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OfflineNickster_154371
entheogen enthusiast
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Registered: 01/01/04
Posts: 469
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Re: The Coffee Project [Re: RogerRabbit]
    #2521193 - 04/03/04 09:26 AM (19 years, 11 months ago)

Anybody try using caffeinated bottled water? Ive seen it at gas stations, its called Water Joe. I was thinking I would try to dunk one my casings before its second flush with regular h20, then the other with the caffeinated h20 and see what happens.

Here is the link to the water joe website if anyone is interested http://www.waterjoe.com/aboutjoe.html

Edited by Nickster_154371 (04/03/04 02:48 PM)

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Offlinegetmyshroomon
Trip Sydicate

Registered: 05/30/02
Posts: 75
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Re: The Coffee Project [Re: Nickster_154371]
    #2531045 - 04/06/04 10:03 AM (19 years, 11 months ago)

i just noc'd up some wbs jars and one with liquid myc.


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-Relax and take notes while i tokes on the marijuana smoke-Biggie Smalls

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InvisibleHippie3
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Registered: 11/06/99
Posts: 3,090
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Re: The Coffee Project [Re: Nickster_154371]
    #2534598 - 04/07/04 10:24 AM (19 years, 11 months ago)

why not just get some No-Doze or Vivarin [over-the-counter caffeine pills] crush one up, dissolve in water
and make yer own caffeinated water at about a penny a bottle ?


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InvisibleJoshua
Holoman
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Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
Re: The Coffee Project [Re: Hippie3]
    #2534755 - 04/07/04 11:15 AM (19 years, 11 months ago)

Because the caffeine in the bottled water is much more of a pure source for caffeine than pills which contain fillers and dyes.

If you want to isolate the effects of one specific chemical, you want to avoid any impurities.

I've kept my eye on this thread and haven't seen any side by side comparisons of the use of a substrate with caffeine and one without. I am sure this is an oversight on my part. Do you have any links that I could read up on regarding the use of caffeine in a scientific approach?

Thanks for your time.

Joshua


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OfflineGr0wer
always improving
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Re: The Coffee Project [Re: growin]
    #2536557 - 04/07/04 07:16 PM (19 years, 11 months ago)

Quote:

growin said:
how about other stimulants other then caffine?





yea someone should try using meth :laugh:

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InvisibleATWAR
Connoisseur

Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Re: The Coffee Project [Re: Joshua]
    #2538487 - 04/08/04 08:31 AM (19 years, 11 months ago)

Quote:

Joshua said:

Do you have any links that I could read up on regarding the use of caffeine in a scientific approach?





Someone posted a claim that they read something of this type where different substances were tried againsed the growth of mushrooms. In this claim there was said to be a five fold increase in growth with caffeine. I asked for a link to this information, or at least a reference, but none was given. I myself would like to see a side by side comparison with different concentrations and a side by side with coffee and caffeine pills. I could easily do this but my allergy to caffeine will keep me away from consuming the mushrooms (better safe than in the hospital). Perhaps I will try it on an experimental basis only, but in the end it holds no benefit for me, only risks.

I did do one rye jar inoculated with a liquid culture, but I did not notice one iota of difference (not slower or faster colonization, but that was the extent, no tests were done on pinning time). Perhaps my concentration was off...


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InvisibleHippie3
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Re: The Coffee Project [Re: Joshua]
    #2551037 - 04/12/04 11:26 AM (19 years, 11 months ago)

the only 'science'
refers to sclerotia formation see
web page


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InvisibleJoshua
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Registered: 10/27/98
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Re: The Coffee Project [Re: Hippie3]
    #2551976 - 04/12/04 04:56 PM (19 years, 11 months ago)

So basically this thread is based on hype?

The link you provided says only that caffeine induces sclerotia formation.

Joshua


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Great books for inquiring minds!

"Life After Death is Saprophytic!"

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Offlinenife
I'm Dead
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Registered: 12/26/03
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Re: The Coffee Project [Re: Hippie3]
    #2552207 - 04/12/04 08:04 PM (19 years, 11 months ago)

Man I am excited about this project. I don't really have scientific details to add. I just want to thank everyone that has worked sooo hard on this thread and all the information contained in it. Thanks so much. I will be trying this when I begin again


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InvisibleATWAR
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Registered: 01/26/03
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Re: The Coffee Project [Re: ATWAR]
    #2552447 - 04/12/04 09:45 PM (19 years, 11 months ago)

Found a little something in case anyone is worried about caffeine in their mushies as I am. According to this article, Enoki mushrooms grown on coffee husk did not contain caffeine. Although some mushrooms are known for concentrating toxins and heavy metals, it appears that caffeine may be broken down by the fungi metabolites (at least enoki). Perhaps I will give caffeine a shot, especially if it can accelerate pinning times, there are a few species that could prove useful for.

Abstract:
Solid state cultivation (SSC) was carried out to evaluate the feasibility of using coffee husk and spent-ground as substrates for the production of edible mushroom Flammulina under different conditions of moisture and spawn rate. The strain of F. velutipes LPB 01 was adapted for a coffee husk extract medium. Best results were obtained with 25% spawn rate, though there was not much difference when lower spawn rates (10-20%) were used. Ideal moisture content for mycelial growth was 60% and 55% for coffee husk and spent-ground, respectively. With coffee husk as substrate, first fructification occurred after 25 days of inoculation and the biological efficiency reached about 56% with two flushes after 40 days. With spent-ground as substrate, first fructification occurred 21 days after inoculation and the biological efficiency reached about 78% in 40 days. There was decrease in the caffeine and tannins contents (10.2 and 20.4%, respectively) in coffee husk after 40 days. In coffee spent-ground, the tannin contents decreased by 28% after 40 days. These decrease was attributed to the degradation of caffeine or tannins by the culture because these were not adsorbed in the fungal mycelia. Results showed the feasibility of using coffee husk and coffee spent-ground as substrate without any nutritional supplementation for cultivation of edible fungus in SSC. Spent ground appeared better than coffee husk.

Table 2 shows the contents of caffeine and tannins in the fruit body of Flammulina and coffee residues. The fruit body of Flammulina did not contain caffeine and tannins when grown on coffee husk or spent-ground. The contents of caffeine and tannins were decreased at 10.2 and 20.4%, respectively in the fermented husk, which indicated that the fungal strain was able to degrade it partially. In spent-ground there was no caffeine detected after fermentation. This probably was due to its low initial concentration, which could have been degraded completely, but tannins concentration decreased by 28%. There is no literature report about action of Flammulina on caffeine and tannins.


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To give is to live...


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OfflineFungushead
Carnivore

Registered: 03/04/04
Posts: 243
Loc: Washington State
Last seen: 14 years, 10 months
Re: The Coffee Project [Re: ATWAR]
    #2553354 - 04/13/04 02:53 AM (19 years, 11 months ago)

According to an abstract I read over in mushworld, oysters keep the caffeine from the coffee they were grown in:

"Through the analysis of the fruit body and remains after the fructification, we found that this fungus has not degraded the caffeine but it absorbed and accumulated the caffeine into the fruit body. The content of caffeine in the remains of coffee husk after the growth of mycelia and fructification was reduced up to 60,69%, tannins to 79,17%."

Then again this is a study from Brazil apparently translated roughly from Spanish by a browser so it could be wrong.


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2004: The year telemarketing started to shrivel up like a cold pair of nuts.

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OfflineRogerRabbitV
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Re: The Coffee Project [Re: Fungushead]
    #2553672 - 04/13/04 08:19 AM (19 years, 11 months ago)

I first started these experiments a year ago. At the time, I hadn't read of anyone else using coffee to hydrate their popcorn or rye. I came home after spending 3 days camping, and found the coffee left in the pot with a white mold growing on top. I thought this was awfully fast, so I tried soaking my rye in it, then pc'ing. I noticed right away that jars colonized faster, and pinning was much faster as well. I did several side by side tests, but didn't photograph them. The coffee hydrated grains colonize much faster, and if one is growing invitro, they fruit in the jars much more prolific with coffee. That can be seen from some of my pictures earlier in this thread. I personally doubt it's the caffeine that makes coffee work so well. My guess is it's the nutes.


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleATWAR
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Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Re: The Coffee Project [Re: Fungushead]
    #2553889 - 04/13/04 09:50 AM (19 years, 11 months ago)

Quote:

Fungushead said:
According to an abstract I read over in mushworld, oysters keep the caffeine from the coffee they were grown in:

"Through the analysis of the fruit body and remains after the fructification, we found that this fungus has not degraded the caffeine but it absorbed and accumulated the caffeine into the fruit body. The content of caffeine in the remains of coffee husk after the growth of mycelia and fructification was reduced up to 60,69%, tannins to 79,17%."

Then again this is a study from Brazil apparently translated roughly from Spanish by a browser so it could be wrong.




This is what I have feared. I posted info about Enoki mushrooms not absorbing caffeine, but wondered myself about other species. Some are known for concentrating toxins. I know coffee is being used in lower than drinking strength dilutions, but one should still be careful. It would be interesting to know how much the mushrooms actually absorb or concentrate (% dry mass).

This is most interesting, especially since I do grow Oyster mushrooms, and not Enoki. I think I will try to get my hands on a food testing kit before I ingest any mushrooms grown like this. If any of my experiments warrant future investigation I may test for caffeine content as well as do comparisons. $@%*ing allergies...  :thumbdown:


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Offlineforevrgrounded98
Stranger

Registered: 10/25/03
Posts: 283
Last seen: 17 years, 1 month
Re: The Coffee Project [Re: ATWAR]
    #2557023 - 04/13/04 09:14 PM (19 years, 11 months ago)

What would probably colonize faster in a situation like this, a karo innoculation, or spawning with a chunk of live myc.?


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"If I don't see ya before the end of this one, I'll meet ya in the next one and don't be late" - Jimi Hendrix

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Offlinegrowin
addict
Registered: 03/11/02
Posts: 484
Last seen: 16 years, 7 months
Re: The Coffee Project [Re: Gr0wer]
    #2558613 - 04/14/04 10:04 AM (19 years, 11 months ago)

Quote:

Gr0wer said:
Quote:

growin said:
how about other stimulants other then caffine?





yea someone should try using meth :laugh:




or maybe ephedra?
u guys think it can also help shrooms potencty  ?  :confused:

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Offlinegetmyshroomon
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Registered: 05/30/02
Posts: 75
Loc: 4 hours away from the mid...
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Re: The Coffee Project [Re: forevrgrounded98]
    #2559130 - 04/14/04 12:08 PM (19 years, 11 months ago)

Quote:

forevrgrounded98 said:
What would probably colonize faster in a situation like this, a karo innoculation, or spawning with a chunk of live myc.?





:stoned: live myc. mix like 1/4 of a cake into a jar, and it'll be done quick
you can use less but it wont be done as fast :stoned:


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Offlineforevrgrounded98
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Registered: 10/25/03
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Re: The Coffee Project [Re: getmyshroomon]
    #2559534 - 04/14/04 01:54 PM (19 years, 11 months ago)

awsome. I did 12 jars both ways, guess we'll have to see what happens.


--------------------
"If I don't see ya before the end of this one, I'll meet ya in the next one and don't be late" - Jimi Hendrix

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Offlinegetmyshroomon
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Re: The Coffee Project [Re: forevrgrounded98]
    #2560373 - 04/14/04 04:41 PM (19 years, 11 months ago)

...300+ posts


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-Relax and take notes while i tokes on the marijuana smoke-Biggie Smalls

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OfflineGr0wer
always improving
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Registered: 09/16/03
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Re: The Coffee Project [Re: getmyshroomon]
    #2561316 - 04/14/04 09:25 PM (19 years, 11 months ago)

Yea i tried an experiment but i later discovered my new electric range never got the water to full boil. Im going to re-try the experiment soon.

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OfflineGr0wer
always improving
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Re: The Coffee Project [Re: growin]
    #2561324 - 04/14/04 09:27 PM (19 years, 11 months ago)

ephedra is now illegal as of the 12th, so you cant evenn try that.

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