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Offlinetree frog
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Registered: 09/14/23
Posts: 442
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: rsssk]
    #28490815 - 10/03/23 09:50 AM (3 months, 23 days ago)

21 days from LC is a long time.  (If you meant MS I'd let it finish colonizing).

And the growth looks more like mold to me than any sclerotia species I've worked with (mex, tamps, and the atl 7 strain).

Final thought is I see no signs of stone production.  Granted that won't often happen until after full colonization.  But I have seen small stones in partially colonized jars and if ot had stones, we'd know for sure it's the right mycelium and not a mold.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Edited by tree frog (10/03/23 09:52 AM)


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Offlinetree frog
eats bugs

Registered: 09/14/23
Posts: 442
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Telomere]
    #28495199 - 10/07/23 07:43 AM (3 months, 19 days ago)

I have some ATL 7 going from MS (Shroomery sponsor who's always given me clean spores).

Was thinking of going from half pint millet (what I germinated on) to GLC and then to rye grass seed (in bags).  Let the donar jars recolonize for stone production and cloning.

Or I could grain to grain but then I'd have to pick millet out later?

Any thoughts?


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
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Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: THE MYCOSMITH] * 2
    #28521103 - 10/28/23 12:24 PM (2 months, 29 days ago)

ALT 7 inoculated from vendor spore syringe about four weeks ago.

When and what should I clone?



The large stone in this picture was the first to develop.  So, I'm certainly looking at this one to clone.



The other side of the jar has some nice clusters happening.  Clusters were slower to develop than the large stones on the other side.  I remember reading someplace that cluster clones give better yields though?

So, any thoughts?  The large stone is about the size of my thumb.  So I could clone it now without trouble.  The clusters are much smaller but probably doable, especially if I wait a week.

Any thoughts or feedback appreciated.  I think having typed this I'll wait at least a week to crack it open and make clones.  Clone a couple big ones and a few clusters.  But this is my first time cloning stones so any advice would be helpful.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
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Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado]
    #28522014 - 10/29/23 07:49 AM (2 months, 28 days ago)

Quote:

Mycolorado said:
Nice!  Clone the big ones.  Fruit the grain.




Thanks!

I took six tissue samples from three seperate sclerotia (the big one, the nice cluster, and another big one that was hiding in the center).

Going to try fruiting on just CV top dressed and compressed in a dubtub with a few extra holes at substrate level.  I've had moderate success years ago with Tamps/Mexicana fruited like this without cloning.  Not ALT 7 specifically though.  I know it's not the best fruiter.

If anything comes of it, I'll clone it!

Got 16 wet grams of stones.  Not bad for a 3 cup jar MS jar and only four weeks.

Also, rye berries from now on.  Fuck picking millet out of stones!


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Edited by tree frog (10/29/23 07:49 AM)


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
Loc: lives in trees
Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: rockyfungus]
    #28541135 - 11/13/23 05:57 AM (2 months, 14 days ago)

I have some ATL 7 pinning and was wondering what a good strategy for cloning fruit is with how small and fragile they are.

Should I try dropping pins straight onto 1% or water agar?  Which would be best?

Or should I fuck around with trying to get inner tissue?  Or wait for a stone to both break the surface and fruit, and hope those fruiting genetics are in the stone?


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Telomere] * 1
    #28541263 - 11/13/23 09:04 AM (2 months, 13 days ago)

Thanks for that advice.  I've cloned from this spot with other species so I know what you're referring too.

I'll let them mature some and give it a shot!


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc] * 1
    #28541513 - 11/13/23 01:45 PM (2 months, 13 days ago)

Are they still worth fruiting aside from the novelty then?  I like the sclerotia a lot anyway.

Debating if I'll bother cloning as my pan cultures seem to be colonizing.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
Loc: lives in trees
Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: ReverendMyc] * 1
    #28541648 - 11/13/23 04:13 PM (2 months, 13 days ago)

Thanks for the responses.

I've only done the sclerotia twice recently but a few times before and a lot of cubes and other psychedelics.  I found my recent sclerotia experiences to be clear headed enough to meditate on, which took me out past the dark clown territory.  There was a lot of that energy on the come up though.  Luckily I was listening to a dharma talk on looking deeply and when I started looking into the dark clowns, which were the five hindrances in my understanding, I saw how they're made up of everything else and they started to come apart like spaghetti.

Cubes I can get confused more easily it seems, which makes it harder to remember how to calm myself.

So alright, I'll look at the fruits in their own right.  It sounds like they might be a lot more grounding than cubes which I would appreciate too.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Adas] * 5
    #28542421 - 11/14/23 09:05 AM (2 months, 12 days ago)

That's sick!  I've never even seen an agar pin.





A couple of decent ATL 7 clusters to clone.  I'll probably take a few tissue samples from both of these pictures.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: l0wbob]
    #28546677 - 11/17/23 03:40 PM (2 months, 9 days ago)

I have a bunch of half gallon jars.  I remember RR used to say they go aenerobic?

Are you doing anything special with your gallon jars as far as GE?


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: l0wbob]
    #28547452 - 11/18/23 07:11 AM (2 months, 8 days ago)

Awesome explanation, thanks!

I have some lids I can modify with a few extra GE holes and I'll give it a try once I get an LC working.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
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Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: CocaineBuffet] * 9
    #28548795 - 11/19/23 07:07 AM (2 months, 7 days ago)

Have taken around twenty tissue samples from five or so separate fruits or clusters of fruit over the last week.  All from a nicely fruiting ATL 7 MS tub.  Splitting the fruits and scraping the inside gently seemed to work the best for me.

Said tub.



Hopefully I'll be posting some canopy pictures beginning of next year!


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Edited by tree frog (11/19/23 07:10 AM)


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Offlinetree frog
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Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: l0wbob] * 1
    #28549816 - 11/20/23 04:58 AM (2 months, 7 days ago)

Pulled a single fruit with a decent sized stone attached to it and cloned it.  I'm hoping it will both stone well invotro and then produce a decent canopy if I spawn it.

Excited to see what it does.  I probably pulled fifty fruits from the first flush before seeing a stone pop out.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
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Registered: 09/14/23
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: normalperson] * 1
    #28551437 - 11/21/23 11:15 AM (2 months, 5 days ago)

It looks clean to me and if you've shaken it as much as you say I would be surprised if it bounced back that well if it was contaminated.

But most of my growing experience comes from edibles so you may want to wait for someone more experienced to chime in. (which happened while I was babbling...)

But I would use that jar for g2g personally, especially between the shaking and it coming from agar rather than MS.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Edited by tree frog (11/21/23 11:15 AM)


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Offlinetree frog
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Registered: 09/14/23
Posts: 442
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: YoshiTrainer] * 2
    #28551915 - 11/21/23 06:25 PM (2 months, 5 days ago)

I have a masticating juicer I sometimes make horseradish with.

Was thinking I could pulp stones with it, and either dry like a fruit leather, or soak the mash in some very hot acidic water for ten minutes, strain through a nut milk bag, and freeze into ice cubes to save for later.

Basically I like stones but am not in love with dry stones.

Any thoughts on if the above would be an effective storage method?


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Pluviophile]
    #28552394 - 11/22/23 04:49 AM (2 months, 5 days ago)

yeah, I realized last night after I posted if I grind them wet for drying I'm going to lose potency.

I'll run some through it wet and make tea with it.  And maybe see how it does as a grinder with a few dry stones.

What kind of grinders are you all using for dry stones?  I remember reading years ago that they'll kill cheap coffee grinders pretty quick.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
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Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: GC3DTC] * 3
    #28554647 - 11/24/23 04:54 AM (2 months, 3 days ago)

I've been toying around with the idea of a non-nutritious casing layer that's easier to get stones out of then peat.

Last night I realized I could probably cut 90 shade cloth to cover grains and then cover the shade cloth with a regular 50/50 casing.

Should produce a casing I can easily lift out and the mycelium should be able to colonize up through the shade cloth.

Research I've read on morels suggests that it likes to form stones between a nutritious and non-nutritious layer.  This seems similar to how Tamps behave.

So the hope is the stones will form under the shade cloth, and bulk stones would then require little more cleaning then invitro stones.

I'm going to run a few experiments on it and will report back in a month or so with results.  But I'm curious if anyone has tried something similar or seen something similar I could look at.

Thanks!


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Advil_Liquigels]
    #28559936 - 11/28/23 07:05 PM (1 month, 29 days ago)

Tea is fine.  Vermiculite is not toxic.  Just not fun to chew on.  It's a form of mica.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
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Last seen: 1 hour, 37 minutes
Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: normalperson]
    #28565206 - 12/02/23 12:29 PM (1 month, 25 days ago)

Saw some green on one of my stones this morning so harvested the bag early.  Not even half a dose.

I think I'm sticking to jars.

Anyway, question.

Can sclerotia bruise green?  I saw no other signs of trich and plan to toss the grains in a shoebox to see what they do.

But I'm kinda feeling like I may have freaked out over bruising.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


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Offlinetree frog
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Registered: 09/14/23
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Re: The OFFICIAL Stone producer/sclerotia thread and discussion [Re: Mycolorado] * 1
    #28566587 - 12/03/23 10:43 AM (1 month, 24 days ago)

I learned yesterday that sclerotia contain trace amounts of maois and will floresce under a UV light like harmaline.

Unfortunately my phone refuses to get a decent picture with my little UV flashlight shining on the jars.

But I got some work done today and added five more half gallon jars to my stash.

These are all ALT 7.  Mostly an aggressive MS plate but there's a couple of clones in there.  One sclerotia that came up with a fruit.  I'm curious how it will do in jars but might go straight to bulk with it.  And some fruit clusters that will go straight to bulk.


I also supplemented one of the MS jars with a gram of mannitol and a half gram of brewer's yeast.  There's been studies on morels and apparently these two ingredients are preferred by morels for sclerotia formation.  I've been curious if Tamps will like it so running this test jar as well as some plates with the same ingredients (swapped half my LME for mannitol on the plates).



Nine half gallon jars total.

It's going to be a happy new year for my friends and I.

The MS culture, about four weeks old.



All that yellow glows green under a UV light.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Edited by tree frog (12/03/23 10:51 AM)


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