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natzyshroomer
Star gazer
Registered: 12/01/12
Posts: 405
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Syringes and agar plates
#22590703 - 11/29/15 02:12 PM (8 years, 3 months ago) |
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I was looking for the simple questions thread to ask my question. I am getting into agar if one drop is equal to .05ml, in theory they estimate thats 20 drops to one ml. So one syringe could inoculate 200 agar plates? I must be high
-------------------- All submitted posts are by Someone Who Isn't Me and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit
Edited by natzyshroomer (11/29/15 02:13 PM)
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Machiavelliavore
Vermiculite Hater
Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 5 months, 16 days
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I'd say about the smallest drop I can get is about 1/8th of a ml. Still, you can inoculate a shitton of plates with one drop. A lot of people like putting a drop on a sterile swab and wiping it on the plates to avoid water running all over the place.
See eatyualive's sterile swab thing. Suffice it to say, individually wrap que-tips in foil, put'm in a jar, and PC them for a bit.
-------------------- I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube." No, this does not look right...
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Mycologist217
Frank's Disciple
Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 5 hours
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Made these with sterile swabs that took 3 drops solution to the tip.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
Edited by Mycologist217 (11/29/15 02:22 PM)
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DrCrumbs
Alpha Blue
Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 4 months
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I prefer dropping a PC'ed piece of cotton (from a q-tip, removed a head of time) or a sterile filter disc in the center of the plate and adding a drop of MS solution.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Quote:
natzyshroomer said: I was looking for the simple questions thread to ask my question. I am getting into agar if one drop is equal to .05ml, in theory they estimate thats 20 drops to one ml. So one syringe could inoculate 200 agar plates? I must be high
You might be high... But you are more or less correct...you can grow an infinite amount of shrooms from that one drop if you play your cards right.
-------------------- Q&A US vs. THEM The more I learn, the less I know.
Edited by LocN9ne (11/29/15 03:07 PM)
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natzyshroomer
Star gazer
Registered: 12/01/12
Posts: 405
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Re: Syringes and agar plates [Re: LocN9ne]
#22594126 - 11/30/15 10:51 AM (8 years, 3 months ago) |
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:o goodness I think I might need to learn master slants as well
-------------------- All submitted posts are by Someone Who Isn't Me and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit
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Machiavelliavore
Vermiculite Hater
Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 5 months, 16 days
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You have plenty of time to figure that out once you actually find a culture worth keeping There's also storage in distilled water, which is what I expect to be doing. Sound way easier. I don't wanna buy popsicles.
-------------------- I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube." No, this does not look right...
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natzyshroomer
Star gazer
Registered: 12/01/12
Posts: 405
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This is going to need a farm LOL JK
-------------------- All submitted posts are by Someone Who Isn't Me and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit
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