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Offlinenewbie0203
Stranger
Registered: 01/25/04
Posts: 5
Last seen: 12 years, 10 months
someone please help me
    #2272863 - 01/25/04 11:08 PM (12 years, 10 months ago)

I am a first time cultivator and I cannot seem to get the pf tek to work. I am sterilizing the jars in a pressure cooker, and i think I am doing it right but when I get to the innoculating step I think I am a little lost. I have attempted to innoculate 6 jars and none of them have come through, none of the jars have even produced any white mold. I am sterilizing the needle before each cake and I am using a glove box. I have had the spores for 3 months now, do they go bad? Am i doing something worn in the sterilizing of the jars? Someone please help me!!!!!!!


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InvisibleHanky
wiffle bat.
Male User Gallery
Registered: 08/30/03
Posts: 56,993
Loc: Great Southern Land.
Re: someone please help me [Re: newbie0203]
    #2272868 - 01/25/04 11:10 PM (12 years, 10 months ago)

are you letting the jars cool for long enough?

what are your incubation temps?


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Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]




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Invisiblesimplemachine
Manfly
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Registered: 09/14/03
Posts: 1,981
Re: someone please help me [Re: newbie0203]
    #2272870 - 01/25/04 11:10 PM (12 years, 10 months ago)

How long did you let the jars cool before innoculating? Oh well hanky got to it first!


SM


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Edited by simplemachine (01/25/04 11:11 PM)


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Offlinenewbie0203
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Registered: 01/25/04
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Last seen: 12 years, 10 months
Re: someone please help me [Re: newbie0203]
    #2272894 - 01/25/04 11:17 PM (12 years, 10 months ago)

i let the jars cool overnight


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Invisiblesimplemachine
Manfly
Male User Gallery

Registered: 09/14/03
Posts: 1,981
Re: someone please help me [Re: newbie0203]
    #2272904 - 01/25/04 11:21 PM (12 years, 10 months ago)

where is the syringe from?


SM


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Anonymous

Re: someone please help me [Re: newbie0203]
    #2272936 - 01/25/04 11:34 PM (12 years, 10 months ago)

I bet its a shitty syringe I can make you one that will get things going quick if you have anything to trade


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Offlinenewbie0203
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Registered: 01/25/04
Posts: 5
Last seen: 12 years, 10 months
syringe [Re: fee]
    #2272954 - 01/25/04 11:40 PM (12 years, 10 months ago)

The syringe is from MSC in Canada.


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OfflineDSD
devil

Registered: 12/01/02
Posts: 1,263
Loc: Bad,Bad person - minus ...
Last seen: 4 years, 8 days
Re: syringe [Re: newbie0203]
    #2273080 - 01/26/04 12:21 AM (12 years, 10 months ago)


who's that ?


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InvisibleTHEBOSS
THE BOSS

Registered: 12/16/03
Posts: 1,252
Re: syringe [Re: DSD]
    #2273113 - 01/26/04 12:29 AM (12 years, 10 months ago)

Do you have your lids on loose? Where did you get the instrucions? Are you following them to a "T"? How many cc are you putting in each jar? Are you letting the needle cool before you inject the spores if not you will kill them? Answer these questions and im sure, myself or someone else will have a good enough idea why your haveing these problems. Whats MSC short for?



THE BOSS


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Mushbox Forum


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Offlinenewbie0203
Stranger
Registered: 01/25/04
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Last seen: 12 years, 10 months
Re: syringe [Re: THEBOSS]
    #2273161 - 01/26/04 12:47 AM (12 years, 10 months ago)

Yea I am following the instructions on this site to the "T". I think I might just have some bad spores. How long should i let the needle cool after i heat it with the lighter?


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OfflineDSD
devil

Registered: 12/01/02
Posts: 1,263
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Re: syringe [Re: newbie0203]
    #2273173 - 01/26/04 12:50 AM (12 years, 10 months ago)


so who is SMC ?


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Anonymous

Re: syringe [Re: newbie0203]
    #2273221 - 01/26/04 01:06 AM (12 years, 10 months ago)

you should flame the lighter till completly red then wrap it in a piece of alcoholesoaked paper towel that cools it and what I do is keep it in the alcohole soaked towel and stab through it into your injection site. if your not cooling the the needle tip you are killing the spores coming through it ya who is SMC can you give us thier web site?


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OfflineMex
Mother Earth
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Registered: 12/25/03
Posts: 256
Loc: ITA ~ Earth ~ 13:20
Last seen: 9 years, 8 months
Re: syringe [Re: newbie0203]
    #2273790 - 01/26/04 06:25 AM (12 years, 10 months ago)

I had the same problems at the beginning... I tried everything,
sterilization method, cooking pressure, etc: the cakes got always hard
to colonize. :frown:
One day I've been putting in the substrate (for fault :crazy:) sooo
much BRF that the jar was almost white.
Know what? That jar was the only one that colonized in 13 days! :cool:

So I would suggest you something: if you think you've been following
all the rule about sterilization & co., put some more BRF (nutriment)
in the substrate. It should maybe work.

Good luck! :thumbup:


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Invisibleutopianglory
Spunkmuffin
Registered: 07/20/02
Posts: 965
Re: syringe [Re: newbie0203]
    #2273812 - 01/26/04 06:38 AM (12 years, 10 months ago)

Get some rubbing alcohol and you will not have to flame the needle and wait until it cools. With rubbing alcohol you can apply, inject, apply inject. Very convenient.

Also, with the fact you have no growth make sure your glasses have not been soaked in any disinfectant for too long, once this happens you will have pretty much no luck in getting growth - ever (with these glasses/jars/whatever).

And finally, verify whether your syringe actually has anything in it. Try looking to see if you see anything floating in it first (no real comfort but atleast you will know whether there is likely to be *anything* that constitutes spores of something in it). I would suggest in future you purchase your spores from reputable vendors that are sponsors of this site. The shroomery does not tolerate inferior vendors (not saying that your vendor is necessarily bad, just that we trust our vendors here - we can't say the same about yours).


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Offlinenewbie0203
Stranger
Registered: 01/25/04
Posts: 5
Last seen: 12 years, 10 months
spores [Re: utopianglory]
    #2274403 - 01/26/04 12:31 PM (12 years, 10 months ago)

MSC stands for mushroom spore company, and i looked at the syringe and there are little black specs floating in the water which i assume they are spores. I think that you are right in that i need to get better spores can you tell me a website or a way to order good spores, maybe there is an easier tek for me to try? Any info you can give me about a better way to grow or a spore site that is good would be great... thanks to everyone that responded i appreciate the help!!!


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OfflineMex
Mother Earth
Male

Registered: 12/25/03
Posts: 256
Loc: ITA ~ Earth ~ 13:20
Last seen: 9 years, 8 months
Re: spores [Re: newbie0203]
    #2274472 - 01/26/04 01:06 PM (12 years, 10 months ago)

Regarding the spore site just take a look to the sponsors of
shroomery.org , like Ralphster etc...
The little black specs floating on the syringe yes, are spores. :smile:


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Invisiblesimplemachine
Manfly
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Registered: 09/14/03
Posts: 1,981
Re: spores [Re: newbie0203]
    #2274637 - 01/26/04 02:27 PM (12 years, 10 months ago)

Well you can't get much easier than the pf tek...maybe if you could describe EXACTLY and in DETAIL what you did, maybe we can figure out where you went wrong...


SM


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Offlinedannynosleeves
newbie
Registered: 12/30/03
Posts: 37
Last seen: 12 years, 8 months
Re: spores [Re: newbie0203]
    #2274660 - 01/26/04 02:38 PM (12 years, 10 months ago)

SMC sucks. I ordered a cd in the beging of decemeber and have yet to see it and I email them daily with no reply.


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OfflineKreeprCeepr
Dont mistakelack of talentfor genius!

Registered: 01/09/04
Posts: 359
Loc: Oregon
Last seen: 10 years, 8 months
Re: spores [Re: dannynosleeves]
    #2274742 - 01/26/04 03:13 PM (12 years, 10 months ago)

I am in the same boat as newbie, i oredered spores from ExoticAlternatives.com because i am short on cash and i got 3 syringes for 19.50 and free shipping. thinking it was a good deal. all of my syringes were completely clear, i got PR's, B+, and GT's. all of my GT's have gotten Green mold, most of my B+'s have gotten cob web, and my PR's are the only ones that have shown mycilium. (its the coolest feeling to see your first mycilium!!!) I dont think the contams were my fault, i sterilized for 90 minutes, let cool overnight, and then went to my piercing studio (extremely clean) to innoculate, i used a flame to sterilize my needle between each jar. So im giving Ralph a try (plus he is only a state away1!) next time i get some spores.When the syringes arrived at my house, i opened the package and there was water leaked from the syringes in the bag...pretty disapointing for your first time grow. I'm using 2 parts Verm, 1 part water, 1/2 part BRF, 1/2 Part Millet flour, could this also be a problem? Thankyou for your advice it is appericiated very much, good luck to you newbie, we should have been smart and went to a shroomery sponser. Theres always next time!


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"My lands are where my dead lie buried" Crazy Horse R.I.P

Native Pride


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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: spores [Re: dannynosleeves]
    #2274755 - 01/26/04 03:20 PM (12 years, 10 months ago)

I ordered a book from MSC 2 months ago and still waiting for the fucking book. His name is Jack, he is from Canada and sucks very much.


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Sr_Setahongo


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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