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Offline_OttO_
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Supalemonhaze]
    #23255343 - 05/21/16 06:03 PM (7 years, 8 months ago)

Quote:

Supalemonhaze said:
Well that sucks. I can only get oats here. That or corn which I prefer not to use since they say nutrients aren't as high as others and that the grain will be the only source of nutes for the stones. It's said that bran supplimentation is a good way to help corn out but I reckon that would be disgusting :lol:

I have a 10l bag of rye bran, bought it way back when I made my first mushroom supply buy from a vendor. Thought I was buying grains tbh.




Corn plus coffee might be a winner, to boost up the nutrients. Coffee is highly recommended for all grain soak prep for sclerotia producers anyway, and i also sprinkle a tablespoon of grounds into the strained grain and mix it through before bagging.

And don't worry, Oats are fine - possibly just more labor intensive. It takes me about 2 hours to harvest a bag of sorghum, compared with over 10+ hours to scavenge through a bag of wheat.


Edited by _OttO_ (05/21/16 06:17 PM)


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Offline_OttO_
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: the_r3dz]
    #23255368 - 05/21/16 06:13 PM (7 years, 8 months ago)

Quote:

the_r3dz said:
So OttO, if you don't mind, what is your opinion on this matter

do you find the sclerotia to ingest the grains at all? I noticed that your stones made from sorghum (which is basically a round ball) are much more rounded and look more like little spheres attached to each other, whereas your stones made from wheat/oats/rye (can't remember) Looked more standard and had the oblong connections.

To me, stones look like pieces of grains that have been fused together and devoured, but all the TCs disagree with me. Your post makes me feel even more strongly because the difference between the two actually looks like round connections vs oblong connections - round (sorghum) grains vs oblong (wheat) grains




I do see why you think this, and there is no doubt that the softer the grain, the more the mycelium penetrates the grains (or 'ingests'). I still don't think the grain 'becomes' the Stones, but there is no doubt the softer the grains, the more thoroughly colonized they become and the more entangled the substrate becomes within the sclerotia.

However, the shape of the Stones is probably more due to the shape of the grains they grow around.


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Offline_OttO_
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: _OttO_]
    #23256202 - 05/21/16 10:30 PM (7 years, 8 months ago)

Here is a tray of stones from a wheat bag -



That was about 1/3 of a bag, and took around 4 hours to pick through...  :uhoh:


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Offlinetripdawg420
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: _OttO_]
    #23256217 - 05/21/16 10:32 PM (7 years, 8 months ago)

Quote:

_OttO_ said:
Here is a tray of stones from a wheat bag -



That was about 1/3 of a bag, and took around 4 hours to pick through...  :uhoh:



:homerdrool:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflinePsilosopherr
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: _OttO_]
    #23256220 - 05/21/16 10:33 PM (7 years, 8 months ago)

Got some mexicana jars that have been going a while, got some good sized stones going, but they're drying out noticeably.

Can I just open it up and water it?


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Offlinetripdawg420
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Psilosopherr]
    #23256230 - 05/21/16 10:38 PM (7 years, 8 months ago)

Quote:

rbalzer said:
Got some mexicana jars that have been going a while, got some good sized stones going, but they're drying out noticeably.

Can I just open it up and water it?



prolly time to harvest :thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflinePsilosopherr
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: tripdawg420]
    #23256239 - 05/21/16 10:42 PM (7 years, 8 months ago)

yeah maybe, only been going about 2 months though.


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Offlinetripdawg420
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Psilosopherr]
    #23256250 - 05/21/16 10:45 PM (7 years, 8 months ago)

you have pics :shrug:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflinePsilosopherr
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: tripdawg420]
    #23256391 - 05/21/16 11:33 PM (7 years, 8 months ago)

eh, I mostly wanted to know if watering it would compromise the jar

I'm thinking it should be fine.


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Offlinetripdawg420
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Psilosopherr]
    #23256414 - 05/21/16 11:41 PM (7 years, 8 months ago)

go for it :thumbup: i read people doing it i would start small maybe 3 4 mils :shrug:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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InvisibleSupalemonhaze
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: _OttO_]
    #23256617 - 05/22/16 12:52 AM (7 years, 8 months ago)

Quote:

_OttO_ said:
Here is a tray of stones from a wheat bag -



That was about 1/3 of a bag, and took around 4 hours to pick through...  :uhoh:




Are those dried?

:thumbup:


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Offline_OttO_
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Supalemonhaze] * 1
    #23257238 - 05/22/16 07:21 AM (7 years, 8 months ago)

Quote:

Supalemonhaze said:

Are those dried?




Slightly air died, but not much. Some of them had been drying a day or 2 longer due to the time consuming, staggered harvest process.


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InvisibleSupalemonhaze
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: _OttO_]
    #23259970 - 05/22/16 10:43 PM (7 years, 8 months ago)

I thought they seemed a bit shrunken. Do you grind them down before ingesting? I am just about to inoculate some jars later on today, will be my first sclerotia grow. I might just skip the sclerotia with these first jars and grow shrooms instead, haven't decided yet.


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InvisibleSnazz
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Supalemonhaze]
    #23265362 - 05/24/16 08:49 AM (7 years, 8 months ago)

My ATL has heavily preferred oats over rye. 25 pints and a few quarts, although it is MS.  My gut feeling is that it's due to hulls. 


Less damage when shaking, moisture retention, grain size / space for GE.


Grass seed master also ended up way better.  For ratio, recovery and distribution.


Hope this helps!


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InvisibleNobler Hino
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Snazz]
    #23266658 - 05/24/16 04:15 PM (7 years, 8 months ago)

Interesting thanks


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


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InvisibleStonesoft
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: _OttO_]
    #23267244 - 05/24/16 07:08 PM (7 years, 8 months ago)

Evening fellas. I'm just getting back into this as a hobby after a long break. Grew quite a few PF cakes back in 2012, but then life got hectic and I was on the road for a few years. Was drawn back to this hobby (I swear, watching these little things grow successfully is the most addictive part of all of this) when I finally got a decent place of my own a few months ago, and decided to give sclerotia a go.

I have seven ATL #7 jars of rye currently colonizing (prepared using StoneSun's tek), inoculated on 05/19 with MS from a vendor. Here are three of them with visible mycelium growth highlighted:


Of the other four jars, I saw mycelium on two of them for the first time this morning - not yet worth a picture. The last two currently show no signs of myc at all, gonna give them some time and hope they pull through!

I have another spore syringe on the way, this time from a forum member on a different board, and had a question for you grizzled vets. I don't want to inoculate all of them with MS again, so I'm trying to decide between:
1. Starting with MS on agar and doing Tiger Drop or
2. Creating a mycelium water suspension (wash off mycelium from colonized grain using sterile water, transfer to jar with sterile water, then use to inoc my next batch)

What would you guys recommend? I know agar is currently king, but I'm trying to factor in cost of agar-related supplies plus the fact that my new place isn't the most sterile.. apartment in the heart of a very urban area, and an ongoing mold problem in my radiators. I can create an SAB pretty easily... I guess my main concern with agar is the risk of contamination given my environment, even with SAB. The water suspension seems pretty foolproof as long as I make sure my shit's sterile. Would appreciate any thoughts or recommendations!


Last but not least:

Quote:

_OttO_ said:
On a side note - I'd rather pull my own teeth out than harvest a sclerotia bag on wheat ever again.

It seems that the harder the grain, for example sorghum, the easier it is to clean off the sclerotia - as the grains don't go soft from being so colonised, and the sclerotia form more independently of the grain.

The softer wheat substrate also colonises everytime with bizarre 'veins' of sclerotia running all through it, which is insanely time consuming to try go capture, compared with the solid nuggets that formed in the much harder sorghum spawn.




I think I may get some sorghum growbags pretty soon (which is why I'm gunning for either agar or water suspension), so I'll keep you folks posted with how that goes, assuming it goes.


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InvisibleSupalemonhaze
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Stonesoft]
    #23267389 - 05/24/16 07:45 PM (7 years, 8 months ago)

Your home doesn't have to be sterile, just need to find a room with still air to put your SAB in. I would not trust a homemade spore syringe, regardless of how experienced the owner is.

I would definitely go the agar route because 1 inoculated plate will last for months and even years if you want it to. You can also clone certain strains that give you a higher average yield or potency to increase tge quality of your harvests. Remember that even if you don't see any contams in a spore inoculated plate, always do a couple of transfers to ensure clean growth.


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InvisibleStonesoft
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Supalemonhaze]
    #23267524 - 05/24/16 08:17 PM (7 years, 8 months ago)

Appreciate the quick response, Supa!

Quote:

Supalemonhaze said:
Your home doesn't have to be sterile, just need to find a room with still air to put your SAB in. I would not trust a homemade spore syringe, regardless of how experienced the owner is.

I would definitely go the agar route because 1 inoculated plate will last for months and even years if you want it to. You can also clone certain strains that give you a higher average yield or potency to increase tge quality of your harvests. Remember that even if you don't see any contams in a spore inoculated plate, always do a couple of transfers to ensure clean growth.




Didn't think about higher chance of contams in homemade syringe.. sounds like agar is the way to go, then. I'll get some dishes going and will hopefully be able to cut out any unwanted intruders. Guess this will finally push me to try cloning, which just seems so damn intimidating, but what the hell eh.

I've always wondered about storing plates long-term: do you just store them in the fridge/freezer? I didn't know they can keep for years - what about senescence?


Edited by Stonesoft (05/24/16 08:19 PM)


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InvisibleSupalemonhaze
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Stonesoft]
    #23268581 - 05/25/16 03:17 AM (7 years, 8 months ago)

Senescene gets put on hold in cold storage. Plates can be kept for a couple of months, probably more but if you want long term storage, master slants is definitely the way to go. Also, cold storage is done in a fridge, exposed mycelium is not able to survive freezing. When  you use a piece of wood like the tek I linked, cultures are able to survive a couple of years in slants during cold storage. It is always recommended to make a new slant before 2 years though, just in case. Without the wood, slants are said to last a whole year less.

Cloning is just as easy as a spore inoculation. Just grab the best looking shroom from the biggest cluster and prep your SAB. Use your hands to manually split the shroom down the middle and rub your flame sterilized scalpel in the newly exposed tissue until you get a piece. Transfer that to a plate and treat it like any new culture, that is to make a couple of transfers to ensure cleanliness. It is actually easier to clone than to get healthy mycelium from a spore inoculation since the clone is already alive. A lot of times, molds and bacteria are able to grow before your mushroom mycelium when using spores so you have to clean them up. A clone normally grows enough to take a transfer before any molds are able to germinate. I find bacteria is more common with molds but they are easy to get rid of.

Some vendors go to extra trouble to make sure the syringes/prints they supply you are super clean yet even with a vendor bought syringe, sometimes you come across contams. Homemade prints can be rather clean but going from print to syringe is an extra step where something is bound to end up in your solution. I have yet to use a syringe straight to grains, be it vendor and homemade. I started with agar from the get-go and I am very pleased with it.


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OfflineSivarted
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Supalemonhaze]
    #23273386 - 05/26/16 10:22 AM (7 years, 7 months ago)

So I have some jars that I have neglected since october, and am concerned that the stones might have gone bad.  How would I know?


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