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OfflineAuroricDistortions
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Re: The Coffee Project [Re: FirstAvailable]
    #2461379 - 03/22/04 09:02 PM (16 years, 11 months ago)

Quote:

FirstAvailable said:
has anyone considered using sky rocket syrup? 100mg caffeine per 1oz.

sky rocket (mocha flavor) ingredients:
cane sugar
water
natural & artificial flavors
caffeine
citric acid
sodium benzoate
potassium sorbate (less than 1% to ensure freshness)




Might the sodium benzoate and potassium sorbate harm or kill mycelium? It's worth a try though.


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OfflineFirstAvailable
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Re: The Coffee Project [Re: AuroricDistortions]
    #2465843 - 03/24/04 12:58 AM (16 years, 11 months ago)

Quote:

AuroricDistortions said:
Might the sodium benzoate and potassium sorbate harm or kill mycelium? It's worth a try though.




I duno, are those substances fungicides?


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OfflineAuroricDistortions
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Re: The Coffee Project [Re: FirstAvailable]
    #2467506 - 03/24/04 01:59 PM (16 years, 11 months ago)

"Sodium benzoate can prevent growth of almost all microorganisms (yeast, bacteria, and fungi). It operates best in an acidic environment, and it works synergically with other food-grade preservatives -- most notably potassium sorbate."
Sodium Benzoate


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InvisibleTremor1127
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Post deleted by Administrator [Re: AuroricDistortions]
    #2467576 - 03/24/04 02:24 PM (16 years, 11 months ago)



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Offlinegetmyshroomon
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Re: The Coffee Project [Re: Tremor1127]
    #2469026 - 03/24/04 10:21 PM (16 years, 11 months ago)

im gonna do liquid myc. into verm/brf but instead of water im going to do 50/50 coffee/water and table spoon of dextrose and the pc for 25 min. and ill tell ya what happens, And if that works im gonna dunk in sterile dex. water.

pece.


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Edited by getmyshroomon (03/24/04 10:39 PM)


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Offlinegetmyshroomon
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Re: The Coffee Project [Re: getmyshroomon]
    #2469437 - 03/25/04 12:01 AM (16 years, 11 months ago)

what if i put in some coffee to make liq myc.? what that be worth trying?


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InvisibleATWAR
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Re: The Coffee Project [Re: spacemouse]
    #2469897 - 03/25/04 02:29 AM (16 years, 11 months ago)

Quote:

spacemouse said:
I had seen on the Mycotopia website, a link to a scientific journal that was studying to effects of certain substances on mushroom mycilia. They stated in their journal that caffeine caused A FIVE FOLD increase in the growth rate of this specific mushroom species mycilia.





Is there a link to this information? It would be interesting to know what other substances were tested. I would be particularly interested in what substances hindered growth. I am curious as to know why this was never noticed by more experienced cultivators, especially commercial operations that may have experimented with the use of spent coffee grounds. This has to have been done, and I imagine some caffiene is still left over in the spent grounds. I would think that if this were true, it would be a guarded secret in the commercial world.

Even Stamets mentions the use of coffe grounds, but nothing of accelerated growth/pinning rates. Is it possible that this may only work for certain species? Is it possible that the concentration needed is very specific? I would think this to be true, each species usually has a semi-specific substrate range that it will grow on (some requiring very specific diets), but each has a fairly specific subtrate that it does best on. This is the kind of thing that needs to be tested if we are to improve the process, but the list of things to try is enormous. Additives and chemicals are an even further dive into complexity. The Brassinosteroids that were mentioned awhile back was very promising, but the price was great. The caffiene or coffee brew idea has sparked my interest (as most new trends do, experimenting is all part of the fun) and I plan to try it myself.

Interesting indeed. A five fold increase? Really...


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OfflineRogerRabbitV
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Re: The Coffee Project [Re: ATWAR]
    #2472438 - 03/25/04 10:28 AM (16 years, 11 months ago)

Quote:

ATWAR said:
Quote:

spacemouse said:
caffeine caused A FIVE FOLD increase in the growth rate of this specific mushroom species mycilia.





My research shows mycelial growth is somewhat faster, but not double, let alone five times. Pinning times are reduced, and pinsets are much better than without coffee. Also, coffee makes it possible, as you can see from pictures early in this thread, to use straight rye or popcorn, and get decent invitro production.


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinecrippledavenger
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Re: The Coffee Project [Re: RogerRabbit]
    #2472895 - 03/25/04 02:11 PM (16 years, 11 months ago)

Just had to throw out all my seven jars of coffee rye, due to bacteria contamination, I was wondering why they were so sluggish. I injected with an old partly used syringe. Bollocks!


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Invisibleniteowl
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Re: The Coffee Project [Re: Tremor1127]
    #2473202 - 03/25/04 04:14 PM (16 years, 11 months ago)

Quote:

Tremor1127 said:
has anyone made coffee cakes and didnt case them? i searched and couldnt find any...




The last set of jars that I did had 10% coffee in the water. They did real good. I plan to do this again with 50/50, h20/coffee.


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Don't be bogged down by your past
Don't be afraid of what lies in your future


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OfflineFastem
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Re: The Coffee Project [Re: niteowl]
    #2475704 - 03/26/04 04:10 AM (16 years, 11 months ago)

Im thinking of doing a few jars with just rye like Roger did and seeing what happens for myself. I read the whole thread, he mentions he had to sterilize the coffee. Would that be accomplished by boiling it or microwaving it? Then just mix 50/50 with water, dunk the rye, let it drain and PC it? Sounds like a plan to me. When I do this Ill make sure to post results and pictures :wink:


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OfflineFastem
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Re: The Coffee Project [Re: Fastem]
    #2475707 - 03/26/04 04:12 AM (16 years, 11 months ago)

I forgot to mention I think Ill do it invitro too just for the hell of it.


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Offlineexan
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Re: The Coffee Project [Re: amyloid]
    #2477448 - 03/26/04 05:13 PM (16 years, 11 months ago)

i think im gonna try a water mixture with cocaine, and see what happens... :tongue:


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Offlinegetmyshroomon
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Re: The Coffee Project [Re: exan]
    #2478748 - 03/27/04 08:28 PM (16 years, 11 months ago)

YEAA-AH NIGGA!
lmao!


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Offlinegetmyshroomon
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Re: The Coffee Project [Re: getmyshroomon]
    #2481631 - 03/29/04 10:25 PM (16 years, 11 months ago)

just made some dex water with 1/4 cup of coffee per litre
ill tell ya if anything happens


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Offlinegrowin
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Re: The Coffee Project [Re: getmyshroomon]
    #2482824 - 03/30/04 07:40 AM (16 years, 11 months ago)

how about other stimulants other then caffine?


a growin original


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Offlinecrippledavenger
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Re: The Coffee Project [Re: growin]
    #2517909 - 04/02/04 05:00 AM (16 years, 11 months ago)

Heeeelllllooooooo is there anybody out there!!! This tread has gone very quiet, how are all your experiments going?


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InvisibleHippie3
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Re: The Coffee Project [Re: crippledavenger]
    #2518112 - 04/02/04 08:47 AM (16 years, 11 months ago)

mine are in limbo,
21 qts of pcorn fully colonized, coffee-hydrated with grounds in the jars,
no contams
at about 24 days along.
so far they appear perfectly normal.
i'll be casing very soon
and will update further then.


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Offlineexan
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Re: The Coffee Project [Re: Hippie3]
    #2518980 - 04/02/04 02:57 PM (16 years, 11 months ago)

my foaf, did a mixture of 50/50 h20 and coffee, 175ml and 1 cup of wbs in 1 pint jars, it's been 3 days and jars are amazingly 70% done.


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OfflineRogerRabbitV
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Re: The Coffee Project [Re: Hippie3]
    #2519848 - 04/02/04 08:22 PM (16 years, 11 months ago)

Just remember, use diluted coffee. The only bad results I've had, or others have had is when they used it full strength, or used dilute coffee and the spent grinds. Use either the coffee (diluted) or the spent grinds from your coffee pot, but not both. Too much coffee actually will slow down growth/pinning.


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Amazon ½ Pint Jars, Brown Rice Flour, Paul Stamets, Rye Grain, Vermiculite, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Substrates

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