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Amazon Shop: ½ Pint Jars, Pressure Cooker, Rye Grain

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Invisiblesnatchcakes
Rad Dude

Registered: 01/17/04
Posts: 714
Loc: HellTrack
Rye Grain/fractional sterilization
    #2250781 - 01/18/04 05:04 PM (14 years, 4 months ago)

I do not own a pressure cooker.I wanted to try rye along with PF for my first grow so i tried this.
I soaked for about 16 hours,rinsed,let drain for about 10 minutes,loaded in jar With polyfill lid,steamed for about 40 minutes,let set over night,next day steamed for about 35 minutes,let sit overnight,3rd day steamed about 70 minutes,let sit overnight,next day innoculated with GTs,shook to mix up spores coating all rye.
Question #1-was this done correctly?
#2-Is this a reliable method or not
#3- if the jar was going to contaminate,how long would it be before you could safely say it probably doesnt have any contaminants?
thank you for your help.


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InvisibleBadEnglish
Chief Of Staff

Registered: 12/03/03
Posts: 369
Re: Rye Grain/fractional sterilization [Re: snatchcakes]
    #2251186 - 01/18/04 08:09 PM (14 years, 4 months ago)

Heres a link to a tek on it,i dont know if youve read it or not,ive never tried so i personly cant help.
http://www.shroomery.org/index/par/23372


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Invisiblesnatchcakes
Rad Dude

Registered: 01/17/04
Posts: 714
Loc: HellTrack
Re: Rye Grain/fractional sterilization [Re: BadEnglish]
    #2251334 - 01/18/04 10:23 PM (14 years, 4 months ago)

thats the tek i did but i just wanna ask someone who has actually tried it.thank you though.


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Offlinetrkgsy
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Registered: 12/17/03
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Re: Rye Grain/fractional sterilization [Re: snatchcakes]
    #2252099 - 01/19/04 06:27 AM (14 years, 4 months ago)

Anno the wise said it worked for him. Should be effective for ALL endospores if done properly, but then I'm not experienced


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OfflineMAIA
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Re: Rye Grain/fractional sterilization [Re: snatchcakes]
    #2252603 - 01/19/04 01:52 PM (14 years, 4 months ago)

Quote:

1-was this done correctly?




Maybe a bit overcooked but i think that's ok.

Quote:

2-Is this a reliable method or not




If done correctly, yes.

Quote:

3- if the jar was going to contaminate,how long would it be before you could safely say it probably doesnt have any contaminants?




If you don't see germination in 1 week or 2, then i would start worrying. If it germinates and it takes too long to colonize - more than a month - then maybe, you could be dealing with some problems too.
Good luck !

MAIA


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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Invisiblesnatchcakes
Rad Dude

Registered: 01/17/04
Posts: 714
Loc: HellTrack
Re: Rye Grain/fractional sterilization [Re: MAIA]
    #2252620 - 01/19/04 02:00 PM (14 years, 4 months ago)

First thank you for the help.
Second I do see growth.This morning after 4 days i noticed about 10(that were visable anyway)grains that had myceluim growing on them!My PF jars are on there 10th day before i saw growth.And im glad i did it right.Thanx again all.oh yeah the containers are 1/2 pint so this shouldnt take long to fully colonize right?especially at this rate.  :grin:


Edited by snatchcakes (01/19/04 02:03 PM)


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Amazon Shop: ½ Pint Jars, Pressure Cooker, Rye Grain

Mushrooms, Mycology and Psychedelics >> Contamination Forum

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