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OfflineSloppyJoseph
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Re: Pastywhyte's Easy Agar Tek [Re: NDStepp84]
    #22718051 - 12/30/15 06:57 PM (8 years, 1 month ago)

When condensation creates pools of water post-inoculation what should happen though? Should you tip the plates on their sides to keep the agar from drowning? This happened in a few of my last plates.


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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: SloppyJoseph]
    #22718069 - 12/30/15 07:02 PM (8 years, 1 month ago)

I work with all my pasty plates upside-down, so I guess it gets dumped out...


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Invisibleredrum
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #22718086 - 12/30/15 07:05 PM (8 years, 1 month ago)

Thanks for the response guys.

How do you work with them upside down? Can't I just open the cover and dump out any water that's accumulated?


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OfflineNDStepp84
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Re: Pastywhyte's Easy Agar Tek [Re: redrum]
    #22718102 - 12/30/15 07:08 PM (8 years, 1 month ago)

Yes, you can dump it on the floor of the SAB :thumbup:


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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: redrum]
    #22718158 - 12/30/15 07:21 PM (8 years, 1 month ago)

Quote:

redrum said:
Thanks for the response guys.

How do you work with them upside down? Can't I just open the cover and dump out any water that's accumulated?




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OfflineSloppyJoseph
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #22718199 - 12/30/15 07:29 PM (8 years, 1 month ago)

Quote:

Munchauzen said:
I work with all my pasty plates upside-down, so I guess it gets dumped out...



:borat:


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InvisibleMudaFuka
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Re: Pastywhyte's Easy Agar Tek [Re: SloppyJoseph]
    #22718252 - 12/30/15 07:39 PM (8 years, 1 month ago)

All my plates sit in a tote at room temperature for between 4 days and 3 months befor I inoculate them. By the time I get to them there is no condensation. If there us any pooling I dump it in my SAB. Unless I'm trying to germinate spores. I like a bit of pooling fir spores germination.


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Offlinetripdawg420
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
    #22718258 - 12/30/15 07:41 PM (8 years, 1 month ago)

3 months wow i make them as needed lol thats hella long they dont dry up :shrug:


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InvisibleMad Season
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Re: Pastywhyte's Easy Agar Tek [Re: tripdawg420]
    #22718272 - 12/30/15 07:44 PM (8 years, 1 month ago)

I know I can't be the only one who doesn't care there's water on my agar :/. Why is this such a big problem for people? It hasn't hindered anything for me. Just wondering lol.


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InvisibleViolet
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Re: Pastywhyte's Easy Agar Tek [Re: Mad Season]
    #22718308 - 12/30/15 07:51 PM (8 years, 1 month ago)

Same here.  I'd rather not have it, obviously, but it has never done me any real disservice.  I do pour or shake it out to minimize it (I might only suggest pouring, not shaking, in a still-air box) but that's just because I can.  Everyone can at least pour out a lot of it.  Or, after getting your transfer wedge stuck in there, keep it upside-down.  You can also try to shake off as much condensation as you can before it finishes cooling so that it's a part of the liquid in the bottom when it gels, that helps a lot too.

Between those two things combined I have never had a problem.  It can only be a real problem if you're dealing with bacteria.
I don't have my thumb on it, but since the condensation I get varies, there's a way to reduce it, something to do with the dynamics that cause condensation to begin with.  Hate to sound like a simpleton by leaving it at that but that's all I feel like digging into it at the moment.


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InvisibleMajorDick
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Re: Pastywhyte's Easy Agar Tek [Re: Violet]
    #22718351 - 12/30/15 08:04 PM (8 years, 1 month ago)

Had anyone ever tried slowly bringing the temp down after the cycle? Or (possibly same effect) slightly warming up the pc an hour or two after the heat is turned off? Like, not enough to liquify the media.


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OfflinecronicrFacebook
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Re: Pastywhyte's Easy Agar Tek [Re: MajorDick]
    #22718354 - 12/30/15 08:05 PM (8 years, 1 month ago)

yes it can help  but condensation is over rated anyway:lol:


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OfflinePreparationH
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Re: Pastywhyte's Easy Agar Tek [Re: Violet]
    #22718357 - 12/30/15 08:06 PM (8 years, 1 month ago)

When I am done pressure cooking the plates I just pulled the pressure cooker rocker off and take them out and place them on the table.  The condensation goes away slowly.  Is it a bad idea to just pull the rocker off and have boiling steam shoot out/pressure drop so fast?


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InvisibleViolet
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Re: Pastywhyte's Easy Agar Tek [Re: PreparationH]
    #22718366 - 12/30/15 08:08 PM (8 years, 1 month ago)

Quote:

PreparationH said:
Is it a bad idea to just pull the rocker off and have boiling steam shoot out/pressure drop so fast?



Yes, the sudden release of the pressure holding the agar's hyper-boil back will cause an incredibly rapid boil.  The pressure raises the boiling temperature - if you drop the pressure, the temp in the agar stays the same, and makes it boil hard - and nothing boils more than agar except agar with a ton of starch.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflinePreparationH
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Re: Pastywhyte's Easy Agar Tek [Re: Violet]
    #22718405 - 12/30/15 08:16 PM (8 years, 1 month ago)

What should I be doing, let it be for a couple hours? I was yanking them out because when I do them some have tipped over and I end up with agar wedged in the corner of the plate and not flat on the bottom


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InvisibleViolet
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Re: Pastywhyte's Easy Agar Tek [Re: PreparationH]
    #22718427 - 12/30/15 08:21 PM (8 years, 1 month ago)

You don't have to wait until the agar has cooled, just until the pressure reaches atmospheric pressure.  This is merely below boiling temperature.  This is a cinch if your cooker has a meter.  Otherwise it's likely to be down, or close, when you don't hear hissing from the rocker.  If you budge the rocker and it hisses, there's still pressure.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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InvisibleMudaFuka
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Re: Pastywhyte's Easy Agar Tek [Re: PreparationH]
    #22718451 - 12/30/15 08:26 PM (8 years, 1 month ago)

Quote:

tripdawg420 said:
3 months wow i make them as needed lol thats hella long they dont dry up :shrug:



I like having lots of plates on hand. I try to make at least 20 plates a week, weather I need them or not. Sometimes I get too busy for myco work and they end up sitting for a while. I've never had one dry up. I've kept colonised plates around for well over a year befor they even started to look dry.

Quote:

PreparationH said:
What should I be doing, let it be for a couple hours? I was yanking them out because when I do them some have tipped over and I end up with agar wedged in the corner of the plate and not flat on the bottom



Take them out shirtly after the pressure drops to 0. They will stay hot and liquid fir a while.


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OfflinePreparationH
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
    #22718858 - 12/30/15 09:59 PM (8 years, 1 month ago)

Well then......... Glad I know now after 5+ years of using the thing :lol:


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: PreparationH] * 1
    #22721528 - 12/31/15 03:07 PM (8 years, 1 month ago)

They are coming along! :laugh:



I think they've been slow to colonize because its tissue from a mature fruit and because I've had them in a 60-70 degree area. I moved them to a 70-80 degree area.

Quote:

MudaFuka said:
Quote:

CapMeh said:
Hey guys! So I inoculated my plates with clone tissue about 5 days ago, and I'm seeing some action finally! Is 4-5 days a normal wait time to see clone tissue start to run? 7/8 plates show growth :laugh: No signs of contams yet.

here are 2/8 plates


This third plate looks a little different than the others; My first thought was bacteria (maybe my knife was dirty for this plate or something), but then I thought maybe it was just water that had gathered around the tissue, water that might have had a little malt suspended in it and the myc is colonizing it like a mini LC? Let me know what you think



Thanks yall :awehigh:



I can't really see too well but that looks like bacteria to me. Normally my clones run much faster than that. There are variables to consider though. How much nutrition is in your agar mix? What temperature are your plates colonising at? Genetics is a big one too. Some cultures are a lot faster than others. Also if you clone from a mature fruit it will move slower than a clone from a pin.




Regarding the plate that looked like it had bacteria in it, I cant see it anymore o.0 Is it possible the myc has grown over it? Like the new photos above, They are all either fuzzy (tomentose I guess) or some are rhizomorphic already.


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Invisiblepablokabute
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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22723571 - 01/01/16 04:38 AM (8 years, 1 month ago)

Hi guys. 

Do you think it's possible to make use of pastyplates for 'micropogation'?

Well, if it can, it might be a revolutionary way to micropogate using these plates maaaan...


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