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InvisibleJared
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Hate to ask.
    #2258311 - 01/21/04 01:38 AM (12 years, 10 months ago)

I tried searching, but got no responses in regards to this Q. Has anyone tried innoculating at only one spot, namely the center. Insted of introducing a 1/3 or so cc of innoculant, saturating the center of the (brf) cake with 1cc.. The reason I ask is because it seems like it would speed up colonization, as the mycelium could travel in all directions as opposed to being blocked in more than 50% of directions by glass.. Also, as only one injection would be required the risk of contamination would be reduced, along with that the spot where the needle was introduced would be so saturated with spores that the mycelium would be able to get a foothold more quickly against any possible contams, and would grow faster (in my exp more spores = faster colonization.. to an extent) The only downside to this I can think of, is that contams would take longer to rear their ugly heads, however, I assume contams grow more quickly than mycelium and would be apparent before the rest showed the mycelium peeking through.. also, if it contamed would the mycelium be able to grow through? Or would it centralize against the green and die off before reaching the surface (glass)

Sorry for the quality of these drawings, I just formated and don't have PS installed yet. Thanks for all responses. :smile:





-Jared


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Invisiblesimplemachine
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Re: Hate to ask. [Re: Jared]
    #2258333 - 01/21/04 01:44 AM (12 years, 10 months ago)

Best thing you can do is have a shakeable substrate...if a cake contams it's more than likely not going to fight off the contam. In this case just toss it if it contams!


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InvisibleJared
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Re: Hate to ask. [Re: simplemachine]
    #2258347 - 01/21/04 01:48 AM (12 years, 10 months ago)

Alas, I have no PC and am restrained to BRF cakes.. for now.. (Waiting on an AllAmerican! :laugh: :laugh: :laugh:) Of course I toss a cake if it contams.. thats not really the question at hand, I meant  would the mycelium make a run for it.. to colonize as much as it could, or would the trich quickly kill it off.. thus never letting the mycelium reach the surface.. an easy way to tell if a cake is contamed.. if all others are peeking through and a few are not.. I dunno its late, I'm probably making no sense.  :nut:


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Invisiblesimplemachine
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Re: Hate to ask. [Re: Jared]
    #2258357 - 01/21/04 01:51 AM (12 years, 10 months ago)

I'm working on a shakeable variation of the pf tek right now i will post it in a few minutes...



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InvisibleJared
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Re: Hate to ask. [Re: simplemachine]
    #2258362 - 01/21/04 01:52 AM (12 years, 10 months ago)

I have no clue how that will work.. I can't even picture it... and usually I'm a picturing stuff kinda guy. Please post soon :smile:


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OfflineParanoidSchizoid
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Re: Hate to ask. [Re: Jared]
    #2258404 - 01/21/04 02:05 AM (12 years, 10 months ago)

I have done what you're describing. I inoculated in the middle, but I also squirted inoculant across the top surface, since this is where the contams enter. Once the spores germinated, it would form somewhat of a mycelial barrier of protection. Then the mycelium can colonize the substrate from top to bottom and from the center outward.


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Growing conspiracy
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Anonymous

Re: Hate to ask. [Re: Jared]
    #2258551 - 01/21/04 02:36 AM (12 years, 10 months ago)

I just do 5 innoc points 4 on the side and on in the middle seems to colinize faster this way I dunno about one but 5 is the way to go


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Anonymous

Re: Hate to ask. [Re: Jared]
    #2258983 - 01/21/04 06:47 AM (12 years, 10 months ago)

for wbs i only innoc from one hole by spraying in a circular motion.
i reckon for the brf tek it would be better to stick with the 4 holes as this makes 4 starting points which will meet up much quicker instead of just one spot.


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OfflineAnnoA
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Re: Hate to ask. [Re: Jared]
    #2259093 - 01/21/04 09:07 AM (12 years, 10 months ago)

>it seems like it would speed up colonization

The contrary is the case, I tried it, it takes considerably longer.

Inoculate on 4-5 points at the perimeter.
Another drawback of inoculating it in the middle is that you don?t see if you have clean growth or contamination.


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Offlinetoikey
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Re: Hate to ask. [Re: Jared]
    #2259252 - 01/21/04 11:21 AM (12 years, 10 months ago)

not only those reasons...
if you read the pf tek i think it says it improves distribution of spore solution when the needle is touching the glass... otherwise pieces of substrate could potentially block the tip, wasting spores and leave the jar for the germs :smile:
i think this is why my first ever attempt turned into smellyness


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InvisibleTheHateCamel
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Re: Hate to ask. [Re: Jared]
    #2259266 - 01/21/04 11:27 AM (12 years, 10 months ago)

'


Edited by TheHateCamel (12/08/07 02:13 AM)


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InvisiblePrisoner#1M
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Re: Hate to ask. [Re: TheHateCamel]
    #2259343 - 01/21/04 12:05 PM (12 years, 10 months ago)

as anno stated, innoculation in the center prevents you from noticing the contam...you'll see it soon enough though....


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OfflineAnnoA
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Re: Hate to ask. [Re: toikey]
    #2259381 - 01/21/04 12:33 PM (12 years, 10 months ago)

>i think it says it improves distribution of spore solution when the needle is touching the glass...

Yes, another reason to inoculate towards glass, this way the spore suspension drop slides down the jar and inoculates the substrate on the whole length from bottom to top.


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Offlinealienated
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Re: Hate to ask. [Re: Jared]
    #2260783 - 01/21/04 08:42 PM (12 years, 10 months ago)

i inject in 4 spots, 3 on the sides, and 1 down the middle. as you inject in the middle, push all the way down and as you're injecting starting to take the needle out. that's what i do and i get nice colonization. hope that helps.

pz


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-m.

I dont even grow these things... I just post for this guy that knows a guy, who's sisters boyfriend dumped about 2 months ago.


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OfflineNickster_154371
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Re: Hate to ask. [Re: alienated]
    #2261115 - 01/21/04 10:45 PM (12 years, 10 months ago)

Hey i like that idea alienated, im def. gonna try that next time!


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Wild Bird Seed

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