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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Tic Tac Toe Agar (Tiger Drop 2.0) 14
#22601023 - 12/01/15 07:45 PM (8 years, 1 month ago) |
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If you have started using agar and playing with my "easy agar tek", and doing the "Tiger Drop", you might be looking at stepping your game up. The Tiger drop is kickass but, sometimes I want more than one massive nock point. The Tic Tac Toe drop is the solution for you. Basically turns a plate from 1 nock point into 9, all it requires is that you have a clean plate, and a sterilized blade.
The procedure is simple. First you need a colonized mini round no pour plate. Then in either a SAB or flowhood stream, take the round, remove the top and with your sterilized blade make 4 cuts in the agar, in a tic tac toe pattern. Go from edge to edge. Then perform the Tiger Drop with the plate.
The agar is now in 9 still pretty significant chunks. You should see very rapid colonization, especially good for slower colonizers. I like to shake it up real good still. Get the wedges dispersed.
Half Gallons with tic tac toe

Shake em and spread em out a bit

Sometimes a wedge or two may fall off the rim. This is no biggie, just let it go. 7 good size chunks will still colonize pretty fast.
I like to use this for my half gallon jars and spawn em to minis. One culture one jar one sub. Great for quick testing and not needing to expand. They are also really good to go with to pint masters. A pint master with this will colonize very quickly.
I will be fleshing this out some more so consider this a work in progress. . .
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Pastywhyte] 1
#22601062 - 12/01/15 07:49 PM (8 years, 1 month ago) |
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When I first read this I thought you meant Tic Tac Toe as in cutting X's & O's out of the media. lol
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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LOL. I will be updating this with some more pics and a diagram of what a tic tac toe box looks like
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Pastywhyte] 1
#22601113 - 12/01/15 07:55 PM (8 years, 1 month ago) |
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What do you mean by Tic Tac Toe box?
Srry I'm a novice && feeling a bit impatient.. 
Btw, I like the name it's catchy
-------------------- Giving is all we have, for we're just grateful to be alive

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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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This is the pattern your cuts should take. . . inside the round. Just like tic tac toe I am gonna make up a diagram here soon to update the OP with.
Like tic tac toe . . .
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Pastywhyte] 1
#22601171 - 12/01/15 08:11 PM (8 years, 1 month ago) |
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Oh ok! I see now. Thanks for clearing that up.
I can see the advantages when compared to cutting the media in a pie fashion. Greater surface area, equally sized wedges, && I bet the squares don't fall apart and break during transfer like the pie cuts do.  Ive always favored cutting my wedges like squares rather than a wedge.
Your on a roll, Pasty!
-------------------- Giving is all we have, for we're just grateful to be alive

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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Pastywhyte] 1
#22601194 - 12/01/15 08:16 PM (8 years, 1 month ago) |
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Great idea Pasty!! I have a fully colonized plate of pan cyan mycelium(2nd generation), I'll try this out soon with a new batch of WBS jars.
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SynKyd
ctrl-alt-delite



Registered: 09/27/13
Posts: 1,554
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Psilosoulful] 1
#22601437 - 12/01/15 09:04 PM (8 years, 1 month ago) |
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Why not a * pattern that centered on your growth? Just seems like you could cut that into more than 9 segments and have many more noc points if you're going to bring a scalpel vector to the game.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: SynKyd] 2
#22601471 - 12/01/15 09:09 PM (8 years, 1 month ago) |
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Cut it into as many as you like, but straight lines are quick and easy to cut, and 9 wedges are plenty, so I cut it this way. I can make 4 cuts in about 2 seconds and tiger drop with ease allowing for minimal exposure. I guarantee that no one using petris can drop 9 wedges with a single motion.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Pastywhyte] 2
#22601513 - 12/01/15 09:16 PM (8 years, 1 month ago) |
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I've been doing this for awhile! Except its more like a fricken omok board. I also like to hold the dish on its side so nothing is over any media while cutting.
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Mad Season] 1
#22601522 - 12/01/15 09:18 PM (8 years, 1 month ago) |
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Mad Season] 1
#22601720 - 12/01/15 09:46 PM (8 years, 1 month ago) |
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Quote:
Mad Season said:
I've been doing this for awhile! Except its more like a fricken omok board. I also like to hold the dish on its side so nothing is over any media while cutting.
Hell yeah, gotta have proper sterile techniques!
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,158
Loc: rural ghetto
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Psilosoulful] 1
#22601779 - 12/01/15 09:56 PM (8 years, 1 month ago) |
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Thanks again pasty!
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Sivarted
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Registered: 08/22/15
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: pablokabute] 1
#22601826 - 12/01/15 10:05 PM (8 years, 1 month ago) |
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You have just inspired me to try this with the couple pasty plates I still have chilling in the fridge. I dropped an APE wedge on a couple quarts last night, but I'm anxious to try those little fuckers, would be awesome to get a couple quarts colonized *fast*.
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insanemike
Registered: 02/23/14
Posts: 4,272
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: pablokabute] 1
#22601840 - 12/01/15 10:07 PM (8 years, 1 month ago) |
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I have been doing something similar but I just cut the agar or cornmeal mush like a pizza.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 9 days, 22 hours
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: pablokabute] 1
#22601865 - 12/01/15 10:12 PM (8 years, 1 month ago) |
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I like it. I do this too, except I just fork all the squares on my scalpel blade and drop them in at once or in 2 drops.
A full plate in a pint master is way fast!
If Im just innoculating normal quarts I just drop a 1/4 wedge in each jar
Nice tek!
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 Amateur Mycologists United AMU Q&A
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Achillita
Back to the basics



Registered: 05/26/14
Posts: 4,565
Last seen: 3 years, 8 days
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: insanemike] 2
#22601873 - 12/01/15 10:13 PM (8 years, 1 month ago) |
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: Achillita] 2
#22601940 - 12/01/15 10:25 PM (8 years, 1 month ago) |
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Tick tack toe? that like some Canadian game you play on paper?
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: bodhisatta] 1
#22601945 - 12/01/15 10:26 PM (8 years, 1 month ago) |
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It's not just Canadian, it's universal.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Re: Tic Tac Toe (Tiger Drop 2.0) [Re: bodhisatta] 1
#22601949 - 12/01/15 10:27 PM (8 years, 1 month ago) |
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Quote:
bodhisatta said: Tick tack toe? that like some Canadian game you play on
Joking right? 
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (12/01/15 10:49 PM)
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