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 Yarry
Old Timer
Registered: 01/05/04
Posts: 23,762
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 Agar Preperation 
#2263387 - 01/22/04 07:05 PM (17 years, 10 months ago) |
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I have got my hands on some nutritive yeast, malt extract and agar. My question is all the formulas list ingredients per gram.. i dont have a gram scale, so does anyone know what it works out into teaspoons or whatever? a measurement for all ingredients in mL would be greatly appreciated! Thanks guys 
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Grumpy Old Man.
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 llamaboy
the weasel thatsnagged the bee
Registered: 11/08/03
Posts: 563
Loc: Portland PNW
Last seen: 12 years, 1 month
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Re: Agar Preperation [Re: Yarry]
#2263412 - 01/22/04 07:14 PM (17 years, 10 months ago) |
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heh usually from what i've heard...a teaspoon is about 3grams...but youknow density matters and all that. *shrug* you can buy a cheap gram scale that is somewhat accurate for around 20$ at your local headshop.
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Yarry
Old Timer
Registered: 01/05/04
Posts: 23,762
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Re: Agar Preperation [Re: llamaboy]
#2263465 - 01/22/04 07:31 PM (17 years, 10 months ago) |
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alas i dont have any money til next friday.. i was jus hopin thered be an easier way, does anyone else have any feedback?
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Grumpy Old Man.
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Anno
Experimenter
Registered: 06/18/99
Posts: 24,145
Loc: my room
Last seen: 1 day, 13 minutes
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Re: Agar Preperation [Re: Yarry]
#2263522 - 01/22/04 07:59 PM (17 years, 10 months ago) |
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>a measurement for all ingredients in mL would be greatly appreciated
I went and measured some agar powder from sporeworks:
10 ml powder(loose) is 5.2 grams.
I don?t have any malt extract here.
Yeast is used like a pinch for 100 ml water.
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Yarry
Old Timer
Registered: 01/05/04
Posts: 23,762
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Re: Agar Preperation [Re: Anno]
#2263541 - 01/22/04 08:06 PM (17 years, 10 months ago) |
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Thanks anno your awesome! anyone have any malt and a scale?
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Grumpy Old Man.
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Anonymous
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Re: Agar Preperation [Re: Yarry]
#2265971 - 01/23/04 04:16 PM (17 years, 10 months ago) |
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I just measured out and weighed my malt extract powder three different times. Scooped up 1 teaspoon full, shook level (or used back of knife to level), and the average of those three was 1 teaspoon = approx. 3 grams...
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Yarry
Old Timer
Registered: 01/05/04
Posts: 23,762
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Excellent, ive got it done now, and its set. My next question is how long after i make up the agar do i have to wait to put my spores in??
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Grumpy Old Man.
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Anonymous
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Re: Agar Preperation [Re: Yarry]
#2266325 - 01/23/04 05:55 PM (17 years, 10 months ago) |
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Just wait for it to solidify and reach room temps...
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Yarry
Old Timer
Registered: 01/05/04
Posts: 23,762
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probably a dumb question, whats the best way to innoculate mycelium? should i just scrape some spores onto it? should i squirt a little spore water on? any advice is appreciated
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Grumpy Old Man.
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YidakiMan
Stranger
Registered: 09/29/02
Posts: 2,023
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Re: Agar Preperation [Re: Yarry]
#2266594 - 01/23/04 07:27 PM (17 years, 10 months ago) |
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You can streak them or use spore water. Streaking will result in a conglomerated mess of substrains. The more substrains you use to inoculate, the better your chance of a successful fruiting. If you are inoculating grain without isolating, this is probably the way to go.
If you wanted to isolate substrains before you fruit, use diluted spore water. You can dilute it enough that you can get as few as 5 substrains out of a few drops. Spore dilutions are better for agar isolation.
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