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Offlinetree frog
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: labbar]
    #28566843 - 12/03/23 02:26 PM (1 month, 24 days ago)

I'm excited to see the stones that come out of a forgotten box!


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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OfflineGenome794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado]
    #28568275 - 12/04/23 12:50 PM (1 month, 23 days ago)

Quote:

normalperson said:
    are you saying that you do that while mixing with the sub? you are rubbing the spawn and sub together until the spawn is "bare"?




In a word, yes... I throw in the sub and the spawn on top. (I shake my jars enough to get the aroma and the spawn out easily.) I grab a big handful of spawn and sub and just rub it through with my thumb until it has all fallen back into the tub, repeat until mixed. I have a Yeti culture that I can rub until bare and it will colonise 1:4 in 5-6 days.

Quote:

Mycolorado said:
Yep.  They can stop growing and start to bruise.




Oops... I guess I got lucky then. I used the above method with 1:3. Sub is regular CVG with approx 15% composted horse manure (I asked for manure at a local stable and got a manure, horse bedding and some hay compost). They are at 3 days in dub-tubs. And I always have a top coat layer.

The jars that have the big stones are now here:


The other jar is hasn't started busting up the surface yet but fully colonised too.


Back to the waiting game... While I wait. Case at some point? Mist? Bottom water when the sub starts to shrink?

Thanks for all the help794


Edited by Genome794 (12/04/23 01:14 PM)


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Genome794] * 1
    #28568516 - 12/04/23 04:17 PM (1 month, 23 days ago)

1:2 ratio produced the stones below and 2 dry grams of mush from 1 pint spawn.


1:4 ratio from the same culture produced 13 g stones and 40 g dry mush from 1 pint spawn in three flushes.

Sadly shutting down until after the new year so all the stones went into the bin.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Offlinetree frog
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc]
    #28568674 - 12/04/23 06:23 PM (1 month, 23 days ago)

What substrate Rev?  Just coir?  CV?

I'm really liking both the stones and the fruits from Tamps.  I think I've gotten about 8g of fruits so far off a 1:2 millet:CVG MS (ATL 7) shoebox.  Just under a mycoquart of millet (was using those 3/4 quart skinny jars).

That's off two flushes and I picked a lot of fruits early so I wouldn't have to fuck with the deydrator again.

Ate a gram last night and was surprised.  Probably around a level 2.  A lot stronger than the Malabar Coast I grew over the summer (which were overall pretty weak) but also stronger than I remember my B+ grows being (and those were consistently pretty good and occassionally really good).


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Edited by tree frog (12/04/23 06:28 PM)


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: tree frog] * 1
    #28569557 - 12/05/23 09:14 AM (1 month, 22 days ago)

I prefer straight coir for tamps so you don't end up chewing on verm with the stones. However, I like to use some sand in the casing at full colonization. It really seems to help induce pinning in all of my side by side testing.

I like the experience with stones, they did seem more cube-like than the fruit, but that could totally be subjective or set and setting. I have not done enough trials to defend that observation. The fruit definitely give me a more happy and appreciative vibe than cubes, though.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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OfflinePnin
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Registered: 07/18/23
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc]
    #28569567 - 12/05/23 09:21 AM (1 month, 22 days ago)

What kind of sand do you use Rev?


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Offlinetree frog
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc] * 1
    #28569574 - 12/05/23 09:27 AM (1 month, 22 days ago)

Thanks.

I've been planning to switch to coir but I had a bag of CVG already made up in the garage I'm trying to get through.  I'm running some experiments with pseudo-barrier between the casing and the sub (90% shade cloth) to try to get casing stones easier to clean up.  My first experiment was with 70% shade cloth as it's what I had around and they've colonized the casing above the shade cloth without issue.  So hopefully I'll be able to just pull it off when the time comes and get cleaner stones.  We'll see what side of the shade cloth they put the stones on and if it makes a difference or not in a month or two and I'll share the results (I have three variations on the experiment going).

I'll probably switch to sand in my casing and ditch the verm if it's helping a lot with pin sets.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Pnin] * 2
    #28569621 - 12/05/23 09:57 AM (1 month, 22 days ago)

Cool idea. I think someone tried using cheese cloth to make regular cube harvest easier a while back. Never thought about stones.

Quote:

Pnin said:
What kind of sand do you use Rev?



https://www.homedepot.com/p/Quikrete-50-lb-Premium-Play-Sand-111351/100318476

Just plain old sand. I got a life time supply at HD for like 6 bucks. I add a couple of hand fulls per quart of casing before I pasteurize.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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OfflineFungal-Infection
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc]
    #28570887 - 12/06/23 02:23 AM (1 month, 22 days ago)

I know we can’t be exact here, but typically, how long after a grain jar is colonised with Tampanensis will stone production start?

I’m in no rush, I know they take months but I just wondered when they’re likely to start.

Cheers!


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Offlinetree frog
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Fungal-Infection] * 1
    #28570895 - 12/06/23 03:01 AM (1 month, 22 days ago)

What strain of Tamps?  ATL 7 from MS started stoning up very quick.  Less than a week from full colonization.  I've seen jars start making stones before full colonization too.  I probably wouldn't wait longer than two weeks personally based on what I've seen so far from it.

Wild tamps aren't likely to go that fast.  And I imagine some non-domesticated genetics wouldn't like jars at all.

But maybe someone will have a better answer for you in a bit.  I'm only just getting back into growing actives.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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OfflineFungal-Infection
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: tree frog] * 1
    #28570998 - 12/06/23 06:16 AM (1 month, 22 days ago)

Quote:

tree frog said:
What strain of Tamps?  ATL 7 from MS started stoning up very quick.  Less than a week from full colonization.  I've seen jars start making stones before full colonization too.  I probably wouldn't wait longer than two weeks personally based on what I've seen so far from it.

Wild tamps aren't likely to go that fast.  And I imagine some non-domesticated genetics wouldn't like jars at all.

But maybe someone will have a better answer for you in a bit.  I'm only just getting back into growing actives.




Thanks for replying, tree frog.

I have no idea what strain it is.

The vendor I brought it off doesn’t say. There’s just this historical blurb about the discovery of Tampanensis.

Quote:


First discovered by Stephen Pollock outside of Tampa, FL, in 1977. Wanting to avoid a “boring mycological conference”, Pollock decided to go check out some pastures nearby his hotel. Little did he know, he’d discover the illusive species we all know and love today. Tampanensis is the true philosophers stone. Capable of producing sclerotia, also commonly known as truffles, tampanensis are one of the rarest species on earth to find in the wild. Since the first discovery in 1977, the species has been recorded only once, and that was in the state of Mississippi. Every culture being passed around today is off that initial specimen by Pollock.





It was an isolated spore syringe but I’m lead to believe that is tech speak for Liquid Culture? because that’s what was in mine lol

It is a popular and well known trusted vendor but I’m not sure if I’m allowed to name them or not.

This is my test jar which I first inoculated just over 2 weeks ago along with 2 plates. Every grain I can see is covered in fine growth. It stands out to the naked eye but it’s hard to get the lighting right to take a good pic lol





These are my two agar plates





In the last plate, the mycelium has started to develop a dark area which I am hoping is signs it might grow a stone on the plate.


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Offlinetree frog
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Fungal-Infection] * 1
    #28571122 - 12/06/23 07:35 AM (1 month, 21 days ago)

Lol yeah don't name vendors, especially not ones mailing liquid culture (those ain't spores).

Give me a minute and I have some very small stones developing.  I'll get a picture so you know what the babies look like.

Assuming my phone gets a decent picture anyway.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: tree frog] * 1
    #28571143 - 12/06/23 07:51 AM (1 month, 21 days ago)

I circled developing stones in blue.  There's three easily visible stones in that big blue circle.  There's a lot more not circled.  Just felt this was enough to give you an idea what to watch for.



Same image.  Red arrow is pointing to the smallest visible stone.  It looks like just a brown or black dot.



I'm not sure about the culture plate.  I think that's what you want but my plates aren't on contrasted agar and look much different.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Edited by tree frog (12/06/23 08:15 AM)


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: tree frog]
    #28571278 - 12/06/23 09:21 AM (1 month, 21 days ago)

Tree Frog is leading you right.

I have run the "Pollock variety" many times and it can start stoning up before it completely colonizes, a few weeks after or never. It is kind of a crap shoot. I had the best stones in jars on oats. Wheat never wanted to throw stones in jars very well, but would do some in substrate tubs. Millet also gave me nice stones in substrate tubs, but I haven't given them enough time in jars to know if they get stoney there.

Those jars look quite good to my eyes. The second plate looks like Tamps but the first one is confusing. Is it colonized all around the edge and a line to the center? That seems weird (unless you rolled the lc/li around the plate, intentionally or not). I would transfer from both, but keep a close eye on anything from the first plate. Dark areas in tamp plates is more than normal. It is pretty much expected.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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OfflineMelgo
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc] * 6
    #28575126 - 12/08/23 10:40 PM (1 month, 19 days ago)

Little "Mex. A" mudda grow:


I need to dial in my JCM for more FAE as these aren't developing their caps properly...
They aren't dropping spores :facepalm3:


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Offlinetree frog
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado]
    #28575717 - 12/09/23 11:15 AM (1 month, 18 days ago)

Quote:

Mycolorado said:


Obviously I like sand and seed starter which is usually a blend of peat, coir and verm and lime for my casing.  When you say sphag moss, is it moss or peat?  Some folks get the 2 mixed up.  If it’s peat, I’d blend it 50/50 with sand and add a tbsp of lime per cup of mix and pasteurize.  Check my Tampanensis Sand Casing thread if you haven’t seen it.




Sorry to quote such an old post but I'm making up some peat/sand casing.

In the linked post 1 tsp of lime is used.  In the quoted text it says a tbsp.

Also I only have calcium carbonate and hydrated lime (the linked thread says to use gardening lime).

So should I do a tsp of calcium carbonate?  A tbsp?  Skip the hydrated lime yeah?


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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InvisibleMycolorado
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: tree frog]
    #28575782 - 12/09/23 12:29 PM (1 month, 18 days ago)

That was a second ago so not sure which measurement I used but I believe the jiffy was already pH’d so used less per cup.  Use the hydrated lime and keep it off your skin, eyes, etc.  5% per volume of peat.  Check this thread:
https://www.shroomery.org/forums/showflat.php/Number/4358473#4358473


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Offlinetree frog
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado]
    #28575846 - 12/09/23 01:21 PM (1 month, 18 days ago)

Appreciate it.

I've been casing off that thread actually, just first time using sand  I guess it's the same basically just subbing out the verm.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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OfflineFungal-Infection
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc]
    #28576649 - 12/10/23 06:23 AM (1 month, 18 days ago)

Quote:

tree frog said:
I circled developing stones in blue.  There's three easily visible stones in that big blue circle.  There's a lot more not circled.  Just felt this was enough to give you an idea what to watch for.



Same image.  Red arrow is pointing to the smallest visible stone.  It looks like just a brown or black dot.



I'm not sure about the culture plate.  I think that's what you want but my plates aren't on contrasted agar and look much different.




Thank you Tree Frog those pictures are really helpful!!!

I have some “interesting” things happening in my jar. I did my best to take pictures.







Anything here I should be concerned about, do you think?

RE my agar plates, I’m not confident enough to pour my own yet so I buy packs of 10 from a guy on eBay. They are supposedly MEA with Charcoal. I thought a contrasting colour like that might be helpful? These perhaps aren’t the ideal plates for stone producers in terms of nutrient / ratios?



Quote:

ReverendMyc said:
Tree Frog is leading you right.

I have run the "Pollock variety" many times and it can start stoning up before it completely colonizes, a few weeks after or never. It is kind of a crap shoot. I had the best stones in jars on oats. Wheat never wanted to throw stones in jars very well, but would do some in substrate tubs. Millet also gave me nice stones in substrate tubs, but I haven't given them enough time in jars to know if they get stoney there.

Those jars look quite good to my eyes. The second plate looks like Tamps but the first one is confusing. Is it colonized all around the edge and a line to the center? That seems weird (unless you rolled the lc/li around the plate, intentionally or not). I would transfer from both, but keep a close eye on anything from the first plate. Dark areas in tamp plates is more than normal. It is pretty much expected.




Thank you for replying Reverend.

This is my first time with stone producers and despite reading loads, it gets quickly overwhelming so it’s always good to get help from experienced eyes.

It took me a LOT of reading and head scratching to just get to beyond all the sales crap to find what the “base” species were. Atlantis, Hollandia, Utopia etc. Thank goodness for Allan Rockefeller and his work helping narrowing down the IDs

Yeah, I’ve read it can be pot luck if they produce stones.
The way I’m coming at it is, A) If I get just one stone, I’ll clone it and B) If I don’t get any stones, I’ll try to fruit the spawn. So a bit of a win win in a way.

RE the Agar, yeah, on the plate with the growth all round the edges, that was my clumsy hands putting on the parafilm.
I put a small dribble of LC in the centre of the plate, probably a little too much and it ran round the edges lol

I’m so paranoid about contam that I like to work as quickly but deliberately as possible with Agar. I barley lift one end of the agar plate lid any more than 45 degrees, a very gentle push on the plunger and close up the plate.

I don’t like messing about squeezing the syringe to get a tiny droplet. With my hands, that’s when the needle ends up touching shit it shouldn’t lol

Flame the needle, squeeze some LC out too cool it, inoculate the plate and done. No more than 30 seconds. But not rushed. I try to be deliberate and careful.

I’m even more paranoid working with these Tamps.
I found them so hard to obtain and expensive when I did that I don’t wanna screw them up. Previous attempts from print didn’t go well at all. Just contam after contam.


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OfflineFungal-Infection
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Fungal-Infection]
    #28578021 - 12/11/23 03:33 AM (1 month, 17 days ago)

Excuse the double post but… ARRRRGGGGHHH! 😩😫😩😫
This doesn’t look good.

This appeared in my first test jar. It’s, it’s… it’s GREEN 😩🥺







All my other 11 jars seem ok so far and my Agar plates are still clean. So I think I need to look at my grain jar process.


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