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Invisiblethe_chosen_one
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: A.k.a]
    #28513050 - 10/21/23 01:49 PM (3 months, 5 days ago)

Quote:

A.k.a said:
I’ve always heard cincts were barely active, but it’s definitely all hearsay I don’t recall seeing anybody actually grow and eat confirmed cincts.


There was a theory that they look so similar to inactive species that people were foraging the wrong kind and that’s what caused the idea cincts are weak but who knows. 




They're not bad. About the same level as Stuntzii maybe. Back when they were Subbalteatus we used to gather them at the horse stables compost pile. Cultivated specimens were about the same. Nice clean social high.


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OfflineCrimsonRambeler
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: KuroKitsune]
    #28513475 - 10/21/23 08:53 PM (3 months, 5 days ago)

No, they are indeed cincticulus. Yes I was aware of this on some level. I just cooked three NSNS's and three traditional preps, hoping that a grain to grain might save any bacterial contam from ruining the spawn completely. I think there's a bacterial colony I noticed yesterday, leading down to the bottom or I could be just assuming the bottom is stalled from that and hasn't fully colonized. Aside from the possible bacteria I don't believe I have any contams. Only time will tell without a doubt. I think this is gonna be a sick grow. I'll likely grain to grain tmrw or the next day and send to bulk the rest of the grain. Just providing a bit more airy space for the truffles to grow, if it fruits I'll print that, unless cloning is really your guys'es advised next step from a fruiting body.


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OfflineCrimsonRambeler
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: the_chosen_one]
    #28513565 - 10/21/23 11:06 PM (3 months, 5 days ago)

Really? That's interesting. Alot of good information there. Yes I believe that where I'm at way down here by the border I just don't get the ones that pop out of horse stables. It's entirely possible that I just missed them but when they were Subbs I had a horse for about four or five years and I knew what to look for I just never saw them anywhere fruiting directly from horse manure, not one mushroom. I found the one I made the MSS from out in the front lawn of a Hundai dealership. The cap was easily two inches.


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Offlinesleepydave
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: CrimsonRambeler] * 1
    #28513873 - 10/22/23 08:56 AM (3 months, 4 days ago)

Howdy folks, have a couple wild mexicana and possibly the personally sought after Rancho Agosto.  Just chiming in to keep this in my threads!
Much love!


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OfflineFungal-Infection
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: sleepydave]
    #28514853 - 10/23/23 12:00 AM (3 months, 4 days ago)

Hey,

Could I get some advice?

This is my 2nd attempt at Tampanensis from spores.
I streaked 3 plates a week ago and this is what is growing.
The growth started out pure white, then a few days later, this tan colour started to appear in the middle of the growth.

All three plates look the same. White growth turning a tan colour in the middle.

I’ve not done Tampanensis before so I don’t know if this is healthy or if it’s contamination. Could someone take a look at the pics and tell me if this is good or bad please.

Thank you!





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InvisibleReverendMyc

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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Fungal-Infection] * 3
    #28515176 - 10/23/23 10:57 AM (3 months, 3 days ago)

Sorry, I am afraid that your user name checks out. Tamps should not look like that.
At that apparent age they shoold look more like this.


They do turn colors of yellow and brown with age, but more like this.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
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OfflineFungal-Infection
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc]
    #28515184 - 10/23/23 11:28 AM (3 months, 3 days ago)

Quote:

ReverendMyc said:
Sorry, I am afraid that your user name checks out. Tamps should not look like that.
At that apparent age they shoold look more like this.


They do turn colors of yellow and brown with age, but more like this.






In an attempt to help myself while I wait for replies, I was reading through this thread for a lot of the afternoon with much interest, looking for pics of Tampanensis in Agar to compare my plates against and on page 227, Mycolorado posted some pics of some strange looking tampanensis mycelium.

https://www.shroomery.org/forums/showflat.php/Number/25927532#25927532

Pictures 4 and 8 looks similar to the growth in my dishes.

Seeing those pics reassured me a bit but now I’m more confused lol

Any idea what I have growing if it’s not Tamp mycelium because I have 3 plates all growing this stuff.


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InvisibleReverendMyc

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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Fungal-Infection] * 4
    #28515220 - 10/23/23 12:03 PM (3 months, 3 days ago)

I would call it mold and just start a new germ plate. :shrug:

I am not one for short answers but in my agar play book, either it is:
  • healthy looking growth - take a transfer and send to a grain jar,
  • possibly healthy growth with suspicious areas - I need to transfer away from, or
  • just unhealthy growth - that needs to be abandoned and go back a generation or to spores


I don't spend much time on the rabbit hole of which kind of unhealthy growth. I just chuck it and move on. So in that regard I am more of a farmer/gardener than scientist. I don't need to know more about how it failed. I am satisfied with next steps for success.

I do try to figure out where I went wrong and fix that, but knowing the name of the specific microbe that bit me doesn't fix anything. So I focus my energies elsewhere.

tldr: I don't know, but it doesn't really matter which kind of contamination on the journey to success.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Offlinel0wbob
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Fungal-Infection]
    #28515223 - 10/23/23 12:14 PM (3 months, 3 days ago)

What agar recipe did you use for these plates?


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InvisibleMycolorado
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc] * 3
    #28515227 - 10/23/23 12:23 PM (3 months, 3 days ago)

I would agree with reverend except that tamp myc can do weird shit.  This is not to say you’re not growing mold which you could be.  Those cultures you linked to were weird af and produced some good growth that went on to my best grows.  The colored creamy shit never amounted to anything but the more typical growth that would occasionally arise was very good.  Maybe let them grow to see if they give you any additional morphologies like the following. Would also look at using different spores to be safe.


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OfflineFungal-Infection
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado] * 1
    #28515247 - 10/23/23 12:56 PM (3 months, 3 days ago)

Quote:

ReverendMyc said:
I would call it mold and just start a new germ plate. :shrug:

I am not one for short answers but in my agar play book, either it is:
  • healthy looking growth - take a transfer and send to a grain jar,
  • possibly healthy growth with suspicious areas - I need to transfer away from, or
  • just unhealthy growth - that needs to be abandoned and go back a generation or to spores


I don't spend much time on the rabbit hole of which kind of unhealthy growth. I just chuck it and move on. So in that regard I am more of a farmer/gardener than scientist. I don't need to know more about how it failed. I am satisfied with next steps for success.

I do try to figure out where I went wrong and fix that, but knowing the name of the specific microbe that bit me doesn't fix anything. So I focus my energies elsewhere.

tldr: I don't know, but it doesn't really matter which kind of contamination on the journey to success.




I think you’re right. This will be my second attempt from the same print, with the first attempt yielding some funky shit growing too.  Strange brown rhizo type growth with strange truffle looking lumps forming which I was told is probably a slime mold.

I’m going to try and get a MSS from ITW and see if I do better with that. It seems I’ve just been unlucky with this print.



Quote:

l0wbob said:
What agar recipe did you use for these plates?




I didn’t make these plates myself. I got them from a vendor.
It’s MEA with added carbon. I’ve got all the stuff to make my own Agar but I’m a bit apprehensive about doing it since I only have a SAB.



Quote:

Mycolorado said:
I would agree with reverend except that tamp myc can do weird shit.  This is not to say you’re not growing mold which you could be.  Those cultures you linked to were weird af and produced some good growth that went on to my best grows.  The colored creamy shit never amounted to anything but the more typical growth that would occasionally arise was very good.  Maybe let them grow to see if they give you any additional morphologies like the following. Would also look at using different spores to be safe.





Thank you Mycolorado.

Yes, I’m going to try a MSS from ITW.
This is the second try from this tamp print that’s gone tits up.  These are 2 pics of the first attempt. God knows what this stuff is:





Just luck of the draw I guess.

I also had a Mexicana print and that thing grew something that looked like back teeth ha ha

Onwards and upwards!


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InvisibleReverendMyc

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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado] * 1
    #28515264 - 10/23/23 01:22 PM (3 months, 3 days ago)

Quote:

Mycolorado said:
I would agree with reverend except that tamp myc can do weird shit.  This is not to say you’re not growing mold which you could be.  Those cultures you linked to were weird af and produced some good growth that went on to my best grows.  The colored creamy shit never amounted to anything but the more typical growth that would occasionally arise was very good.  Maybe let them grow to see if they give you any additional morphologies like the following. Would also look at using different spores to be safe.




Thanks for the update Mycolorado. I remembered reading that post way back after following the link. I have seen some ugly sclerotia myc before but would have tossed about all of those. So anything is possible, I suppose.

Still, I would recommend sticking to more traditional looking myc like the section you selected in the quoted post. Especially for beginners to stone producers. Certainly no harm in keeping the plate to see if it does something interesting, but I would also start some more to improve the chances of something pretty to work with.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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InvisibleMycolorado
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc] * 1
    #28515282 - 10/23/23 01:31 PM (3 months, 3 days ago)

Agreed.  And I would’ve tossed all those plates had I not grown and printed the fruit that dropped the spores!


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OfflineFungal-Infection
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado] * 1
    #28515875 - 10/23/23 08:23 PM (3 months, 3 days ago)

I’ve just bit the bullet and purchased a tamp syringe from ITW. $50 inc shipping to the UK. :eek:

A bit steep but worth it if they work. :mushroom2:

Thanks for all the help / advice!
Hopefully next time I post, it will be of pictures of healthy Tamp mycelium.


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Offline99.99
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Fungal-Infection] * 2
    #28516721 - 10/24/23 03:23 PM (3 months, 2 days ago)

Going to be lots of stones in this, its from a plate of 2 year old agar



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OfflineA.k.aM
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: 99.99]
    #28516995 - 10/24/23 06:35 PM (3 months, 2 days ago)

What’s up man you take a break????


How long has it been colonized?? Looks pretty potent.


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Offline99.99
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: A.k.a]
    #28517116 - 10/24/23 08:19 PM (3 months, 2 days ago)

Quote:

A.k.a said:
What’s up man you take a break????


How long has it been colonized?? Looks pretty potent.




Oh i haven’t grown anything for the last 6 months , had a death of a very close loved one
Anyways its not even 2 months old . This is the thread it came from its about 2 year old plates i wanted to try out

https://www.shroomery.org/forums/showflat.php/Number/28443365


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OfflineMcShroomface
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: 99.99] * 1
    #28518502 - 10/26/23 04:55 AM (3 months, 1 day ago)

I forgot about those two jars.
They were supposed to be p. tamp.; it was a clean agar culture (T3 from a clone I took from a stone) transferred to rye. They've been sitting in my closet for 15 months now, and I completely overlooked them.
The larger jar doesn't have any stones. The mycelium is turning dark and black in some areas. Is this normal? I recall there was a significant amount of yellow liquid in the jars at one point, but it has since evaporated/ been absorbed. Should I try to proceed with a bulk substrate using the larger jar?


The smaller jar has some stones. I've observed blue spots on the top and I suspect it's mold. Am I right? I believe I opened it a few months back, and since then the jar has dried out, weakening the mycelium and allowing mold to develop. The mold is only evident at the top and not on the sides. Should I still attempt to use the stones, perhaps after boiling them for 10 minutes?


Edited by McShroomface (10/26/23 05:08 AM)


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OfflineYoshiTrainer
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: McShroomface] * 2
    #28518768 - 10/26/23 10:44 AM (3 months, 20 hours ago)

A couple 1/2 gallon jars with SC Tampanensis recovering after G2G. I accidentally made the RGS too wet but should be fine



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OfflineNichrome
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: McShroomface] * 2
    #28518981 - 10/26/23 02:22 PM (3 months, 17 hours ago)

The stones are probably fine. If they have trich or other molds affecting them they are typically soft or necrotic so if you see that, toss those ones in the trash. Stones themselves are very enzyme rich and have pretty good defenses against a lot of molds and other critters.


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