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OfflineSivarted
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Re: Post Your Cultivation Picture of The Day [Re: Sivarted]
    #22610368 - 12/03/15 07:55 PM (8 years, 5 months ago)

Not shown above: shiitake and brf cakes that I hid away too, cause they weren't actually growing anything identifiable as legal yet.  :smile:

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OfflineSalviaGod
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Re: Post Your Cultivation Picture of The Day [Re: Sivarted]
    #22610388 - 12/03/15 08:02 PM (8 years, 5 months ago)

If i put a small clump of oyster mycelium on agar and it has started expanding on the dish, but also started pinning on the clump of mycelium should i transfer from the new growth on the agar, or clone the pin?

I guess i can do both but i wonder which will be better.

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InvisibleInocuole
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Re: Post Your Cultivation Picture of The Day [Re: SalviaGod]
    #22610414 - 12/03/15 08:07 PM (8 years, 5 months ago)

It's impossible to tell which would be better until you've done both and tested the results.  Maybe the clone isn't really that great, maybe the MS culture is a little more vigorous, but maybe it's the other way around.  Only one way to know.  That's ideally what you should do.  Now, you don't have to clone every pin that pops up on agar.  You'll have an MS culture, for instance, that you like working with, but you know that it pins a lot for whatever reason.  I clone pins that look exceptionally healthy in the hopes that they'll appreciate a real substrate with proper conditions.  Most of them, I either pick and dry before I toss the agar, or I just let them rot there and eventually toss it all.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: Sivarted]
    #22610479 - 12/03/15 08:23 PM (8 years, 5 months ago)

I just got a whole bunch of these myself and Ive been going 1 quart to 3 quarts with them

I really like them so far but I haven't fruited them yet. Was either gunna do little dub tubs or stick them in monos.. what do you do with them?


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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: Sivarted]
    #22610490 - 12/03/15 08:25 PM (8 years, 5 months ago)

Quote:

Sivarted said:
Quote:

mushpunx said:



Hmm. These PE plates are getting close, but still no isolate




What about that makes you believe it's *not* an isolate?  In that process myself with APE, and I'm not very good at distinguishing separate sectors yet, but with one possible tiny exception (that I'm not even really sure I see), that looks like a monoculture to me.





Well it could be actually, I have to let it grow out a little more. I have 20 of these and a few of them looked just like this but tricked me, it had a sector or to show up when it grew out a bit more


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Invisibleazur
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #22610510 - 12/03/15 08:29 PM (8 years, 5 months ago)

Quote:

mushpunx said:
Quote:

Sivarted said:
Quote:

mushpunx said:



Hmm. These PE plates are getting close, but still no isolate




What about that makes you believe it's *not* an isolate?  In that process myself with APE, and I'm not very good at distinguishing separate sectors yet, but with one possible tiny exception (that I'm not even really sure I see), that looks like a monoculture to me.





Well it could be actually, I have to let it grow out a little more. I have 20 of these and a few of them looked just like this but tricked me, it had a sector or to show up when it grew out a bit more



you'll have more show up on this one


--------------------


A cube is NOT a cube.

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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: Pastywhyte]
    #22610519 - 12/03/15 08:32 PM (8 years, 5 months ago)





Quote:

Pastywhyte said:
Quote:

mushpunx said:



Hmm. These PE plates are getting close, but still no isolate




That might be the ticket. You should change media tho, culture looks like it's getting lazy.





Do you think thats what's up? This culture started out ropey as fuck, that pic I just put up was an early plate. I just figured I grabbed from a fluffy sector a while back.
The 20 other isolates Im working on are looking like that too.

So you think I should switch from MEA to like PDA?

I might actually go back to some of the plates from a few sets of transfers back when they just started getting fluffy and grab from a couple other spots :shrug:


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Edited by mushpunx (12/03/15 08:34 PM)

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OfflineSivarted
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #22610569 - 12/03/15 08:43 PM (8 years, 5 months ago)

Quote:

mushpunx said:
Quote:

Sivarted said:
Quote:

mushpunx said:



Hmm. These PE plates are getting close, but still no isolate




What about that makes you believe it's *not* an isolate?  In that process myself with APE, and I'm not very good at distinguishing separate sectors yet, but with one possible tiny exception (that I'm not even really sure I see), that looks like a monoculture to me.





Well it could be actually, I have to let it grow out a little more. I have 20 of these and a few of them looked just like this but tricked me, it had a sector or to show up when it grew out a bit more




Hmm.  So I've been using 4-section plates, but there's obviously not a lot of room for them to grow out, and I have to take transfers pretty early on, or they get to the edges and start overlapping.  Since I'm trying to get to APE isolates to test, would it be worth grabbing some unsectioned ones?

I was already planning on taking them to individual plates eventually, to avoid the mixing and overlap problem with the sectioned ones (the myc is perfectly happy to crawl over the wall and say hi to its neighbors), but I was kinda hoping to delay that as long as possible.

The number of plates is already starting to get scary even with the 4-sections, I can't imagine how nuts it would get if I used single plates.  Lol

Of course, I guess I also have to be prepared to test grow a couple dozen (or more?) wedges, so maybe it isn't the petris I should be worried about.  :smile:

Edited by Sivarted (12/03/15 08:46 PM)

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InvisiblePastywhyteMDiscord
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #22610623 - 12/03/15 08:56 PM (8 years, 5 months ago)

Quote:

mushpunx said:




Quote:

Pastywhyte said:
Quote:

mushpunx said:



Hmm. These PE plates are getting close, but still no isolate




That might be the ticket. You should change media tho, culture looks like it's getting lazy.





Do you think thats what's up? This culture started out ropey as fuck, that pic I just put up was an early plate. I just figured I grabbed from a fluffy sector a while back.
The 20 other isolates Im working on are looking like that too.

So you think I should switch from MEA to like PDA?

I might actually go back to some of the plates from a few sets of transfers back when they just started getting fluffy and grab from a couple other spots :shrug:




I would. I try to change media every 2 to 3 transfers. I bet you get better defined growth and it will be easier to see whats going on. Tho I don't see sectoring in that plate myself.

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InvisiblePastywhyteMDiscord
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Re: Post Your Cultivation Picture of The Day [Re: Pastywhyte]
    #22610658 - 12/03/15 09:04 PM (8 years, 5 months ago)








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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: Sivarted]
    #22610712 - 12/03/15 09:17 PM (8 years, 5 months ago)

Sometimes I have to let the cultures grow out a little bit and let the sectors have a chance to fan out a little. But Im not really all that experienced yet

You will burn through plates though man! I took growth from 20 sectors so every set of transfers goes thru a sleeve .. I use pasty plates to stretch them out

I love the sectioned plates but yea you are limited space wise.


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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: Pastywhyte]
    #22610720 - 12/03/15 09:19 PM (8 years, 5 months ago)

Quote:

Pastywhyte said:
Quote:

mushpunx said:




Quote:

Pastywhyte said:
Quote:

mushpunx said:



Hmm. These PE plates are getting close, but still no isolate




That might be the ticket. You should change media tho, culture looks like it's getting lazy.





Do you think thats what's up? This culture started out ropey as fuck, that pic I just put up was an early plate. I just figured I grabbed from a fluffy sector a while back.
The 20 other isolates Im working on are looking like that too.

So you think I should switch from MEA to like PDA?

I might actually go back to some of the plates from a few sets of transfers back when they just started getting fluffy and grab from a couple other spots :shrug:




I would. I try to change media every 2 to 3 transfers. I bet you get better defined growth and it will be easier to see whats going on. Tho I don't see sectoring in that plate myself.




Oh wow dude every 2-3? Yea maybe Ill start switching back and forth between MEA and PDA each sleeve of plates

How about nutrients? These plates the nutes are a little lower ; maybe 6 grams LME to 10 g agar

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OfflineSivarted
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #22610766 - 12/03/15 09:28 PM (8 years, 5 months ago)

Quote:

mushpunx said:
Sometimes I have to let the cultures grow out a little bit and let the sectors have a chance to fan out a little. But Im not really all that experienced yet

You will burn through plates though man! I took growth from 20 sectors so every set of transfers goes thru a sleeve .. I use pasty plates to stretch them out

I love the sectioned plates but yea you are limited space wise.




How many generations back do you keep?  So far I've kept everything, but as I get further along I'm thinking it might not make sense to keep them too far back, because if the goal is to get isolates to test, there might not be reason to keep a plate that I've already taken transfers from.

Of course, you're talking about going back to previous generations, I'm just wondering if, rather than going through hundreds of disposable petris, it might be worth putting together a few rotating sets of pastyplates.  :smile:

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InvisiblePastywhyteMDiscord
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #22610777 - 12/03/15 09:31 PM (8 years, 5 months ago)

Bring the nutes back up to 2% :thumbup: IMO when using low nutes to stimulate a culture its better to fluctuate between low and normal rather just low.

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OfflineSivarted
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Re: Post Your Cultivation Picture of The Day [Re: Sivarted]
    #22610785 - 12/03/15 09:33 PM (8 years, 5 months ago)

I'm quickly discovering that I'm not just limited by plate space with the sectioned ones, but time as well.  There's only a few days between "spread out enough to see what's going on" and "hey neighbor, wanna fuck?"  :laugh:

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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: Sivarted]
    #22610794 - 12/03/15 09:35 PM (8 years, 5 months ago)

Quote:

Sivarted said:
Quote:

mushpunx said:
Sometimes I have to let the cultures grow out a little bit and let the sectors have a chance to fan out a little. But Im not really all that experienced yet

You will burn through plates though man! I took growth from 20 sectors so every set of transfers goes thru a sleeve .. I use pasty plates to stretch them out

I love the sectioned plates but yea you are limited space wise.




How many generations back do you keep?  So far I've kept everything, but as I get further along I'm thinking it might not make sense to keep them too far back, because if the goal is to get isolates to test, there might not be reason to keep a plate that I've already taken transfers from.

Of course, you're talking about going back to previous generations, I'm just wondering if, rather than going through hundreds of disposable petris, it might be worth putting together a few rotating sets of pastyplates.  :smile:





Oh tottally dude, you save a lot of money that way! I have a mini fridge for cultures so I just kept throwing all the older generations in stacks in there. But once your next set of plates has grown out a little and you can see theyre clean then yea no real reason to hang on to the older plates for long.

Sometimes Ill pull one of the earlier plates out to knock up spawn but for the most part they just sit around until I clean them out haha

But yea, rotating pasty plates is great. I like to store my cultures on disposable plates though, they stack better


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Offlinemushpunx
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Re: Post Your Cultivation Picture of The Day [Re: Pastywhyte]
    #22610801 - 12/03/15 09:36 PM (8 years, 5 months ago)

Quote:

Pastywhyte said:
Bring the nutes back up to 2% :thumbup: IMO when using low nutes to stimulate a culture its better to fluctuate between low and normal rather just low.





Ah right on!
Ill take your advice and start mixing things up a bit


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OfflineSivarted
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #22610858 - 12/03/15 09:47 PM (8 years, 5 months ago)

Quote:

mushpunx said:
Oh tottally dude, you save a lot of money that way! I have a mini fridge for cultures so I just kept throwing all the older generations in stacks in there. But once your next set of plates has grown out a little and you can see theyre clean then yea no real reason to hang on to the older plates for long.

Sometimes Ill pull one of the earlier plates out to knock up spawn but for the most part they just sit around until I clean them out haha

But yea, rotating pasty plates is great. I like to store my cultures on disposable plates though, they stack better




The one thing that's a little tough for me with the pasty plates (and agar in general for that matter) is that I'm working in a SAB and visibility is already pretty limiting, which is an even bigger issue with the pastyplates.

Maybe I should just build myself a flowhood for christmas.  Or figure out a better SAB than a plastic tote that's not nearly big enough anyway.  Haha

Bet I could throw together something out of plexiglass that would be a lot better for precision work

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InvisibleFantastic Mr. Fox
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Re: Post Your Cultivation Picture of The Day [Re: mushpunx]
    #22610859 - 12/03/15 09:47 PM (8 years, 5 months ago)

Forgot about this tray :facepalm:


Jalisco, Mexicana MDLC (7days since nocc)


Chicon Nindo, Mexicana MDLC (7days since nocc)


NW King Oyster MDLC (7days since nocc)


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OfflineYaMoonSun
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Re: Post Your Cultivation Picture of The Day [Re: Fantastic Mr. Fox]
    #22610880 - 12/03/15 09:51 PM (8 years, 5 months ago)

:awegroove:

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