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Offlinehomerj
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agar someday [Re: Pastywhyte]
    #22666740 - 12/17/15 08:34 AM (8 years, 2 months ago)

hmm never have tried agar, maybe one day.


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InvisibleInocuole
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Pastywhyte's Easy Agar Tek [Re: homerj]
    #22666744 - 12/17/15 08:35 AM (8 years, 2 months ago)

Did that really require you to change the thread subject?


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Re: agar someday [Re: homerj]
    #22666807 - 12/17/15 09:08 AM (8 years, 2 months ago)

:puppet:


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InvisibleInocuole
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22666811 - 12/17/15 09:10 AM (8 years, 2 months ago)

God damn you BD.


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22667701 - 12/17/15 01:15 PM (8 years, 2 months ago)

I have a question about the plastic (ziploc 16oz) twist top agar plates. After the PC cools, when transporting them from the PC to the SAB, do I seal the lids completely? Like, open the PC, seal them all in the PC, then transport? Do I leave them unsealed after inoculation? If so do I put some freezer bags in the SAB to throw the dishes into after inoc? Is this common knowledge that I'm just not stumbling upon? If there is a link that would answer this, that would be awesome. If not, any help would be much appreciated :heart::shrug:


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InvisibleInocuole
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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22667709 - 12/17/15 01:19 PM (8 years, 2 months ago)

Sounds like you might be sliiightly overthinking it, which is why you aren't finding the info.  For me it's:
PC -> Kitchen counter -> Shelf -> SAB -> Shelf+time -> SAB

No bags unless I'm putting them in the fridge for a long period of time.  You can take more or less precautions, but most of what can happen to the outside of a pasty plate is nothing that wiping with iso before handling won't fix.


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Invisibleshroominmyroom
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22667735 - 12/17/15 01:31 PM (8 years, 2 months ago)

:flyhigh:


Edited by shroominmyroom (12/12/17 04:14 PM)


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22667745 - 12/17/15 01:34 PM (8 years, 2 months ago)

Hmmm perhaps you're right. Either way, thanks for the reply and the experience :] I feel like maybe I should have just gone with the mini rounds so that they are sealed at all times excluding the inoc. Guess I could always mod the twist top lids. Or maybe I'll just sack up and relax a little :facepalm:

Thank you for the tips Shroominmyroom :mushroom2:


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Re: Pastywhyte's Easy Agar Tek [Re: shroominmyroom]
    #22667748 - 12/17/15 01:35 PM (8 years, 2 months ago)

Wait, what do we mean by "sealing" here?  These things should be wrapped in foil around the lids.  What are we sealing that isn't already sealed enough?

Oh I see you have the twist top lids, gotcha, yeah you want to tighten them up when they're not in the PC.  Sorry, I missed that. :facepalm:


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Coir-ca-cola
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Invisibleshroominmyroom
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22667755 - 12/17/15 01:37 PM (8 years, 2 months ago)

:flyhigh:


Edited by shroominmyroom (12/12/17 04:14 PM)


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Re: Pastywhyte's Easy Agar Tek [Re: shroominmyroom]
    #22667760 - 12/17/15 01:38 PM (8 years, 2 months ago)

Let's just call that a symptom of having smoked enough weed.

I thought dude was talking about sealing them in some sterile container, or the PC, to bring back to the SAB and shit. :lol:


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Coir-ca-cola
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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22667763 - 12/17/15 01:39 PM (8 years, 2 months ago)

Scared the shit out of me for a sec there :rofl2:

NOT THE PLATES! NOT MY GUM DROP PLATES!



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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22667768 - 12/17/15 01:41 PM (8 years, 2 months ago)

Actually, here are some pics of them before the PC yesterday


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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22667773 - 12/17/15 01:43 PM (8 years, 2 months ago)

666th post is some fine looking agar.


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22667831 - 12/17/15 02:00 PM (8 years, 2 months ago)

Lets hope that's good luck! :evil: This is my first ever agar attempt after all. I'll make a thread with updates n shit. Does anyone's agar get frothy/foamy on the surface after the cook? I mixed 10g DME and 10g agar with 500ml tap water, cooked it in a pan till it started to simmer/low boil. constant stirring. I poured a couple plates but realized the agar I was pouring had some foam-like accumulations on the surface so I did my best to scoop it out. Is this a normal thing to run into? Any way to prevent it?


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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22667856 - 12/17/15 02:08 PM (8 years, 2 months ago)

I give it a gentle swirl while it's still kinda hot and that helps as much as it can before you start lining the walls with agar but, the typical solution is to raise and lower the pressure more slowly.


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22668111 - 12/17/15 03:26 PM (8 years, 2 months ago)

Ah okay thank you fish :smile:

Okay so this is my current situation.


Got everything wiped down and letting the air settle. Noticed one or two of the plates had sealed and created a vacuum. Not sure if that's going to effect anything. My main concern is the water in the dishes. There's a little condensation along the walls, but there's a drop or two of water rolling around on the top of the agar in a couple of the dishes. Should I try and pour it out into the SAB or leave it there? Thanks in advance!


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Edited by CapMeh (12/17/15 03:34 PM)


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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22668140 - 12/17/15 03:32 PM (8 years, 2 months ago)

Remove the gloves :thumbup:


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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #22668161 - 12/17/15 03:38 PM (8 years, 2 months ago)

Pour it out onto the floor of the SAB.  The vacuum shouldn't matter much as long as you break it gently in the SAB, keep the drafts minimal.


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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22668192 - 12/17/15 03:45 PM (8 years, 2 months ago)

And don't use your lighter inside the SAB. You're asking to ignite fumes and go to the ER.


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