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Genome794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Adas]
#28155555 - 01/24/23 01:28 PM (1 year, 3 days ago) |
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Time for me to join here..
I acquired a syringe marked "Psilocybe Mexicana var Galindoi ALT#7 2.0". The local supplier I decided to try seems to be unreliable. Plates all seemed to have yeast but an oat-puck got going okay.
I transfered several grains to new plates and LC's... The plain water LC produced a lot myc quickly. So, I noc'd an mini jar of oats.
I am hoping to bring this grow full circle... All the way through collecting stones, fruiting and making prints.
-------------------- Looking at myc on agar never gets old. LAGM-2023 Bigfootscreepyuncl - LAGM2023 - Challenge
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Genome794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: A.k.a]
#28156942 - 01/25/23 12:22 PM (1 year, 2 days ago) |
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Quote:
A.k.a said: I wouldn’t have much faith in a vendor who doesn’t even know what species they’re selling.
Plain water shouldn’t be growing much either.
100%, hindsight is 20/20. I was trying to support a home team. I have found complaints about this vendors quality on this board. So, some school fees.
Agreed re growth "on water". Allow me to clarify. I took a single myc coated grain to approx 8ml sterile water. The idea was to dislodge myc from grain by violent shaking and streak later in the hope of cleaning up the culture.
I assume that metabolites and nutrition moved from the grain to the water, to balance concentrations and the dislodged myc grew on that.
I transfered 2ml of that to... Call it a starter jar containing 3 tblspn oats. If I am lucky, I get a better ratio of myc over contam. Repeat until I can get a good plate.
-------------------- Looking at myc on agar never gets old. LAGM-2023 Bigfootscreepyuncl - LAGM2023 - Challenge
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Genome794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: LtLurker] 1
#28163811 - 01/30/23 05:13 AM (11 months, 22 days ago) |
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Quote:
LtLurker said:
Quote:
Genome794 said:
Quote:
A.k.a said: I wouldn’t have much faith in a vendor who doesn’t even know what species they’re selling.
Plain water shouldn’t be growing much either.
100%, hindsight is 20/20. I was trying to support a home team. I have found complaints about this vendors quality on this board. So, some school fees.
Agreed re growth "on water". Allow me to clarify. I took a single myc coated grain to approx 8ml sterile water. The idea was to dislodge myc from grain by violent shaking and streak later in the hope of cleaning up the culture.
I assume that metabolites and nutrition moved from the grain to the water, to balance concentrations and the dislodged myc grew on that.
I transfered 2ml of that to... Call it a starter jar containing 3 tblspn oats. If I am lucky, I get a better ratio of myc over contam. Repeat until I can get a good plate.
For whatever its worth; I respect trying to help a (small?) Local guy. Your process to dilute and streak for a cleaner sample instead of going straight for growth is a good plan with some reasonable thought behind it.
Check out brf pucks. Good tool to nerf bacteria growth in contaminated samples. You might get some use out of those with this project if you can work off the molds and yeast but still have bacteria issues.
Whether it works out this time or not, you're gonna do great going at it like this and putting some solid thought into your problems. Keep it up dude, you got this.
Thanks LtLurker...    
The clean up work is a relentless endeavour. One has to keep going until one is gets that one plate with just enough myc just far enough away from the contam... Or plain luck. Then the prayer for fruiting genetics begins.
But as you are a TC. I believe you know, you know.
-------------------- Looking at myc on agar never gets old. LAGM-2023 Bigfootscreepyuncl - LAGM2023 - Challenge
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Genome794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Adas] 2
#28167655 - 02/01/23 02:04 PM (11 months, 19 days ago) |
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I seem to have got somewhere with my bad syringe.
First plates and a mini jar were noc'd on 8-Dec... Yeast on all plates. Similar to this.
 Dumped 'em.
The jar took forever to show signs of growth. I let it grow until I say grains going black. 2 rounds of taking colonized individual grains to plates. Green followed in the jar... Took a chance and did some careful open air transfers of individual grains to plates. Still yeast everywhere but managed to scrape nearly invisible bits of myc off the grains or plates and...
 Nearly 8 weeks and dozens of plates later... I am satisfied that I can make good in joining in growing sclerotia.
I know the tubs are not clear and you cannot really see the myc properly. Atleast they are uniform, fairly organised and look like one type of myc.
-------------------- Looking at myc on agar never gets old. LAGM-2023 Bigfootscreepyuncl - LAGM2023 - Challenge
Edited by Genome794 (02/01/23 02:08 PM)
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Genome794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: FlawdaKraka] 1
#28181852 - 02/11/23 07:23 AM (11 months, 9 days ago) |
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Quote:
FlawdaKraka said: Mexicana A T1 plates, gonna make a couple transfers today, but would like to hear opinions on if these are mushroom mycelium? First go around with this print turned green on me.



Damn, I am jealous... In a good way. I am still on my journey of cleaning up a culture.
I tried asking in the big contam thread about my progress but only rumfor69 came back with his opinion - but he isnt sure about sclerotia formers. So, I apologise if this counts as a cross post.
A few pages back I mentioned the name given by the vendor and I think I can still hear the laughter. Now the only way to get an idea of its identity is to grow it out.
After trying something different I choose to do a hot agar pour - Feb-3. One plate may have given up something. Feb 8
 Not knowing what I am looking at I took a few transfers from the colony at 2 o'clock. (Something brown growing under surface after 3. Yeast 3-5. Some cobweb/white mold 10-11.)
Feb-11 - Same plate
 Apologies, I did think to check the colony positions when I took the pic. So, the cobweb/mold is now at 9. Dark blob at 1-2 and everyone can see the yeast. Lastly, my hope hangs on the thing that is now at 11-Noon and heading south.
Your opinion? I just finished making a new batch of BRF pucks. So, if it isnt an ATL/Galindoi/Tampanensis myc, I am ready to start again.
-------------------- Looking at myc on agar never gets old. LAGM-2023 Bigfootscreepyuncl - LAGM2023 - Challenge
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Genome794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: scapo] 1
#28254884 - 03/30/23 03:16 PM (9 months, 24 days ago) |
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Hey Sclerotia Growers,
Need the help of a practiced eye here.
Been working on cleaning a culture from a bad syringe. These are the closest I have come to what might be the correct mycelium.
  It doesn't seem keen on the activated charcoal agar... Th other plate is my version of PDA.
The first plate, 6 days along

And these are the best two from the transfers. From the charcoal agar

From the PDA

There are two baby-food jars with oats in that got some agar as well. Myc is thin but not too far from some of the pics on this thread. Still it is just so wispy that I keep thinking it is cobweb, albeit a slow one.
Thanks for your time. Just need to know if I can continue to transfer or go back to the syringe for a last round of small BRF pucks.
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Genome794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Pluviophile]
#28259264 - 04/02/23 02:30 PM (9 months, 21 days ago) |
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Thank you Pluviophile and Milkboy. I am used to considering myc that looks like that to be bad.
Pluviophile, I have taken more plates from that second one and committed some agar from the first one to grain in baby food jars. The mini-jars have... Say 3 tablesppons of oats in.
I am very excited to get this variety going as I have never consumed sclerotia before. Thanks for your feedback.
G
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: folder52] 2
#28264962 - 04/06/23 09:54 AM (9 months, 17 days ago) |
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Hello again,
Just short of two weeks on. I have two types of plates. This looks more regular to me.
  I took T2's from the first plate at 1-ish, 3, 4:30-ish and 7:30-ish. Also took what was left of the top half to a mini-jar (only the myc, not the open agar).
And then this.
 The white balance or something makes it look much thicker/easier to see than it is.
I am holding onto all of them at the moment because of my complete ignorance when growing these. (Except the obviously contaminated ones.)
Looking at the previous round's mini-jars. The myc looks Cobweb-like to me.
 
I have one plate, similar to the first one that could have a stone forming - here's hoping.
Comments and suggestions are welcome even desired.
Thanks for your time G
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: kirkeng] 1
#28270941 - 04/10/23 10:22 AM (9 months, 13 days ago) |
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Thanks for the help so far.
To continue the learning... After going through about 50 of the early pages of this thread. I think I know what my plates and jars should look like but I am not confident yet. Last request for a pat on the back, promise (until I get to fully colonised jars).
Agar - 3% MEA. Malt extract is a syrup but not for beer brewing. Apologies for taking pics through the lids, I will take the pics in my SAB from here on if the feedback on the plates is still good.
To my understanding, this plate is good? This plate was noc'd on March 24 - T1.
 The myc is wispy and spreads fast across the plate. 6 days from noc. The myc seems to thicken after. On my plates, the myc thickens where the plate is thicker.
Again, a good plate? This one, noc'd on March 31 - T2.
 The myc farther out Has not started to thicken but it is definitely more aerial.
Once more? Noc'd on April 6 - T2.

Mini-Jars - Oats (not a good bag, had a lot of wheat, some small black beetles, even a few pieces of millipedes). This is the oldest jar. I think I was just doing Noob stubborness and rushing but... I got lucky, I think.
  The myc has thickened up.
This jar got a piece of agar on March 31.
 This is the wispy myc before thickening (I hope). The entire visible area of the jar is colonised.
This mini-jar got a wedge on April 6, from a T1 - because I can.

Jars - 350ml - Oats. I have three jars like this. Again, it looks good to me.

Anything look off here?
Thanks again.
-------------------- Looking at myc on agar never gets old. LAGM-2023 Bigfootscreepyuncl - LAGM2023 - Challenge
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Genome794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Throwaway]
#28309204 - 05/06/23 02:03 PM (8 months, 18 days ago) |
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Just sharing progress... And a question or two. ( I am 130-something pages into the thread... I might start skipping pages soon )
Getting stoned

With a friend jars...
 1st Q. Is it too late to move these to bulk?
One of each of the jars in the group. I have 6 of the one on the left - they will be left as they are until stone harvest day. 2 of the middle size - gonna do bulk stones and hopefully fruit. 4 of the small jars - also bulk stone and fruit.

This is a 5 quart monotub, just under half full. Is now the time to case it?

Thanks for all the help so far. Any other comments welcome.
EDIT: Also, I have a bag, half colonised. I am keen to mix it up.
794
Edited by Genome794 (05/06/23 02:18 PM)
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Freshpana1936]
#28327605 - 05/20/23 01:52 PM (8 months, 4 days ago) |
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Straight brown rice... My culture grew so fast before I knew it I had like 20 plates - after making long-term jars and a bag and more jars. I'll give it a go. Thanks MacMerdin - and Freshpana for asking the question.
For this part of my learning-curve I went straight to boxes. Spawn was oats - bulk was sorghum(coffee soak and boil) and coir. Ratio 1:2.
 This was fully colonised in 10 days and cased with standard 50/50+ on May 7 - so, 13 days.
 Same spawn and bulk. Went to bulk on May 7 and was cased immediately.
Both tubs are 5 quart, unmdified. first box is half full the 2nd, 3/4 full. Do they look like they are supposed to? Where to from here? Double tub them? Just crack the lids?
(weather is a problem at the moment - Average temp in my grow room is 16C.)
Thanks in advance (and all the help so far).
Edited by Genome794 (05/20/23 02:18 PM)
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado]
#28328196 - 05/20/23 11:45 PM (8 months, 4 days ago) |
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Quote:
Mycolorado said:
Quote:
Genome794 said: bulk was sorghum(coffee soak and boil) and coir. Ratio 1:2.
This sounds problematic. Growth is super fuzzy…if you spawned to sorghum, coffee and coir without doing so in a sterilized bag, it’s gonna blow up, and not in a good way. What exactly was the process?
Damn, that was some choice communication by me...
Thanks Mycolorado.
Fixing bad comms. Bulk was coffee-soak-sorghum and coir at 50/50 with a random amount of gypsum. 750ml jars. Soak approx 12 hours, 5 minute boil, hour or so drain. Coir at field capacity. Pasteurised for 2 hours between 60C(160) and 68C(170?).
Spawn ratio 1:2.
I have a journal - not complete - with other pics of plates and jars. It isn't "War and Peace". If you have the time.
Thanks again.
794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado]
#28328893 - 05/21/23 02:32 PM (8 months, 3 days ago) |
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Quote:
Mycolorado said: Still not following. Did you pasteurize the sorghum? If so, that’s not gonna cut it. Sorghum should be used as the spawn, but you said the spawn was oat. That’s what’s confusing me. If you are going to put a grain in the sub, it needs to be sterilized.
Sorghum went to PC first. I didnt think to mention it because I assumed everyone sterilised grains first. I would have had a crop of sorghum sprouts by now.
Thanks Mycolorado.
I guess it is back to "wait and see". No odd smells. The one tub smells more mushroomy than the other. I am going to have to mist at some point the surface is starting to look dry.
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado]
#28329397 - 05/22/23 12:31 AM (8 months, 3 days ago) |
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Quote:
Mycolorado said: If you put sterilized grain that hasn’t been colonized in a tub, you are going to have problems.
Quote:
Mycolorado said: If you are going to put a grain in the sub, it needs to be sterilized.
And by this, I mean the sub with grain would be sterilized and then inoculated and and allowed to colonize under sterile conditions, as in a grow bag.
  Got it... (Too much reading across too many threads.)
Uncolonised grains are a nutrient source. Other ingredients will have other bacteria. Even after pasteurisation the rule of first arrival applies. The leftover bacteria, albeit beneficial, will colonise the resource and probably move the pH down and give others a head start on the mycelium...
Other sub recipes don't have such rich nutrients sources or no-nutrient resources for anyone other than the mycelium... So, they can be pasteurised.
There will be exceptions to the above but its the starting point for consistent results.
Thanks for the patience My Colorado (and everyone else for sitting through my learning).
794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: MacMerdin] 1
#28339489 - 05/29/23 01:41 PM (7 months, 26 days ago) |
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A progress update...
Had a pack of 300ml ATL7 jars. The first 5 went to these 1.5L glass jars - going for stones. G2G Spawn ratio 1:2 News grains are Sorghum and oats - PC'd for 2 hours @ 15 PSI. Jars packed on May 27 1 Day
 Could hardly see the grains on day 0.
2 Days

The remaining 4 jars I am going to try for fruit directly. They went to bulk today.

1 box(5 quart) and 2 bottles(1 liter)
 Sub is Coir/vermiculite/cow dung - 2:0.5:1 - Pasteurised 3.5 hours. Spawn ratio 1:2
I know my process looks erratic but I am just trying stuff to see what happens. I am 160 pages into the thread and still unpacking/sorting information.
As usual, comments/advise/observations welcome.
(And thanks for all the help so far.)
Edited by Genome794 (06/01/23 06:08 AM)
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: csilocybepubensis]
#28339504 - 05/29/23 01:49 PM (7 months, 26 days ago) |
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Nice fruit.
Thanks for the idea of putting the tray into a tub. Cant believe I didnt think of that.
Got to try that.
Thank you.
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Genome794] 2
#28345889 - 06/03/23 02:36 PM (7 months, 21 days ago) |
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Going well with the bigger jars
4 days - May 31

7 days - June 3

The myc's colonisation speed of ATL7 is amazing to me. And at average day time temps of 16-18C in the grow area.
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: csilocybepubensis]
#28346582 - 06/04/23 02:52 AM (7 months, 21 days ago) |
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Quote:
csilocybepubensis said: Oh yeah man them tamps are speedy in all aspects. You gunna fruit those jars?
Those are for stones Of course after stone harvest, I will try for fruit as well.
I have 3x 5 quart tubs going for fruit. Two are already cased.. When the third one is ready for casing, I'll try putting them all in a tub with a fish tank aeration stone.
The culture seems to be stone-driven. Could be my current grw area temps - 16-18C by day. I am at pg 160 of this thread (or it was in the last two weeks) that I read low temps favour stones and warmer temps favour fruit.
Can anyone confirm?
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado]
#28403528 - 07/22/23 04:44 AM (6 months, 4 days ago) |
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@SirPsycho. Nice work/good job.
My current effort with these genetics
  Just clean looking stuff at 2.5 Months.
 These two have me wondering. I doubt you would be able to see. The mass is not individual stones like the others. It started as tiny (pin-prick sized) beads and the number has just grown and taken over the visible space. Anyone know if this is an indicator if these will be better at fruiting?
 I have read and seen in others pics that discolouration is normal... But pink? I suspect lipstick mold but this far along?
Half a cross-post.
 My blue oyster syringe might be mislabeled. New plates will show if it was me that mislabeled plates.
The reason I believe it isn't me... It would be the first time in over several hundred plates - it i spossible. But these are 3 weeks old and changing colour (on sorghum), my ATL7 above is 2.5 months old (on Oats) with no colour change.
As always, great to be a participant in this community. To see other's great and inspirational work. Thank you.
Any comment/advice welcome. 794
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado]
#28407323 - 07/25/23 12:39 AM (6 months, 1 day ago) |
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Quote:
Mycolorado said: None of them look good imho or at least not what I would expect from tamps. Definitely toss the pink jars.
Thanks Mycolorado.
Bugger and there I thought I was doing okay.
Syringe from an Upside-down vendor, labeled "P.Mexicana var. Galindoi (ATL7 2.0)... So, yeah, for all intents and purposes, a Tampanensis - I hope.
When I get over my Noob-selftalk about how much grain and time I have invested in the remaining jars... I will top-fruit some and put the others in a tray.
I have read that this family like warmer temps. Any point in trying to fruit them below 18C? (My current ave is 15C)
794
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