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chajim
Stranger

Registered: 05/04/15
Posts: 14
Last seen: 7 years, 6 months
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Psilocibe serbica var. bohemica growing at home
#22768749 - 01/12/16 04:22 AM (8 years, 5 months ago) |
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Hi guys, I have been wondering if you can help me with simple yet hard question. I was clonning P. bohemica I picked up in forrest. After battle with infection I got clear strain with strong grow. I was discussing it here: https://www.shroomery.org/forums/showflat.php/Number/22571474 But after some experimentation I am stucked with substrate question. I tryied cardboard, but having difficulties with infection. Nevermind, I will try again with longer sterilization in press cooker. But what substrate I should use? It is not growing on wooden bedding (it is used for animals to pee on it), it is not growing in coco coire...it is not growing on bird seeds...and I thought it should grow on wooden bedding or coco coire. Can you help me please? Thanks!
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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Re: Psilocibe serbica var. bohemica growing at home [Re: chajim]
#22770028 - 01/12/16 12:42 PM (8 years, 5 months ago) |
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which kind of contamination are you experiencing? if it's bacteria you'd try antibiotic agar. carboard doesn't need to be pressure cooked. https://www.shroomery.org/5276/What-are-common-contaminants-of-the-mushroom-culture https://www.shroomery.org/forums/showflat.php/Number/18430998
did you try pasteurizing your woodchips?
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chajim
Stranger

Registered: 05/04/15
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Last seen: 7 years, 6 months
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Re: Psilocibe serbica var. bohemica growing at home [Re: cc2]
#22773306 - 01/13/16 05:20 AM (8 years, 5 months ago) |
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Hi, mostly I am experiencing green mold.
I am pasteurizing in pressure cooker for at least 1 hour. I know some germ´s seeds are capable to withstand high temperature and it is hard to destroy it with steam and higher pressures. I am using older egg conatiners, therefore I do think it can be infected during storage. I will definitely try cardboard. I did read a lot about it, but I am quite unsure. Do not need to be pressure cooked? So - I just let it soak in hot water? Or how I do sterilize it?
Antibiotic agar is far away from my budget posibilities:) But I am not having lot of contamination on agar.
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cronicr


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 5 months
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: chajim]
#22776479 - 01/13/16 09:36 PM (8 years, 5 months ago) |
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This thread was moved from Advanced Mycology.
Reason: i have sebrica on agar and grain and jars of grain mixed with coir all doing great
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 5 months
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: cronicr]
#22776500 - 01/13/16 09:41 PM (8 years, 5 months ago) |
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go agar to grains to a wood based sub, cardboard is simply a backwards step
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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chajim
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Registered: 05/04/15
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Last seen: 7 years, 6 months
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: cronicr]
#22777203 - 01/14/16 04:12 AM (8 years, 5 months ago) |
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OK, thanks. I tried WBS and it didn´t spawn, therefore I was wondering what kind of substrate to use. Thanks for advice!
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chajim
Stranger

Registered: 05/04/15
Posts: 14
Last seen: 7 years, 6 months
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: chajim]
#22777213 - 01/14/16 04:22 AM (8 years, 5 months ago) |
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One more question, please.
What temperatures are you growing serbica in? It is quite cold during autumn and winter in Czech republic and therefore I do suppose spawning should be made in room temperature (around 20 °C) and fruiting around 10 °C. But I am not sure about this. What is your experience?
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chajim
Stranger

Registered: 05/04/15
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: chajim]
#22802248 - 01/20/16 07:31 AM (8 years, 5 months ago) |
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OK. Nevermind.
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stevo

Registered: 04/11/05
Posts: 5,100
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Re: Psilocibe serbica var. bohemica growing at home (moved) *DELETED* [Re: cronicr]
#22958587 - 02/29/16 01:18 PM (8 years, 3 months ago) |
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Post deleted by stevo
Reason for deletion: .
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: stevo]
#22958605 - 02/29/16 01:25 PM (8 years, 3 months ago) |
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 5 months
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: stevo]
#22959058 - 02/29/16 04:20 PM (8 years, 3 months ago) |
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Quote:
stevo said:
Quote:
cronicr said: <img src='https://www.shroomery.org/forums/images/moved.gif'> This thread was moved from Advanced Mycology.
Reason: i have sebrica on agar and grain and jars of grain mixed with coir all doing great
Got any dated pics of the myc? I am working with this species as well. I hear it's a very slow grower.
i\ll try get some pics...not easy with no pours though but i havent' noticed them to be slow at all
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: cronicr]
#22959319 - 02/29/16 05:54 PM (8 years, 3 months ago) |
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Please please please keep this thread updated. These specimens grow where my ancestors are from. It would mean a lot.
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chajim
Stranger

Registered: 05/04/15
Posts: 14
Last seen: 7 years, 6 months
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: Adden]
#23543178 - 08/15/16 08:21 AM (7 years, 10 months ago) |
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Sorry for long incactivity, I was realy upset when I realized my last batch will not grow anymore. I lost it. Therefore I do have no results at this time and I am looking for another season to pick wild mushrooms and start all over again.
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stevo

Registered: 04/11/05
Posts: 5,100
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Re: Psilocibe serbica var. bohemica growing at home (moved) *DELETED* [Re: chajim]
#23544023 - 08/15/16 01:02 PM (7 years, 10 months ago) |
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Post deleted by stevo
Reason for deletion: .
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chajim
Stranger

Registered: 05/04/15
Posts: 14
Last seen: 7 years, 6 months
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Re: Psilocibe serbica var. bohemica growing at home (moved) [Re: stevo]
#23546052 - 08/16/16 05:49 AM (7 years, 10 months ago) |
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Quote:
stevo said: Cardboard might work better when added to your substrate. So where its not really feeding off this nearly nutritionless stuff that it would hardly grow on otherwise, but feeding on the contaminated substrate, and can use that energy to get on the cardboard away from the molds and have better chance at saving it.
My impression experimenting with this species is.
Grain works okay
Grain mixed with wood works better
Even better is well decayed wood mixed with a lot of grain (even like 50/50).
In the end regardless of colonization the more grain the better. It took off quickly into bagged mulch for me. And when fed more mulch it quickly ate that up growing just as fast as the cyans and shit. After this though, it went back to its normal self as the grain fueled out and it lost that initial vigor that allowed it to eat fresh wood.
The type of wood it really wants to eat I think are rotted looking sticks. It would be a hassle to try to pasteurize this type of wood and worry about loss of beneficial microbes and breaking down carbohydrates and all that. So my plan of action would be to do a lot of sterile jars of clean spawn. With other woodlovers you can treat them as primary decomposers and just throw down green wood even if you want and eventually they will eat everything in sight. My guess is these will take a while. They are a little finicky.
Thanks! I started with wild mushroom, get it on agar for cultivation. I was fighting contamination since then. Ofcourse, it was from forrest. After few tryies I manage sustainable growth on agar, but it grew very fast and I have not enough material to let it grow on. Therefore I used only parts of agar, trying to cultivate this strain more. But I failed on bird seeds, I failed on wooden chips, I failed on cardboard, I failed on grains, but it grew very nicely on paper eggs containers. So I went to it and realized it is colonizing very rapidly. Since then I thought I have found substrate. I tryied to case it - I just took coconut coir and some substrate for whole garden, pasteurized it and tryied to get shroom in this. But I failed miserably. I tryied it once again, but unfortunately wasn´t able to get past eggcontainer phase:-/ then my last batch got contamination and I wasn´t able to secure it.
I will definitely try again when new season starts.
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