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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineYaMoonSun
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Registered: 10/23/14
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Polystyrene Petri Heat Resistance
    #22528015 - 11/15/15 03:30 PM (8 years, 3 months ago)

I should be able to steam sterilize these things on the stove-top without a PC, right? I'm scared to pour, seems impractical given my current setup. Would rather pour/stack/sterilize/swap/cool/repeat. :shrug:

Wiki says Melting Point ~ 240 °C (464 °F; 513 K


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OfflineYaMoonSun
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Re: Polystyrene Petri Heat Resistance [Re: YaMoonSun]
    #22528040 - 11/15/15 03:38 PM (8 years, 3 months ago)

Then again, the bag say they're disposable, and they are a bit thin as well.. I just contaminated one for sure. Guess I'll just give it a go and get back to you.


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Offlineblackout
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Re: Polystyrene Petri Heat Resistance [Re: YaMoonSun]
    #22528056 - 11/15/15 03:45 PM (8 years, 3 months ago)

wiki also says

Quote:

As a thermoplastic polymer, polystyrene is in a solid (glassy) state at room temperature but flows if heated above about 100 °C, its glass transition temperature




I heated some before and am pretty sure they deformed very badly before boiling point.

I have tried cold sterilization (or rather sanitisation) with them recently but got contams. I am going to try it again though as I was not taking all the precautions I could.

Some have done this with peroxide, I was trying it with a bleach & vinegar mixture.


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OfflineYaMoonSun
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Re: Polystyrene Petri Heat Resistance [Re: blackout]
    #22528105 - 11/15/15 04:02 PM (8 years, 3 months ago)

*gulps*
Well I made too much agar solution and my minor OCD and great hash convinced me to pour three more plates. So.. :shrug:

I shall get back to you in about 45 minutes, and if they aren't melted, then.. beginner's luck. (First time using a real petri)

Bleach and vinegar huh? Play out okay?


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Offlineblackout
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Re: Polystyrene Petri Heat Resistance [Re: YaMoonSun]
    #22528293 - 11/15/15 04:59 PM (8 years, 3 months ago)

Quote:

YaMoonSun said:
Bleach and vinegar huh? Play out okay?


They contamed, but I was very careless in making them. I soaked in the bleach & vinegar solution and put them on a heat mat with the lids on to dry out. I then lifted the lids the next day and injected hot agar.

So contams could have got in during drying or injecting the agar. My new plan is to soak them and put them in a filtered container. Then heat the container for a few days to draw the moisture off, this also should degrade the bleach.

I am testing it out in a container for LCs, as LCs are prone to contams it should show me if it works pretty quick. So I have a 5L container that had bleach & vinegar in it, I swished it around a few times to cover all the insides. I drained it by pouring the solution out of the filter, which should sanitise it too. I then put this on a heat mat and it was pretty much dried out after a few days. I have a jar of LC with no growth to add to this, and see if it contaminates.


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OfflineYaMoonSun
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Re: Polystyrene Petri Heat Resistance [Re: blackout]
    #22528331 - 11/15/15 05:06 PM (8 years, 3 months ago)

You were right, RIP Petri Dishes :lol:
I think those pasty plates are the way to go. Dunno why I wasted my time on these. I knew I didn't want to pour. :shrug:


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OfflinecronicrFacebook
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Re: Polystyrene Petri Heat Resistance [Re: blackout]
    #22528334 - 11/15/15 05:08 PM (8 years, 3 months ago)

dude there sterile don't sweat pouringit isn't hard to do at all, it seems risky till ya do it


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OfflineYaMoonSun
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Re: Polystyrene Petri Heat Resistance [Re: cronicr]
    #22528344 - 11/15/15 05:10 PM (8 years, 3 months ago)

I'm sure I'll give it a go when I tire of making bootleg petris from random things in my kitchen.


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OfflinecronicrFacebook
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Re: Polystyrene Petri Heat Resistance [Re: YaMoonSun]
    #22528348 - 11/15/15 05:11 PM (8 years, 3 months ago)

:lol:


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Offlinemushpunx
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Re: Polystyrene Petri Heat Resistance [Re: cronicr]
    #22528354 - 11/15/15 05:13 PM (8 years, 3 months ago)

Yea man pouring plates isn't even hard the first time around.

You can buy by the case of 500 and theyre pretty cheap too.



  I use Pasty Plates and mason jars all the time though, Im always running out of plates


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Offlineblackout
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Re: Polystyrene Petri Heat Resistance [Re: YaMoonSun]
    #22528362 - 11/15/15 05:17 PM (8 years, 3 months ago)

To test plastics I just boil them in a bowl of water in a microwave, if it gets overly floppy its no good.

The disposable petris are sterile in the sleeve, I presumed you were reusing ones or that they were otherwise not sterile anymore.

I had success injecting agar into sterile disposable petris while they were still in the sleeve of 10.  Injecting through the plastic and lifting the petri lid with the needle and injecting hot agar. I would advise starting from the bottom dish upwards, I did it the other way around and my upper plates were tilting and risked agar spilling out. Also when I started from the top down when I got to the bottom I was lifting a stack of now heavy plates. This is like using the sleeve as a SAB. Disposable petri are very light so if you start bottom up it will be easy enough to lift them. You can pinch the lid with your other hand which can help lift the lid.

Another idea I had was to heat a needle on a syringe full of hot agar and poke it into the lid of the dish and inject, burning a small hole in the lid, and have little bits of tape set aside which would be stuck over the hole.


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OfflineYaMoonSun
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Re: Polystyrene Petri Heat Resistance [Re: blackout]
    #22528412 - 11/15/15 05:28 PM (8 years, 3 months ago)

Nope, they were in the sleeve still. I honestly thought at first they put the petris into the sleeve in a normal fashion, so I opened it from the bottom hopping to be clever, but as it turns out, they too were clever and actually put the petris in upside down. :facepalm3:

I looked at some Pyrex petris, they're expensive, but seems worth the value if you plan on making hundreds of transfers anyways


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Offlinemushpunx
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Re: Polystyrene Petri Heat Resistance [Re: blackout]
    #22528443 - 11/15/15 05:34 PM (8 years, 3 months ago)

Hah I admire the ingenuity but I think its just easier to pour :lol:


I find some plates are better than others though. The ones I have right now kinda bend when I wrap them.
Ive had others that seemed so tough they might even make it thru a quick PC cycle

Someday I might grab some glass ones :shrug: get fancy




I wipe the sleeve down with ISO and move it into SAB or in front of my hood. Ill flip it over and cut the bottom w my scalpel, then holding the stack in place flip it back over and pull the sleeve up and off .

I break it into 2 stacks of ten, and pour from the bottom up. Then I put into fresh z iplocks in stacks of 4 or wrap w cling wrap. Then parafilm after inoculation


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InvisiblebodhisattaMDiscordReddit
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Re: Polystyrene Petri Heat Resistance [Re: mushpunx]
    #22528675 - 11/15/15 06:39 PM (8 years, 3 months ago)

pouring plates is by far the easiest shit ever.


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Offlinemushpunx
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Re: Polystyrene Petri Heat Resistance [Re: bodhisatta]
    #22529053 - 11/15/15 07:44 PM (8 years, 3 months ago)

Yea man I dunno why people get so intimidated by it. Its really cake work


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InvisiblebodhisattaMDiscordReddit
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Re: Polystyrene Petri Heat Resistance [Re: mushpunx]
    #22529358 - 11/15/15 08:29 PM (8 years, 3 months ago)

if you want to sterilize polystyrene dishes without heat you can. gama or Eto gas (Anprolene)

Sterilization is a misnomer though, you can't chemically sterilize anything

EtO has long been recognised as a very effective sterilant. It remains the most common form of sterilization worldwide for single use devices. The Andersen system makes this tried and trusted technology available on a small scale. The Sterility Assurance Level (SAL) of 10-6 which is the global standard for sterilization, as opposed to disinfection, is available through the Andersen system to small operations and for small loads.

10e-6 is 6 logs reduction, essentially sterile, it's really just super sanitation. same thing that happens to our grains in the PC


Edited by Trusted cuItivator (11/15/15 08:35 PM)


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Offlineblackout
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Re: Polystyrene Petri Heat Resistance [Re: blackout]
    #22586556 - 11/28/15 01:54 PM (8 years, 2 months ago)

Quote:

blackout said:
I am testing it out in a container for LCs, as LCs are prone to contams it should show me if it works pretty quick. So I have a 5L container that had bleach & vinegar in it, I swished it around a few times to cover all the insides. I drained it by pouring the solution out of the filter, which should sanitise it too. I then put this on a heat mat and it was pretty much dried out after a few days. I have a jar of LC with no growth to add to this, and see if it contaminates.



I added the new clear "nutrient broth" (i.e. fresh uninoculated LC) to this 5L container and have had it at about 23C, not sure exactly when but long enough for it to have contaminated, I am pretty sure over a week. It is still looking perfectly clear. So either the residual bleach vinegar mixture has sterilized the nutrient broth and will not produce any growth, or else it has worked as planned.

I have a new LC growing which will be put into the 5L container soon to see if new growth happens.

Even if the new growing LC does not multiply I think the method is worth further testing. If any residual liquid or dried chemicals on the surface kill new growth you might be able to pour a layer of agar on this, and then pour a second layer on top, so the top layer should have no "sterilizing chemicals" dissolved in it.


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