, but I'll take a little for doin it with azures  :thumbup:.  His procedure can be found at... http://www.mycotopia.net/discus/messages/5/103314.html?1075388611

harrb :mushroom2:


--------------------
Everything I need... leave it to the trees... it can make me feel better!

Extras: Filter Print Post Top
OfflineIGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 1 year, 1 month
Re: The Coffee Project [Re: harrb]
    #3333526 - 11/08/04 01:54 PM (19 years, 7 months ago)

wow thats amazing, gl with the project and others.damn...keep it up

Extras: Filter Print Post Top
Offlineticktock
Seeker
 User Gallery

Registered: 10/02/04
Posts: 389
Loc: Just ask the rabbit.
Last seen: 14 years, 3 months
Re: The Coffee Project [Re: IGnosticAbhorI]
    #3340874 - 11/10/04 10:07 AM (19 years, 7 months ago)

I just read this whole thread. Lots and lots of sizzle, but not much steak. Several people have posted the start of good, valid controlled experiments. "I'll report back in a few days." is a typical promise. Then the reports don't come.

Conclusion;
People who do the experiment die. :shocked:

JK of course.

The right way to do this is to have a control group and an experimental group. There must be only one difference between the 2 groups or the results are meaningless. IMHO we need to focus on 50% brewed coffee in the jars only. Forget about grounds, tea, coke, mountain dew, caffeine pills, etc for now. Let's see if we can get repeatable, predictable results and save all the other stuff for future experiments.

I'd do it myself, but can't just now. Besides, I don't want to die  :wink:


--------------------
Don't panic!

Extras: Filter Print Post Top
OfflineSilven
Male

Registered: 08/30/04
Posts: 2,072
Loc: El Mexicano
Last seen: 11 months, 11 days
Re: The Coffee Project [Re: ticktock]
    #3341031 - 11/10/04 10:49 AM (19 years, 7 months ago)

I wrote earlier:
Quote:

** CONTROL GROUP **

First 6 jars prepared:

3 1/2 cups vermiculite
3/8 cups brown rice flour
1 7/8 cups water

Everything was mixed together, added to jars, covered with a dry layer of vermiculite, lids applied, two layers of foil added, and boiled slowly for 1 hour from the beginning of the boil.

Innoculations:

2 jars will be inoculated and labeled equadorian
4 jars will be inoculated and labeled Thai Super Strain


** COFFEE GROUP **

6 more jars prepared:

3 1/2 cups vermiculite
3/4 cups brown rice flour
1 cup water
7/8 cup coffee

Again everything was mixed in a clean bowl. I added the mixed substrate to 6 jars and applied a dry layer of vermiculite to each, put on the lids, covered with two layers of foil and placed in a seperate pan to boil slowly for an hour.

Today when I inoculate:

I will inoculate 2 jars with equadorian and label accordingly and
I will inoculate 4 jars with Thai Super Strain and label

-------------------------------------------------------------------

All of these jars will be incubated together in my incubation chamber that is kept between 80 and 85 degrees.





So the equadors were basically even.

The thai super strain on the other hand colonized the coffee added substrate much quicker. I'd say that it was done around 8 days faster than the other jars which took up to 20 days to fully colonize.

I will also be trying this with rye grain soon.

The results are definately duplicateable if you know what you are doing and don't continue to compound more experiments on top of the one at hand (coke and all that shit added, etc.)

Anyway, I am impressed with the results from coffee and as Rodger said in his other posts, the results have already been duplicated multiple multiple times. It's now a matter of determining what it is about coffee that makes it grow so much faster, rather than continuing to duplicate his methods and results.

Any suggestions on where to go from here? I highly doubt it's the caffeine, rather the nutes that are making it grow so fast, but that's just my opinion.


--------------------
What do you bring to the table?

Extras: Filter Print Post Top
Offlineticktock
Seeker
 User Gallery

Registered: 10/02/04
Posts: 389
Loc: Just ask the rabbit.
Last seen: 14 years, 3 months
Re: The Coffee Project [Re: Silven]
    #3341282 - 11/10/04 11:41 AM (19 years, 7 months ago)

Thanks, Silven

Did you see a difference in yields, or just colonization speed?


--------------------
Don't panic!

Extras: Filter Print Post Top
OfflineMycoJunkie
Psilanthropist

Registered: 11/04/04
Posts: 963
Loc: .4merica
Last seen: 18 years, 5 months
Re: The Coffee Project [Re: ticktock]
    #3359755 - 11/14/04 12:16 PM (19 years, 7 months ago)

Omfg.

Coffee grounds here I come.

However, isn't coffee acidic? Wouldn't it ruin the pH? Hmm..

Edited by MycoJunkie (11/14/04 12:31 PM)

Extras: Filter Print Post Top
Offlinebigslick
science fictionwriter

Registered: 02/16/02
Posts: 186
Loc: just behind you
Last seen: 11 years, 9 months
Re: The Coffee Project [Re: Hippie3]
    #3363074 - 11/15/04 07:38 AM (19 years, 7 months ago)

Just thought I could add to this thread a bit...

Posted on Tuesday, May 18, 2004 - 07:43 pm: at www.Mycotopia.com

--------------------------------------------------------------------------------
Let the eq cake go a bit too long and it sporalated a little. Tc pint cake(cake on the right in the jar) made with a little bit of coffee and coffee seems to help with invitro pinning. Other tc pint cakes made at same time are way behind. In general the tc's don't do as well invitro as my gt's or eq's have done.



--------------------


Everything I write is a total work of fiction

Extras: Filter Print Post Top
Invisiblederx
who run it
 User Gallery

Registered: 05/29/03
Posts: 2,459
Loc: dx/dt
Re: The Coffee Project [Re: bigslick]
    #3363251 - 11/15/04 09:27 AM (19 years, 7 months ago)

well fuck all the bullshit. I soaked grains for 8 hours, added about half coffee for 4 additional hours and am simmering with coffee water. I hope to see a difference.


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.

Extras: Filter Print Post Top
OfflineSilven
Male

Registered: 08/30/04
Posts: 2,072
Loc: El Mexicano
Last seen: 11 months, 11 days
Re: The Coffee Project [Re: ticktock]
    #3363432 - 11/15/04 10:29 AM (19 years, 7 months ago)

Ticktock, I didn't see much difference in yield, perhaps there would have been a greater margin if I would have used grain instead of BRF, but yea the colonization time definately went down on the ones with coffee compared to the ones without.  :thumbup:

Sorry for the 5 day late response.


--------------------
What do you bring to the table?

Extras: Filter Print Post Top
Offlineticktock
Seeker
 User Gallery

Registered: 10/02/04
Posts: 389
Loc: Just ask the rabbit.
Last seen: 14 years, 3 months
Re: The Coffee Project [Re: Silven]
    #3364960 - 11/15/04 04:26 PM (19 years, 7 months ago)

Thanks Silven,

What I've gotten from this is that coffee;
1 May speed colonization.
2 Wont hurt, unless it's used in casing media.

That about right?


--------------------
Don't panic!

Extras: Filter Print Post Top
Offlineamyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 10 years, 5 months
Re: The Coffee Project [Re: ticktock]
    #3370144 - 11/16/04 05:33 PM (19 years, 7 months ago)

there also seems to be evidence to suggest that coffee also may increase pinning speed and abundance. at the very least with in vitro methods.


--------------------
"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein

Extras: Filter Print Post Top
OfflineRogerRabbitV
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 months
Trusted Cultivator
OG Cultivator
Re: The Coffee Project [Re: amyloid]
    #3392553 - 11/21/04 08:49 AM (19 years, 7 months ago)

Definitely increases pinning invitro, PROVIDED you have an invitro capable isolate. Some strains/substrains will not pin invitro at all, but will pin normally in a casing.

To sum up 40 pages, coffee will help speed colonization of whole grains. The jury is still out on its effectiveness with pf cakes. It will help speed up the pinning process. It will not appreciably increase total yield in p. cubensis. The morning coffee grinds added to the casing peat/verm will also help to speed up pinning. I've proved this with side-by-side experiments. Run your own tests if you doubt it.

Perhaps the biggest WOW for coffee in my opinion is the dramatic increase in speed and total yield of sclerotia producing species when coffee is used to hydrate the grains. I've used it with popcorn, rye and rye grass seed with better results than plain water.

While on the subject of sclerotia producing species, my tests also show that rye berries outperform rye grass seed. Popcorn doesn't even come close to either one.


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
InvisibleGRTUD
INFP
Male

Registered: 01/30/01
Posts: 270
Loc: United States
Re: The Coffee Project [Re: Hippie3]
    #3393352 - 11/21/04 03:26 PM (19 years, 7 months ago)

Have you ever noticed how fast mold grows on the surface of old coffee (like have you ever gone away for a holiday or a long weekend and forgotten to empty the coffee maker)? This sounds very promising! "Yummmm, coffee"


--------------------
"New shit has come to light..."

Edited by GRTUD (11/21/04 03:45 PM)

Extras: Filter Print Post Top
Offlineharrb
Shroomepreneur

Registered: 10/22/04
Posts: 20
Loc: Alabama
Last seen: 18 years, 9 months
Re: The Coffee Project [Re: RogerRabbit]
    #3407922 - 11/25/04 11:41 AM (19 years, 7 months ago)

Quote:

RogerRabbit said:
Definitely increases pinning invitro, PROVIDED you have an invitro capable isolate.




The other plus to using coffee is that it dramatically speeds up mycelium growth.  Even if all you wanna do is transplant the cake to a woodchip bed, coffee will make that white grow!

harrb :mushroom2:


--------------------
Everything I need... leave it to the trees... it can make me feel better!

Extras: Filter Print Post Top
Offlineticktock
Seeker
 User Gallery

Registered: 10/02/04
Posts: 389
Loc: Just ask the rabbit.
Last seen: 14 years, 3 months
Re: The Coffee Project [Re: Hippie3]
    #3408118 - 11/25/04 12:58 PM (19 years, 7 months ago)

I think that the bottom line is that diluted coffee shows promise during colonization, but is bad to add to casings because of increased contamination risk. Molds seem to like it as much as fungus mycelium do.

(What's the right plural for mycelium?)


--------------------
Don't panic!

Extras: Filter Print Post Top
InvisibleGyromitra
Fungsmurf
 User Gallery

Registered: 10/30/04
Posts: 84
Re: The Coffee Project [Re: ticktock]
    #3408572 - 11/25/04 03:39 PM (19 years, 7 months ago)

>>"(What's the right plural for mycelium?)"

Mycelia. In the scientificalish papers it seems like they mainly use that word only when talking about different species/strains; multiple colonies of a single clone seems to be referred to more as one single mycelium. Not an official definition, just how I've seen it used. Kind of like the plural of one species of fish is "fish", but the plural of different species or genetic lines is "fishes". A group of peyote plants is "cacti", but peyote, san pedro, and Pereskiopsis are "cactuses". Blahblahblah.

Anyway I'm posting here to add a little more experience with coffee: 50% coffee soaked WBS grew the same as without coffee at lower temperatures for several species for me. Lower being 65-70?F. I haven't tried it in an incubator yet. I don't incubate everything because mycelia tend to move faster than I do and get overcolonized when I'm not looking- slowing them down a little gives me more time to catch up.

The cofee soaked seed did grow just fine though, and maybe the extra nutrition will add a little to fruiting at colder temperatures. W8nC.

Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | Next >

Shop: Mushroom-Hut Mono Tub Substrate   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* The Hominy Project
( 1 2 3 4 5 6 7 8 9 all )
Hippie3 80,440 173 03/10/04 12:16 PM
by Psychoslut
* Bulk Neglect Report *completed*
( 1 2 3 4 5 all )
mycofile 62,331 92 08/07/02 04:25 AM
by nonsence
* Popcorn as grain substrate?
( 1 2 3 4 ... 26 27 )
Baby_Hitler 163,991 521 08/12/03 09:04 PM
by lalala
* Preparing rye grain - TEST RESULTS
( 1 2 all )
AnnoA 30,780 27 07/17/03 03:43 PM
by Snobrdr311
* Multi Substrate Test - failed
( 1 2 3 4 ... 9 10 all )
AnnoA 84,959 191 02/11/04 07:30 AM
by Hippie3
* Rye
( 1 2 3 4 5 6 7 8 all )
Magash 115,238 149 10/24/04 02:51 AM
by TheGreatButtPaca
* Eatyualives Lazy Mofo Bag Tek
( 1 2 3 4 5 all )
eatyualive 69,374 97 09/09/03 01:28 PM
by BatFly
* Small volume straw pasteurisation tutorial [not realy a growlog].
( 1 2 all )
G a n j a 24,801 32 03/19/04 12:57 AM
by baltazar

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Ythan, Anno, Thor, Link, Seuss
172,811 topic views. 0 members, 0 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.005 seconds on 13 queries.