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Offlinebuttermycoface


Registered: 02/03/15
Posts: 130
Last seen: 2 years, 9 months
Went to ER after SAB lit up * 1
    #22586071 - 11/28/15 12:01 PM (8 years, 2 months ago)

So I preped everything. Poured agar in petri dishes. Spray down inside of SAB with ISO and wipe sterile swap down with a soaked cloth. Opened the swab wraper and as i strike the lighter outside the SAB to sterelize needle, everything lights up in my face.

Any have a tek on how to sterilize glass petri dishes?


Edited by buttermycoface (11/28/15 03:07 PM)


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
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Re: Went to ER after SAB lit up [Re: buttermycoface]
    #22586085 - 11/28/15 12:05 PM (8 years, 2 months ago)

Pretty sure you were supposed to use lysol inside the SAB, and the only things you wipe down with ISO are your arms, hands, and maybe the jars. Sounds like you used WAY too much, and possibly 90% too. Sorry to hear about your accident :facepalm3:

You can sterilize those using steam for roughly 2h45m if you don't have a pressure cooker. Just don't let the water run dry or you'll have to start from scratch.


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OfflineMoxyOx
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Registered: 10/08/10
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Re: Went to ER after SAB lit up [Re: YaMoonSun]
    #22586093 - 11/28/15 12:08 PM (8 years, 2 months ago)

What kind of glass are they? Make sure they can withstand the thermal increase/decrease. They need to be borosilicate, although I recall another form of glass that can withstand the temperatures albeit not as well.

You don't need a 2 hour and 45 min cycle to sterilize petri dishes. I did mine for 30 minutes at 15 PSI and they are fine.

Shit, even freaking spawn(oat, wheat, WBS) should be 1 hour and 30.


--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


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OfflineYaMoonSun
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Registered: 10/23/14
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Re: Went to ER after SAB lit up [Re: MoxyOx]
    #22586145 - 11/28/15 12:20 PM (8 years, 2 months ago)

Quote:

if you don't have a pressure cooker.



:lol:


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Offlinebuttermycoface


Registered: 02/03/15
Posts: 130
Last seen: 2 years, 9 months
Re: Went to ER after SAB lit up [Re: MoxyOx]
    #22586161 - 11/28/15 12:23 PM (8 years, 2 months ago)

It was 70% with 5% ethanol air sterilizer. I just wraped them in tinfoil and sterilized at 15psi for 20 mins. I think they came out nice, apart from the doctor bill, i have to go back to the wound care clinic monday morning. The glass withstood the PC easily.


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Invisible36fuckin5
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Registered: 08/11/03
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Loc: Diving into Mystical Territori...
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Re: Went to ER after SAB lit up [Re: buttermycoface]
    #22586170 - 11/28/15 12:25 PM (8 years, 2 months ago)

This is why you use bleach water or soapy water in your SAB. Alcohol is used on you, your jars/plates/etc and your tools and nothing else.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OnlineBaby_Hitler
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Re: Went to ER after SAB lit up [Re: buttermycoface]
    #22586177 - 11/28/15 12:26 PM (8 years, 2 months ago)

Why do people still use iso and Lysol? a 10:1 water:bleach solution is all you will ever need.

10:1:1 water:bleach:vinegar is even better. DO NOT mix vinegar with full strength bleach. Mix the bleach into the water, then the vinegar.

Quote:

RogerRabbit said:
You can mix bleach and vinegar if you dilute the bleach with water first, as you should anyway.  In fact, adding vinegar to bleach/water makes it much more effective. http://www.eurekalert.org/pub_releases/2006-02/asfm-vik021306.php

Soap and water are all you need for a fruiting chamber.
RR




--------------------
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InvisiblebodhisattaMDiscordReddit
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Re: Went to ER after SAB lit up [Re: YaMoonSun]
    #22586181 - 11/28/15 12:27 PM (8 years, 2 months ago)

you use iso to wipe the outside of jars and your hands/gloves and the handle of the scalpel. the inside of the SAB never has ISO or any kind of alcohol.... it's a bomb as you found out.

you can spray the inside of the SAB with plain dilute soap water (500ml with 1 drop of dish soap) this will knock down contaminants and make the walls wet so contaminants will stick.

contamination falls straight down in a SAB, sterile technique takes advantage of this to work in an environment that isn't sterile at all but you can keep your work sterile.


To sterilize glass petri dishes, first wash them until they're surgically clean. no reside or debris on them at all. 100% like they came from the factory clean glass.

wrap them in foil so when you take them out of the oven they're in a convenient sterile package.

put them in your oven at 320F for 2 hours or 340F for one hour. measure with a oven thermometer and don't rely on your readout from the oven itself.


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Offlinespacechildo
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Re: Went to ER after SAB lit up [Re: buttermycoface]
    #22586186 - 11/28/15 12:28 PM (8 years, 2 months ago)

bleach :puke: used that once, never again. soapy water is all I ever use.

also pretty sure that pic is a joke. looks like someone dressed up as a mummy for halloween :shrug:


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OfflineYaMoonSun
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Registered: 10/23/14
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Re: Went to ER after SAB lit up [Re: spacechildo]
    #22586199 - 11/28/15 12:30 PM (8 years, 2 months ago)

That air sterilizer sounds like the cause honestly. The last think you want is an airborne combustible element.

Quote:

used that once, never again



Why so?


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Offlinespacechildo
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Re: Went to ER after SAB lit up [Re: YaMoonSun]
    #22586212 - 11/28/15 12:33 PM (8 years, 2 months ago)

Quote:

YaMoonSun said:
That air sterilizer sounds like the cause honestly. The last think you want is an airborne combustible element.

Quote:

used that once, never again



Why so?




because it smells like shit in my entire apt afterwards. soapy water dont.


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InvisiblebodhisattaMDiscordReddit
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Re: Went to ER after SAB lit up [Re: spacechildo]
    #22586232 - 11/28/15 12:39 PM (8 years, 2 months ago)

Quote:

MoxyOx said:
What kind of glass are they? Make sure they can withstand the thermal increase/decrease. They need to be borosilicate, although I recall another form of glass that can withstand the temperatures albeit not as well.

You don't need a 2 hour and 45 min cycle to sterilize petri dishes. I did mine for 30 minutes at 15 PSI and they are fine.

Shit, even freaking spawn(oat, wheat, WBS) should be 1 hour and 30.



it only takes 15m at 15psi to sterilize agar(if it started out as all powdered ingredients) and 15m at 15psi to sterilize insturments like glass and scalpels. (they must be 100% clean first before autoclave, surgically clean)




Quote:

Baby_Hitler said:
Why do people still use iso and Lysol? a 10:1 water:bleach solution is all you will ever need.

10:1:1 water:bleach:vinegar is even better. DO NOT mix vinegar with full strength bleach. Mix the bleach into the water, then the vinegar.

Quote:

RogerRabbit said:
You can mix bleach and vinegar if you dilute the bleach with water first, as you should anyway.  In fact, adding vinegar to bleach/water makes it much more effective. http://www.eurekalert.org/pub_releases/2006-02/asfm-vik021306.php

Soap and water are all you need for a fruiting chamber.
RR






acidified bleach is the best chemical sanitizer for this hobby
bleach is a base. when you dilute it 1:10 with water. water being pH 7 you are acidifying the bleach. bleach works better at a lower pH (more acidic) than it does in the bottle at bottle strength.
one part bleach to ten parts water(with or without vinegar) is bar none the best sanitation chemical you can get for what we do.
it's better than alcohol/

I personally use alcohol for wiping things because I hate bleach.

sanitizers are a crutch anyway you shouldn't be relying on them as they do not sterilize anything. your technique and skill dictates your success not the spray bottle.


Quote:

spacechildo said:
Quote:

YaMoonSun said:
That air sterilizer sounds like the cause honestly. The last think you want is an airborne combustible element.

Quote:

used that once, never again



Why so?




because it smells like shit in my entire apt afterwards. soapy water dont.



exactly


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Invisiblewowimflabbergasted
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Re: Went to ER after SAB lit up [Re: buttermycoface] * 3
    #22586238 - 11/28/15 12:41 PM (8 years, 2 months ago)

Quote:

buttermycoface said:
So I preped everything. Poured agar in petri dishes. Spray down inside of SAB with ISO and wipe sterile swap down with a soaked cloth. Opened the swab wraper and as i strike the lighter outside the SAB to sterelize needle, everything lights up in my face.





:hahathatsfunny:


--------------------
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Invisibleazur
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Registered: 04/21/12
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Re: Went to ER after SAB lit up [Re: buttermycoface] * 1
    #22586245 - 11/28/15 12:43 PM (8 years, 2 months ago)

At least yo name matches yo face


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Offlineblackout
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Re: Went to ER after SAB lit up [Re: bodhisatta]
    #22586260 - 11/28/15 12:48 PM (8 years, 2 months ago)

Quote:

MoxyOx said:
What kind of glass are they? Make sure they can withstand the thermal increase/decrease. They need to be borosilicate, although I recall another form of glass that can withstand the temperatures albeit not as well.



If you heat glass gently/slowly and cool it gently it should not crack. So do not go putting petris onto a rack above a pot of steaming water.


Quote:

bodhisatta said:
the inside of the SAB never has ISO or any kind of alcohol.... it's a bomb as you found out.


I usually have my tools presterilized and covered in foil or more recently in copper covers. This means I have no need for flaming, I use mulitple tools so my hands do not have to leave the SAB, I just find it easier. But even if alcohol was not flammable I would not use it. I used it before but since alcohol is more volatile it created condensate on the tubs so much harder to see what was going on.


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Offlinebuttermycoface


Registered: 02/03/15
Posts: 130
Last seen: 2 years, 9 months
Re: Went to ER after SAB lit up [Re: spacechildo]
    #22586263 - 11/28/15 12:48 PM (8 years, 2 months ago)

They put the dressings on last night that photo was after wake and bake this morning, otw to the hospital for a dressing change. I bernt pretty bad dude.


Check my neck, my arms are the worst but i dont have a photo as its still in dressing. No arm hair no face hair and burnt my fringe gone.


Notice the arm hair


Edited by buttermycoface (11/28/15 03:08 PM)


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Invisiblenobody83
stranger danger
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Registered: 03/15/14
Posts: 1,486
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Re: Went to ER after SAB lit up [Re: azur]
    #22586271 - 11/28/15 12:50 PM (8 years, 2 months ago)

Wow the funniest thing ive seen in weeks. Its funny when its not my ass on the line
:lolsy:


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Offlinespacechildo
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Re: Went to ER after SAB lit up [Re: buttermycoface]
    #22586275 - 11/28/15 12:51 PM (8 years, 2 months ago)

survival of the fittest man... sorry to be the one to tell you :rolleyes:


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Invisibleazur
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Re: Went to ER after SAB lit up [Re: blackout]
    #22586276 - 11/28/15 12:51 PM (8 years, 2 months ago)

I have always pc sterilized many tools and rarely need to flame them


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisiblebodhisattaMDiscordReddit
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Re: Went to ER after SAB lit up [Re: azur]
    #22586285 - 11/28/15 12:53 PM (8 years, 2 months ago)

I use one scalpel and do multiple flames. but I have disposable inoculation loops.


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