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Offlinelsdwanted
<3 mushies

Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 3 years, 3 months
Re: The Coffee Project [Re: Silven]
    #3196582 - 09/29/04 07:14 PM (16 years, 6 months ago)

i read 21 pages, and thats my conclusion as well, and yes i believe you can do that with brf, go through the whole thread, it says it several times towards the middle i believe


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Offlinericelicker
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Re: The Coffee Project [Re: lsdwanted]
    #3197702 - 09/29/04 11:34 PM (16 years, 6 months ago)

It's crazy this has been going on since Jan and there are no significant results......or teks I should say.


--------------------
"my brain waves travel at half the speed afta we..." -del tha funkee

"Two men looked out through prison bars...one saw the mud, one saw the stars." -anonymous


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Invisiblemrnobody998877
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Registered: 09/01/04
Posts: 8
Re: The Coffee Project [Re: RogerRabbit]
    #3199009 - 09/30/04 07:32 AM (16 years, 6 months ago)

Quote:

For example, I've only seen half a dozen or so PE fruits form invitro, but tons of PR, BR, TX and Huatla.




What is the "real" name of this strains ?

PR = Puerto Rico ?
TX = Texas ??
BR = ??


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Offlineduncan8r
Scum

Registered: 08/07/01
Posts: 91
Loc: Syd, Australia
Last seen: 5 years, 6 months
Re: The Coffee Project [Re: Lifenergy]
    #3199041 - 09/30/04 08:11 AM (16 years, 6 months ago)

yeah I did.... stoopidly.

then I read an older post here on the shroomery saying it wouldnt work... popcorn with the same syringe worked a treat, as a direct replacement for BRF in PF style jars, nope.

duncan8r


--------------------
Not all chemicals are bad. Without chemicals such as hydrogen and oxygen, for example, there would be no way to make water, a vital ingredient in beer. --Dave Barry


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 15 years, 27 days
Re: The Coffee Project [Re: Silven]
    #3199078 - 09/30/04 08:53 AM (16 years, 6 months ago)

So, since it seems everyone else has quit.. I have begun my own adventures into the Coffee Project.  This first one is basic.


** CONTROL GROUP **

First 6 jars prepared:

3 1/2 cups vermiculite
  3/8 cups brown rice flour
1 7/8 cups water

Everything was mixed together, added to jars, covered with a dry layer of vermiculite, lids applied, two layers of foil added, and boiled slowly for 1 hour from the beginning of the boil.

Innoculations:

2 jars will be innoculated and labeled equadorian
4 jars will be innoculated and labeled Thai Super Strain


** COFFEE GROUP **

6 more jars prepared:

3 1/2 cups vermiculite
  3/4 cups brown rice flour
1    cup water
  7/8 cup coffee

Again everything was mixed in a clean bowl.  I added the mixed substrate to 6 jars and applied a dry layer of vermiculite to each, put on the lids, covered with two layers of foil and placed in a seperate pan to boil slowly for an hour.

Today when I innoculate:

I will innoculate 2 jars with equadorian and label accordingly and
I will innoculate 4 jars with Thai Super Strain and label

-------------------------------------------------------------------

All of these jars will be incubated together in my incubation chamber that is kept between 80 and 85 degrees.  Humidity is currently at 85% and I am working to get that up.

Next update: 4 days from this post.

- Silven :mushroom2:


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
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Re: The Coffee Project [Re: lsdwanted]
    #3199091 - 09/30/04 09:02 AM (16 years, 6 months ago)

lsdwanted said:
Quote:

i read 21 pages




You must have a smaller resolution or something?  I'm only getting 14 pages still even with our posts added.

Either way, thanks for the info up there :smile:

I'm thinking about undertaking the task of organizing all this into some sort of readable table.  That way maybe we could break up some experiments that need done with this to help us determine what the factor is that was giving such good results to people and bad to others.  I believe that Roger has us on the brink of a great find, and we need to keep pushing forward.  Anyway, lemme know what you think.

- Silven :mushroom2:


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


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OfflineFrappy
Strange

Registered: 09/17/04
Posts: 1,280
Loc: South Florida
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Re: The Coffee Project [Re: Hippie3]
    #3203030 - 10/01/04 06:37 AM (16 years, 6 months ago)

I'm sorry if this has been mentioned, but why not use caffeinated water instead of trying to dissolve caffeine pills in water? This could help distinguish whether it is the coffee of caffeine. Check out Water Joe.

http://www.waterjoe.com/main.html


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OfflineRogerRabbitV
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Re: The Coffee Project [Re: Hippie3]
    #3208462 - 10/03/04 12:02 AM (16 years, 6 months ago)

Unfortunately, this thread has come full circle and is just repeating itself.

SUMMARY: Based on my results.

I don't do brf cakes, so can't testify one way or another. Those who have used coffee with pf style cakes have reported somewhat better results than without coffee, but nothing fantastic other than reduced colonization times.

My experience is that coffee is best used with whole grains like rye or popcorn, where the results are simply fantastic. I did dozens of side by side tests last year before ever publishing any results. The pictures from these tests have been archived on another board.

I've also had good luck mixing a bit of spent coffee grinds in with the peat/verm in casings. Not a lot of coffee grinds, no more than 10%.

I've had excellent luck using weak coffee to hydrate the compost/manure used as bulk substrate. All of the moisture you add to the compost/manure to reach field capacity can be weak coffee. Others have reported increased contamination with this method, but I have not.

I've had terrible luck using coffee in any way with straw. It contaminates.

Using coffee to hydrate grains is especially effective for invitro use.

Using coffee to hydrate grains for sclerotia producing species like mexicana and morel will come close to doubling your yield.

I've noticed no improvement at all by using coffee in agar.

I hope this helps. Good luck guys.


Edited by RogerRabbit (10/03/04 12:07 AM)


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InvisibleEonTan
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Registered: 08/18/04
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Re: The Coffee Project [Re: RogerRabbit]
    #3210998 - 10/03/04 09:36 PM (16 years, 6 months ago)

IME P.mexicana colonized ryegrass seed with plain water faster then ryegrass seed with 1/2 strength coffee water. Sclerotia production at 3 weeks is higher without the coffee with the same clone. Two jars with coffee have yet to finish complete colonization at 21 days.

:confused: Rye grain only?


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OfflineNNY
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Registered: 07/17/04
Posts: 120
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Re: The Coffee Project [Re: Hippie3]
    #3214921 - 10/04/04 07:06 PM (16 years, 6 months ago)

checking back in... the cubes i did had been 100% colonized for a while and have been cased with straw/manuer. the pan cyan jars are only 50% colonized, they are growing so painfully slow. both types were about 40% coffee 60% water


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Offlinemycoguy
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Registered: 03/25/04
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Loc: PNW
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Re: The Coffee Project [Re: Hippie3]
    #3216856 - 10/05/04 02:31 AM (16 years, 6 months ago)

i have had nothing but contams, while experiementing with grains hydrated in coffee. I even had some pc'd jars of corn contam after about a month of sitting in the closet. They hadn't even been innoc'd yet.


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Yahoo! Pacific Northwest Mycology Group


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Offlineamyloid
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Registered: 03/08/03
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Re: The Coffee Project [Re: RogerRabbit]
    #3217463 - 10/05/04 10:42 AM (16 years, 6 months ago)

Just to tack on some more of those good old results to the coffee hype.

this kid added 2 cups of whatever coffee his mom drinks into a pot of boiling popcorn. this was brought to the right moisture level and pc'd. after innoculating with a known strain these jars were closely observed for 2 weeks in comparison to coffeeless popcorn.

the findings are, yes according to this experiment it seems pretty obvious that coffee increases speed of growth in comparison to no coffee. but something happened the kid didnt really expect, not only did the mycellium engulf substrate faster, but when shaken it seemed to increase the frequency of new colonies of mycellium. this may just be the effect of growing faster. but i was thinking more along the lines of how MSGs work, (splitting taste budss to increase flavor). something along the lines of splitting hyphae into more "offshoots"


id still like to take this coffee project to the next level. its very exciting to have found an additive that shows noticable effects. i cant wait until we can guess at why, and what could be used that could even work better.


--------------------
"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Offlineiamgod
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Re: The Coffee Project [Re: amyloid]
    #3234938 - 10/09/04 12:28 AM (16 years, 6 months ago)

I didn't want to start a new thread

heres my experiment. I had two jars of fully colonized rye grain. i Filled one of the qaurt jars with a water/caffeine anhydrous solution and the other with water/folgers solution. these will sit in my fridge for 20 hours and will then be drained and cased seperately. As a control 2 more colonized rye jars are being dunked and cold shocked with a water/h202 solution. They will also be drained and cased. We'll see it if it is the nutes or the caffeine.

Does instant coffee even have nutrients?

I will make a new thread with results when they are in.


--------------------
If what you seek is truth then drugs can not offer truth. Drugs can offer the truth of drugs.......Altered states. Truth is not an altered state of mind. ~ Big Headed Nice Guy Who Loves You And Your Dog

http://www.inlibertyandfreedom.com/Flash/Think_It_Over.swf


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OfflineRogerRabbitV
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Re: The Coffee Project [Re: iamgod]
    #3242215 - 10/11/04 09:54 AM (16 years, 6 months ago)

As I have stated throughout this thread, I doubt the caffeine has anything to do with it. I think it's the nutes.

Mycoguy. Any jar not inoculated after a month is likely to contam. It would take over 24 hours at 15psi to kill EVERYTHING. If you did that, you'd be left with gunk instead of popcorn. A one to two hour PC time gives a good "two week window of opportunity" to get the grain colonized with your mushroom mycelium.

EonTan. I'm sure you know, any jar with or without coffee should be fully colonized well before 21 days. Did you remember to use lime to adjust the ph? How about gypsum? Both are required imo, especially with rye grass seed, as it's so dense. I will relate something about mex a that surprised me: The rye berry jars produced WAY more than the rye grass seed jars. Both were hydrated with coffee. They were showing sclerotia production before full colonization, but were allowed to sit four months before harvest. The rye berry quarts produced a tad over half a quart(by volume) of sclerotia, while the rye grass seed quarts produced a bit more than half that much.


--------------------
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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinekingboomer
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Re: The Coffee Project [Re: RogerRabbit]
    #3257160 - 10/20/04 06:48 PM (16 years, 6 months ago)

:mushroom2:  Maybe it's not the caffeine... It could be an alkaloid???


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take me to heaven..
window in time/
through it I'll fly...

-Black Sabbath


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Offlineharrb
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Re: The Coffee Project [Re: Hippie3]
    #3269864 - 10/24/04 01:59 AM (16 years, 5 months ago)

Has anybody tested the coffee project with Azurescens?  I have some vermiculite jars that are almost 100%, and I wouldn't mind trying some different methods with them.  If it would work with Azures, can someone please tell me the steps I would need to take with the cakes???  :eek:


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Everything I need... leave it to the trees... it can make me feel better!


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Offlinedaimyo
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Re: The Coffee Project [Re: Hippie3]
    #3271195 - 10/24/04 01:29 PM (16 years, 5 months ago)

Just wanted to be reply #420 :smile: 

Also, have 3 pints of popcorn that were simmered in 50/50 coffeee/water.  Procedure..36oz water & 3T folgers coffee grounds were mixed in a pot on stove and heated for about 5 mins stirring occassionaly.  Popcorn was added and simmered for ~40mins.  Emptied from pot to strainer, and drained for 30 mins.  Placed on paper towel and patted dry with another.  Loaded into pints with coffee filters on lids, and covered with foil.  PCd for 45mins, removed, shook, cooled, nocd up with EQ Karo water.  This was done Thursday.  Will check on Monday, and report back.


--------------------
"I have sworn upon the altar of God eternal hostility against every form of tyranny over the mind of man."


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InvisibleEonTan
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Re: The Coffee Project [Re: RogerRabbit]
    #3271626 - 10/24/04 03:59 PM (16 years, 5 months ago)

It is not uncommon for jars of mycelium to take more then 10 days to colonize at temperatures less then optimal.

This is the first mention of lime in a thread that actually thought PH change due to coffee might be part of the reason fro enhanced growth. I never use lime in jars of substrate and had absolutely no problem acheiving yields of 3o grams dry+ per cup of ryegrass seed when temps were optimal and seed was at proper moisture content.

My experience was that on RYE GRASS SEED, coffee perepared according to your suggestions offered NO enhancement of growth, it actually slowed growth in a few jars, or was equal in growth to the controls without coffeee.

Maybe RYE GRAIN coffee combo is the way to go. Did not work with ryegrass seeds.


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Offlineharrb
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Re: The Coffee Project [Re: RogerRabbit]
    #3276466 - 10/25/04 08:18 PM (16 years, 5 months ago)

ROGER,
Do you think coffee could work with Azurescens?  I have a few fully colonized pf cakes.  I was gonna soak a mix of alder chips, vermiculite, and small amount of ash from burned leaves (for nitrogen) into a 50/50 mix of coffee and water for about 24 hours.  Then drain the mix and crumble one of the cakes into it, mixing it up nicely.  All of this would be inside a glass jar (like the pics of your experiments).  Then I would place the jar in the refridgerator for a few weeks.  I heard azures like 50 degrees, and I haven't found out yet, but I'm pretty sure my fridge is about 40-45 degrees.  That way, the azures would have constant cold, moisture (as a substitue for humidity), and wood (since its a wood lover).  Can I get your profession advice on this project? Thanks!

harrb  :mushroom2:


--------------------
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OfflineRogerRabbitV
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Re: The Coffee Project [Re: harrb]
    #3281337 - 10/26/04 10:00 PM (16 years, 5 months ago)

I've not tried to grow azures since I began experimenting with coffee, so I can't say. It seems to be good mycelium food, so my guess it that it would help, but it's only a guess.


--------------------
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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Substrates

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