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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation > Mushroom Cultivation Archive > Substrates
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Re: The Coffee Project [Re: Abermelin]
    #4034187 -

I've got some o/i's...

Not a great experiment, but w/e lol

1st flush was dunked in water, got about 34 wet grams.

2nd flush, well i'm dunking it in 50% regular brewed coffee right now, if i remeber to post back i'll show pics of the coffee dunk, sry, didnt have a camera to show 1st flush...

I'd just like to see if it works with me and french roast :rotfl:

:thumbup:

-Gnostic

btw....that was a Bunch of shit to read :smirk:


--------------------
For the sake of those seeking knowledge.
I've provided some in my signature.
Double Tek Pictorial
Getting the most out of your casings!, A pinning strategy.
Soaking wbs - The Final Answer by Agar
50/50 Coco Coir versus 50/50 Peat Moss.

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Re: The Coffee Project [Re: Abermelin]
    #4034929 -

Quote:
Abermelin said:
Does coffee affect the potentcy of the mushrooms?



If the potency doubled, someone would have noticed by now. Something like a 10% increase would be so tough to measure. You would have to dose two identical brains, and then figure out how to tell what's going on inside.


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Don't panic!

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Re: The Coffee Project [Re: ticktock]
    #4035463 -

ill take that as a no then.

mimosa hostilis root bark (DMT) to increase potentcy.

Coffee to speed up incubation times.

its all starting to come together.

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Re: The Coffee Project [Re: Abermelin]
    #4035844 -

Quote:
Coffee to speed up incubation times.

its all starting to come together.


:rotfl: :thumbup: :wink:

-Gnostic


--------------------
For the sake of those seeking knowledge.
I've provided some in my signature.
Double Tek Pictorial
Getting the most out of your casings!, A pinning strategy.
Soaking wbs - The Final Answer by Agar
50/50 Coco Coir versus 50/50 Peat Moss.

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Re: The Coffee Project [Re: IGnosticAbhorI] * 1
    #4156327 -

I recently did an experiment where I hydrated the casing with solutions of caffeine in varying concentrations: 1.0 * 10-2 to 1.0 * 10-5 M in steps of one magnitude, and also controls. Three fruiting containers of each concentration was used.

1.0 * 10-3 M is equivalent to 100 mg caffeine in 515 ml of solvent (water).

Results:
10-2 M was not good, the pin formation was delayed by 3 days compared to the control, and most mushrooms emerged not from the casing, but poked up straight from the grain at the edge of the container.

10-3 to 10-5 M: fruited on average 2 days earlier than the controls, but this was not statistically significant. Which I would say only suggests more experimenting.

There were no significant differences in yield in any of the concentrations.

The data:
Days from casing application to first pins. The resolution is really one whole day:
10-2 M: 19 +- 1.1
10-3 M: 14.3 +- 0.7
10-4 M: 14 +- 1.1
10-5 M: 14.3 +- 0.7
Control: 16 +- 1.1

Wet weight in grams (100 g of colonized rye per jar):
10-2 M: 23.8 +- 2.4
10-3 M: 23 +- 6.0
10-4 M: 22.7 +- 1.5
10-5 M: 23.1 +- 5.0
Control: 22.4 +- 0.9

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Re: The Coffee Project [Re: Pinback]
    #4156510 -

Excellent, thank you!


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: The Coffee Project [Re: Anno]
    #4156919 -

I second that  :thumbup: :wink:


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Re: The Coffee Project [Re: agar]
    #4157131 -

Interesting data. I'm still of the opinion it's the nutes, not the caffeine. Coffee is perhaps going to shine the brightest on invitro grows. Colonization times seem to be only slightly better than without coffee. What I notice time and time again is the 'time to pin' and eventual pinsets seem much better with coffee than without. This is true so far of all edible, active, and sclerotia producing species(with regards sclerotia production, not fruiting) I've experimented with.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: The Coffee Project [Re: Pinback]
    #4157212 -

Excellent data!!!


Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"

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Re: The Coffee Project [Re: Hippie3]
    #4167726 -

this may seem a bit off topic, but i have been sprinkling used coffee grounds on my outdoor patches for years now.
it never seemed to hurt a thing.
and my grandma said, it keeps the bugs out of her radishes.
it seems to work for mushrooms too. :smile:
nice thread Hip :cool:

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Re: The Coffee Project [Re: Hippie3]
    #4169616 -

the coffee really seems to do the trick. having jars like that after 26 days..it seems to really boost the growth. but somehow i don't think its the caffeine. a way you could find out though, is to grind up a caffeine pill and blend it into your substrate.
growing mushrooms on coffee! i like.


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May all beings with whom we are inseperably interconnected, be fulfilled awakened and free. May there be peace in this world and throught the entire universe, and may we together complete the spiritual journey.

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Re: The Coffee Project [Re: ungskabning]
    #4281608 -

My last batch of rye I put in the coffee grounds from one pot. It seems the most practical thing to do because ou already have them in the coffemaker from the morning. I also have lots of brew houses who will gladly donate used espresso beans and I have already seen that elm oyster will colonize them despite being radicly out of whack with pH. Here is my question is this magic stimulatory stuff in the oils that are leached into the brew or do they also exist in the left behind grounds? Cause growns definetly get eaten.

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Re: The Coffee Project [Re: Pie]
    #4282197 -

Both. It's just good fungi food. That's all. It's not the caffeine because I've used decaf and it works just as well. It's just a source of nutrition for the fungus. I've used it with success on all edibles, medicinals, etc. It works well with straw and dung lovers, and my wood lovers are also hydrated with coffee. All like it. It's no magic bullet, just a suppliment that works well.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: The Coffee Project [Re: RogerRabbit]
    #4288236 -

Hey RogerRabbit, I just started once again and I decided to give the coffee soaked rye a try. I soaked rye grain in 50/50 coffee for 20hrs. Then boiled for a few minutes, strained, loaded, and pc'd for 2hr20min. There are seven gt quarts, and seven b+ quarts. All from syringes inoculated on the 31 of last month. The first three days they were incubated at room temp, due to faulty heaters. Its been 12 days with one jar all white, and the rest trailing shortly behind. These have definitely colonized faster than my last tries with millet/no coffee, but the main reason I tried the coffee was that this is going to be an invitro grow. My incubators are clear tub in tubs. Since this will be an invitro grow should I cut the heat at some point?


--------------------
Let me out of this place
I'm outta place
I'm in outer space
I've just vanished without a trace
I'm going to a pretty place now where the flowers grow
I'll be back in an hour or so

[quote]Abuse said:
the dea can go fuck themselves! with the internet, the impossible is possible![/quote]

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Re: The Coffee Project [Re: luckytriple6]
    #4290252 -

i have a whole brown rice cake going right now (just innoced) so we'll see how it goes. but once fully colonized should i dunk it in the coffee or what ? how would i go about using coffee, should i mix it when i spawn to h/poo or dunk it when fully colonized??

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Re: The Coffee Project [Re: Lelio]
    #4293285 -

I would recommend room temperature for all mycology grows. I do not own an incubator. They are more trouble than they're worth.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: The Coffee Project [Re: RogerRabbit] * 1
    #4294190 -

i have one question...
what kind of mycorrhizae is this?
i can answer your question....it's because cAMP accumulation....
caffeine and its metabolite has "phosphodiesterase inhibitor" function...so accumulated cAMP had played a role....its downstream .... cAMP have various function...especially "stress signalling, starvation signalling....in microbe....in mammalian...caffeine has function..to increase concentration of cG
MP...there are a few of reports on cAMP effect on fungi....agaricus bisporus..etc......
plz....try to find out report...
good luck.....
it'so lucky....i'm also tried to use coffee ground to make mushroom compost.....

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Re: The Coffee Project [Re: RogerRabbit]
    #4294380 -

RogerRabbit said:
I would recommend room temperature for all mycology grows.



Depends what country you are in! my room temp is 16-20C.

I have 2kg of dry wheat which had about 40% dilute coffee added to it. Will one crushed egg shell be enough to buffer the ph in 4kg of wet wheat with coffee?

I am going to grow these invitro in 2 3litre microwavable containers which have filters siliconed to them.

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Re: The Coffee Project [Re: blackout]
    #4296675 -

I doubt it. It will take the egg shell too long to decompose enough to do any good. By then, your project will be finished. Hydrated lime is water soluable, so works immediately. I would recommend a room heater if your house is that cold. It will make you more comfortable as well. Anything above 22C or so will be fine. 24 to 25 is probably perfect in my opinion. Higher than that seems to favor contaminants.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: The Coffee Project [Re: RogerRabbit]
    #4297673 -

ditto here on the coffee grounds. since the coffee dunk has given good result i've added grounds and blood meal to my pf tek. hope it woks, will keep posted on this and my pig feed recipe.

go myc...


--------------------
You are a filipina sex goddess who wants to fuck me until I fall asleep, so then you can tickle my balls and see if the legend of my diamond filled nutsuck is true.  I am a white man from costa rica, who smells like lime jello.


I can flash/jtag/repair 360's, pm for details.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation > Mushroom Cultivation Archive > Substrates
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